scholarly journals The Level of Leukocytes, Eosinophils, Monocytes, and Lymphocytes in Mice (Mus musculus) on Post-Inoculation of Trypanosoma evansi

2018 ◽  
Author(s):  
Marek Yohana Kurniabudhi ◽  
Era Hari Mudji ◽  
Rini Fajarwati
Keyword(s):  
Mus Musculus ◽  
Trypanosoma Evansi ◽  
Post Inoculation ◽  
Monocytes And Lymphocytes

scholarly journals Parasitemia Level of Male White Mice (Mus musculus L.) DDY Strain Infected with Trypanosoma evansi of Pidie and Pemalang Isolate

2016 ◽  
Vol 9 (2) ◽  
Author(s):  
Febiola Rama Sari ◽  
Yudha Fahrimal ◽  
Ummu Balqis ◽  
Didik T. Subekti ◽  
Aprilia Wardana ◽  
...  
Keyword(s):  
Mus Musculus ◽  
Trypanosoma Evansi ◽  
Rapid Rise ◽  
Tail Vein ◽  
The Third ◽  
Parasitemia Level ◽  
Short Time

This study aimed to observe parasitemia of DDY white mice infected with T. evansi of Pidie and Pemalang isolates obtained from Balai Besar Penelitian Veteriner (BBPV) Bogor. Twelve DDY mice were divided into 3 groups, each group consisted of 4 mice. Mice in the first group were not treated with any treatment. The second group was inoculated with 104 T. evansi Pidie isolate and the third group was inoculated with 104 T. evansi of Pemalang isolate. Mice blood were collected every two days from tail vein for parasitemia. examination Parasitemia examination was conducted until all mice die. The results indicate that there was difference in parasitemia level between the two isolates. Parasitemia of mice infected with Pidie isolate characterized by rapid rise of parasitemia in blood (107-108/mL of blood) in a short time (2-4 days) since first parasitemia was detected and followed by death at day 4. While the parasitemia of mice infected with Pemalang isolate increased in the blood (108-109/mL of blood) on day 4 and maintained for a few more days and then fluctuated for a few more days before the animal died.Key words: Trypanosoma evansi, parasitemia, pidie isolate, pemalang isolate

scholarly journals TINGKAT PARASITEMIA MENCIT PUTIH (Mus musculus L.) JANTAN GALUR DDY DIINFEKSI Trypanosoma evansi ISOLAT PIDIE DAN PEMALANG (Parasitemia Level of Male White Mice (Mus musculus L.) DDY Strain Infected with Trypanosoma evansi of Pidie and Pemalang Isolate)

2015 ◽  
Vol 9 (2) ◽  
Author(s):  
Febiola Rama Sari ◽  
Yudha Fahrimal ◽  
Ummu Balqis ◽  
Aprilia Wardana ◽  
Muhammad Hambal
Keyword(s):  
Mus Musculus ◽  
Trypanosoma Evansi ◽  
Rapid Rise ◽  
Tail Vein ◽  
The Third ◽  
Parasitemia Level ◽  
Short Time

This study aimed to observe parasitemia of DDY white mice infected with T. evansi of Pidie and Pemalang isolates obtained from Balai Besar Penelitian Veteriner (BBPV) Bogor. Twelve DDY mice were divided into 3 groups, each group consisted of 4 mice. Mice in the first group were not treated with any treatment. The second group was inoculated with 104 T. evansi Pidie isolate and the third group was inoculated with 104 T. evansi of Pemalang isolate. Mice blood were collected every two days from tail vein for parasitemia examination. Parasitemia examination was conducted until all mice die. The results indicate that there was difference in parasitemia level between the two isolates. Parasitemia of mice infected with Pidie isolate characterized by rapid rise of parasitemia in blood (107-108/mL of blood) in a short time (2-4 days) since first parasitemia was detected and followed by death at day 4. While the parasitemia of mice infected with Pemalang isolate increased in the blood (108-109/mL of blood) on day 4 and maintained for a few more days and then fluctuated for a few more days before the animal died.Key words: Trypanosoma evansi, parasitemia, Pidie isolate, Pemalang isolate

scholarly journals Cholinesterase as inflammatory markers in a experimental infection by Trypanosoma evansi in rabbits

2012 ◽  
Vol 84 (4) ◽  
pp. 1105-1113 ◽  
Author(s):  
Márcio M. Costa ◽  
Aleksandro S. da Silva ◽  
Francine C. Paim ◽  
Raqueli França ◽  
Guilherme L. Dornelles ◽  
...  
Keyword(s):  
Inflammatory Markers ◽  
Inflammatory Marker ◽  
Trypanosoma Evansi ◽  
Infected Group ◽  
Control Group ◽  
Post Inoculation ◽  
Animal Blood ◽  
Significant Difference ◽  
Encephalic Structures

The aim of this study is to evaluate the role of cholinesterases as an inflammatory marker in acute and chronic infection by Trypanosoma evansi in rabbits experimentally infected. Twelve adult female New Zealand rabbits were used and divided into two groups with 6 animals each: control group (rabbits 1-6) and infected group (rabbits 7-12). Infected group received intraperitoneally 0.5 mL of blood from a rat containing 108 parasites per animal. Blood samples used for cholinesterases evaluation were collected on days 0, 2, 7, 12, 27, 42, 57, 87, 102 and 118 days post-inoculation (PI). Increased activity (P<0.05) of butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) were observed in the blood on days 7 and 27, respectively and no differences were observed in cholinesterase activity in other periods. No significant difference in AChE activity (P>0.05) was observed in the encephalic structures. The increased activities of AChE and BChE probably have a pro-inflammatory purpose, attempting to reduce the concentration of acetylcholine, a neurotransmitter which has an anti-inflammatory property. Therefore, cholinesterase may be inflammatory markers in infection with T. evansi in rabbits.

Description of Sarcocystis rauschorum sp. n. (Protozoa: Sarcocystidae) with experimental cyclic transmission between varying lemmings (Dicrostonyx richardsoni) and snowy owls (Nyctea scandiaca)

1984 ◽  
Vol 62 (2) ◽  
pp. 217-225 ◽  
Author(s):  
Richard J. Cawthorn ◽  
Alvin A. Gajadhar ◽  
Ronald J. Brooks
Keyword(s):  
Small Intestine ◽  
Mus Musculus ◽  
Rattus Norvegicus ◽  
Striated Muscle ◽  
Prepatent Period ◽  
House Mice ◽  
Post Inoculation ◽  
Intermediate Hosts ◽  
Patent Period ◽  
Snowy Owl

Sarcocystis rauschorum sp. n. (Protozoa: Sarcocystidae) is heteroxenous, with varying lemmings (Dicrostonyx richardsoni) as expermental intermediate hosts and snowy owls (Nyctea scandiaca) as natural and experimental definitive hosts. Free sporocysts of a Sarcocystis-like organism (11.6 × 9.2 μm with a diffuse residuum of numerous small refractile granules) from intestinal scrapings of a naturally infected snowy owl were orally administered to laboratory-reared varying lemmings. Meronts were present 7 days post inoculation (DPI) in hepatocytes. Cysts of S. rauschorum sp. n. developed in striated muscle and contained metrocytes (4.6 × 3.8 μm) 14 DPI; bradyzoites (6 × 2 μm) were present at least as early as 28 DPI. Cysts at 84 DPI were not macroscopic (greatest diameter 80.9 μm, least diameter 70.9 μm) with walls 0.35–0.70 μm thick. Laboratory-reared rats (Rattus norvegicus), house mice (Mus musculus), white-footed mice (Peromyscus leucopus), red-backed voles (Clethrionomys gapped), and brown lemmings (Lemmus sibiricus) were not suitable intermediate hosts. Carcasses of varying lemmings containing cysts (57 and 84 DPI) of S. rauschorum sp. n. were fed to three Sarcocystis-free snowy owls. The prepatent period was 7 days and the patent period 12 – 19 days. Sporogony occurred in the lamina propria throughout the length of the small intestine. Free sporocysts (10.4 × 8.2 μm with a compact residuum of numerous small refractile granules) from experimentally infected owls were infective for varying lemmings.

scholarly journals Kerusakan Usus pada Mencit (Mus musculus) yang Diinokulasi Larva 3 (L3) Anisakis spp.

2017 ◽  
Vol 35 (1) ◽  
pp. 57
Author(s):  
Febrina Dian Permatasari ◽  
Poedji Hastutiek ◽  
Lucia Tri Suwanti
Keyword(s):  
Intestinal Mucosa ◽  
Mus Musculus ◽  
Structural Changes ◽  
Inflammatory Cells ◽  
Control Group ◽  
Post Inoculation ◽  
Male Mice ◽  
Histological Changes ◽  
Group A ◽  
Anisakis Spp

This study aims to be show damage in the intestine of mice caused by inoculation of the larvae of Anisakis spp. alive and who has died because of a warming 750C for 7 minutes. Eighteen male mice (Mus musculus) were divided into three groups: control group, a group of mice were inoculated the larvae of Anisakis spp. alive andgroups of mice were inoculated the larvae of Anisakis spp. who have died. 48 hours post inoculation, the mice’s intestines necropsy performed later performed HE staining to identify and scoring intestinal histopathology. The results showed inoculation of the larvae of Anisakis spp. either alive or dead induce histological changes in the intestine in the form of infiltration of inflammatory cells, epithelial changes and structural changes in the intestinal mucosa

scholarly journals Efek Inokulasi Trypanosoma Evansi Terhadap Histopatologi Jaringan Syaraf Mencit (Mus Musculus)

2018 ◽  
Vol 6 ◽  
Author(s):  
Epry Setiawan
Keyword(s):  
Mus Musculus ◽  
Trypanosoma Evansi ◽  
Test Method ◽  
Nerve Tissue ◽  
Control Group ◽  
Statistical Program ◽  
Group 2 ◽  
The Brain ◽  
Experimental Group ◽  
Group 1

The alms of this was analized research aims to analyze the damage of nerve tissue in the brain of mice (Mus Musculus) inoculated with Trypanosoma evansi. The animal used in this research was 27 male mice. The plan used in this research was the random complete plan (RAL) with one control group and two experimental groups used nine repetitions. Control group (PO) was given aquades, P1 group was inoculated with Trypanosoma evensi 0,2x104 ml by intraperitoneal, and then P2 group was inoculated with Trypanosma evansi 0,2x104 ml by subcutan. In the fourth day after inoculated the mice were sacrificed and taken the brain organ to make histopathology blood smear. The lesi result was analyzed by using scoring Kennedy method, it showed the result, there was a change of histopathology of nerve tissue in the brain organ of mice (Mus Musculus), it was lesion meningitis 33% was in the control group, 100% was in the experimental group 1 (P1), 100% was in the experimental group 2 (P2). Perivascullar cuffing was 0% in the control group, 100% was in the experimental group 1, and 88% was in the experimental group 2. The gained data was processed by using Statistical Program For Science (SPSS) with Krusskall-Wallis Test method and then with Mann-Whitney Test method. The result showed the real differences (P≤0,01) between control group and experimental group 2.

scholarly journals INOKULASI Trypanosoma evansi PADA MENCIT Mus musculus STRAIN BALB-C YANG BERASAL DARI DARAH SAPI LOKAL

2013 ◽  
Vol 7 (2) ◽  
Author(s):  
Yudha Fahrimal ◽  
Mecky Desca Saad ◽  
Hamdani Budiman
Keyword(s):  
Mus Musculus ◽  
Trypanosoma Evansi ◽  
Banda Aceh

Penelitian ini bertujuan mengetahui keberhasilan inokulasi Trypanosoma evansi (T. evansi) pada mencit Mus musculus strain Balb-C yang berasal dari darah sapi lokal di Rumah Potong Hewan kota Banda Aceh. Dari 205 sampel darah sapi lokal, 15 sampel positif mengandung T.evansi dengan metode hematokrit dan semua darah disuntikkan masing-masing pada  mencit secara intraperitoneal untuk memperbanyak jumlah parasit. Semua mencit diperiksa secara regular 2 kali seminggu dengan mengambil darah dari ujung ekor. Hasil penelitian menunjukkan bahwa dari 15 mencit yang diinokulasi, satu ekor mati pada minggu ke-3, satu ekor mati pada minggu ke-5, satu ekor mati minggu ke-8, dua ekor mati minggu ke-9, dan tiga ekor mati pada minggu ke-14, sementara 7 ekor masih hidup sampai minggu ke-20. Dari ke-8 ekor yang mati semuanya tidak ditemukan berkembangnya T. evansi pada mencit

Basal Lamina Formation in DMBA Induced Mammary Carcinoma

1977 ◽  
Vol 35 ◽  
pp. 534-535
Author(s):  
I. Russo ◽  
J. Saby ◽  
J. Russo
Keyword(s):  
Mammary Carcinoma ◽  
Virgin Female ◽  
Mammary Glands ◽  
Sesame Oil ◽  
Female Rats ◽  
Ultrastructural Changes ◽  
Post Inoculation ◽  
Intermediate Type ◽  
Sprague Dawley ◽  
Intercellular Spaces

It has been previously demonstrated that DMBA-induced rat mammary carcinoma originates in the terminal end bud (TEB) of the mammary gland by proliferation of intermediate type cells (1). The earliest lesion identified is the intraductal proliferation (IDP), which gives rise to intraductal carcinomas. These evolve to cribriform, papillary and comedo types (2). In the present work, we report the ultrastructural changes that take place in the IDP for the formation of a cribriform pattern.Fifty-five-day-old Sprague Dawley virgin female rats were inoculated intra- gastrically with 20 mg 7,12-dimethylbenz(a)anthracene (DMBA) in 1 ml sesame oil. Non-inoculated, age-matched females were used as controls. Mammary glands from both control and experimental rats were removed weekly from the time of inoculation until 86 days post-inoculation. The glands were fixed and processed for electron microscopy (2).The first change observed in IDP's was the widening of intercellular spaces and the secretion of an electron dense material into these spaces (Fig. 1).

Ultrastructural Changes in the Mammary Epithelial Cell Population During Neoplastic Development Induced by A Chemical Carcinogen.

1976 ◽  
Vol 34 ◽  
pp. 250-251 ◽  
Author(s):  
J. Russo ◽  
W. Isenberg ◽  
M. Ireland ◽  
I.H. Russo
Keyword(s):  
Mammary Gland ◽  
Cell Population ◽  
Virgin Female ◽  
Histological Change ◽  
Mammary Epithelial ◽  
Female Rats ◽  
Ultrastructural Changes ◽  
Post Inoculation ◽  
Chemical Carcinogen ◽  
Sprague Dawley

The induction of rat mammary carcinoma by the chemical carcinogen DMBA is used as a model for the study of the human disease (1). We previously described the histochemical changes that occur in the mammary gland of DMBA treated animals before the earliest manifested histological change, the intraductal proliferation (IDP), was observed (2). In the present work, we demonstrate that a change in the stable cell population found in the resting mammary gland occurs after carcinogen administration.Fifty-five day old Sprague-Dawley virgin female rats were inoculated intragastrically with 20mg of 7,12-dimethylbenz(a)anthracene (DMBA) in 1ml sesame oil. Non-inoculated, age-matched females were used as controls. Mammary glands from control and inoculated rats were removed weekly from the time of inoculation until 60 days post-inoculation. For electron microscopy, the glands were immersed in Karnovsky's fixative, post-fixed in 1% OsO4, dehydrated, and embedded in an Epon-Araldite mixture. Thick (lμ) sections were stained with 1% toluidine blue and were used for selecting areas for ultrastructural study.

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