Development and Optimization of Green Method for Antihistamine Using Ecofriendly Reagent in Pure and Pharmaceutical Formulations by Microwave Assisted Spectrophotometry

2019 ◽  
Vol 41 (6) ◽  
pp. 1028-1028
Author(s):  
Amina Mumtaz Amina Mumtaz ◽  
Sajjad ul Hassan and Shafaq Mubarak Sajjad ul Hassan and Shafaq Mubarak
Keyword(s):  
Spectroscopic Method ◽  
Pharmaceutical Formulations ◽  
Ammonium Molybdate ◽  
Reaction Conditions ◽  
Green Method ◽  
Heating Systems ◽  
Microwave Assisted ◽  
Coloured Complex ◽  
Tablet Formulations

Synthetic substances and solvents utilized in investigation of drugs crumble the earth as well as human health. So attempts should be made to minimize or eliminate the utilization of obnoxious chemicals and solvents. The objective of study is to develop the green spectroscopic method using new ecofriendly chromogenic reagent for the evaluation of antihistamine i.e. desloratadine in pure and commercial dosage forms. Both heating systems (conventional and microwave assisted procedures) are used for the development of color. The method is based on formation of stable blue coloured complex with ammonium molybdate in the presence of acid having λmax at 732nm respectively. All the reaction conditions and different statistic parameters for the proposed methods have been studied. The method is found to be rapid, precise and accurate and can be successfully used for the determination of antihistamines in bulk and commercial tablet formulations.

scholarly journals Spectrophotometric Determination of Phosphate in Sugarcane Juice, Fertilizer, Detergent and Water Samples by Molybdenum Blue Method

2013 ◽  
Vol 11 (11) ◽  
pp. 58-62 ◽  
Author(s):  
Samjhana Pradhan ◽  
Megh Raj Pokhrel
Keyword(s):  
Hydrazine Hydrate ◽  
Sulphuric Acid ◽  
Ammonium Molybdate ◽  
Sugarcane Juice ◽  
Reaction Conditions ◽  
Molybdenum Blue ◽  
Scientific World ◽  
Coloured Complex ◽  
Phosphomolybdenum Blue

A simple and sensitive spectrophotometric method has been developed for the determination of phosphate in mg per liter (parts per million) concentration range in sugarcane juice, water, fertilizer and detergent samples. The amount of phosphate is determined by molybdenum blue phosphorus method in conjugation with UV-visible spectrophotometer. This method is based on the formation of phosphomolybdate complex with the added molybdate followed by the reduction of the complex with hydrazine hydrate in aqueous sulphuric acid medium. The system obeys Lambert-Beer’s law at 840 nm in the concentration range 0.1-11 ppm. The colour intensity of the reduced phosphomolybdate solution is found to be proportional to the amount of phosphate present in sugarcane juice, water, fertilizer and detergent samples. The reaction conditions as well as the various experimental parameters affecting the development and stability of the coloured complex were carefully investigated and optimized for the quantitative determination of phosphate present in various samples. The optimized concentrations of various reagents used are 0.20N sulphuric acid, 0.02M hydrazine hydrate and 0.20% ammonium molybdate. The effect of time on the formation of phosphomolybdenum blue complex and addition of the order of the reagents was also studied. Scientific World, Vol. 11, No. 11, July 2013, page 58-62DOI: http://dx.doi.org/10.3126/sw.v11i11.9139

scholarly journals Indirect spectrophotometric determination of gentamicin and vancomycin antibiotics based on their oxidation by potassium permanganate

2006 ◽  
Vol 4 (4) ◽  
pp. 708-722 ◽  
Author(s):  
Akram El-Didamony ◽  
Alaa Amin ◽  
Ahmed Ghoneim ◽  
Ayman Telebany
Keyword(s):  
Acid Medium ◽  
Potassium Permanganate ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Reaction Conditions ◽  
Subsequent Determination ◽  
Optimum Reaction ◽  
Acid Orange Ii ◽  
Sulphuric Acid Medium

AbstractFour simple, accurate, sensitive and economical procedures (A–D) for the estimation of gentamicin sulphate and vancomycin hydrochloride, both in pure form and in pharmaceutical formulations have been developed. The methods are based on the oxidation of the studied drugs by a known excess of potassium permanganate in sulphuric acid medium and subsequent determination of unreacted oxidant by reacting it with amaranth dye (method A), acid orange II (method B), indigocarmine (method C) and methylene blue (method D), in the same acid medium at a suitable λmax=521, 485, 610 and 664 nm, respectively. The reacted oxidant corresponds to the drug content. Regression analysis of Beer-Lambert plots showed good correlations in the concentration ranges 4–8, 3–8, 4–9 and 5–9 µg ml−1 with gentamicin and 4–8, 1.5–4, 1.5–4 and 3.5–5.5 µg ml−1 with vancomycin for methods A, B, C, and D, respectively. The molar absorptivity, sandell sensitivity, detection and quantification limits were calculated. The stoichiometric ratios for the cited drugs were studied. The optimum reaction conditions and other analytical parameters were evaluated. The influence of the substance commonly employed as excipients with these drugs were studied. The proposed methods were applied to the determination of these drugs in pharmaceutical formulations. The results have demonstrated that the methods are equally accurate and reproducible as the official methods.

Spectrophotometric Determination of Ephedrine Hydrochloride and Phenylephrine Hydrochloride

1982 ◽  
Vol 65 (4) ◽  
pp. 894-898
Author(s):  
Mohamed M Amer ◽  
Aly M Taha ◽  
Salwa R El-Shabouri ◽  
Pakinaz Y Khashaba
Keyword(s):  
Pharmaceutical Formulations ◽  
Eye Drops ◽  
Amine Group ◽  
Reaction Conditions ◽  
Phenylephrine Hydrochloride ◽  
Secondary Amine Group ◽  
Ephedrine Hydrochloride ◽  
Colored Product ◽  
The Relationship

Abstract A method is described for quantitative determination of the sympathomimetic amines ephedrine HCl and phenylephrine HCl. The method is based on the interaction of N-alkylvinylamine formed from the condensation of the free secondary amine group and acetaldehyde with chloranil to give a vinylaminosubstituted quinone. The colored product for ephedrine HCl and phenylephrine HCl exhibits 2 maximas at about 320 and 680 nm. All variables were studied to optimize reaction conditions. The relationship between absorbance and concentration was linear within 1-25 μg/mL under the conditions studied for both drugs at both wavelengths. The method has been applied to the analysis of some pharmaceutical formulations including tablets and eye drops with good recoveries (98.75-100.4%).

scholarly journals Pulse perturbation technique for determination of piroxicam in pharmaceuticals using an oscillatory reaction system

2013 ◽  
Vol 11 (2) ◽  
pp. 180-188 ◽  
Author(s):  
Nataša Pejić ◽  
Nataša Sarap ◽  
Jelena Maksimović ◽  
Slobodan Anić ◽  
Ljiljana Kolar-Anić
Keyword(s):  
Perturbation Technique ◽  
Kinetic Method ◽  
Reaction System ◽  
Direct Determination ◽  
Pharmaceutical Formulations ◽  
Oscillatory Reaction ◽  
Reaction Conditions ◽  
Good Sample ◽  
Pulse Perturbation

AbstractA simple and reliable novel kinetic method for the determination of piroxicam (PX) was proposed and validated. For quantitative determination of PX, the Bray-Liebhafsky (BL) oscillatory reaction was used in a stable non-equilibrium stationary state close to the bifurcation point. Under the optimized reaction conditions (T = 55.0°C, [H2SO4]0 = 7.60×10−2 mol L−1, [KIO3]0 = 5.90×10−2 mol L−1, [H2O2]0 = 1.50×10−1 mol L−1 and j 0 = 2.95×10−2 min−1), the linear relationship between maximal potential shift ΔE m , and PX concentration was obtained in the concentration range 11.2–480.5 µg mL−1 with a detection limit of 9.9 µg mL−1. The method had a rather good sample throughput of 25 samples h−1 with a precision RSD = 4.7% as well as recoveries RCV ≤ 104.4%. Applicability of the proposed method to the direct determination of piroxicam in different pharmaceutical formulations (tablets, ampoules and gel) was demonstrated.

scholarly journals Stability-indicating HPLC-PDA assay for simultaneous determination of paracetamol, thiamine and pyridoxal phosphate in tablet formulations

2019 ◽  
Vol 69 (2) ◽  
pp. 249-259 ◽  
Author(s):  
Amir Ali ◽  
Muhammad Makshoof Athar ◽  
Mahmood Ahmed ◽  
Kashif Nadeem ◽  
Ghulam Murtaza ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Pyridoxal Phosphate ◽  
Degradation Products ◽  
Ammonium Phosphate ◽  
Pharmaceutical Formulations ◽  
Hplc Method ◽  
Complete Separation ◽  
Coefficient Of Determination ◽  
Tablet Formulations

Abstract With the increased number of multi-drug formulations, there is a need to develop new methods for simultaneous determinations of drugs. A precise, accurate and reliable liquid chromatographic method was developed for simultaneous determination of paracetamol, thiamine, and pyridoxal phosphate in pharmaceutical formulations. Separation of analytes was carried out with an Agilent Poroshell C18 column. A mixture of ammonium phosphate buffer (pH = 3.0), acetonitrile and methanol in the ratio of 86:7:7 (V/V/V) was used as the mobile phase pumped at a flow rate of 1.8 mL min−1. Detection of all three components, impurities and degradation products was performed at the selected wavelength of 270 nm. The developed method was validated in terms of linearity, specificity, precision, accuracy, LOD and LOQ as per ICH guidelines. Linearity of the developed method was found in the range 17.5–30 µg mL−1 for thiamine, 35–60 µg mL−1 for pyridoxal phosphate and 87.5–150 µg mL−1 for paracetamol. The coefficient of determination was ≥ 0.9981 for all three analytes. The proposed HPLC method was found to be simple and reliable for the routine simultaneous analysis of paracetamol, thiamine and pyridoxal phosphate in tablet formulations. Complete separation of analytes in the presence of degradation products indicated selectivity of the method.

scholarly journals Indirect Spectrophotometric Methods for the Determination of Tadalafil

2015 ◽  
Vol 1 (2) ◽  
pp. 11 ◽  
Author(s):  
Safwan Mohammad Fraihat
Keyword(s):  
Oxidation Reaction ◽  
Indigo Carmine ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Blue Dye ◽  
Reaction Products ◽  
Reaction Conditions ◽  
Factors Affecting ◽  
Spectrophotometric Methods

Two spectrophotometric methods were developed for the determination Tadalafil in pharmaceutical preparations. The methods are based on the oxidation reaction with known excess amount of Ce(IV) and estimation of the unreacted amount using Indigo carmine dye (Method A) and in Methylene blue dye  (Method B). the factors affecting the reaction conditions were studied and the absorbance of absorbance of the oxidation reaction products were monitored at 610 and 600 nm for methods A and B respectively. Beer's law is obeyed in the concentration ranges 11 to 50 and 10 to 55 ppm, the limits of detection and quantification are reported. The proposed method was applied to the determination of the drug in pharmaceutical formulations and the results demonstrated that the method is equally accurate, precise and reproducible as the official methods. The validity of method was established by recovery studies with satisfactory results.

scholarly journals SPECTROFLUORIMETRIC DETERMINATION OF EOSIN CHEMILUMINESCENCE SYSTEM FOR THE ANALYSIS OF DABIGATRAN IN PHARMACEUTICAL FORMULATIONS

2019 ◽  
pp. 104-108
Author(s):  
DHANAPAL Y ◽  
SRUDHIVINOD V ◽  
MOHAMAD WASEEM A
Keyword(s):  
Method Development ◽  
Pharmaceutical Formulations ◽  
Calibration Graph ◽  
Eosin Y ◽  
Linear Calibration ◽  
Reaction Conditions ◽  
Quenching Effect ◽  
Spectrofluorimetric Determination ◽  
Optimum Reaction

Objectives: The objectives of this study were to develop a rapid, simple, and economical spectrofluorometric method for quantification of dabigatran from marketed formulation and its principle involved based on the protonated process of a binary mixture complex formation with eosin Y. Methods: A simple and precise spectrofluorometric technique was applied for the method development. It depends on measuring the quenching effect of the drug on the native fluorescence of eosin at excitation under the optimum reaction conditions. Results: The reaction linear calibration graph constructed between the fluorescence quenching valves flouresence intensity (ΔF) and the concentration ranges of 5–50 μg/ml. Spectrofluorometric analytical performance was validated by accuracy, precision, and specificity, and the results were satisfactory. Conclusion: This method was to develop a fast, simple, and economically applied successfully for the assay, and qualification of dabigatran tablet contains drug, also with different coformulated pharmaceutical formulations.

Validated Spectrophotometric Determination of Rizatriptan Benzoate in Pharmaceutical Formulations using Alizarin Derivatives

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
El Sheikh R ◽  
Hassan W. S. ◽  
Gouda A. A. ◽  
Al OwairdhiA. ◽  
Al Hassani K K H
Keyword(s):  
Standard Addition ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Optimum Conditions ◽  
Reaction Conditions ◽  
Alizarin Red ◽  
Rizatriptan Benzoate ◽  
Spectrophotometric Methods ◽  
Relative Standard

Two simple, sensitive, accurate, precise and economical spectrophotometric methods have been developed and validated for the determination of rizatriptan benzoate (RZT) in pure form and pharmaceutical formulations. These methods were based on the formation of charge transfer complex between RZT as n-electron donor and alizarin red S (ARS) or quinalizarin (Quinz) as π-acceptor in methanol to form highly colored chromogens which showed an absorption maximum at 532 and 574 nm using ARS and Quinz, respectively. The optimization of the reaction conditions such as the type of solvent, reagent concentration and reaction time were investigated. Under the optimum conditions, Beer’s law is obeyed in the concentration ranges 1.0-16 and 2.0-20 g mL-1 using ARS and Quinz, respectively with good correlation coefficient (r2 ≥ 0.9996) and with a relative standard deviation (RSD% ≤ 1.16). The molar absorptivity, Sandell sensitivity, detection and quantification limits were also calculated. The methods were successfully applied to the determination of RZT in its pharmaceutical formulations and the validity assesses by applying the standard addition technique. Results obtained by the proposed methods for the pure RZT and commercial tablets agreed well with those obtained by the reported method.

Flow‐Injection Spectrophotometric Determination of Dipyrone in Pharmaceutical Formulations Using Ammonium Molybdate as Chromogenic Reagent

2005 ◽  
Vol 38 (14) ◽  
pp. 2315-2326 ◽  
Author(s):  
Luiz H. Marcolino‐Júnior ◽  
Rogério A. Sousa ◽  
Orlando Fatibello‐Filho ◽  
Fernando C. Moraes ◽  
Marcos F.S. Teixeira
Keyword(s):  
Spectrophotometric Determination ◽  
Flow Injection ◽  
Pharmaceutical Formulations ◽  
Ammonium Molybdate ◽  
Chromogenic Reagent

scholarly journals Method Development and Validation for Determination of Voglibose in Tablet Formulation Using LC-MS/MS

2011 ◽  
Vol 8 (4) ◽  
pp. 1770-1783
Author(s):  
Mithlesh Rajput ◽  
Meenakshi Dahiya ◽  
Premlata Kumari ◽  
Kamini Kalra ◽  
Manjeet Aggarwal ◽  
...  
Keyword(s):  
Method Development ◽  
Limit Of Detection ◽  
Pharmaceutical Formulations ◽  
Limit Of Quantitation ◽  
Positive Mode ◽  
Method Development And Validation ◽  
Treatment Of Diabetes ◽  
Tablet Formulations ◽  
Development And Validation

Voglibose is a potent α glucosidase inhibitor, used for the treatment of diabetes mellitus. For quantitative determination of voglibose in pharmaceutical formulations of low doses, simple, sensitive, accurate and precise LC-MS/MS method using electrospray ionization in positive mode was developed and validated. The method was found linear in the concentration range of 25.0-1200 ηg/mL with a correlation coefficient of 0.9998. The limit of detection (LOD) of the method was found to be 1.5 ηg/mL and limit of quantitation (LOQ) was achieved at 3.0 ηg/mL. The recoveries of voglibose from spiked samples at different concentration levels were found in the range of 98-102%. The proposed method was found suitable for quantitation of voglibose and for the determination of uniformity of content of the dosage units of the tablet formulations.

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