scholarly journals Effect of salicylic acid and yeast extract on caffeic acid derivatives production in Echinacea purpurea L.

2021 ◽  
Vol 20 (78) ◽  
pp. 36-47
Author(s):  
Mohammad Abdoli ◽  
Zhila Mehrpooya ◽  
Mohammad Reza Talebian ◽  
◽  
◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Caffeic Acid ◽  
Yeast Extract ◽  
Echinacea Purpurea ◽  
Caffeic Acid Derivatives

scholarly journals Increasing medicinal and phytochemical compounds of coneflower (Echinacea purpurea L.) as affected by NO3−/NH4+ ratio and perlite particle size in hydroponics

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Fatemeh Ahmadi ◽  
Abbas Samadi ◽  
Ebrahim Sepehr ◽  
Amir Rahimi ◽  
Sergey Shabala
Keyword(s):  
Particle Size ◽  
Caffeic Acid ◽  
Culture Media ◽  
Chemical Compounds ◽  
Echinacea Purpurea ◽  
Hydroponic Culture ◽  
Peat Moss ◽  
Root Extract ◽  
Caffeic Acid Derivatives ◽  
Phytochemical Compounds

AbstractMedicinal plants are considered as one of the most important sources of chemical compounds, so preparing a suitable culture media for medicinal plant growth is a critical factor. The present study is aimed to improve the caffeic acid derivatives and alkylamides percentages of Echinacea purpurea root extract in hydroponic culture media with different perlite particle size and NO3−/NH4+ ratios. Perlite particle size in the growing media was varied as very coarse perlite (more than 2 mm), coarse perlite (1.5–2 mm), medium perlite (1–1.5 mm), fine perlite (0.5–1 mm), and very fine perlite (less than 0.5 mm) in different ratios to peat moss (including pure perlite, 50:50 v/v, 30:70 v/v, and pure peat moss). Two NO3−/NH4+ ratios (90:10 and 70:30) were tested in each growing media. All phytochemical analyses were performed according to standard methods using high performance liquid chromatography (HPLC). It was found that the E. purpurea grown in the medium containing very fine-grade perlite with 50:50 v/v perlite to peat moss ratio had the maximum caffeic acid derivatives, including chicoric acid (17 mg g−1 DW), caftaric acid (6.3 mg g−1 DW), chlorogenic acid (0.93 mg g−1 DW), cynarin (0.84 mg g−1 DW), and echinacoside (0.73 mg g−1 DW), as well as, alkylamides (54.21%). The percentages of these phytochemical compounds increased by decreasing perlite particle size and increasing of NO3−/NH4+ ratio. The major alkylamide in the E. purpurea root extract was dodeca-2E, 4E, 8Z-10 (E/Z)-tetraenoic acid isobutylamide in all treatments, ranging from 31.12 to 54.21% of total dry weight. It can be concluded that optimizing hydroponic culture media and nutrient solution has significant effects on E. purpurea chemical compounds.

scholarly journals Patterns of variation in lipophilic and hydrophilic constituents in flower developmental stages of Echinacea purpurea (L.) Moench cultivated in Slovakia

2009 ◽  
Vol 55 (No. 2) ◽  
pp. 70-73 ◽  
Author(s):  
I. Mistríková ◽  
Š. Vaverková
Keyword(s):  
Developmental Stage ◽  
Caffeic Acid ◽  
Hplc Analysis ◽  
Developmental Stages ◽  
Echinacea Purpurea ◽  
Caffeic Acid Derivatives ◽  
Optimum Yield ◽  
The Third ◽  
Flower Age

The objective of this study was to examine and demonstrate how harvesting age (flower age) contribute to the variations in the quality of <i>Echinacea purpurea</i> (L.) Moench. The effects of different flower developmental stages on caffeic acid derivatives and isobutylamide content are described. These phytochemicals were extracted from fresh plants with 60% ethanol and quantified by the HPLC analysis. The results revealed that the quality of <i>Echinacea</i> is strongly influenced by the flower developmental stages. The highest content of both hydrophilic and lipophilic components in the anthodium of Echinacea plants were found in the third (mature) developmental stage, which is regarded as the optimum one for the harvest to obtain optimum yield levels.

scholarly journals Efficient extraction of caffeic acid derivatives from adventitious roots of Echinacea purpurea

2008 ◽  
Vol 26 (No. 4) ◽  
pp. 254-258 ◽  
Author(s):  
C.H. Wu ◽  
H.N. Murthy ◽  
E.J. Hahn ◽  
H.L Lee ◽  
K.Y. Paek
Keyword(s):  
Immune System ◽  
Herbal Medicine ◽  
Caffeic Acid ◽  
Adventitious Roots ◽  
Total Phenolics ◽  
Echinacea Purpurea ◽  
Food Supplements ◽  
Caffeic Acid Derivatives ◽  
Bioreactor Cultures ◽  
Efficient Extraction

<I>Echinacea</I> species are popular herbal medicine and food supplements for enhancing the immune system. This study was conducted with the aim of developing an efficient heat reflux extraction of caffeic acid derivatives from dried adventitious roots of <I>Echinacea purpurea</I> obtained in bioreactor cultures. Water, methanol (20, 40, 60, 80, and 100%), and ethanol (20, 40, 60, 80, and 100%) were used as solvents for the extraction of caffeic acid derivatives. Another parameter used for the optimisation was the solvent temperature during extraction. The treatment of samples with 60% ethanol at 60°C for 2 h proved to be the most suitable procedure. This treatment was also responsible for the higher yields of total phenolics, flavonoids, and polysaccharides.

Synergistic Effects of Thermal Pretreatment, Ultrasound and High-Pressure Processing on the Recovery of Bioactive Compounds from Echinacea Juice

2019 ◽  
Vol 18 (2) ◽  
pp. 144-151
Author(s):  
Fan Qingping ◽  
Hui Ailing ◽  
Zhang Wencheng ◽  
Fu Chuanxiang ◽  
Shen Lanlan ◽  
...  
Keyword(s):  
High Pressure ◽  
Caffeic Acid ◽  
Synergistic Effects ◽  
Echinacea Purpurea ◽  
Positive Control ◽  
High Pressure Processing ◽  
Control Group ◽  
Caffeic Acid Derivatives ◽  
Ultra High Pressure ◽  
Organ Index

Fresh Echinacea purpurea plant is a rich source of caffeic acid derivatives, which actively participate in the regulation of immune function. Herein, we describe a novel process for the production of fresh Echinacea purpurea juice rich in caffeic acid derivatives. This novel process includes steam heating (100°C, 15 min) before squeezing the juice to increase the recovery of caffeic acid derivatives from the flower or leaf by 1.5 to 3.7 fold. The recovery was further enhanced by 11–20% using ultrasonication at 500 W for 30 min. The flower, leaf, and root juice of Echinacea purpurea could be preserved for 4 weeks (4°C) or 6 months (−18°C) following ultra-high-pressure pasteurization (300 MPa, 15 min). Under these conditions, more than 90% of active cichoric acid was retained. Furthermore, the frozen-stored Echinacea purpurea juice exhibited similar or better immunoenhancement properties when measured by organ index, ear swelling, and carbon clearance test in mice compared with that of the positive control group (P < 0.05). Our results suggest that a combination of steam, ultrasound, and ultra-high-pressure treatment could serve as a superior process for the preparation of functional Echinacea purpurea juice.

scholarly journals Comparison of Maceration and Ultrasonication for Green Extraction of Phenolic Acids from Echinacea purpurea Aerial Parts

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5142
Author(s):  
Plamen Momchev ◽  
Petar Ciganović ◽  
Mario Jug ◽  
Eva Marguí ◽  
Jasna Jablan ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Phenolic Acids ◽  
Pharmaceutical Formulations ◽  
Echinacea Purpurea ◽  
Ultrasound Assisted Extraction ◽  
Glycerol Water ◽  
Caffeic Acid Derivatives ◽  
Beneficial Effects ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Echinacea purpurea is used in herbal medicinal products for the prevention and treatment of the common cold, as well as for skin disorders and minor wounds. In this study, the efficiency of traditional maceration using water and ethanol was compared with the maceration using mixtures of water and glycerol, a non-toxic, biodegradable solvent from renewable sources. It was found that the glycerol–water mixtures were as effective as ethanol/water mixtures for the extraction of caffeic acid derivatives. All the prepared extracts demonstrated notable antiradical properties. Furthermore, an efficient ultrasound-assisted extraction using glycerol–water mixtures was developed using six independent variables. Their levels needed for the maximum extraction of caffeic acid derivatives were as follows: glycerol 90% (m/m), temperature 70 °C, ultrasound power 72 W, time 40 min, and ascorbic acid 0 mg/mL. Under the optimized conditions, ultrasound-assisted extraction was superior to maceration. It achieved significantly higher yields of phenolic acids in shorter extraction time. The presence of zinc in plant material may contribute to the beneficial effects of E. purpurea preparations. Since glycerol is a non-toxic solvent with humectant properties, the prepared extracts can be directly used for the preparation of cosmetics or oral pharmaceutical formulations without the need for solvent removal.

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