Serum Copper and Zinc Levels of Oral Submucous Fibrosis Patients Using Areca Nut Products Reported at Isra University Hospital Hyderabad

Objective: To determine the copper and zinc levels in serum among oral submucous fibrosis patients using various areca nut products by taking healthy individuals as control at Isra University Hospital Hyderabad. Methodology: This descriptive case-control study was conducted on patients of oral submucosal fibrosis (OSMF) attending the Outpatient Department, Oral Surgery Isra Dental College, Isra university during a period of one year from November 2016 to October 2017. A total of 30 patients above 16 years of age and who were diagnosed cases of OSMF and gave history of areca nut and its commercial products consumption for more than one year were included as group (B)and in equal number healthy individuals were enrolled as control as group(A). The 6ml blood sample was collected in gel tube and then we centrifuged at 3000 rpm gravity for 10min to separate the serum from blood. The 4 to 5ml serum collected in this way was analyzed by spectrophotometric method on Hitachi 902(Roche Germany). The levels of copper and zinc were estimated from serum of both groups. All the data was collected via study proforma and analysis of the data was done by using SPSS version 20. Results: Mean age of the patients was 23.33±4.97 years and mean age of healthy controls was 22.80±4.61 years. The males were most common in both groups. Manipuri was commonest consumed areca nut (50.0%) followed by sweet scented areca nut and Gutkha 33.3% and 36.6% respectively. Mean of serum Cu in patients was lower 86±4.20 µg/dl in contrast to controls 114.0±13.8 µg/dl (p-0.0001). Average of serum Zinc was higher among patients 94.20±15.11 µg/dl as compared to controls as 68.06±17.10 µg/dl (p-0.0001). Conclusion: The average of serum copper level was observed to be significantly less and average of serum zinc significantly higher among OSMF patients using areca nut as compared to healthy individuals. These serum trace element levels could be used as potential prognostic and diagnostic markers in the OSMF patients. Key words: OSMF, Zinc, Copper, Areca nut

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ESTIMATION OF SERUM COPPER AND ZINC IN ANAEMIC PATIENTS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2016.v9i5.12892 ◽

2016 ◽

Vol 9 (5) ◽

pp. 162

Author(s):

Aishwarya Swaminathan ◽

Sohara Parveen ◽

Vishnupriya V ◽

Gayathri R

Keyword(s):

Study Design ◽

Clinical Laboratory ◽

Serum Zinc ◽

Serum Copper ◽

Healthy Individuals ◽

Adequate Amount ◽

Rich Food ◽

Copper And Zinc ◽

Group 2 ◽

Zinc Levels

ABSTRACTObjective: Estimation of serum zinc and copper levels in anemic patients.Methods: The study design included 30 subjects presenting to clinical laboratory, global hospitals. The subjects were divided into 2 groups. TheGroup 1 consists of 15 healthy individuals and Group 2 consists of 15 anemic patients.Results: The serum copper and zinc levels were evaluated in anemic patients. The serum copper and zinc were found to be significantly lower inanemic patients when compared with normal individuals.Conclusion: Thus, the study concludes that the serum zinc and copper in anemic patients is significantly less when compared to the control individuals.Hence, it is important to add adequate amount of zinc and copper rich food in their diet to control the prevalence of anemia.Keywords: Anemia, Zinc, Copper, Hemoglobin.

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Estimation of Serum Copper and Zinc Levels in Oral Submucous Fibrosis: An Atomic Absorption Spectroscopic Study

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1406 ◽

2013 ◽

Vol 14 (5) ◽

pp. 801-805 ◽

Cited By ~ 2

Author(s):

H Neethi

Keyword(s):

Spectroscopic Study ◽

Atomic Absorption ◽

Oral Submucous Fibrosis ◽

Serum Copper ◽

Stage I ◽

Healthy Controls ◽

Copper And Zinc ◽

Submucous Fibrosis ◽

The Mean ◽

Zinc Levels

ABSTRACT Background and objectives Biochemical derangements of microelements although reported in oral cancer is poorly understood in oral submucous fibrosis (OSMF). Hence, the present study was carried out to estimate and compare the serum copper and zinc levels among different histopathological stages of OSMF with that of healthy controls. Materials and methods Thirty histopathologically diagnosed cases of OSMF and 30 healthy controls reporting to MS Ramaiah Dental College and Hospital (MSRDCH), Bengaluru, were included in the study. The histopathological staging of OSMF was done as per criteria given by Pindborg and Sirsat (1966). Blood samples were collected and the serum copper and zinc levels were estimated using atomic absorption spectroscopy. The results were statistically analyzed using independent t-test and Kruskal-Wallis test. Results The mean serum copper levels were increased (p < 0.0005) and zinc levels were decreased (p < 0.01) in OSMF patients when compared to that of controls. The mean serum copper levels showed an increasing trend from stage I to IV (p = 0.004) while zinc levels showed a decreasing trend from stage I to IV (p = 0.04) in OSMF patients. Conclusion The serum copper and zinc levels in OSMF showed a significant difference in comparison to that of controls and between the histopathological stages. These findings indicate that serum copper and zinc may have a contributory role in the etiopathogenesis of OSMF. Clinical relevance Although the role of the trace elements as etiological factors is minor, derangements noted in the serum copper and zinc levels could be correlated with diseased progression and possibly explain the transformation of OSMF into malignancy. Thus, they can be used as prognostic markers and can be of value for proactive intervention. How to cite this article Neethi H, Patil S, Rao RS. Estimation of Serum Copper and Zinc Levels in Oral Submucous Fibrosis: An Atomic Absorption Spectroscopic Study. J Contemp Dent Pract 2013;14(5):801-805.

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Estimation of Serum Copper and Zinc in Patients of Oral Submucous Fibrosis in Rural Population

World Journal of Dentistry ◽

10.5005/jp-journals-10015-1773 ◽

2020 ◽

Vol 11 (6) ◽

pp. 478-481

Author(s):

Amol Gadbail ◽

Alka Hande ◽

Anand V Bansod ◽

Akhilesh G Agrawal ◽

Amit M Reche

Keyword(s):

Rural Population ◽

Oral Submucous Fibrosis ◽

Serum Copper ◽

Copper And Zinc ◽

Submucous Fibrosis

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Analysis of Serum and Urinal Copper and Zinc in Chinese Northeast Population with the Prediabetes or Diabetes with and without Complications

Oxidative Medicine and Cellular Longevity ◽

10.1155/2013/635214 ◽

2013 ◽

Vol 2013 ◽

pp. 1-11 ◽

Cited By ~ 30

Author(s):

Jiancheng Xu ◽

Qi Zhou ◽

Gilbert Liu ◽

Yi Tan ◽

Lu Cai

Keyword(s):

Serum Zinc ◽

Zinc Level ◽

Serum Copper ◽

Copper Level ◽

Simvastatin Treatment ◽

Copper And Zinc ◽

Urinary Copper ◽

Zinc Levels

This study investigated the association of copper and zinc levels in the serum or urine of patients living in northeast China, with either prediabetes or diabetes. From January 2010 to October 2011, patients with type 1 diabetes (T1D,n=25), type 2 diabetes (T2D,n=137), impaired fasting glucose (IFG,n=12) or impaired glucose tolerance (IGT,n=15), and age/gender matched controls (n=50) were enrolled. In the T2D group, there were 24 patients with nephropathy, 34 with retinopathy, and 50 with peripheral neuropathy. Serum copper levels were significantly higher in IFG, IGT, and T2D groups. Serum zinc level was dramatically lower, and urinary zinc level was significantly higher in both T1D and T2D subjects compared with controls. The serum zinc/copper ratio was significantly lower in all the patients with IFG, ITG, T1D, and T2D. The serum copper level was positively associated with HbA1c in T2D subjects. Simvastatin treatment in T2D patients had no significant effect on serum and urinary copper and zinc. These results suggest the need for further studies of the potential impact of the imbalanced serum copper and zinc levels on metabolic syndrome, diabetes, and diabetic complications.

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Serum Copper and Zinc Levels and Risk of Hepatocellular Carcinoma: A 1:1 Matched Case-Control Study

Current Developments in Nutrition ◽

10.1093/cdn/nzaa061_025 ◽

2020 ◽

Vol 4 (Supplement_2) ◽

pp. 1397-1397

Author(s):

Aiping Fang ◽

Huilian Zhu

Keyword(s):

Serum Zinc ◽

Serum Levels ◽

Case Control ◽

Serum Copper ◽

Copper And Zinc ◽

Copper Zinc ◽

Matched Case ◽

Zinc Levels ◽

Control Study ◽

Serum Zinc Levels

Abstract Objectives Copper and zinc have been involved in the development of liver cancer in laboratory models, yet data from epidemiological studies are limited. We investigated the association of serum levels of copper and zinc and their ratio with the risk of hepatocellular carcinoma (HCC) in a Chinese population. Methods This is a 1:1 matched case-control study conducted between September 2013 and February 2017. Each case with newly diagnosed HCC from the Guangdong Liver Cancer Cohort (GLCC) was matched to one healthy control from the Guangzhou Nutrition and Health Study by age (±1 years) and sex. Serum copper and zinc levels were quantified using inductively coupled plasma mass spectrometry. Conditional logistic regression models were used to estimate odds ratios (ORs) and 95% confidence intervals (CIs) adjusted for age, sex, body mass index, hepatitis infection, smoking status, alcohol drinking status, diabetes, and chronic inflammation. Serum levels of copper and zinc and their ratio were included in models in tertiles with cutpoints based on the distribution of the control participants. Results A total of 556 case-control pairs were included in this study. Mean (SD) age was 60.3 (7.1) years and 83.8% of the participants were men. HCC cases had significantly higher copper and lower zinc levels, and thus higher copper/zinc, than controls. Higher serum zinc levels were associated with a lower risk of HCC (ORT3 vs T1 = 0.21; 95% CI: 0.07–0.64, P for trend = 0.005). However, there was no association between serum copper levels and HCC risk (ORT3 vs T1 = 1.53; 95% CI: 0.62–3.76, P for trend = 0.375). Higher copper/zinc ratio was associated with increased risk of HCC (ORT3 vs T1 = 3.06; 95% CI: 1.08–8.70, P for trend = 0.049). Conclusions Serum zinc levels were inversely associated with the risk of HCC, suggesting a protective role of zinc in preventing HCC development. Funding Sources This work was supported by the National Natural Science Foundation of China.

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Diurnal Variation of Serum Copper and Zinc in Epileptics Receiving Anti-Convulsants

Human Toxicology ◽

10.1177/096032718600500308 ◽

1986 ◽

Vol 5 (3) ◽

pp. 195-200 ◽

Cited By ~ 9

Author(s):

A. Taylor ◽

K. Ghose

Keyword(s):

Diurnal Variation ◽

Reference Range ◽

Serum Zinc ◽

Serum Copper ◽

Copper Level ◽

Copper And Zinc ◽

Urinary Copper ◽

Zinc Concentrations ◽

Zinc Levels ◽

Patients With Epilepsy

1 Serial measurements of copper and zinc concentrations in serum were made at 06.00, 14.00, 22.00 and again at 06.00 hours in 37 male patients with epilepsy, aged between 9 and 19 years. Anti-convulsant drugs were administered at 08.00 and 20.00 hours, and standard hospital meals were allowed at 07.30, 12.00, 16.00 and 19.00 hours. 2 Similar to our previous results, eight patients (21.6%) had serum copper levels greater than the reference range (11.0–20.5 μmol/l) and this hypercupraemia was associated with carbamazapine and/or phenytoin medication. 3 No diurnal variation in serum copper level was observed. Serum copper concentration had no correlation with either 24 h urinary copper excretion or serum anti-convulsant drug levels. 4 Serum zinc concentrations were within the reference range (10–16.5 μmol/l), confirming our previous report. No relation with anti-convulsant medication or serum copper levels was found. 5 Diurnal variations in serum zinc levels with peak and trough concentrations at 06.00 and 14.00 hours, respectively, were observed. 6 It is proposed that these variations in serum zinc concentrations are a normal physiological process and is unlikely to be related to anti-convulsant drugs or epilepsy.

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Analysis of Copper and Zinc Levels in the Mucosal Tissue and Serum of Oral Submucous Fibrosis Patients

World Journal of Dentistry ◽

10.5005/jp-journals-10015-1015 ◽

2010 ◽

Vol 1 (2) ◽

pp. 75-78 ◽

Cited By ~ 2

Author(s):

Ajay G Nayak ◽

Laxmikanth Chatra ◽

K Prashanth Shenai

Keyword(s):

Trace Elements ◽

Oral Mucosa ◽

Oral Submucous Fibrosis ◽

Zinc Level ◽

Copper Level ◽

Pathogenetic Mechanisms ◽

Copper And Zinc ◽

Submucous Fibrosis ◽

The Mean ◽

Zinc Levels

ABSTRACT Background and Objectives Oral submucous fibrosis (OSMF) is a well-recognized potentially malignant disease of oral mucosa whose exact pathogenetic mechanisms have not been understood. Measurement of copper and zinc levels in the tissue and serum of these patients may be helpful in understanding the pathogenesis. Therefore, a study was undertaken to analyze the levels of these trace elements in the populations of the coastal region of south Karnataka and north Kerala, India, who show predominant use of areca nut due to their cultural and social backgrounds. Methods A case-control study was conducted on 20 cases of OSMF and 20 controls. The tissue and serum concentrations of copper and zinc in these 40 subjects were measured by colorimetry. Results The mean tissue copper level in OSMF group was 4.31 ± 1.13 μg/gm, while the tissue zinc level was 25.18 ± 4.92 μg/gm. The mean serum copper level in the OSMF group was 1.00 ± 0.20 μg/ml while the mean serum zinc level was 0.92 ± 0.18 μg/ml. Interpretation and conclusion The tissue copper levels in OSMF group showed very highly significant difference (p = 0.001) from the controls while the difference in tissue zinc levels was highly significant (p = 0.009). The serum levels of copper (p = 0.35) and zinc (p = 0.08) did not exhibit any statistical difference from those of the control group. These findings indicate that the trace elements, copper and zinc, within the oral mucosa definitely, have a role to play in the pathogenetic mechanisms of OSMF.

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Role of Hemoglobin and Serum Iron in Oral Submucous Fibrosis: A Clinical Study

The Scientific World JOURNAL ◽

10.1100/2012/254013 ◽

2012 ◽

Vol 2012 ◽

pp. 1-5 ◽

Cited By ~ 5

Author(s):

Hegde Karthik ◽

Preeti Nair ◽

Harshkant P. Gharote ◽

Kavita Agarwal ◽

Guruprasad Ramamurthy Bhat ◽

...

Keyword(s):

Healthy Subjects ◽

Serum Iron ◽

Oral Submucous Fibrosis ◽

Nutritional Deficiencies ◽

Transformation Rate ◽

Areca Nut ◽

Healthy Individuals ◽

Submucous Fibrosis ◽

Hematological Abnormalities

Background. Oral submucous fibrosis is a chronic, insidious oral mucosal condition affecting the most parts of the oral cavity with high malignant transformation rate triggered by areca nut chewing, nutritional deficiencies, immunologic processes, and genetic predisposition. OSF causes significant hematological abnormalities resulting in anemia and a decrease in serum iron levels.Aim. The aim of this study was to estimate the hemoglobin and serum iron levels among patients with oral submucous fibrosis and to compare the values with healthy subjects.Materials and Methods. In this hospital-based study 30 diagnosed patients of OSMF and 15 healthy individuals were included, and the values of hemoglobin and serum iron levels were estimated using Sahli’s and Ferrene methods.Results. OSMF patients showed significantly lower levels of hemoglobin and serum iron when compared with the healthy subjects.Conclusion. The findings of the study emphasizes on the assessment of hemoglobin and serum iron for patients with oral submucous fibrosis. Also iron therapy should be instituted concomitantly with the initial diagnosis which helps to cease the further progression of the condition. Further extensive studies are indicated to understand the correlation between OSMF and iron deficiency.

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