scholarly journals Protective Effects of Olive Leaf Hydroalcoholic Extract on Sperm Parameters and Chromatin Quality in NMRI Mice Exposed to the Insecticide Mancozeb: An Experimental Study

2021 ◽  
Vol 20 (2) ◽  
pp. 147-162
Author(s):  
M. Ashkanani ◽  
H. Akbari ◽  
◽  
Keyword(s):  
Experimental Study ◽  
Sperm Parameters ◽  
Protective Effects ◽  
Olive Leaf ◽  
Hydroalcoholic Extract ◽  
Nmri Mice

scholarly journals L-Carnitine reduces the negative effects of formalin on sperm parameters, chromatin condensation and apoptosis in mice: An experimental study

2020 ◽  
Author(s):  
Daniyal Ezati ◽  
Reyhane Vardiyan ◽  
Ali Reza Talebi ◽  
Morteza Anvari ◽  
Majid Pourentezari
Keyword(s):  
Experimental Study ◽  
Chromatin Condensation ◽  
Sperm Parameters ◽  
Sperm Chromatin ◽  
Protective Effects ◽  
Negative Effects ◽  
Group Iii ◽  
Group I ◽  
And Control

Background: Formalin is commonly applied as an antiseptic and tissue fixative. It has reactive molecules that lead to its cytotoxic effects. According to recent studies, formalin causes a change in the testicular and sperm structure and L-carnitine (LC) acts as an antioxidant to counteract its effects. Objectives: This study aimed to investigate the protective effects of LC on the parameters, chromatin condensation and apoptosis of mice sperm exposed to formalin. Materials and Methods: In this experimental study, 24 balb/c mice (25-40 gr ,10-12 wk) were divided into three groups (n = 8/each): group I without any injections or gavage; group II, received 10 mg/ kg formalin intraperitoneally (I.P); and group III was exposed to formalin and LC, where a dose of 10 mg/kg formalin was injected I.P daily and LC the dose of 100 mg/kg was kept in a solvent solution. After 31 days, the sperm examination was performed as follows: to evaluate chromatin and DNA quality of the sperm, we applied aniline blue (AB), toluidine blue (TB), chromomycin A3 (CMA3), and terminal transferase-mediated deoxy uridine triphosphate biotin end labeling (TUNEL) tests. Results: Sperm parameters such as count, motility, morphology, and viability displayed a significant decrease in the formalin group. While the data exhibited a considerable augment in sperm parameters in the formalin + LC than the formalin and control groups (p < 0.001), significant differences were detected between groups with respect to TB staining, TUNEL test, CMA3 test and AB staining in the formalin and formalin + LC groups. Conclusion: LC can reduce the negative effects of formalin on sperm parameters, chromatin stability, and percentage of apoptosis in an animal model. Key words: Formalin, L-carnitine, Mice, Sperm chromatin, Apoptosis.

Protective Effects of Curcumin on Sperm and Stereological Parameters in Testes of Formaldehyde-Exposed NMRI Mice: An Experimental Study

2021 ◽  
Author(s):  
Elahe Fathi ◽  
Abbas Shahedi ◽  
Mohammad Hosseini Sharifabad ◽  
Mahmood Vakili
Keyword(s):  
Lipid Peroxidation ◽  
Group Iv ◽  
Sperm Parameters ◽  
Seminiferous Tubules ◽  
Cellular Injury ◽  
Protective Effects ◽  
Nmri Mice ◽  
Group Iii ◽  
Cell Numbers ◽  
Spermatogonia Cell

Background and Aims: Formaldehyde (FA) exposure is an important cause of cellular injury and oxidative damage in testis, leading to infertility. This study aimed to assess the protective effects of curcumin on sperm and stereological parameters in testes from formaldehyde-exposed NMRI mice. Materials and Methods: At 6-8 weeks of age, 24 adult male NMRI mice weighing 30-35 g were categorized into four groups (n=6) based on the treatment they received: Group І (control) received no treatment, group ΙΙ received FA (10 mg/kg), group ΙΙΙ received FA (10 mg/kg) and curcumin (100 mg/kg), and group IV (Solvent) received dimethyl sulfoxide (0.2 ml/day). Materials were administered intraperitoneally for 35 days. After excision, epididymis tissues were placed in 1 mL aliquots of Ham’s F10 medium at 37˚C for 20 min and were then used in analyses of sperm parameters. Testes were fixed and stained with Hematoxylin & Eosin to investigate stereological indices. We also determined lipid peroxidation levels using malondialdehyde assays. Results: Mean sperm parameters (count, motility, viability, and morphology) differed significantly between groups ΙΙ and ΙΙΙ (p≤0.001). Stereological indices, including Leydig and spermatogonia cell numbers and surface-to-volume ratios of seminiferous tubules were significantly higher in group ΙΙΙ than in group ΙΙ (p≤0.001) . Finally, malondialdehyde levels in group III were significantly lower than in group II (p=0.001). Conclusions: The data showed that the curcumin, as an antioxidant, reduced FA-induced damage in sperm parameters and stereological indices in mice testes.

Protective effects of icariin on dorsal random skin flap survival: An experimental study

2019 ◽  
Vol 861 ◽  
pp. 172600
Author(s):  
Guiqian Huang ◽  
Yuting Lin ◽  
Miaojie Fang ◽  
Dingsheng Lin
Keyword(s):  
Experimental Study ◽  
Skin Flap ◽  
Protective Effects ◽  
Flap Survival ◽  
Skin Flap Survival

Protective effects of the hydroalcoholic extract of Fumaria parviflora on testicular injury induced by torsion/detorsion in adult rats

Andrologia ◽  
2018 ◽  
Vol 50 (7) ◽  
pp. e13047 ◽  
Author(s):  
M. Shokoohi ◽  
H. Shoorei ◽  
M. Soltani ◽  
S.-H. Abtahi-Eivari ◽  
R. Salimnejad ◽  
...  
Keyword(s):  
Protective Effects ◽  
Adult Rats ◽  
Hydroalcoholic Extract ◽  
Testicular Injury

scholarly journals The protective effects of ketamine and propofol in obstructive jaundice: an experimental study

2012 ◽  
Vol 113 (03) ◽  
pp. 139-144
Author(s):  
H. Yildiz ◽  
I. Coskuner ◽  
E. Bulbuloglu ◽  
E. Silay ◽  
E. B. Kurutas ◽  
...  
Keyword(s):  
Experimental Study ◽  
Obstructive Jaundice ◽  
Protective Effects

scholarly journals The Protective Effect of Intratympanic Dexamethasone on Streptomycin Ototoxicity in Rats

2017 ◽  
Vol 96 (6) ◽  
pp. E12-E17 ◽  
Author(s):  
Aylin Göl ◽  
Engin Şengül ◽  
Beyhan Yılmaz ◽  
Fazıl Emre Özkurt ◽  
Mehmet Akdağ ◽  
...  
Keyword(s):  
Experimental Study ◽  
Hair Cells ◽  
Otoacoustic Emissions ◽  
Albino Rats ◽  
Protective Effects ◽  
Cochlear Hair Cells ◽  
Distortion Product ◽  
Dexamethasone Group ◽  
Significant Difference ◽  
Intratympanic Dexamethasone

The purpose of this experimental study was to investigate the protective role of intratympanically administered dexamethasone on the inner ears of rats that were exposed to streptomycin ototoxicity. Twenty-four adult Wistar albino rats were separated into 4 groups: Group 1 (only streptomycin), Group 2 (only intratympanic dexamethasone), Group 3 (streptomycin and intratympanic dexamethasone), and Group 4 (streptomycin and intratympanic saline). All rats were evaluated with distortion product otoacoustic emissions (DPOAE) tests before the start of treatment and on the day it ended. On the 45th day, after the final DPOAE tests, animals of all groups were sacrificed under general anesthesia. The differences between the amplitudes of DPOAE results were determined, and hearing results were statistically analyzed. Also, the cochleas of each rat were histopathologically evaluated under a light microscope with hematoxylin and eosin staining. In the intratympanic dexamethasone group it was observed that cochlear hair cells were mostly protected. No significant difference was seen between the DPOAE results before and after treatment (p > 0.05). On the other hand, loss was observed in the hearing functions and hair cells of the rats that received streptomycin and streptomycin plus intratympanic saline (p < 0.05). In the streptomycin plus intratympanic dexamethasone group, the cochlear hair cells were partially protected. A significant difference was observed when the DPOAE results (DP-grams) of the streptomycin plus intratypmanic dexamethasone group were compared to those of the streptomycin plus intratympanic saline group (p < 0.05). After the experimental study, ototoxic effects of the administration of streptomycin and intratympanic dexamethasone were observed on the rats’ cochlear hair cells. We conclude that intratympanic dexamethasone has protective effects against this cochlear damage in rats.

The protective effects of Ginkgo biloba extract (EGb-761) on radiation-induced dermatitis: an experimental study

2011 ◽  
Vol 37 (4) ◽  
pp. 387-394 ◽  
Author(s):  
E. Yirmibesoglu ◽  
E. Karahacioglu ◽  
D. Kilic ◽  
N. Lortlar ◽  
G. Akbulut ◽  
...  
Keyword(s):  
Experimental Study ◽  
Ginkgo Biloba ◽  
Ginkgo Biloba Extract ◽  
Protective Effects ◽  
Egb 761 ◽  
Radiation Induced
Sign in / Sign up

Export Citation Format

Share Document