Antibiotic susceptibility profile of extended spectrum β-lactamase producing Escherichia coli, Klebsiella pneumoniae and Klebsiella oxytoca from Urinary tract infections

2021 ◽  
pp. 4425-4428
Author(s):  
M. Sharmal Kumar ◽  
Arunagirinathan N. ◽  
Ravikumar M.
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antibiotic Susceptibility ◽  
Klebsiella Oxytoca ◽  
Bacterial Pathogen ◽  
Resistant Bacteria ◽  
Extended Spectrum ◽  
High Level ◽  
Tract Infections

The aim of this study was to analyze the extended spectrum β-lactamases (ESBLs) production and antibiotic susceptibility profile of urinary tract infected bacterial pathogens such as Escherichia coli and Klebsiella spp. A total of 143 Gram-negative bacteria were isolated from people suffering from urinary tract infections (UTIs) were included in this study. Among them, Escherichia coli (75%) were the predominantly isolated bacterial pathogen followed by Klebsiella oxytoca (14.6%) and K. pneumoniae (10.4%). Approximately 65% (n=93) of isolates were positive for ESBL production and E.coli was found to be the highest ESBL producer (67.6%) followed by K. oxytoca (57.1%) and K. pneumoniae (53.3%). E. coli showed high level of 86.1% resistance to cefotaxime and cefuroxime and 100% sensitive to imipenem and meropenem, whereas, K. oxytoca showed high level of 90.5% resistance to cefuroxime and 100% sensitive to amikacin, imipenem and meropenem. Similarly, K. pneumoniae showed high level of 73.3% resistance to nitrofurantoin and 93.3% sensitive to imipenem. This study reveals that majority of UTIs caused bacteria are ESBL producing multidrug-resistant bacteria and showing broad spectrum antibiotic resistance profile.

scholarly journals Prevalence of Extended Spectrum Beta-Lactamase Producing Bacteria (ESBL) in Patients presenting with Urinary Tract Infections (UTIs) at a peri-Urban hospital, Uganda

2020 ◽  
Author(s):  
Mugaga Abubakar ◽  
Nabuuma Josserine ◽  
Mubeezi Peruth ◽  
Onen Duncan ◽  
Aggrey Byaruhanga ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Health Center ◽  
Urinary Tract Infections ◽  
Resistant Bacteria ◽  
Beta Lactamase ◽  
Urban Hospital ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Tract Infections

Abstract Introduction Urinary tract infections remain one of the infections that are commonly diagnosed in Uganda with Enterobacteriaceae reported as the commonest uropathogens. Extended Spectrum Beta Lactamase producing Enterobacteriaceae have emerged as significant pathogens responsible for most of these urinary tract infections throughout the world. This resistance often leads to therapeutic failures from multidrug resistant bacteria. This study was carried out to determine the prevalence of Extended Spectrum Beta-Lactamase producing bacteria (ESBLs) in patients presenting with Urinary Tract Infections (UTIs) at Mbarara MunicipalCouncil Health Center IV, an urban hospital in Mbarara district, south western Uganda.Methods This was a Cross sectional study in which a total of 158 non repetitive midstream urine samples were obtained from 158 participants during months of June to August, 2018. Samples were cultured on conventional media (MacConkey agar and Cysteine Lysine Electrolyte Deficient agar) using a standard wire loop of capacity 0.05ml, incubated at 37 0 C for 24hrs. Only samples that showed significant growth (growth of pure colonies ≥105 cfu/ml) on primary culture were considered for further testing. The isolates were identified basing on the colony appearance, Gram stain and different conventional biochemical tests. Phenotypic expression of ESBLs production was detected using the double disc synergy test using Amoxicillin, Ceftazidime, Amoxicillin-Clavulanic acid and Ceftazidime-Clavulanic acid.Results The results show that 23% of the 158 participants had Urinary Tract infection. The most common uropathogens isolated were Staphylococcus aureus (64%) and Escherichia coli (17%). Phenotypic ESBLs was seen in 27.8% (10/36) of the uropathogens and was commonly seen among Escherichia coli , 60% (6/10) isolates. Others included; Pseudomonas species (8%), Enterobacteraerogenes (1.3%), Citrobacterfruendii (0.6%) and coagulase negative staphylococcus (0.6%).Conclusion This study revealed a fairly high prevalence of ESBL producing organisms in Mbarara Municipal Council Health Center IV and high levels of resistance to third generation cephalosporins.

scholarly journals Isolation of Extended-Spectrum β-lactamase- (ESBL-) Producing Escherichia coli and Klebsiella pneumoniae from Patients with Community-Onset Urinary Tract Infections in Jimma University Specialized Hospital, Southwest Ethiopia

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Mengistu Abayneh ◽  
Getnet Tesfaw ◽  
Alemseged Abdissa
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Klebsiella Pneumoniae ◽  
Clavulanic Acid ◽  
Urinary Tract Infections ◽  
Southwest Ethiopia ◽  
Extended Spectrum ◽  
Specialized Hospital ◽  
Amoxicillin Clavulanic Acid ◽  
Tract Infections

Background. Klebsiella pneumoniae and Escherichia coli are the major extended-spectrum β-lactamase- (ESBL-) producing organisms increasingly isolated as causes of complicated urinary tract infections and remain an important cause of failure of therapy with cephalosporins and have serious infection control consequence. Objective. To assess the prevalence and antibiotics resistance patterns of ESBL-producing Escherichia coli and Klebsiella pneumoniae from community-onset urinary tract infections in Jimma University Specialized hospital, Southwest Ethiopia, 2016. Methodology. A hospital-based cross-sectional study was conducted, and a total of 342 urine samples were cultured on MacConkey agar for the detection of etiologic agents. Double-disk synergy (DDS) methods were used for detection of ESBL-producing strains. A disc of amoxicillin + clavulanic acid (20/10 µg) was placed in the center of the Mueller–Hinton agar plate, and cefotaxime (30 µg) and ceftazidime (30 µg) were placed at a distance of 20 mm (center to center) from the amoxicillin + clavulanic acid disc. Enhanced inhibition zone of any of the cephalosporin discs on the side facing amoxicillin + clavulanic acid was considered as ESBL producer. Results. In the current study, ESBL-producing phenotypes were detected in 23% (n = 17) of urinary isolates, of which Escherichia coli accounts for 76.5% (n = 13) and K. pneumoniae for 23.5% (n = 4). ESBL-producing phenotypes showed high resistance to cefotaxime (100%), ceftriaxone (100%), and ceftazidime (70.6%), while both ESBL-producing and non-ESBL-producing isolates showed low resistance to amikacin (9.5%), and no resistance was seen with imipenem. In the risk factors analysis, previous antibiotic use more than two cycles in the previous year (odds ratio (OR), 6.238; 95% confidence interval (CI), 1.257–30.957; p = 0.025) and recurrent UTI more than two cycles in the last 6 months or more than three cycles in the last year (OR, 7.356; 95% CI, 1.429–37.867; p = 0.017) were found to be significantly associated with the ESBL-producing groups. Conclusion. Extended-spectrum β-lactamases- (ESBL-)producing strain was detected in urinary tract isolates. The occurrence of multidrug resistance to the third-generation cephalosporins, aminoglycosides, fluoroquinolones, trimethoprim-sulfamethoxazole, and tetracyclines is more common among ESBL producers. Thus, detecting and reporting of ESBL-producing organisms have paramount importance in the clinical decision-making.

scholarly journals Relative Fecal Abundance of Extended-Spectrum-β-Lactamase-Producing Escherichia coli Strains and Their Occurrence in Urinary Tract Infections in Women

2013 ◽  
Vol 57 (9) ◽  
pp. 4512-4517 ◽  
Author(s):  
Etienne Ruppé ◽  
Brandusa Lixandru ◽  
Radu Cojocaru ◽  
Çağrı Büke ◽  
Elisabeth Paramythiotou ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Virulence Factors ◽  
Urinary Tract Infections ◽  
Predictive Values ◽  
Content Type ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Tract Infections

ABSTRACTExtended-spectrum-beta-lactamase (ESBL)-producingEscherichia coli(ESBLE. coli) strains are of major concern because few antibiotics remain active against these bacteria. We investigated the association between the fecal relative abundance (RA) of ESBL-producingE. coli(ESBL-RA) and the occurrence of ESBLE. coliurinary tract infections (UTIs). The first stool samples passed after suspicion of UTI from 310 women with subsequently confirmedE. coliUTIs were sampled and tested for ESBL-RA by culture on selective agar. Predictive values of ESBL-RA for ESBLE. coliUTI were analyzed for women who were not exposed to antibiotics when the stool was passed. ESBLE. coliisolates were characterized for ESBL type, phylogroup, relatedness, and virulence factors. The prevalence of ESBLE. colifecal carriage was 20.3%, with ESBLE. coliUTIs being present in 12.3% of the women. The mean ESBL-RA (95% confidence interval [CI]) was 13-fold higher in women exposed to antibiotics at the time of sampling than in those not exposed (14.3% [range, 5.6% to 36.9%] versus 1.1% [range, 0.32% to 3.6%], respectively;P< 0.001) and 18-fold higher in women with ESBLE. coliUTI than in those with anotherE. coliUTI (10.0% [range, 0.54% to 100%] versus 0.56% [range, 0.15% to 2.1%[, respectively;P< 0.05). An ESBL-RA of <0.1% was 100% predictive of a non-ESBLE. coliUTI. ESBL type, phylogroup, relatedness, and virulence factors were not found to be associated with ESBL-RA. In conclusion, ESBL-RA was linked to the occurrence of ESBLE. coliUTI in women who were not exposed to antibiotics and who had the same clone ofE. coliin urine samples and fecal samples. Especially, a low ESBL-RA appeared to be associated with a low risk of ESBLE. coliinfection.

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