Protective Effects of Curcumin on Sperm and Stereological Parameters in Testes of Formaldehyde-Exposed NMRI Mice: An Experimental Study

2021 ◽  
Author(s):  
Elahe Fathi ◽  
Abbas Shahedi ◽  
Mohammad Hosseini Sharifabad ◽  
Mahmood Vakili
Keyword(s):  
Lipid Peroxidation ◽  
Group Iv ◽  
Sperm Parameters ◽  
Seminiferous Tubules ◽  
Cellular Injury ◽  
Protective Effects ◽  
Nmri Mice ◽  
Group Iii ◽  
Cell Numbers ◽  
Spermatogonia Cell

Background and Aims: Formaldehyde (FA) exposure is an important cause of cellular injury and oxidative damage in testis, leading to infertility. This study aimed to assess the protective effects of curcumin on sperm and stereological parameters in testes from formaldehyde-exposed NMRI mice. Materials and Methods: At 6-8 weeks of age, 24 adult male NMRI mice weighing 30-35 g were categorized into four groups (n=6) based on the treatment they received: Group І (control) received no treatment, group ΙΙ received FA (10 mg/kg), group ΙΙΙ received FA (10 mg/kg) and curcumin (100 mg/kg), and group IV (Solvent) received dimethyl sulfoxide (0.2 ml/day). Materials were administered intraperitoneally for 35 days. After excision, epididymis tissues were placed in 1 mL aliquots of Ham’s F10 medium at 37˚C for 20 min and were then used in analyses of sperm parameters. Testes were fixed and stained with Hematoxylin & Eosin to investigate stereological indices. We also determined lipid peroxidation levels using malondialdehyde assays. Results: Mean sperm parameters (count, motility, viability, and morphology) differed significantly between groups ΙΙ and ΙΙΙ (p≤0.001). Stereological indices, including Leydig and spermatogonia cell numbers and surface-to-volume ratios of seminiferous tubules were significantly higher in group ΙΙΙ than in group ΙΙ (p≤0.001) . Finally, malondialdehyde levels in group III were significantly lower than in group II (p=0.001). Conclusions: The data showed that the curcumin, as an antioxidant, reduced FA-induced damage in sperm parameters and stereological indices in mice testes.

scholarly journals L-Carnitine reduces the negative effects of formalin on sperm parameters, chromatin condensation and apoptosis in mice: An experimental study

2020 ◽  
Author(s):  
Daniyal Ezati ◽  
Reyhane Vardiyan ◽  
Ali Reza Talebi ◽  
Morteza Anvari ◽  
Majid Pourentezari
Keyword(s):  
Experimental Study ◽  
Chromatin Condensation ◽  
Sperm Parameters ◽  
Sperm Chromatin ◽  
Protective Effects ◽  
Negative Effects ◽  
Group Iii ◽  
Group I ◽  
And Control

Background: Formalin is commonly applied as an antiseptic and tissue fixative. It has reactive molecules that lead to its cytotoxic effects. According to recent studies, formalin causes a change in the testicular and sperm structure and L-carnitine (LC) acts as an antioxidant to counteract its effects. Objectives: This study aimed to investigate the protective effects of LC on the parameters, chromatin condensation and apoptosis of mice sperm exposed to formalin. Materials and Methods: In this experimental study, 24 balb/c mice (25-40 gr ,10-12 wk) were divided into three groups (n = 8/each): group I without any injections or gavage; group II, received 10 mg/ kg formalin intraperitoneally (I.P); and group III was exposed to formalin and LC, where a dose of 10 mg/kg formalin was injected I.P daily and LC the dose of 100 mg/kg was kept in a solvent solution. After 31 days, the sperm examination was performed as follows: to evaluate chromatin and DNA quality of the sperm, we applied aniline blue (AB), toluidine blue (TB), chromomycin A3 (CMA3), and terminal transferase-mediated deoxy uridine triphosphate biotin end labeling (TUNEL) tests. Results: Sperm parameters such as count, motility, morphology, and viability displayed a significant decrease in the formalin group. While the data exhibited a considerable augment in sperm parameters in the formalin + LC than the formalin and control groups (p < 0.001), significant differences were detected between groups with respect to TB staining, TUNEL test, CMA3 test and AB staining in the formalin and formalin + LC groups. Conclusion: LC can reduce the negative effects of formalin on sperm parameters, chromatin stability, and percentage of apoptosis in an animal model. Key words: Formalin, L-carnitine, Mice, Sperm chromatin, Apoptosis.

scholarly journals Quality of Live Improvement Antituberculosis Consumer

2019 ◽  
Vol 2 (1) ◽  
pp. 22-25
Author(s):  
Jumasni Adnan
Keyword(s):  
Lipid Peroxidation ◽  
Liver Damage ◽  
Water Extract ◽  
Group Iv ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Male Wistar Rats ◽  
Group Ii

Antituberculosis is the most liver damage causes. Rifampicin and Isoniazide, in combination, are toxic compounds. Isoniazide and rifampicin metabolits causes lipid peroxidation. The hepatoprotective effect of rosella calyx water extract on liver damage induced with Isoniazide-rifampicin evaluated by examination of malondialdehid levels in the liver organ. 25 male wistar rats divided into 5 groups, ie group I (INH-rifampicin + rosella water extract 250 mg/kgBW), group II (INH-rifampicin + rosella water extract 125 mg/kgBW), group III (INH-rifampicin + rosella water extract 62.5 mg/kgBW),  group IV (healthy control) and group V (Isoniazide-rifampicin). MDA liver levels were analyzed after 35 days of treatments. The test results of each group are, group I has mean MDA levels 0.023912 + 0.011 mg/ml, group II 0.023526 + 0.009 mg/ml, group III 0.027168 + 0.007 mg/ml group IV 0.03437 + 0.009 mg/ml and group V 0.236846 + 0.118 mg/ml. The kruskal-wallis test showed significantly value 0.008 (p 0.05) and Post hoc Mann U whitney test showed that group V was significantly different to group I, II, III, and IV (p = 0.008) respectively, roselle extract can be used as a hepatoprotector antioxidant to improve the tuberculosis drug consumer quality of life through improved health by lowering lipid peroxidation that causes liver damage.

scholarly journals Effectiveness of the new polyfunctional infusion solution of blood substitutes on the activity of lipid peroxidation and antioxidant protection of heart in acute fatal blood loss

2016 ◽  
Vol 5 (1) ◽  
pp. 89
Author(s):  
Umid Ruziev ◽  
Khamid Karimov ◽  
Larisa Shevchenko ◽  
Timur Alimov
Keyword(s):  
Lipid Peroxidation ◽  
Blood Loss ◽  
Saline Solution ◽  
Blood Substitute ◽  
Group Iv ◽  
Blood Substitutes ◽  
Clinical Death ◽  
Group Iii ◽  
Group Ii ◽  
Lethal Blood

Background: One of the important directions of modern medicine is improving treatment of extreme conditions, the mortality rate of which is still very high, which is often associated with insufficient effectiveness of modern blood substitutes. Purpose of the study was to investigate the efficacy of a new multifunctional blood substitute in acute fatal blood loss on the activity of lipid peroxidation (LPO) and antioxidant system (AOS) of the heart, which showed its high antioxidant efficiency.Methods: Experiment was carried out on 60 rats weighing 180-200 g, clinical death was caused under etaminal anesthesia by acute blood loss from the carotid artery. After bleeding rats were given injection of infusions. Animals were divided into following groups: group I – before blood loss (intact), group II - clinical death, group III (control) – acute fatal blood loss after infusion of saline solution, group IV (comparison) - after acute lethal blood loss with infusion of reo-sorbilact, and group V (main, experimented) - after acute lethal blood loss with the new infusion of multifunctional blood substitute.Results: The level of MDA in clinical death (in group II) had increased by almost 2.0 times, as well as diene ketones, indicators of antioxidant activity decreased. The enzyme activity of GR in heart was 1.2 times lower, GPO - 1.5 times, SOD-2.0 times, catalase-4.4 times. In group III where animals after clinical death were revived with saline solution, AD increased to 45.6±0.4 mm Hg after 1 hour, and CBV increased to 44.8±0.4 ml/kg, the values of these parameters in the intact animals of the first group were 40.2% and 76.6%, respectively. After infusion of saline glomerular filtration rate was 0.61±0.02 ml/min, and diuresis of 0.21±0.02 ml/min compared with the values of these parameters in the intact animals was 46.9% and 65.6%, respectively. Life expectancy of animals after infusion of physiological saline was 12.3±1.2 hours, 30% of the animals survived. After infusion of reosorbilact in group IV, the survival rate was 40%, and after the infusion of a new multifunctional blood substitute in group IV -70%, which is 30% higherConclusions: The infusion of a new multifunctional blood substitute during acute fatal blood loss leads to a more effective delay of LPO processes and restoration of AOS in heart, in comparison with the use of reosorbilact. The use of a new multifunctional blood substitute during acute lethal hemorrhage in rats, compared with infusion of reosorbilact, leads to a more pronounced recovery of hemodynamic parameters, biochemical parameters of blood and ABS.

scholarly journals Pancreato-protective PANCREATOPROTECTIVE EFFECT OF SILYMARIN ON OXIDATIVE STRESS IN ALLOXAN-INDUCED HYPERGLYCEMIA IN MALE WISTAR RATS

2019 ◽  
pp. 121-125
Author(s):  
UMA NARAYANAMURTHY ◽  
SYLVIA SANTHAKUMARI A ◽  
NIRMALA P
Keyword(s):  
Lipid Peroxidation ◽  
Diabetic Complications ◽  
Wistar Rats ◽  
Serum Glucose ◽  
Pancreatic Tissue ◽  
Diabetic Rats ◽  
Group Iv ◽  
Group Iii ◽  
Group I ◽  
Male Wistar Rats

Objective: The objective of the study was to study the silymarin’s pancreatoprotective effect in alloxan-induced Type I diabetes mellitus. Numerous studies have evidence to prove the fact that antioxidant defense mechanism of flavonoids has overcome the progression of chronic diabetic complications. Methods: A total of 24 male Wistar rats were divided into four groups (n=6): Group I normal control, Group II, Group III, and Group IV were induced diabetes with alloxan. Group I and Group II diabetic rats received the vehicle (PO). Group III was treated with silymarin 400 mg/kg (PO). Group IV was treated with glibenclamide 0.5 mg/kg, per orally for 21 days. Fasting blood samples were collected from all four groups of animals at the end of 21 days to evaluate serum glucose and glycosylated hemoglobin (HbA1c). Pancreatic tissue extraction, to perform lipid peroxidation and histopathological study confirms the level of oxidative damage to tissues and recovery after treatment. Results: The serum glucose and HbA1c levels significantly increased in untreated diabetic rats, also a significant rise in lipid peroxidation and necrosis of beta cells in the pancreatic tissue. The rise in serum glucose levels was ameliorated in rats treated with silymarin, pancreatic tissue showed increased antioxidant levels, decreased lipid peroxides, and minimal changes and signs of regeneration of beta cells. Conclusion: This study adds experimental evidence to the fact that silymarin is an effective nutritional supplement to treasure pancreatic beta-cell reserve and to delay diabetic complications.

scholarly journals Possible Protective Effects of Omega 3, Diazepam and their Combination Against Yohimbine-Induced Clonic Seizure in Mice: Comparative Study

2021 ◽  
Vol 30 (2) ◽  
pp. 241-248
Author(s):  
Manal Abdulkhaliq Ibrahim ◽  
Alaa Radhi Khudhair ◽  
Nada Najia AL-shawi
Keyword(s):  
The United States ◽  
Clonic Seizure ◽  
Group Iv ◽  
Protective Effects ◽  
Omega 3 ◽  
Myoclonic Seizure ◽  
Standard Drug ◽  
Group Iii ◽  
Omega 3 Fatty Acid ◽  
Group I

     Yohimbine is actually confirmed in the United States to be utilized for erectile dysfunction; and recently such drug has become commonly used in body-building communities for its presumed lipolytic and sympathomimetic effects. But ingestion of such drug can bring about epileptic neurotoxic effects. Many antiepileptic drugs can be utilized to counteract myoclonic seizure; furthermore, diazepam can be used to oppose such type of seizure; in addition, surrogate therapeutic options such as omega 3 may also be utilized.   In this study, twenty-four (24) mice of both sexes weighing 20-25g were randomly-allocated into 4 groups (6 animals each group) as follows: Group I- Yohimbine-induced clonic seizure [mice orally-administered DMSO (10%), and after 30 min, animals Sc. injected with 45mg/kg yohimbine). Group II- Diazepam-treated as standard drug: It is Sc. injected at a dose of 2mg/kg, and after 30 minutes, yohimbine at a dose of 45mg/kg is Sc. injected. Group III- Omega 3 (40 mg/kg) is orally-administered, and after 30 min 45mg/kg yohimbine is Sc. injected. Group IV- Combination of omega 3 (40mg/kg) is orally-administered then and after ten minutes, diazepam was IP injected 2mg/kg then after 20 minute, 45mg/kg yohimbine is Sc. Injected. The result of this study showed that omega 3 has non-optimal antiepileptic effect; where, it is un-able to reduce the onset and frequency of epilepsy tone in mice during several time periods (30-120min); and data weren’t  significantly different when compared to diazepam. But omega 3 reduced onset of epilepsy in combination with diazepam when compared with diazepam alone.  As well as omega 3 caused small percent changes frequency of epilepsy tone through 120 min when compared with other groups. Conclusion: Omega 3 fatty acid had partial beneficial effect; where, alone it has a role for decreasing onset of epilepsy; but, its combination with diazepam had significant role for reducing onset of epilepsy against yohimbine-induced seizure model. Additionally, omega 3 alone and in combination with diazepam have negligible role for reduction frequency of epilepsy.

scholarly journals Magnesium Can Protect against Vanadium-Induced Lipid Peroxidation in the Hepatic Tissue

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Agnieszka Ścibior ◽  
Dorota Gołębiowska ◽  
Irmina Niedźwiecka
Keyword(s):  
Lipid Peroxidation ◽  
Group Iv ◽  
Hepatic Tissue ◽  
Control Group ◽  
Group Iii ◽  
Control Value ◽  
Group I ◽  
Value Range

The protective effect of magnesium as magnesium sulfate (MS) on sodium-metavanadate- (SMV-) induced lipid peroxidation (LPO) underin vivoandin vitroconditions was studied. The 18-week SMV intoxication (Group II, 0.125 /mL) enhanced spontaneous malondialdehyde (MDA) generation in rat liver, compared with the control (Group I) and MS-supplemented animals (Group III, 0.06 /mL). Coadministration of SMV with MS (Group IV, SMV-MS) caused a return of the MDA level to the control value range. The effect seems to result from the -independent action and its antagonistic interaction with . Thein vitrotreatment of liver supernatants (LS) obtained from all the tested animals groups with selected exogenous concentrations of or exhibited enhanced MDA production, compared with spontaneously formed MDA. It also showed -stimulating effect on LPO (LS I, Group I) and revealed that the changes in the MDA generation in LS IV (Group IV) might have resulted from the synergistic interactions of with and and from the antagonistic interactions of with and . The findings allow a suggestion that adequate Mg intake for a specific period in the conditions of SMV exposure may prevent V-induced LPO in the liver.

Pravastatin attenuates testicular damage induced by doxorubicin – a stereological and histopatological study

2018 ◽  
Vol 30 (1) ◽  
pp. 103-109 ◽  
Author(s):  
Bahram Eslami Farsani ◽  
Samaneh Karimi ◽  
Esrafil Mansouri
Keyword(s):  
Sperm Count ◽  
Saline Group ◽  
Group Iv ◽  
Seminiferous Tubules ◽  
Testicular Toxicity ◽  
Sprague Dawley ◽  
Group Iii ◽  
Group I ◽  
Sprague Dawley Rats ◽  
Tubule Diameter

Abstract Background The aim of this study is to investigate the effects of pravastatin (PS) against doxorubicin (DOX)-induced testicular toxicity. Methods A total of 24 healthy male Sprague-Dawley rats were equally divided into four groups. Group I received normal saline, Group II received PS (20 mg/kg b.w.) by gavage, Group III was treated with DOX alone (15 mg/kg b.w., i.p.) and Group IV received the combination of DOX and PS. Results After 8 weeks, the results displayed that DOX caused a decrease in testicular volume and index, epididymal sperm count, seminiferous tubule diameter and germinal epithelium. DOX also reduced the number of spermatogonia, spermatoctyes and Sertoli cells as well as increased the lumen diameter of seminiferous tubules (p<0.05) and the incidence of histopathological changes of the testis. Moreover, elevated malondialdehyde (MDA) levels and declined glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were observed (p<0.05). On the contrary, PS treatment significantly ameliorated nearly all of these abnormalities (p<0.05). Conclusions PS protects against DOX-induced testicular toxicity in rats, which is likely via the inhibition of oxidative stress and the increase of antioxidant enzyme activity.

scholarly journals Lipid Peroxidation, Antioxidative Defense and Level of 8-Hydroxy-2-Deoxyguanosine in Cervical Cancer Patients

2018 ◽  
Vol 37 (3) ◽  
pp. 336-345 ◽  
Author(s):  
Marija Jelić ◽  
Aljoša Mandić ◽  
Nebojša Kladar ◽  
Jan Sudji ◽  
Biljana Božin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cervical Cancer ◽  
Lipid Peroxidation ◽  
Thiobarbituric Acid ◽  
Antioxidant Defense System ◽  
Group Iv ◽  
Gas Chromatography Mass Spectrometry ◽  
Control Group ◽  
Group Iii ◽  
Group Ii

SummaryOxidative stress has been associated with cervical cancer. Our aim was to examine lipid peroxidation and the extent of oxidative stress in women diagnosed with different stages of cervical cancer in order to evaluate its potential role in the evolution of cancer. We measured the concentration of thiobarbituric acid reactive substances, activities of antioxidative enzymes and 8-hydroxy-2-deoxyguanosine in 153 subjects. Enzymatic activity as well as TBARS concentration were measured spectrophotometrically, while 8-OHdG was determined by gas chromatography-mass spectrometry. PPatients were categorized: group II H-SIL; group III FIGO Ia-Ib and group IV FIGO IIa-IV. Our results showed highly significant increase in the level of lipid peroxidation in group IV when com pared to the control group, group II and group III (p<0.001). Activity of superoxide dismutase was also significantly higher in group IV when compared to control group (p<0.01), group II (p<0.01) and group III (p<0.05). Activity of catalase was also significantly higher in group IV when compared to control group (p<0.005), group II (p<0.005) and group III (p<0.05). Activity of glutathione-S-transferase was also significantly higher in group IV when compared to control group (p<0.05), group II (p<0.05) and group III (p<0.05). Activities of glutathione peroxidase and glutathione reductase showed no significant differences among the groups. Level of 8-OHdG was significantly higher in group IV than in the other groups (p<0.01). It can be concluded that oxidative stress is possibly involved in the pathogenesis of cervical cancer, demonstrated by increased lipid peroxidation and an altered antioxidant defense system and higher levels of 8-OHdG.

Melatonin reduces high levels of lipid peroxidation induced by potassium iodate in porcine thyroid

2019 ◽  
pp. 1-7 ◽  
Author(s):  
Paulina Iwan ◽  
Jan Stepniak ◽  
Malgorzata Karbownik-Lewinska
Keyword(s):  
Lipid Peroxidation ◽  
Oxidative Damage ◽  
Membrane Lipids ◽  
Chemical Properties ◽  
Iodine Concentration ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Protective Effects ◽  
Potassium Iodate ◽  
Hormone Synthesis

Abstract. Iodine is essential for thyroid hormone synthesis. Under normal iodine supply, calculated physiological iodine concentration in the thyroid is approx. 9 mM. Either potassium iodide (KI) or potassium iodate (KIO3) are used in iodine prophylaxis. KI is confirmed as absolutely safe. KIO3 possesses chemical properties suggesting its potential toxicity. Melatonin (N-acetyl-5-methoxytryptamine) is an effective antioxidant and free radical scavenger. Study aims: to evaluate potential protective effects of melatonin against oxidative damage to membrane lipids (lipid peroxidation, LPO) induced by KI or KIO3 in porcine thyroid. Homogenates of twenty four (24) thyroids were incubated in presence of either KI or KIO3 without/with melatonin (5 mM). As melatonin was not effective against KI-induced LPO, in the next step only KIO3 was used. Homogenates were incubated in presence of KIO3 (200; 100; 50; 25; 20; 15; 10; 7.5; 5.0; 2.5; 1.25 mM) without/with melatonin or 17ß-estradiol. Five experiments were performed with different concentrations of melatonin (5.0; 2.5; 1.25; 1.0; 0.625 mM) and one with 17ß-estradiol (1.0 mM). Malondialdehyde + 4-hydroxyalkenals (MDA + 4-HDA) concentration (LPO index) was measured spectrophotometrically. KIO3 increased LPO with the strongest damaging effect (MDA + 4-HDA level: ≈1.28 nmol/mg protein, p < 0.05) revealed at concentrations of around 15 mM, thus corresponding to physiological iodine concentrations in the thyroid. Melatonin reduced LPO (MDA + 4-HDA levels: from ≈0.97 to ≈0,76 and from ≈0,64 to ≈0,49 nmol/mg protein, p < 0.05) induced by KIO3 at concentrations of 10 mM or 7.5 mM. Conclusion: Melatonin can reduce very strong oxidative damage to membrane lipids caused by KIO3 used in doses resulting in physiological iodine concentrations in the thyroid.

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