scholarly journals The Relationship Between the Use of Hydrogel and Silicone Hydrogel Contact Lenses and Coagulase-Negative Staphylococci Population in the Conjunctiva and Biofilm Forming Staphylococcus epidermidis

2021 ◽  
Author(s):  
Zeynep Güngördü Dalar ◽  
Güzin İskeleli ◽  
Mert Ahmet Kuşkucu ◽  
Mehmet Demirci ◽  
Penbe Çağatay ◽  
...  
Keyword(s):  
Contact Lens ◽  
Staphylococcus Epidermidis ◽  
Congo Red ◽  
Molecular Methods ◽  
Coagulase Negative Staphylococci ◽  
Silicone Hydrogel ◽  
Slime Production ◽  
Microbiological Methods ◽  
Cutibacterium Acnes ◽  
Before And After

Objective: The most important bacteria of the conjunctival microbiota are Staphylococcus epidermidis, diphteroid rods, Corynebacterium spp. and Cutibacterium acnes. Especially biofilm formation of S. epidermidis is very important for contact lens related infections. For this purpose, we aimed to examine the changes in the presence of biofilm-forming S. epidermidis and other coagulase-negative staphylococci in conjunctival swabs taken before and after lens usage in 140 patients (90 hydrogel, 50 silicone hydrogel) who were prepared to wear lenses. Methods: Coagulase-negative staphylococci isolated from the conjunctival microbiota identified standard clinical microbiological methods, after identification of S.epidermidis strains with API Staph; Slime production was determined by Congo red agar, standard tube and molecular methods. Results: S.epidermidis was the most frequently isolated species in conjunctival microbiota before and after lens usage. Before lens usage, slime positive S. epidermidis strains were found as 45-50% but after lens usage it was 59% in hydrogel contact lens users and 70.2% in silicone hydrogel contact lens users. For the investigation of slime production, 82 (50.9%) of 161 S. epidermidis strains were found positive by using Congo red agar, 61 (37.8%) by standard tube method and 91 (56.5%) by molecular methods. Conclusion: The result of our study suggests that there are no significant changes in bacterial ratios before and after lens use, but bacteria such as S. epidermidis can predispose to infections by using slime production and contact lens factor. Also; molecular methods and Congo Red Agar method were found to be more reliable than the Standard Tube method.

scholarly journals Species identification, antibiotic sensitivity and slime production of coagulase-negative staphylococci isolated from clinical specimens

1988 ◽  
Vol 101 (1) ◽  
pp. 99-113 ◽  
Author(s):  
M. A. Deighton ◽  
J. C. Franklin ◽  
W. J. Spicer ◽  
B. Balkau
Keyword(s):  
Antibiotic Resistance ◽  
Staphylococcus Epidermidis ◽  
Clinical Relevance ◽  
Antibiotic Sensitivity ◽  
Hospital Environment ◽  
Multiple Antibiotic Resistance ◽  
Coagulase Negative Staphylococci ◽  
Clinical Specimens ◽  
Slime Production ◽  
Common Skin

SUMMARYTwo hundred and seventy-five consecutive clinical isolates of coagulase-negative staphylococci, including strains associated with disease, contaminants and skin colonizers, were speciated, tested for slime production and for their sensitivity to a range of antibiotics.Staphylococcus epidermidiswas the most commonly identified species, comprising 63% of all isolates. Slime production was detected in half the strains ofStaph. epidermidis, Staph. haemolyticusandStaph. Saprophyticusbut was rare in other species. MostStaph. haemolyticusstrains and approximately half of theStaph. epidermidisstrains were resistant to five or more antibiotics. A significant association was found between slime production and multiple antibiotic resistance. For catheter-associated strains, clinical relevance was predictable by species i.e.Staph. epidermidis. Multi-resistant slime-positiveStaph. haemolyticusstrains, although infrequently associated with disease, were common skin colonizers, presumably acquired from the hospital environment. We describe a practical and inexpensive scheme for thespeciation of human coagulase-negative staphylococcal isolates.

scholarly journals Study of Myopia Correction Using Nanoporous Polymer Gas Permeable Contact Lenses

2022 ◽  
Vol 58 (4) ◽  
pp. 55-64
Author(s):  
Silvia Salavastru ◽  
Irina Andreea Niagu ◽  
Luana Andrei Macovei ◽  
Lucretia Anghel ◽  
Nicolae Sarbu ◽  
...  
Keyword(s):  
Contact Lens ◽  
Contact Lenses ◽  
Anterior Segment ◽  
Silicone Hydrogel ◽  
Before And After ◽  
Materials Used ◽  
Ocular Comfort ◽  
Myopic Astigmatism ◽  
Lens Wear

Ophthalmic treatments using contact lenses are now used by more and more specialists around the world. Improving contact lens (CL) materials is a condition that is constantly evolving. Contact lens materials are usually composed of polymer hydrogel or silicone hydrogel. The materials used for night contact lenses are gas permeable and they gradual flatten the center of the cornea which decreases the progression of myopia or myopic astigmatism. The aim of this study is to identify, in correlation with the chemical interactions between structural components of contact lenses and their biocompatibility with the surface layer and microtopography of the cornea or sclera, different incidents that occur in patients who have used rigid gas permeable contact lenses. The study was performed on a group of 10 patients who had a follow-up period between 4 and 6 years, who presented regularly for eye examinations. The following clinical parameters were analyzed: ocular refraction before and after wearing night contact lenses, types of contact lens materials, appearance of corneal topography, biomicroscopic examination of the anterior segment of the eye, keratometry, ocular comfort, as well as other incidents regarding this type of lenses. Difficulties caused by wearing contact lenses at night arose due to poor hygiene and maintenance in two cases or due to interruption of lens wear in one case.

Detection of slime production by means of an optimised Congo red agar plate test based on a colourimetric scale in Staphylococcus epidermidis clinical isolates genotyped for ica locus

Biomaterials ◽  
2002 ◽  
Vol 23 (21) ◽  
pp. 4233-4239 ◽  
Author(s):  
Carla Renata Arciola ◽  
Davide Campoccia ◽  
Simonetta Gamberini ◽  
Marina Cervellati ◽  
Elena Donati ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Congo Red ◽  
Agar Plate ◽  
Clinical Isolates ◽  
Plate Test ◽  
Slime Production

scholarly journals Synthesis, Characterization and Testing of Antimicrobial Activity of Composites of Unsaturated Polyester Resins with Wood Flour and Silver Nanoparticles

Materials ◽  
2021 ◽  
Vol 14 (5) ◽  
pp. 1122
Author(s):  
Przemysław Pączkowski ◽  
Andrzej Puszka ◽  
Malgorzata Miazga-Karska ◽  
Grażyna Ginalska ◽  
Barbara Gawdzik
Keyword(s):  
Silver Nanoparticles ◽  
Staphylococcus Epidermidis ◽  
Polyester Resin ◽  
Wood Flour ◽  
Unsaturated Polyester ◽  
Thermomechanical Properties ◽  
Resin Composites ◽  
Polyester Resins ◽  
Before And After

This paper presents the properties of the wood-resin composites. For improving their antibacterial character, silver nanoparticles were incorporated into their structures. The properties of the obtained materials were analyzed in vitro for their anti-biofilm potency in contact with aerobic Gram-positive Staphylococcus aureus and Staphylococcus epidermidis; and aerobic Gram-negative Escherichia coli and Pseudomonas aeruginosa. These pathogens are responsible for various infections, including those associated with healthcare. The effect of silver nanoparticles incorporation on mechanical and thermomechanical properties as well as gloss were investigated for the samples of composites before and after accelerating aging tests. The results show that bacteria can colonize in various wrinkles and cracks on the composites with wood flour but also the surface of the cross-linked unsaturated polyester resin. The addition of nanosilver causes the death of bacteria. It also positively influences mechanical and thermomechanical properties as well as gloss of the resin.

scholarly journals Prevalence of Antimicrobial-Resistant Pathogens in Canadian Hospitals: Results of the Canadian Ward Surveillance Study (CANWARD 2008)

2010 ◽  
Vol 54 (11) ◽  
pp. 4684-4693 ◽  
Author(s):  
George G. Zhanel ◽  
Melanie DeCorby ◽  
Heather Adam ◽  
Michael R. Mulvey ◽  
Melissa McCracken ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Susceptibility Testing ◽  
Multidrug Resistant ◽  
Surveillance Study ◽  
Bacterial Isolates ◽  
Coagulase Negative Staphylococci ◽  
Clinical Specimens ◽  
E Coli ◽  
Polymyxin E ◽  
Resistance Rates

ABSTRACT A total of 5,282 bacterial isolates obtained between 1 January and 31 December 31 2008, inclusive, from patients in 10 hospitals across Canada as part of the Canadian Ward Surveillance Study (CANWARD 2008) underwent susceptibility testing. The 10 most common organisms, representing 78.8% of all clinical specimens, were as follows: Escherichia coli (21.4%), methicillin-susceptible Staphylococcus aureus (MSSA; 13.9%), Streptococcus pneumoniae (10.3%), Pseudomonas aeruginosa (7.1%), Klebsiella pneumoniae (6.0%), coagulase-negative staphylococci/Staphylococcus epidermidis (5.4%), methicillin-resistant S. aureus (MRSA; 5.1%), Haemophilus influenzae (4.1%), Enterococcus spp. (3.3%), Enterobacter cloacae (2.2%). MRSA comprised 27.0% (272/1,007) of all S. aureus isolates (genotypically, 68.8% of MRSA were health care associated [HA-MRSA] and 27.6% were community associated [CA-MRSA]). Extended-spectrum β-lactamase (ESBL)-producing E. coli occurred in 4.9% of E. coli isolates. The CTX-M type was the predominant ESBL, with CTX-M-15 the most prevalent genotype. MRSA demonstrated no resistance to ceftobiprole, daptomycin, linezolid, telavancin, tigecycline, or vancomycin (0.4% intermediate intermediate resistance). E. coli demonstrated no resistance to ertapenem, meropenem, or tigecycline. Resistance rates with P. aeruginosa were as follows: colistin (polymyxin E), 0.8%; amikacin, 3.5%; cefepime, 7.2%; gentamicin, 12.3%; fluoroquinolones, 19.0 to 24.1%; meropenem, 5.6%; piperacillin-tazobactam, 8.0%. A multidrug-resistant (MDR) phenotype occurred frequently in P. aeruginosa (5.9%) but uncommonly in E. coli (1.2%) and K. pneumoniae (0.9%). In conclusion, E. coli, S. aureus (MSSA and MRSA), P. aeruginosa, S. pneumoniae, K. pneumoniae, H. influenzae, and Enterococcus spp. are the most common isolates recovered from clinical specimens in Canadian hospitals. The prevalence of MRSA was 27.0% (of which genotypically 27.6% were CA-MRSA), while ESBL-producing E. coli occurred in 4.9% of isolates. An MDR phenotype was common in P. aeruginosa.

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