scholarly journals ANTIOXIDANT ACTIVITIES OF HYPERICUM FURSEI L.

2014 ◽  
Vol 44 (1) ◽  
pp. 19-23
Author(s):  
B. S. ALIPOUR ◽  
M. A. EBRAHIMZADEH ◽  
B. ESLAMI ◽  
Z. RAHMANI
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Chelating Ability ◽  
Dpph Radical Scavenging ◽  
Gallic Acid Equivalent

In this study, antioxidant activities of H. fursei were investigated. Different model were used for assay. IC50 for DPPH radical-scavenging activity was 7.5±1.2 μg ml-1. The IC50 value for BHA was 92.9±4.5 µg ml-1. The extract showed very good reducing power that was comparable with vitamin C (p> 0.05) but showed weak nitric oxide scavenging activity and Fe2+ chelating ability. Extract showed good activity in scavenging of H2O2. Inhibition was 99.1% at 800 μg ml-1. The IC50 values for extract and BHA were 333.1±14.7 and 52.0±4.5 μg ml-1, respectively. Total phenol compounds, by the Folin Ciocalteu method, was 274±9.6 mg gallic acid equivalent/g of extract and the total flavonoid content, by AlCl3 method was 23.9 ± 1.4 mg quercetin equivalent/g of extract. Antioxidant activity may be attributed to the presence of phenols and flavonoids in the extract.

In Vitro Free Radical Scavenging Activity and Total Flavonoid of Abelmoschus manihot (L.) Medic Extracts

2011 ◽  
Vol 140 ◽  
pp. 355-359 ◽  
Author(s):  
Yan Qiu ◽  
Jian Jun Song
Keyword(s):  
Ascorbic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Flavonoid ◽  
Scavenging Activity ◽  
Ic50 Values ◽  
Hydrogen Radicals ◽  
Dpph Radical Scavenging

The Ablmoschus manihot (L.) Medic, an edible hibiscus of the Malvaceae family, is abundant with total flavonoid. The content of total flavonoid from A. manihot flowers (TFA) was extracted by 70% ethanol (ethanol:water, 70:30). The scavenging effects of TFA on superoxide anions and hydrogen radicals were related to the total flavonoid concentrations with the IC50 values of 63.90 ±2.21 μg/mL and 266.88±28.32 μg/mL, respectively. However, the IC50 values of standard ascorbic acid were 436.52 ±14.36 μg/mL and 439.58±21.41 μg/mL, respectively. The DPPH radical scavenging activity increased with increasing of TFA concentrations and the highest inhibition was 94.63 ±3.01% at 50 μg/mL, as compared to ascorbic acid 34.94 ±1.50%. The extract also showed good reducing power. The data from this study suggest A. manihot flower extract has significant potential to use as health supplements and nutraceuticals.

Chemical Composition of Essential Oil and Antioxidant Activities of Curcuma petiolata Roxb. Rhizomes

2012 ◽  
Vol 506 ◽  
pp. 393-396 ◽  
Author(s):  
A. Thakam ◽  
N. Saewan
Keyword(s):  
Ascorbic Acid ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Etoac Extract ◽  
Dpph Radical Scavenging

Pulverized rhizome of Curcuma peliolata on hydrodistillation, afforded light yellow essential oil in the yield of 0.13% v/w. The chemical constituent of the essential oil was determined by GC-MS. Nine compounds representing 95.34 % of the total oil were identified. The major compounds of the oil were 2-methyl-5-pentanol, 1H-pyrrol-1-amine,2-(4-methoxyphenyl)-n,n,5-trimethyl, and curcumol. The distillated aqueous was extracted with dicloromethane (DCM), ethyl acetate (EtOAc), and n-butanol. The DPPH radical scavenging and reducing power of the extracts were investigated. Curcumin and ascorbic acid were used as standard references. The DPPH radical scavenging activity was decrease in order: curcumin ascorbic acid EtOAc extract > DCM extract > n-butanol extract > aqueous extract. The EtOAc extract showed significantly highest inhibition of the radical scavenging activity with IC50 0.092 ± 0.001 mg/mL (curcumin and ascorbic acid (IC50 0.012 and 0.013 mg/mL, respectively (P<0.05)). While, the DCM extract showed highest reducing power with IC50 = 0.271 mg/mL which lower than that of standard curcumin (IC50 = 0.013 mg/mL) and ascorbic acid (IC50 = 0.002 mg/mL).Introduction

scholarly journals Callus formation, phenolics content and related antioxidant activities in tissue culture of a medicinal plant colocynth (Citrullus colocynthis)

2021 ◽  
Vol 10 (2) ◽  
pp. 79-94
Author(s):  
Farouk K. El-Baz ◽  
Amal A. Mohamed ◽  
Sami I. Ali
Keyword(s):  
Total Phenolics ◽  
Antioxidant Activities ◽  
Callus Formation ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Callus Cultures ◽  
Scavenging Activity ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

Callus cultures from stems, leaves and roots of colocynth were initiated on MS media supplemented with various combinations of 2,4 dichlorophenoxyacetic acid (2,4-D) with kinetin (KIN) and benzyladenine (BA) with α-naphthaleneacetic acid (NAA). The highest percentage of callus formation frequency (98.9%) was obtained from stem explants grown on MS media supplemented with (1.0 mg/L) 2,4-D + (1.0 mg/L) KIN. The total phenolics and flavonoid content of the colocynth callus cultures were measured. The results showed that the MS medium supplemented with 6.0 mg/L 2,4-D + 2.0 mg/L KIN (MD3) gave the highest content of total phenolics (19.2 mg/100g d.w.) in leaf-derived calli. The highest content of flavonoids (47.3 mg/100g d.w.) was obtained in stem derived calli grown on the same medium (MD3). Antioxidant activities of extracts were determined using different assays, including DPPH radical scavenging activity, hydrogen peroxide (H2O2) scavenging activity and ferric reducing power. Leaf-derived calli cultured on MS medium + 2.0 mg/L 2,4-D + 1.0 mg/L KIN (MD1) showed the highest DPPH radical scavenging activity (85.3%). The highest percentage of H2O2 scavenging activity (61.4%) was detected in leaf explant-derived calli growing on MD1. The leaf-derived calli growing on (MD3) gave the highest ferric reducing power (22.3 μg/g d.w.), compared to the activities of stems, leaves and roots of in vitro grown seedlings (3.28, 12.9 and 2.85 μg/g d.w.), which were used as controls. On the basis of the current findings, we conclude that MS media supplemented with different combinations of 2,4-D and KIN yields higher phenolics, flavonoids contents and antioxidant activities than MS media supplemented with BA and NAA.

Antioxidant Potential and Bioactive Compounds in Extract and Fractions from the Fruit of Smilax china: an Underutilised Edible Berry

2013 ◽  
Vol 709 ◽  
pp. 883-886
Author(s):  
Hai Lan Huang ◽  
Hai Yuan Wang ◽  
Shu Mei Qiu ◽  
Zong Hua Wang
Keyword(s):  
Bioactive Compounds ◽  
Soluble Fraction ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Total Flavonoids Content ◽  
Dpph Radical Scavenging

The fruit of Smilax china is an edible spherical berry. The content of some bioactive compounds, antioxidant activities in three different assays, and the relationships between these bioactive compounds and antioxidant activity of the extracts derived from the peel of the fruit of were investigated. The results showed that the ethyl acetate-soluble fraction (EAF) was the richest fraction in total phenolic, total flavonoids content. The n-butanol-soluble fraction (BF) had the highest concentration of anthocyanins. EAF and BF showed good DPPH radical-scavenging activity (DRSA) and reducing power (RP), while the crude extract (CE) possessed better hydroxyl radical-scavenging activity (HRSA) than other fractions. There were statistically significant correlations between some antioxidants and antioxidant activities.

Antioxidant Activity and Total Phenolics of Broussonetia papyrifera Flower Extracts

2011 ◽  
Vol 140 ◽  
pp. 263-267 ◽  
Author(s):  
Jie Sun ◽  
Chu Shu Zhang ◽  
Li Na Yu ◽  
Jie Bi ◽  
Shao Fang Liu ◽  
...  
Keyword(s):  
Total Phenolics ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Broussonetia Papyrifera ◽  
Dpph Radical Scavenging

Ethanol and water crude extracts from the flower ofBroussonetia papyriferawere investigated for their antioxidant capacity in four different assays, namely, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, chelation of metal ions (Fe2+), reducing power and inhibition activity of lipid peroxidation in a linoleic acid emulsion system. Among the two extracts, the ethanolextract fromB. papyriferaflower showed the most potent radical scavenging activity in each assay, showing 62.88.9% (at 5 mg/ml) in the DPPH radical scavenging method (at 6 mg/ml) and 61.15% of chelation Fe2+- activity. Total phenolics in the water extracts were higher than that of the ethanol ones. Positive correlations were found between total phenolic content in theB. papyriferaflower extracts and their antioxidant activities.B. papyriferaflowers may have potential as natural antioxidants.

scholarly journals Antioxidant and scolicidal activities of four Iranian Mentha species (Lamiaceae) in relation to phenolic elements

2020 ◽  
Vol 9 (3) ◽  
pp. 200-208
Author(s):  
Mojtaba Ranjbar ◽  
Mahmoud Kiani ◽  
Ali Nikpay
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Mentha Spicata ◽  
Synthetic Chemicals ◽  
Reducing Power Assay ◽  
Dpph Radical Scavenging ◽  
Gallic Acid Equivalent

Introduction: There is a growing interest of industry to replace synthetic chemicals by natural products with bioactive properties from plant origin. The present study reported the antioxidant activity and scolicidal effects of the crude extracts from Mentha spicata, M. aquatica, M. longifolia, and M. × piperita var. citrata growing in Iran. Methods: Total phenolic, flavonoid and flavonol contents of the four Mentha taxa were examined. Two antioxidant assays i.e. free radical scavenging activity (DPPH assay) and reducing power assay were used for determining the antioxidant capacity of the alcoholic extracts. Scolicidal activity of serial dilutions (15–200 mg/mL) of Mentha extracts was evaluated after 1, 5, 10, 20 and 30 minutes of exposure time. Results: Methanol was the solvent yielding the highest values of total phenolic (TPC), flavonoid (TFC) and flavonol contents (TFvC). On the other hand, the extracts from M. × piperita var. citrata gave the highest total phenolic content (191.6 mg gallic acid equivalent per g; GAE/g), total flavonoid content (57.0 mg quercetin per g; QE/g) and total flavonol content (15.3 mg QE/g) values. The methanol extracts of M. × piperita var. citrata also gave the strongest DPPH radical scavenging activity (83.2%), whereas the reducing power yielded absorbance values between 0.189 in M. spicata and 1.16 in M. × piperita var. citrata. The highest scolicidal activity (99.54%) was observed in 200 mg/mL methanol extract of M. aquatica after 30 minutes of application. Conclusion: Overall, M. × piperita var. citrata and M. aquatica could be the taxa of choice for future supplementary studies.

scholarly journals Antiglycation and Antioxidant Properties of Ficus deltoidea Varieties

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Nur Sumirah Mohd Dom ◽  
Nurshieren Yahaya ◽  
Zainah Adam ◽  
Nik Mohd Afizan Nik Abd. Rahman ◽  
Muhajir Hamid
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Protein Carbonyl ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Ficus Deltoidea ◽  
Dpph Radical Scavenging

The present study aimed to evaluate the potential of standardized methanolic extracts from seven Ficus deltoidea varieties in inhibiting the formation of AGEs, protein oxidation, and their antioxidant effects. The antiglycation activity was analyzed based on the inhibition of AGEs, fructosamine, and thiol groups level followed by the inhibition of protein carbonyl formation. The antioxidant activity (DPPH radical scavenging activity and reducing power assay) and total phenolic contents were evaluated. After 28 days of induction, all varieties of Ficus deltoidea extracts significantly restrained the formation of fluorescence AGEs by 4.55–5.14 fold. The extracts also reduced the fructosamine levels by 47.0–86.5%, increased the thiol group levels by 64.3–83.7%, and inhibited the formation of protein carbonyl by 1.36–1.76 fold. DPPH radical scavenging activity showed an IC50 value of 66.81–288.04 μg/ml and reducing power activity depicted at 0.02–0.24 μg/ml. The extent of phenolic compounds present in the extracts ranged from 70.90 to 299.78 mg·GAE/g. Apart from that, correlation studies between the activities were observed. This study revealed that seven varieties of Ficus deltoidea have the potential to inhibit AGEs formation and possess antioxidant activity that might be attributed to the presence of phenolic compounds.

scholarly journals Antioxidant and Anticancer Activities of Wampee (Clausena lansium(Lour.) Skeels) Peel

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
K. Nagendra Prasad ◽  
Jing Hao ◽  
Chun Yi ◽  
Dandan Zhang ◽  
Shengxiang Qiu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Liver Carcinoma ◽  
Scavenging Activity ◽  
Anticancer Activities

Antioxidant activities of wampee peel extracts using five different solvents (ethanol, hexane, ethyl acetate, butanol and water) were determined by using in-vitro antioxidant models including total antioxidant capability, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing power, and superoxide scavenging activity. Ethyl acetate fraction (EAF) exhibited the highest antioxidant activity compared to other fractions, even higher than synthetic antioxidant butylated hydroxyl toluene (BHT). In addition, the EAF exhibited strong anticancer activities against human gastric carcinoma (SGC-7901), human hepatocellular liver carcinoma (HepG-2) and human lung adenocarcinoma (A-549) cancer cell lines, higher than cisplatin, a conventional anticancer drug. The total phenolic content of wampee fraction was positively correlated with the antioxidant activity. This is the first report on the antioxidant and anticancer activities of the wampee peel extract. Thus, wampee peel can be used potentially as a readily accessible source of natural antioxidants and a possible pharmaceutical supplement.

scholarly journals Antioxidant activity of ethanolic extract of Penicillium chrysogenum and Penicillium fumiculosum

2014 ◽  
Vol 68 (1) ◽  
pp. 43-49 ◽  
Author(s):  
Violeta Jakovljevic ◽  
Jasmina Milicevic ◽  
Jelica Stojanovic ◽  
Slavica Solujic ◽  
Miroslav Vrvic
Keyword(s):  
Antioxidant Activity ◽  
Penicillium Chrysogenum ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Total Antioxidant ◽  
Chelating Ability

The aim of this study was to investigate the biological and chemical activity on two species of fungi of the genus Penicillium isolated from wastewater. On the selected species of fungi the different antioxidant activity assays were carried out: DPPH free-radical scavenging activity, total antioxidant activity, Fe2+- chelating ability and Fe3+- reducing power. Total phenol content was also determinate for ethanolic extract of mycelia. Penicillium chrysogenum ethanolic extract contained higher total phenolic content and better total antioxidant capacity as well as ferrous ion chelating ability. Penicillium fumiculosum ethanolic extract showed higher DPPH free-radical scavenging activity, as well as reducing power. Based on the obtained results it can be concluded that two types of fungi are potential new sources of natural antioxidants.

Investigation of the Anti-cataract and Antioxidant Activities of Cnidoscolus aconitifolius Leaves Extract In vitro

2021 ◽  
pp. 28-38
Author(s):  
Henry Bulama ◽  
Daniel Dahiru ◽  
Joshua Madu
Keyword(s):  
Total Protein ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Control Group ◽  
Scavenging Activity ◽  
Aqueous Fraction ◽  
Cnidoscolus Aconitifolius

Background: Cataract is a major cause of visual impairment and blindness around the world. This study evaluated the in vitro antioxidant and anti-cataract activities of Cnidoscolus aconitifolius leaves extract and fractions. Antioxidant activities were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzothiozoline-6-sulfonic acid) (ABTS), total reducing power, and hydrogen peroxide scavenging assays. Anti-cataract potential was evaluated in vitro using goat lenses divided into eight groups of different treatments and incubated in artificial aqueous humor at 37 °C for 72 hours. Glucose-induced opacity in the lenses was observed and biochemical indices quantified (catalase, Malondialdehyde (MDA) and total protein in the lens homogenate). Results: The crude extract and its fractions possess substantial antioxidant activities. The aqueous fraction exhibited the best DPPH radical scavenging activity (IC50 value 78.599 µg/ml); while the dichloromethane fraction exhibited the highest ABTS radical scavenging activity with IC50 66.68 µg/ml. The anti-cataract evaluation of crude and fractions at 250 μg/ml showed a significant increase (p<0.05) in the total protein and catalase activity compared to the cataract control group. The malonaldehyde level decreased significantly (p<0.05) in all the treated groups.

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