Isolation of Anemonin from Pulsatilla wallichiana and its Biological Activities

2019 ◽  
Vol 41 (2) ◽  
pp. 325-325
Author(s):  
Iftikhar Ali Iftikhar Ali ◽  
Sakeena Khatoon Sakeena Khatoon ◽  
Faiza Amber Faiza Amber ◽  
Qamar Abbas Qamar Abbas ◽  
Muhammad Ismail Muhammad Ismail ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Plant Sample ◽  
Spectroscopic Methods ◽  
Phytotoxic Activity ◽  
Whole Plant ◽  
Rh30 Cell ◽  
Reported Data ◽  
First Time

The phytochemical work on Pulsatilla wallichiana Ulbr. (Ranunculaceae Juss.) afforded a dilactone, anemonin (1) isolated, purified and identified by spectroscopic methods and comparison of their spectral data with the reported data. To the best of our knowledge, this compound was isolated for the first time from Pulsatilla wallichiana Ulbr. Moreover, the aqueous (aq.) methanolic extract of the whole plant sample of Pulsatilla wallichiana Ulbr. (PWW) and the methanolic extract of the whole plant sample of Physochlaina praealta (Decne.) Miers (Solanaceae Juss.; PPM) were also investigated for several biological activities including antifungal, antiinflammatory, anticancer (Rh30, Hela, 3T3), cytotoxic, phytotoxic, brine shrimp lethality and insecticidal activities. PWW showed insignificant antifungal while higher anti-inflammatory activity as compared to PPM. In anticancer activity, PWW showed a concentration dependent effect on Rh30 cell viability while both samples exhibited moderate phytotoxic activity.

Phytochemical Isolation and Biological Activities of Corydalis adiantifolia from Baltistan

2019 ◽  
Vol 41 (3) ◽  
pp. 535-535
Author(s):  
Iftikhar Ali Iftikhar Ali ◽  
Zulfiqar Ali Zulfiqar Ali ◽  
Alishba Alishba ◽  
Zamarrud Zamarrud ◽  
Hidayat Hussain and Viqar Uddin Ahmad Hidayat Hussain and Viqar Uddin Ahmad
Keyword(s):  
Plant Material ◽  
Reducing Sugars ◽  
Methanol Extract ◽  
Biological Activities ◽  
Spectroscopic Methods ◽  
Phytochemical Screening ◽  
Whole Plant ◽  
Hela Cell Lines ◽  
Water Fractions ◽  
First Time

The whole plant material of Corydalis adiantifolia Hook.f. andamp; Thomson was investigated for biological activities e.g. antifungal, antibacterial, insecticidal, cytotoxic, anticancer and phytotoxic activities and preliminary phytochemical screening. The methanol extract and dichloromethane fraction of the plant species exhibited 5% inhibition each against the fungus Aspergillus flavus. The methanol extract and water and dichloromethane fractions exhibited non-significant antibacterial activity and they showed inhibition against Hela cell lines and insignificant insecticidal activity. To understand the bioactive profile of C. adiantifolia, phytochemical screening approach is considered effective. The samples including methanol extract and n-hexane, dichloromethane, ethyl acetate and water fractions were subjected to qualitative phytochemical screening for the presence of various phytochemicals i.e. alkaloids, flavonoids, saponins, diterprenes, triterpenoids, anthraquinones, anthranol glycosides, reducing sugars and phenols. The results exhibited the efficacy of methanol extract showing the presence of more phytochemicals in comparison to the fractions of C. adiantifolia. Moreover, as a result of phytochemical isolation, 8-acetonyldihydrosanguinarine (1), β-sitosterol (2) and β-sitosterol-β-D-glucoside (3) were isolated, purified, and characterized by spectroscopic methods. To the best of our knowledge, all this study was carried out for the first time on C. adiantifolia.

Flavon- and Flavonolglycosides from Achillea pannonica Scheele

2001 ◽  
Vol 56 (7-8) ◽  
pp. 521-525 ◽  
Author(s):  
Denata Kasaj ◽  
Liselotte Krenn ◽  
Sonja Prinz ◽  
Antje Hüfner ◽  
Shi Shan Yuc ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
2D Nmr ◽  
Flavonoid Glycosides ◽  
Spectroscopic Methods ◽  
Esi Ms ◽  
Aerial Parts ◽  
Nmr Techniques ◽  
Chemotaxonomic Study ◽  
First Time ◽  
C Nmr

The detailed investigation of a methanolic extract of aerial parts of Achillea pannonica SCHEELE. within a chemotaxonomic study led to the isolation of 6 flavonoid glycosides. Besides rutin, apigenin-7-O-glucopyranoside, luteolin-7-O-glucopyranoside, apigenin-7-O-rutinoside and acacetin-7-O-rutinoside, an unusual flavondiglucoside was isolated. Its structure was established by UV, 1HNMR and 13C NMR spectroscopic methods including 2D-NMR techniques and ESI-MS as luteolin-7,4′-O-β-diglucoside. This substance is reported for the first time in the genus Achillea. Chemotaxonomic aspects are discussed briefly

Sesquiterpene Lactones from Elephantopus scaber

2005 ◽  
Vol 60 (2) ◽  
pp. 200-204 ◽  
Author(s):  
Ni Ni Than ◽  
Serge Fotso ◽  
Madhumati Sevvana ◽  
George M. Sheldrick ◽  
Heinz H. Fiebig ◽  
...  
Keyword(s):  
Single Crystal ◽  
Sesquiterpene Lactones ◽  
Spectroscopic Methods ◽  
X Ray ◽  
Whole Plant ◽  
Elephantopus Scaber ◽  
Ethyl Hexadecanoate ◽  
Reported Data ◽  
Acetone Extracts ◽  
Ethyl Octadecanoate

The ethanolic and acetone extracts of the whole plant of Elephantopus scaber were found to contain ethyl hexadecanoate, ethyl-9,12-octadecadienoate, ethyl-(Z)-9-octadecenoate, ethyl octadecanoate, lupeol, stigmasterol, stigmasterol glucoside, deoxyelephantopin (1) and two new germacranolide sesquiterpene lactones named 17,19-dihydrodeoxyelephantopin (2) and iso-17,19- dihydrodeoxyelephantopin (3) whose stereostructures were determined by spectroscopic methods, comparison with reported data and single-crystal X-ray analysis.

scholarly journals Chemical Constituents of the Root of Angelica tenuissima and their Anti-allergic Inflammatory Activity

2017 ◽  
Vol 12 (5) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Hyun Gyu Choi ◽  
In-Gyu Je ◽  
Geum Jin Kim ◽  
Joo-Won Nam ◽  
Sang Hee Shim ◽  
...  
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Methanolic Extract ◽  
Chemical Constituents ◽  
Inflammatory Activity ◽  
Plant Source ◽  
Chemical Structures ◽  
Human Mast Cells ◽  
Reported Data ◽  
First Time

A phthalide, levistolide A (1), and six coumarins, demethylsuberosin (2), fraxetin (3), (-)-marmesinin (4), 3'( S)- O-β-D-glucopyranosyl-3’,4'-dihydroxanthyletin (5), 3'( R)- O-β-D-glucopyranosyl-3’,4'-dihydroxanthyletin (6), and isopraeroside IV (7) were isolated from the methanolic extract of the roots of Angelica tenuissima Nakai. Their chemical structures were confirmed by comparing spectroscopic and reported data. All seven compounds were isolated for the first time from this plant source. The anti-allergic activities of compounds 1-7 were examined using human mast cells, and compounds 1-3 at 10 μM potently suppressed IL-6 expression and inhibited histamine release from human mast cells by more than 30%.

scholarly journals Analysis of the Essential Oils of Chamaemelum fuscatum (Brot.) Vasc. from Spain as a Contribution to Reinforce Its Ethnobotanical Use

Forests ◽  
2019 ◽  
Vol 10 (7) ◽  
pp. 539 ◽  
Author(s):  
Marcos Fernández-Cervantes ◽  
María José Pérez-Alonso ◽  
José Blanco-Salas ◽  
Ana Cristina Soria ◽  
Trinidad Ruiz-Téllez
Keyword(s):  
Essential Oils ◽  
Biological Activities ◽  
Folk Medicine ◽  
Gas Chromatography Mass Spectrometry ◽  
Distribution Area ◽  
Endemic Plant ◽  
Plant Materials ◽  
Whole Plant ◽  
First Time ◽  
Scientific Validation

Chamaemelum fuscatum (Brot.) Vasc. is a south west Iberian chamomile that has been traditionally used as folk medicine in its natural distribution area but currently it is underestimated regarding its biological activities. For this reason, it is proposed in this paper to get insight into the scientific validation of the traditional knowledge of this plant with the aim of taking advantage of its anti-inflammatory, gastroprotective and antinociceptive activities, among others. To this aim, the chemical composition of the essential oil from the whole plant, the flowers and the green parts of this plant has been evaluated by gas chromatography–mass spectrometry (GC–MS). Plant materials were collected in Badajoz (Spain). A total of 61 components including monoterpenoids, sesquiterpenoids and aliphatic esters were identified. (E)-2-Methyl-2-butenyl methacrylate (27.57%–18.53%) and 2-methylallyl isobutyrate (9.79%–7.51%) were the most abundant compounds in the essential oils of flowers and of the whole plant, whereas α-curcumene, trans-pinocarveol, α-bergamotene and pinocarvone were the major terpenoids irrespective of the plant part considered. Certain compounds showing a relative high abundance as isobutyl methacrylate, isoamyl butyrate, α-bergamotene and pinocarvone were identified for the first time in this species. Finally, we have reviewed the bioactivity of several compounds to relate the ethnobotanical use of this plant in Spain with its volatile profile. This work is a preliminary contribution to reinforce the use to this Mediterranean endemic plant as a natural source of bioactives.

scholarly journals Screening of antimicrobial potential and brine shrimp lethality bioassay of the whole plant extract of Spilanthes paniculata Wall. ex DC

2015 ◽  
Vol 3 (1) ◽  
pp. 1-5
Author(s):  
Rokeya Siddiqui ◽  
Md Masud Alam ◽  
Mohammad Ruhul Amin ◽  
AFM Shahid Ud Daula ◽  
MM Hossain
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Methanolic Extract ◽  
Brine Shrimp Lethality ◽  
Whole Plant ◽  
Vincristine Sulphate ◽  
Soluble Fractions

Bangladesh possesses a rich flora of medicinal plant. Out of the estimated 5000 species of phanerogams and pteridophytes growing in this country, more than a thousand are regarded as having medicinal properties. The aim of this study was to evaluate the in vitro antimicrobial activity and brine shrimp lethality of the whole plant extracts of Spilanthes paniculata, a plant belonging to the family Asteraceae. The crude extract, n-Hexane, chloroform and ethyl acetate soluble fraction of crude extract showed significant to moderate antimicrobial activity against three Gram positive and three Gram negative microorganisms. The zones of inhibition produced by the crude extract (methanolic extract), n-hexane, choloform and ethyl acetate soluble fractions were found to be 14.89 mm-19.40 mm, 14 mm-19.40 mm, 10.66 mm-13.50 mm and 9.50 mm-13.26 mm, consecutively at a concentration of 30 µg/disc. N-Hexane, chloroform and ethyl acetate soluble fractions of methanolic extract of Spilanthes paniculata were screened for antitumor properties using brine shrimp lethality bioassay. From the results of the brine shrimp lethality bioassay, it can be well predicted that n-hexane, chloroform and ethyl acetate soluble fractions of methanolic extract possess mild cytotoxicity on shrimp naupalii. The median lethal concentration (LC50, the concentration at which 50% mortality of brine shrimp nauplii occurred) of n-hexane, chloroform and ethyl acetate were 48.978 mg/ml, 92.61 mg/ml and 216.770 mg/ml, respectively, comparison with positive control vincristine sulphate with 0.839 mg/ml. DOI: http://dx.doi.org/10.3329/sjm.v3i1.22743 Stamford Journal of Microbiology, Vol.3(1) 2013: 1-5

scholarly journals Cytotoxic Sesquiterpene Lactones of Egyptian Tanacetum santolinoides

2007 ◽  
Vol 2 (8) ◽  
pp. 1934578X0700200 ◽  
Author(s):  
Diaa T. A. Youssef ◽  
Mahmoud A. Ramadan ◽  
Sabrin R. M. Ibrahim ◽  
Jihan M. Badr
Keyword(s):  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Nmr Spectra ◽  
Methanolic Extract ◽  
Soluble Fraction ◽  
Sesquiterpene Lactones ◽  
Natural Source ◽  
Mass Spectral ◽  
Aerial Parts ◽  
First Time

The chloroform soluble fraction of the methanolic extract of the aerial parts of Tanacetum santolinoides afforded six sesquiterpene lactones. Tanacetolide A (1) was isolated for the first time from a natural source, in addition to five known sesquiterpene lactones. The structures were established on the basis of extensive studies of their 1D and 2D (1H-1H COSY, HMQC, HMBC, HOHAHA and NOESY) NMR spectra and FAB mass spectral determinations. The compounds showed good cytotoxic activity when tested using the brine shrimp bioassay.

scholarly journals Flavonoids in Achillea collina

2001 ◽  
Vol 69 (1) ◽  
pp. 75-83 ◽  
Author(s):  
Denata Kasaj ◽  
Liselotte Krenn ◽  
Gottfried Reznicek ◽  
Sonja Prinz ◽  
Antje Hüfner ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
2D Nmr ◽  
Spectroscopic Methods ◽  
Esi Ms ◽  
Aerial Parts ◽  
First Time

In a detailed study on the flavonoid pattern of Achillea collina BECKER ten flavonoids were isolated from a methanolic extract of aerial parts of the plant. Their structures were determined by UV, ESI-MS, GC-MS and NMR spectroscopic methods including 2D-NMR. Apigenin-7-O-rutinoside was proven for the first time in the genus Achillea.

scholarly journals Phytotoxicity of Schiekia timida Seed Extracts, a Mixture of Phenylphenalenones

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4197
Author(s):  
Fernanda Maria Marins Ocampos ◽  
Ana Julia Borim de Souza ◽  
Guilherme Medeiros Antar ◽  
Felipe Christoff Wouters ◽  
Luiz Alberto Colnago
Keyword(s):  
Chemical Composition ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Germination Rate ◽  
Seed Extract ◽  
Hypocotyl Growth ◽  
Specialized Metabolites ◽  
Phytotoxic Effect ◽  
First Time ◽  
Seed Extracts

Phenylphenalenones, metabolites found in Schiekia timida (Haemodoraceae), are a class of specialized metabolites with many biological activities, being phytoalexins in banana plants. In the constant search to solve the problem of glyphosate and to avoid resistance to commercial herbicides, this work aimed to investigate the phytotoxic effect of the methanolic extract of S. timida seeds. The chemical composition of the seed extract was directly investigated by NMR and UPLC-QToF MS and the pre- and post-emergence phytotoxic effect on a eudicotyledonous model (Lactuca sativa) and a monocotyledonous model (Allium cepa) was evaluated through germination and seedling growth tests. Three concentrations of the extract (0.25, 0.50, and 1.00 mg/mL) were prepared, and four replicates for each of them were analyzed. Three major phenylphenalenones were identified by NMR spectroscopy: 4-hydroxy-anigorufone, methoxyanigorufone, and anigorufone, two of those reported for the first time in S. timida. The presence of seven other phenylphenalenones was suggested by the LC-MS analyses. The phenylphenalenone mixture did not affect the germination rate, but impaired radicle and hypocotyl growth on both models. The effect in the monocotyledonous model was statistically similar to glyphosate in the lowest concentration (0.25 mg/mL). Therefore, although more research on this topic is required to probe this first report, this investigation suggests for the first time that phenylphenalenone compounds may be post-emergence herbicides.

scholarly journals Chemical constituents isolated from the whole plant of Solanum procumbens

2019 ◽  
Vol 2 (6) ◽  
pp. 134-138
Author(s):  
Nguyen Xuan Hai ◽  
Nguyen Trung Nhan ◽  
Nguyen Thi Thanh Mai
Keyword(s):  
Salicylic Acid ◽  
Medicinal Plant ◽  
Benzoic Acid ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
Spectroscopic Methods ◽  
Chemical Structures ◽  
Whole Plant ◽  
Solanaceae Family ◽  
First Time

Solanum procumbens L. is a medicinal plant belonging to the Solanaceae family. In Vietnam, it is known as "Ca gai leo", and it cultivated in some tropical countries in China, Laos, Cambodia, and Vietnam. From the stem of the ethyl acetate extract of Solanum procumbens, we had isolated one anthraquinone (1), four polyphenols (2-5), and one indole (6). By spectroscopic methods as well as comparing with data in the literature, their chemical structures were elucidated as ziganein (1), benzoic acid (2), salicylic acid (3), 4-hydroxybenzaldehyde (4), vanillic acid (5), and indole-3-carbaldehyde (6). These compounds were isolated for the first time from the genus Solanum.

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