Analysis of Copper and Zinc Levels in the Mucosal Tissue and Serum of Oral Submucous Fibrosis Patients

2010 ◽  
Vol 1 (2) ◽  
pp. 75-78 ◽  
Author(s):  
Ajay G Nayak ◽  
Laxmikanth Chatra ◽  
K Prashanth Shenai
Keyword(s):  
Trace Elements ◽  
Oral Mucosa ◽  
Oral Submucous Fibrosis ◽  
Zinc Level ◽  
Copper Level ◽  
Pathogenetic Mechanisms ◽  
Copper And Zinc ◽  
Submucous Fibrosis ◽  
The Mean ◽  
Zinc Levels

ABSTRACT Background and Objectives Oral submucous fibrosis (OSMF) is a well-recognized potentially malignant disease of oral mucosa whose exact pathogenetic mechanisms have not been understood. Measurement of copper and zinc levels in the tissue and serum of these patients may be helpful in understanding the pathogenesis. Therefore, a study was undertaken to analyze the levels of these trace elements in the populations of the coastal region of south Karnataka and north Kerala, India, who show predominant use of areca nut due to their cultural and social backgrounds. Methods A case-control study was conducted on 20 cases of OSMF and 20 controls. The tissue and serum concentrations of copper and zinc in these 40 subjects were measured by colorimetry. Results The mean tissue copper level in OSMF group was 4.31 ± 1.13 μg/gm, while the tissue zinc level was 25.18 ± 4.92 μg/gm. The mean serum copper level in the OSMF group was 1.00 ± 0.20 μg/ml while the mean serum zinc level was 0.92 ± 0.18 μg/ml. Interpretation and conclusion The tissue copper levels in OSMF group showed very highly significant difference (p = 0.001) from the controls while the difference in tissue zinc levels was highly significant (p = 0.009). The serum levels of copper (p = 0.35) and zinc (p = 0.08) did not exhibit any statistical difference from those of the control group. These findings indicate that the trace elements, copper and zinc, within the oral mucosa definitely, have a role to play in the pathogenetic mechanisms of OSMF.

scholarly journals Estimation of Serum Copper and Zinc Levels in Oral Submucous Fibrosis: An Atomic Absorption Spectroscopic Study

2013 ◽  
Vol 14 (5) ◽  
pp. 801-805 ◽  
Author(s):  
H Neethi
Keyword(s):  
Spectroscopic Study ◽  
Atomic Absorption ◽  
Oral Submucous Fibrosis ◽  
Serum Copper ◽  
Stage I ◽  
Healthy Controls ◽  
Copper And Zinc ◽  
Submucous Fibrosis ◽  
The Mean ◽  
Zinc Levels

ABSTRACT Background and objectives Biochemical derangements of microelements although reported in oral cancer is poorly understood in oral submucous fibrosis (OSMF). Hence, the present study was carried out to estimate and compare the serum copper and zinc levels among different histopathological stages of OSMF with that of healthy controls. Materials and methods Thirty histopathologically diagnosed cases of OSMF and 30 healthy controls reporting to MS Ramaiah Dental College and Hospital (MSRDCH), Bengaluru, were included in the study. The histopathological staging of OSMF was done as per criteria given by Pindborg and Sirsat (1966). Blood samples were collected and the serum copper and zinc levels were estimated using atomic absorption spectroscopy. The results were statistically analyzed using independent t-test and Kruskal-Wallis test. Results The mean serum copper levels were increased (p < 0.0005) and zinc levels were decreased (p < 0.01) in OSMF patients when compared to that of controls. The mean serum copper levels showed an increasing trend from stage I to IV (p = 0.004) while zinc levels showed a decreasing trend from stage I to IV (p = 0.04) in OSMF patients. Conclusion The serum copper and zinc levels in OSMF showed a significant difference in comparison to that of controls and between the histopathological stages. These findings indicate that serum copper and zinc may have a contributory role in the etiopathogenesis of OSMF. Clinical relevance Although the role of the trace elements as etiological factors is minor, derangements noted in the serum copper and zinc levels could be correlated with diseased progression and possibly explain the transformation of OSMF into malignancy. Thus, they can be used as prognostic markers and can be of value for proactive intervention. How to cite this article Neethi H, Patil S, Rao RS. Estimation of Serum Copper and Zinc Levels in Oral Submucous Fibrosis: An Atomic Absorption Spectroscopic Study. J Contemp Dent Pract 2013;14(5):801-805.

Serum Copper and Zinc Levels of Oral Submucous Fibrosis Patients Using Areca Nut Products Reported at Isra University Hospital Hyderabad

2021 ◽  
Vol 15 (9) ◽  
pp. 3034-3036
Author(s):  
Uzma Tariq ◽  
Waqas Iqbal ◽  
Surwaich Ali Channa ◽  
Bhunesha Devi ◽  
Arhama Surwaich ◽  
...  
Keyword(s):  
Oral Submucous Fibrosis ◽  
Serum Zinc ◽  
Serum Copper ◽  
University Hospital ◽  
Areca Nut ◽  
Healthy Individuals ◽  
Copper And Zinc ◽  
Submucous Fibrosis ◽  
Zinc Levels ◽  
One Year

Objective: To determine the copper and zinc levels in serum among oral submucous fibrosis patients using various areca nut products by taking healthy individuals as control at Isra University Hospital Hyderabad. Methodology: This descriptive case-control study was conducted on patients of oral submucosal fibrosis (OSMF) attending the Outpatient Department, Oral Surgery Isra Dental College, Isra university during a period of one year from November 2016 to October 2017. A total of 30 patients above 16 years of age and who were diagnosed cases of OSMF and gave history of areca nut and its commercial products consumption for more than one year were included as group (B)and in equal number healthy individuals were enrolled as control as group(A). The 6ml blood sample was collected in gel tube and then we centrifuged at 3000 rpm gravity for 10min to separate the serum from blood. The 4 to 5ml serum collected in this way was analyzed by spectrophotometric method on Hitachi 902(Roche Germany). The levels of copper and zinc were estimated from serum of both groups. All the data was collected via study proforma and analysis of the data was done by using SPSS version 20. Results: Mean age of the patients was 23.33±4.97 years and mean age of healthy controls was 22.80±4.61 years. The males were most common in both groups. Manipuri was commonest consumed areca nut (50.0%) followed by sweet scented areca nut and Gutkha 33.3% and 36.6% respectively. Mean of serum Cu in patients was lower 86±4.20 µg/dl in contrast to controls 114.0±13.8 µg/dl (p-0.0001). Average of serum Zinc was higher among patients 94.20±15.11 µg/dl as compared to controls as 68.06±17.10 µg/dl (p-0.0001). Conclusion: The average of serum copper level was observed to be significantly less and average of serum zinc significantly higher among OSMF patients using areca nut as compared to healthy individuals. These serum trace element levels could be used as potential prognostic and diagnostic markers in the OSMF patients. Key words: OSMF, Zinc, Copper, Areca nut

scholarly journals Analysis of Serum and Urinal Copper and Zinc in Chinese Northeast Population with the Prediabetes or Diabetes with and without Complications

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Jiancheng Xu ◽  
Qi Zhou ◽  
Gilbert Liu ◽  
Yi Tan ◽  
Lu Cai
Keyword(s):  
Serum Zinc ◽  
Zinc Level ◽  
Serum Copper ◽  
Copper Level ◽  
Simvastatin Treatment ◽  
Copper And Zinc ◽  
Urinary Copper ◽  
Zinc Levels

This study investigated the association of copper and zinc levels in the serum or urine of patients living in northeast China, with either prediabetes or diabetes. From January 2010 to October 2011, patients with type 1 diabetes (T1D,n=25), type 2 diabetes (T2D,n=137), impaired fasting glucose (IFG,n=12) or impaired glucose tolerance (IGT,n=15), and age/gender matched controls (n=50) were enrolled. In the T2D group, there were 24 patients with nephropathy, 34 with retinopathy, and 50 with peripheral neuropathy. Serum copper levels were significantly higher in IFG, IGT, and T2D groups. Serum zinc level was dramatically lower, and urinary zinc level was significantly higher in both T1D and T2D subjects compared with controls. The serum zinc/copper ratio was significantly lower in all the patients with IFG, ITG, T1D, and T2D. The serum copper level was positively associated with HbA1c in T2D subjects. Simvastatin treatment in T2D patients had no significant effect on serum and urinary copper and zinc. These results suggest the need for further studies of the potential impact of the imbalanced serum copper and zinc levels on metabolic syndrome, diabetes, and diabetic complications.

Utilization of lupin stubbles by merino sheep: studies of animal performance, rates and time of stocking, lupinosis, liver copper and zinc, and circulating plasma enzymes

1979 ◽  
Vol 30 (3) ◽  
pp. 551 ◽  
Author(s):  
KP Croker ◽  
JG Allen ◽  
DS Petterson ◽  
HG Masters ◽  
RF Frayne
Keyword(s):  
Liver Damage ◽  
Significant Negative Correlation ◽  
Zinc Level ◽  
Copper Level ◽  
Liver Copper ◽  
Plasma Enzymes ◽  
Copper And Zinc ◽  
Phomopsis Leptostromiformis ◽  
Zinc Levels ◽  
Stocking Rates

Three experiments were conducted in the West Midland region of Western Australia to evaluate the sheep-carrying capacity of dry narrow-leafed sweet lupin (Lupinus angustifolius cv. Uniharvest) stubbles. In addition, the effect of stocking rate on lupinosis, and the effect of lupinosis on circulating plasma enzymes and liver copper and zinc levels were determined. At low stocking rates (25 or less ha-1) sheep were grazed for up to 80 days with increases in liveweight. However, with higher stocking rates (50 or 75 ha-1) the maximum weights obtained were lower, and reached after a shorter period of time. Sheep grazed at the high stocking rates suffered more liver damage than those grazed at low rates, and it is suggested that outbreaks of clinical lupinosis might be precipitated by grazing sheep at high rates. Sheep that grazed the stubbles and suffered liver damage had higher liver copper levels than those that suffered no damage. Also, there was an indication that the degree of liver damage and the liver copper level were directly related. It is suggested that the toxins of Phomopsis leptostromiformis cause the liver to take up and store copper. A significant negative correlation between the degree of liver damage and the liver zinc level was found. However, it was not determined whether this diminishment of liver zinc was a direct effect of the toxins of P. leptostromiformis or due to displacement by copper which was actively being taken up. Plasma levels of ornithine carbamyl transferase, alkaline phosphatase and aspartate amino transferase, measured during or at the end of the experiments, were not related to the degree of terminal liver injury. They are not considered reliable indicators of the incidence or severity of lupinosis in the field.

scholarly journals Comparative Study of Intralesional Dexamethasone Plus Hyaluronidase & Oral Colchicine in Patients with Oral Submucous Fibrosis

2018 ◽  
Vol 14 (2) ◽  
pp. 60-65
Author(s):  
Ganesh Prasad Neupane ◽  
Maya Rai ◽  
R. S. Rathore ◽  
V. K. Bhargava ◽  
A. K. Mahat ◽  
...  
Keyword(s):  
Treatment Outcomes ◽  
Oral Mucosa ◽  
Oral Submucous Fibrosis ◽  
Mouth Opening ◽  
Study Groups ◽  
T Test ◽  
Significant Difference ◽  
Intralesional Injections ◽  
Submucous Fibrosis ◽  
Larger Sample

Introduction: Oral Submucous Fibrosis (OSMF) is a precancerous condition of the oral mucosa. It is characterized by excessive production of collagen leading to inelasticity of the oral mucosa and atrophic changes of the epithelium.Aim and objective: To evaluate the efficacy of oral Colchicine in comparison to intralesional injections of Dexamethasone plus Hyaluronidase in the management of OSMF patients.Materials and Methods: Fourty patients with OSMF were randomly divided equally into two groups. 20 patients in Dexamethasone group received biweekly intralesional injections of Dexamethasone (4mg/ml) plus Hyaluronidase 1500 IU in buccal mucosa for a period of 12 weeks. Other 20 patients in Colchicine group received oral Colchicine 0.5 mg tablets twice daily for 12 weeks. Parameters taken in the study were burning sensation, and mouth opening. Descriptive statistics, paired t test and unpaired t test were used for statistical analysis.Results and Conclusions: The pre- and post-treatment differences were found to be statistically significant for both the groups (p<0.001) and for both the treatment outcomes. When the average difference of the treatment outcomes was compared between the two study groups, statistically highly significant difference was noted (p <0.001) only in mouth opening but not in burning sensation.These encouraging results should prompt further clinical trials with Colchicine on a larger sample size to broaden the therapeutic usefulness of the drug in the management of OSMF. JNGMC,  Vol. 14 No. 2 December 2016, Page: 60-65

scholarly journals Regulation of Collagen Synthesis in Tobacco/Pan-Masala Induced Oral Sub-Mucosal Fibrosis and Diagnostic Approach - Review Article

2021 ◽  
pp. 134-143
Author(s):  
Shraddha Awasthi ◽  
Ausaf Ahmad ◽  
Anand Narain Srivastava
Keyword(s):  
Oral Mucosa ◽  
Collagen Synthesis ◽  
Oral Submucous Fibrosis ◽  
Malignant Lesion ◽  
Life Quality ◽  
Premalignant Lesions ◽  
Reference List ◽  
Transformation Rate ◽  
Dental Services ◽  
Submucous Fibrosis

<p><strong>Introduction :</strong> oral submucosal fibrosis (OSMF) is related to juxta epithelial inflammatory response followed by a fibroelastic change of the lamina propria, with epithelial atrophy leading to stiffness of the oral mucosa, causing trismus and inability to eat.</p> <p><strong>Methods and Materials:</strong> The suitability standards for studies were: Research Articles that studied the metabolic activities in oral submucous fibrosis mainly caused by some carcinogenic substances or chemicals which may present in tobacco, alcohol, cigarettes, pan-masala etc Reference list of selected articles and reviews on the topic also were searched. studies were extracted, A total of 90-100 studies were extracted, using the keywords oral submucous fibrosis, oral potentially malignant lesion and diagnosis, collagen synthesis etc.,</p> <p><strong>Results: </strong>cancer risk among these patients, periodic biopsies of suspicious regions of the oral mucosa are essential for the early detection and management of high?risk oral premalignant lesions and prevention of cancer.</p> <p><strong>Conclusion</strong>: OSMF is hardly recognized and is poorly understood across the globe. significant improvement in management, nor reduction in its high malignant transformation rate. Better integration of medical and dental services, especially in developing countries, may reduce patients’ suffering and improve their life quality.</p>

Study of trace elements (copper & zinc) in oral submucous fibrosis

1987 ◽  
Vol 39 (3) ◽  
pp. 104-106
Author(s):  
R. P. Gupta ◽  
K. Rai ◽  
D. D. Hemani ◽  
A. K. Gupta
Keyword(s):  
Trace Elements ◽  
Oral Submucous Fibrosis ◽  
Submucous Fibrosis ◽  
Copper Zinc

scholarly journals Studies on the Hematological Profile and Trace Elements in Oral Submucous Fibrosis.

1995 ◽  
Vol 19 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Cunnigaiper Dhanasekaran ANURADHA ◽  
Chennam Srinivasulu SHYAMALA DEVI
Keyword(s):  
Trace Elements ◽  
Oral Submucous Fibrosis ◽  
Hematological Profile ◽  
Submucous Fibrosis

scholarly journals Hearing Efficiency in Oral Submucous Fibrosis: A Clinical Study

2020 ◽  
Author(s):  
J. S. Shah ◽  
Nutan Lunagariya
Keyword(s):  
Oral Mucosa ◽  
Conductive Hearing Loss ◽  
Oral Submucous Fibrosis ◽  
Hearing Threshold ◽  
Mouth Opening ◽  
Burning Sensation ◽  
Areca Nut ◽  
Gradual Reduction ◽  
Submucous Fibrosis ◽  
Conductive Hearing

Abstract Oral Submucous fibrosis (OSMF) is a chronic insidious disease of oral mucosa that occurs due to areca-nut chewing, consumption of chillies, autoimmunity and genetic predisposition. The disease starts with burning sensation and inability to tolerate spicy foods with gradual reduction in mouth opening due to fibrosis of the oral mucosa. The extension of fibrosis into the naso pharynx leads to reduction in hearing efficiency. As very few studies had been done to evaluate the hearing disability in OSMF patients, this study had been undertaken to prove the same. To evaluate hearing efficiency in patients with Oral Submucous Fibrosis of various grades of severity. Presentation includes 30 patients of osmf with various grades and evaluated for hearing efficacy by audiometry. Hearing threshold was compared in different grades of osmf. The present study revealed a significant association between OSMF and hearing deficit. Involvement of the palatal muscles with OSMF may decrease the patency of the Eustachian tube, leading to conductive hearing loss. Therefore, the protocol for managing OSMF patients should include ENT consultation and treatment for hearing deficit in order to increase the success rate of treatment.

scholarly journals Serum levels of matrix metalloproteinase-2 in patients with oral submucous fibrosis and oral squamous cell carcinoma

2020 ◽  
Vol 8 (2) ◽  
pp. 636
Author(s):  
Pooja Kherde ◽  
Suchitra Gosavi
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Matrix Metalloproteinase ◽  
Healthy Subjects ◽  
Oral Submucous Fibrosis ◽  
Positive Association ◽  
Serum Levels ◽  
Matrix Metalloproteinase 2 ◽  
Submucous Fibrosis ◽  
The Mean

Background: Matrix metalloproteinase-2 (MMP-2), which is supposed to enable cancer cells cross the basement membrane and metastasize by selectively cleaving type IV collagen, is anticipated to be a good diagnostic and prognostic marker in oral squamous cell carcinoma (OSCC) and oral submucous fibrosis (OSMF). Thus, the study is aimed to estimate and compare the serum MMP-2 levels in patients with OSMF and OSCC.Methods: The study was conducted on 88 subjects, divided into three groups; Group I (healthy subjects, n=28), Group II (patients with OSMF, n=30), and Group III (patients with OSCC, n=30). Serum levels of MMP-2 were estimated and compared among the groups and further with the clinical parameters within the groups.Results: The mean serum MMP-2 levels in patients with OSMF (2.87±1.04 ng/mL) and with OSCC (11.55±2.16 ng/mL) were significantly higher than the healthy subjects (0.93±0.26 ng/mL) (p <0.0001, for both). Furthermore, the mean serum MMP-2 levels in OSMF subjects had a positive association with inter-incisal opening (IIO), however, there was no association with the degree of burning sensation. Likewise, in subjects with OSCC, levels of serum MMP-2 showed positive association with histopathological grades, however, significant association with the site of occurrence and primary tumor size was not found.Conclusions: Elevated serum MMP-2 levels can be used as a screening tool in the early detection of OSMF and OSCC cases. Moreover, MMP-2 might be a good marker in evaluating the tumor grade in OSCC and the IIO in OSMF.

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