scholarly journals Distribution and Antifungal Sensitivity of Candida Isolates Detected in Blood Cultures

ANKEM Dergisi ◽  
2020 ◽  
Author(s):  
Duygu Beder ◽  
Fatma Esenkaya Taşbent ◽  
Metin Doğan
Keyword(s):  
Intensive Care ◽  
Candida Species ◽  
Antifungal Susceptibility ◽  
Rapid Identification ◽  
Blood Cultures ◽  
Automated System ◽  
Candida Spp ◽  
Intensive Care Patients ◽  
Gram Staining

Candidemia is a serious clinical condition that can result in mortality. This condition is particularly common in intensive care patients. This retrospective study aimed to identify Candida strains isolated from blood cultures and to determine antifungal susceptibility patterns. For this purpose, isolated Candida species were identified and, their susceptibility to amphotericin B, caspofungin, flucytosine, fluconazole, micafungin and voriconazole were investigated. VITEK 2 automated system was used to identify and detect antifungal susceptibility of Candida species that were subjected to Gram staining and germ tube tests on Candida species detected from blood cultures sent to our laboratory between January 2014 and December 2018. Candida spp. isolation rate was found most frequently (n=157; 64.9 %) in intensive care units; followed by internal clinics (n=64; 26.5 %) and surgical clinics (n=21; 8.6 %). The most frequently isolated species among these Candida isolates are Candida albicans (100/242; 41.3 %) and Candida parapsilosis (92/242; 38 %). Especially in intensive care patients, rapid identification of Candida species and determination of their antifungal susceptibilities are important for planning treatment. It is thought that collecting data reflecting regional resistance status at certain intervals will be guiding in terms of treatment approaches.

scholarly journals Analysis of Сandida species distribution isolated from women with vulvovaginal candidiasis and their antifungal susceptibility profile

2021 ◽  
Vol 9 (6) ◽  
pp. 225-229
Author(s):  
O.S. Burduniuc ◽  
E.I. Bîrcă ◽  
A.M. Burduniuc ◽  
M.V. Bivol ◽  
O.S. Iacon
Keyword(s):  
Species Distribution ◽  
Candida Species ◽  
Antifungal Susceptibility ◽  
Common Species ◽  
Resistance Rate ◽  
Vulvovaginal Candidiasis ◽  
Automated System ◽  
Clinical Samples ◽  
Bacterial Strains ◽  
Candida Spp

Background. Candida species is usually a commensal fungus residing in the vagina, in about 30 %–50 % of healthy adults and remains the most common agent isolated from clinical samples of patients diagnosed with vulvovaginal candidiasis (VVC). Culture is not recommended for all patients on a routine basis but is informative for the exclusion of non-albicans infections resistant to azoles, or recurrent VVC. Identification of Candida species and antifungal susceptibility testing are key elements in the correct management of recurrent VVC. The authors of this paper aimed to study the spectrum of pathogenic Candida species. The antifungal sensitivity patterns of the isolated strains during the years 2017-2019 are also presented. Material and methods. A descriptive study of Candida species distribution and antifungal susceptibility profile was performed. Identification of isolated Candida species was performed by the MALDI-TOF MS, bacterial strains by Vitek 2 automated system (BioMérieux) and antifungal susceptibility profiles by Fungitest (Bio-Rad). Results. Of the 1030 recovered strains, C. albicans species predominated - 83.8% and Candida non albicans -16.2%. The most common species of the Candida non-albicans, were C.glabrata (7.5%). Also, it was observed that in 56.6% of the cases C. albicans presented bacterial associations. The most common microbial association was C. albicans and S.aureus (26.6%), followed by C. albicans and E.coli (20.8%). Candida spp. showed moderate antifungal resistance. The resistance rate of C. albicans isolates to miconazole, fluconazole and itraconazole was 25.5%, 17.0% and 11.4% compared to non-albicans Candida strains which showed a resistance rate of 10,8%, 14.4% and 10%, respectively. Conclusions. C. albicans is the most frequently isolated species among patients with VVC, but there is also an increase share of non-albicans Candida species such as C. glabrata. C. albicans isolates showed higher indices of resistance, than Candida non-albicans.

scholarly journals Sensitivity to antifungals by Candida spp samples isolated from cases of chronic atrophic candidiasis (CAC)

2020 ◽  
Vol 80 (2) ◽  
pp. 266-272
Author(s):  
L. C. Reinhardt ◽  
P. S. Nascente ◽  
J. S. Ribeiro ◽  
V. B. S. Guimarães ◽  
A. Etges ◽  
...  
Keyword(s):  
Antifungal Agents ◽  
Antifungal Susceptibility ◽  
Automated System ◽  
Candida Spp ◽  
Chi Square ◽  
Denture Stomatitis ◽  
Fungal Identification ◽  
Chi Square Test ◽  
Prevalent Species

Abstract The treatment of choice for chronic atrophic candidiasis (CAC), also known as denture stomatitis, is topical antifungal therapy. This study aimed to isolate, identify, and assess the antifungal susceptibility of Candida species from mucosal sites in denture wearers with a diagnosis of CAC and determine the prevalence of associated variables. The sample consisted of 44 patients wearing complete or partial dentures who had a clinical diagnosis of CAC. Using sterile cotton swabs, specimens were collected from the oral mucosa of all patients and grown at 30ºC for 48 h in CHROMagar Candida, as a means of isolating and screening the species. The complementary identification of the species was performed using the VITEK 2 automated system (BioMérieux), as well as the determination of their susceptibility to antifungal agents. Data were analyzed using the chi-square test. STATA 13.1 was used for statistical analysis (α = 5%). Of 44 patients with CAC, 33 (75%) had lesions classified as Newton type II. Yeasts were isolated in 38 cases. The most prevalent species was Candida albicans. None of the isolates were resistant to the antifungals tested. Our findings suggest that current indications for antifungal agents are appropriate. Also, antifungal susceptibility testing and proper fungal identification can help dentists to determine the optimal course of treatment for CAC.

scholarly journals Prevalence and antifungal drug resistance of nosocomial Candida species isolated from two university hospitals in Egypt

2021 ◽  
Author(s):  
Amira M. El-Ganiny ◽  
Nehal E. Yossef ◽  
Hend A. Kamel
Keyword(s):  
Amphotericin B ◽  
Candida Species ◽  
Antifungal Susceptibility ◽  
Rapid Identification ◽  
Significant Rise ◽  
Diffusion Method ◽  
University Hospitals ◽  
Candida Spp ◽  
Antifungal Drug Resistance ◽  
Resistance Rates

Background and Purpose: There is a significant rise in morbidity and mortality of infections caused by Candida. Candida spp. infections are currently ranked fourth among nosocomial infections which are difficult to diagnose and refractory to therapy.Given the differences in susceptibility among various spp., identification of Candida spp. is an important step that leads to the selection of a suitable antifungal. Materials and Methods: A prevalence study was on 122 Candida isolates. The Candida spp. were identified using Chromogenic agar and polymerase chain reaction (PCR). The antifungal susceptibility (AFS) of Candida spp. to amphotericin B, fluconazole,voriconazole, and caspofungin was determined by the disc diffusion method. Results: In total, 122 Candida clinical isolates were investigated in this study. Candida albicans with 57.4% (70 isolates) had the highest prevalence rate, while 52 isolates (42.6%) were non-albicans Candida species (NAC). The NAC include Candida krusei (20.4%), Candida tropicalis (6.5%), Candida parapsilolsis (5.7%), Candida dubliniensis (4.9%), and Candida glabrata (4.9%). The AFS showed that the resistance rates of Candida spp. to fluconazole and voriconazole were 13.1% (16 isolates) and 9.8% (12 isolates), respectively. Moreover, only five isolates (4.1%) were resistant to caspofungin. Furthermore, there was no resistance against amphotericin B. The spp. that showed the highest resistance were C. glabrata and C. tropicalis, while the lowest resistance was observed in C. albicans and C. dubliniensis. Conclusion: In conclusion, rapid identification of clinical Candida isolates and standard AFS are essential procedures for controlling the rise of resistant NAC spp.in clinical settings. Usage of fluconazole should be restricted, especially in patients with recurrent Candida infections.

Distribution and antifungal susceptibility of candida species isolated from blood culture

2021 ◽  
pp. 1-12
Author(s):  
Selim Gorgun ◽  
Melek Bilgin ◽  
Suleyman Sirri Kilic ◽  
Admin
Keyword(s):  
Candida Species ◽  
Antifungal Susceptibility ◽  
Tube Formation ◽  
Resistance Rate ◽  
Blood Cultures ◽  
Dilution Method ◽  
Ethical Approval ◽  
Immunosuppressed Patients ◽  
Training And Research ◽  
Vitek 2

Abstract Objective: The aim of this study is to determine the distribution of samples exhibiting Candida growth among the blood cultures, and the antifungal susceptibility. Methods: The retrospective study was conducted in Samsun Training and Research Hospital, Samsun, Turkey from January to December, 2018 and comprised immunosuppressed patients with sepsis. Ethical approval was obtained and consent was taken from all patients. Yeast growths were typed by means of colony morphology, germ tube formation and the VITEK 2 system. Their susceptibilities tests were determined using the same automatized system. Resistance strains were also tested using the dilution method. Results: Of the 50 patients, 19(38%) were females and 31(62%) were males. The overall mean age was 60.84 ± 22.05 years. 31 (62%) of the samples were received from intensive care units, 14 (28%) from the palliative care unit, and five from other inpatients.  In our study, most common among our isolates was C. albicans, and C. parapsiosis was most common among non-albicans species. Resistance to antifungals was observed in 28% of Candida isolates. Of the total isolates, %10 were found to be naturally resistant to fluconazole, 8% to voriconazole, 4% to flucytosine, and amphotericin B. The high resistance rate for fluconazole in Candida species is noteworthy. The findings obtained through both the dilution method and the automatized system were consistent. Continuou....

The yeast, the antifungal, and the wardrobe: a journey into antifungal resistance mechanisms of Candida tropicalis

2020 ◽  
Vol 66 (6) ◽  
pp. 377-388
Author(s):  
Jonathas Sales de Oliveira ◽  
Vandbergue Santos Pereira ◽  
Débora de Souza Collares Maia Castelo-Branco ◽  
Rossana de Aguiar Cordeiro ◽  
José Júlio Costa Sidrim ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Intensive Care ◽  
Candida Tropicalis ◽  
Clinical Management ◽  
Candida Species ◽  
Genetic Basis ◽  
Antifungal Susceptibility ◽  
Resistance Mechanisms ◽  
Antifungal Resistance ◽  
Resistant Strains

Candida tropicalis is a prominent non-Candida albicans Candida species involved in cases of candidemia, mainly causing infections in patients in intensive care units and (or) those presenting neutropenia. In recent years, several studies have reported an increase in the recovery rates of azole-resistant C. tropicalis isolates. Understanding C. tropicalis resistance is of great importance, since resistant strains are implicated in persistent or recurrent and breakthrough infections. In this review, we address the main mechanisms underlying C. tropicalis resistance to the major antifungal classes used to treat candidiasis. The main genetic basis involved in C. tropicalis antifungal resistance is discussed. A better understanding of the epidemiology of resistant strains and the mechanisms involved in C. tropicalis resistance can help improve diagnosis and assessment of the antifungal susceptibility of this Candida species to improve clinical management.

scholarly journals Candida Species and Antifungal Susceptibility Isolated in Intensive Care Units: A Three-year Study

2021 ◽  
Vol 28 (3) ◽  
pp. 459-465
Author(s):  
Sule Batcık ◽  
İlkay Bahçeci ◽  
Leyla Kazancioglu ◽  
Hizir Kazdal ◽  
Mustafa Özcan
Keyword(s):  
Intensive Care ◽  
Intensive Care Units ◽  
Candida Species ◽  
Antifungal Susceptibility

scholarly journals 680. Month Long Fungemia due to Candida auris Endocarditis

2021 ◽  
Vol 8 (Supplement_1) ◽  
pp. S443-S443
Author(s):  
Haseeba khan ◽  
Christy Varughese ◽  
Hemil Gonzalez
Keyword(s):  
Fungal Infections ◽  
Antifungal Susceptibility ◽  
Acute Care Hospital ◽  
Sigmoid Resection ◽  
Blood Cultures ◽  
Multi Drug Resistance ◽  
Care Hospital ◽  
Candida Auris ◽  
Candida Spp ◽  
First Case

Abstract Background Candida auris (C. auris) is a multidrug resistant Candida species, reported to cause persistent fungemia along with a multitude of invasive fungal infections. We report the first case of C. auris fungemia due to endocarditis. Methods 61 year old man with a history of diverticulitis that required sigmoid resection and was complicated by abdominal abscesses due to multi drug resistant organisms warranting heavy antibiosis. Prolonged hospitalisation for that surgery was followed by a stay at a long term acute care hospital. He was readmitted at an outside hospital with sepsis where blood cultures grew C.auris. Upon evaluation, was found to have aortic valve endocarditis. Per patient’s preference, surgery was initially deferred. Despite escalation of therapy with a combination of antifungals, he remained fungemic for five weeks with repeat blood cultures showing changing antifungal susceptibility patterns. Patient eventually underwent surgical intervention at our facility, with valve cultures being positive for C.auris. After the surgery he was treated with 6 weeks of intravenous combination antifungal therapy. Results C.auris’s pathogenicity stems from multiple mechanisms with multi drug resistance being most pertinent. What adds to the complexity of the management is the absence of C.auris specific minimum inhibitory concentration breakpoints. Therefore treatment is based on Center for Disease Control’s (CDC) proposed breakpoints that have been extrapolated from other Candida spp. It is further complicated by lack of C.auris specific data showing essential agreement among different commercially available antifungal susceptibility testing (AFST). Heteroresistance of the microbial population is an issue that must be considered in such protracted fungemia. Conclusion Invasive infections due to Candida auris presents as a diagnostic and therapeutic challenge to clinicians. Disclosures All Authors: No reported disclosures

Sign in / Sign up

Export Citation Format

Share Document