scholarly journals Efek Pemberian Kotoran Ayam Dikombinasikan Dengan Ampas Tahu Terhadap Peningkatan Kualitas Pertumbuhan Daphnia. sp

2017 ◽  
Vol 3 (1) ◽  
pp. 1-14
Author(s):  
Ma'watun Hasanah ◽  
Armen Nainggolan ◽  
Firsty Rahmatia

The cultivation of Daphnia magna usually uses fertilization method, both organic and inorganic fertilization. The cultivation of Daphnia magna. Usually done by using chicken manure as a culture medium to grow microorganisms as feed Daphnia magna. In addition to efforts to grow microorganisms as a source of nutrients for Daphnia magna, it is necessary additional protein intake is useful for the population of microorganisms. Protein sources obtained from Tofu dregs. Objectives to be achieved from the implementation of this research are: 1). Know the effect of dosage of manure (chicken manure) with tofu tofu as feed for population growth of Daphnia magna. 2). Knowing the population growth rate of Daphnia magna. 3). Knowing the quality of Daphnia magna as a natural food of fish seed. The research method was chicken dung manure combined with dregs of tofu (22.5 g / l tofu, 7.5 gr / l of chicken manure), (15 g / l tofu, 15 g / l of manure Chickens), 7.5 gr / l tofu, 22.5 g / l of chicken manure), (30 g / l of chicken manure), 30 gr / l tofu ) and without chicken manure and tofu dregs. The results showed that population growth, growth rate and peak population were significantly different between the treatment of chicken manure and tofu dregs. While long growth there is no significant difference between the treatment of chicken manure and tofu dregs.

PELAGICUS ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 45
Author(s):  
Hernika Simanjuntak ◽  
Ernik Yuliana ◽  
Sinar Pagi Sektiana

ABSTRAKDaphnia magna memiliki banyak keunggulan sebagai pakan alami pada budidaya ikan fase larva. Tujuan penelitian ini adalah membandingkan pertumbuhan Daphnia magna pada budidaya dengan menggunakan beberapa sumber air pupuk/pakan. Penelitian ini dilakukan secara eksperimental dengan metode Rancangan Acak Lengkap (RAL) dengan perlakuan yaitu pemberian pupuk pakan: kotoran ayam, air cucian beras, dan air rebusan kedelai, masing-masing pada konsentrasi 2%, 5% dan 10% dengan 3 kali pengulangan. Analisis data yang digunakan adalah uji statistik ANOVA dan dilanjutkan dengan uji beda nyata terkecil (BNT). Hasil penelitian menunjukkan bahwa pertumbuhan populasi yang lebih baik adalah dengan penambahan pakan/pupuk air rebusan kedelai dengan konsentrasi berturut-turut 10%, 5%, dan 2%. Hasil uji ANOVA menunjukkan perbedaan yang siginifikan antar perlakuan, namun tidak berbeda signifikan pada penggunaan konsentrasi yang berbeda. Hasil uji BNT menunjukkan bahwa air rebusan kedelai konsentrasi 5% dan 10% memberikan respon yang lebih baik dengan rata-rata pertumbuhan populasi 346 ekor dan 534 ekor. Hasil pengujian kandungan protein dan lemak didapatkan hasil bahwa air rebusan kedelai lebih tinggi dibanding yang lainnya yaitu rata-rata sebesar 2,50% dan 5,77%. Pengujian kandungan Escherichia coli didapatkan hasil jika semua perlakuan menunjukkan kandungan E. coli yang negatif.ABSTRACTDaphnia magna has many advantages as natural food in larval stage fish culture. This study aims to compare the growth of Daphnia magna in cultivation using several sources of fertilizer/feed water. This research was conducted experimentally with a completely randomized design method with the treatment of feeding fertilizer: chicken manure, rice washing water, and soy boiled water, each at a concentration of 2%, 5% and 10% with 3 repetitions. The data analysis used was the ANOVA statistical test and continued with the least significant difference test (LSD). The results indicated that the better population growth was the addition of soybean boiled feed/water fertilizer with concentrations of 10%, 5%, and 2%, respectively. The results of the ANOVA test indicated that was a significant difference between treatments, but not significantly different at the use of different concentrations. LSD test results indicated that soybean boiled water with a concentration of 5% and 10% gave a better response with an average population growth of 346 and 534 individuals. The results of testing the protein and fat content showed that the cooking water for soybeans was higher than the others, namely 2.50% and 5.77%, respectively. The test for Escherichia coli content was obtained if all treatments showed negative E. coli content.


Author(s):  
Thulani R. Kunene ◽  
Michael T. Masarirambi ◽  
Paul K. Wahome ◽  
Tajudeen O. Oseni

The excessive unjustified use of some kinds of fertilizers has seen some farmers realizing poor quality fruit that does not appeal to the final consumer, thus negatively affecting the effort of alleviating poverty in the Kingdom of Eswatini. This experiment was carried out at the Horticulture Department Lath House, Faculty of Agriculture, Luyengo Campus of the University of Eswatini to determine the growth, yield and shelf-life of green pepper when fertilized with kraal manure, poultry manure and inorganic fertilizer. The experiment was conducted to find the optimum levels of fertilizers that promotes the growth of pepper and to find the effects of different fertilizers on yield and quality of pepper. The treatments included kraal manure applied at 60 t/ha, [NPK (2:3:2) 37] at 370 kg/ha, chicken manure at 40 t per hectare and the control with no amendment. The results showed that growing pepper using the four treatments significantly affected its growth rate, leaf number, fruit number and its (fruit) shelf life. Pepper grown using inorganic fertilizer had the highest leaf number followed in decreasing order by chicken manure, kraal manure and lastly peppers which did not receive any amendment.  There were no significant difference in the growth rate of pepper in the inorganic fertilizer and chicken manure treatments. Similarly, there was no significant difference of pepper grown with chicken manure and chemical fertilizer in the number of days it took the harvested pepper to reach 100% decay stored at room temperature for 21 days. Yet pepper grown with kraal manure was significantly different from the two as it showed 20% decay rate in the same number of days. Although the control had the least decay, the yield was the lowest thus it is not recommended. Kraal manure at 60 t/ha is recommended in the production of pepper with a longer shelf life.


Zygote ◽  
2019 ◽  
Vol 27 (02) ◽  
pp. 78-81
Author(s):  
R. Rodríguez Díaz ◽  
R. Blanes Zamora ◽  
R. Vaca Sánchez ◽  
J. González Pérez ◽  
J.C. Alberto Bethencourt

SummaryHLA-G expression has been detected in early preimplantation embryos and it has been postulated that a relationship between embryonic expression of this factor and successful pregnancy may exist. Forty-six patients were prospectively selected from our centre ‘Unidad de Reproducción Humana, Hospital Universitario de Canarias’ for conducting this study. In all cases, metaphase II (MII) oocytes were fertilized using intracytoplasmic sperm injection 2–4 h after retrieval. Embryos were cultured individually in 20 µl droplets of G-1 medium (VitroLife) under oil at 37°C and a 6% CO2environment. Fertilization was assessed at 18 h postinsemination and all oocytes fertilized were passed into a new culture plaque individually in 300 µl culture medium until day 3 of culture. The culture medium was examined for the expression and secretion of sHLA-G with a sandwich enzyme-linked immunosorbent assay kit (BioVendor, Heidelberg, Germany) according to the manufacturer’s instructions. We found statistical significance between higher levels of sHLA-G secretion and pregnancy rate. When both groups were compared there was no difference in embryo quality of transferred embryos, but a significant difference in the number of oocytes and the embryo quality of the cohort existed that was greater in the pregnant group. A standardized sHLA-G assay with a specifically defined range and standard units provides a non-invasive method to identify the most competent embryos for transfer.


1993 ◽  
Vol 56 (1) ◽  
pp. 115-119 ◽  
Author(s):  
N. K. Waran ◽  
D. M. Broom

AbstractWhen designing environments for animals, the quality of space as well as the amount of space should be considered. In this study the influence of an opaque barrier on the aggressive behaviour and growth of Large White Χ Landrace piglets that were weaned at 24 days was examined. The behaviour and weight gain of 120 piglets were recorded during a 4-week period following weaning. These piglets were housed in either a conventional flat-deck pen or a straw pen, with or without an opaque barrier. There was no significant difference between the two types of accommodation in the frequency of aggressive interactions that occurred during the weaning period but where a barrier was present the frequency of aggressive interactions was 40% lower during the 1st week after weaning and growth rate was proportionately 0·15 greater. Piglets that were the recipients of most aggressive behaviour used the barrier most frequently during the weaning period and barrier users gained the most weight during the week immediately following weaning.It was concluded that a barrier improved the weaning environment because it offered piglets an escape route during the period when most aggressive interactions occurred. A barrier may be an effective way of improving the welfare of animals housed in confined conditions.


2018 ◽  
Vol 66 (4) ◽  
pp. 518-529 ◽  
Author(s):  
Mahek Anand ◽  
Bence Lázár ◽  
Roland Tóth ◽  
Emőke Páll ◽  
Eszter Patakiné Várkonyi ◽  
...  

Primordial germ cells (PGCs) were isolated from blood samples of chicken embryos. We established four PGC lines: two males (FS-ZZ-101, GFP-ZZ-4ZP) and two females (FS-ZW-111, GFP-ZW-5ZP). We could not detect a significant difference in the marker expression profile, but there was a remarkable difference between the proliferation rates of these PGC lines. We monitored the number of PGCs throughout a three-day period using a high-content screening cell imaging and analysing system (HCS). We compared three different initial cell concentrations in the wells: ~1000 cells (1×, ~4000 (4× and ~8000 (8×. For the GFPZW- 5ZP, FS-ZZ-101 and FS-ZW-111 PGC lines the lowest doubling time was observed at 4× concentration, while for GFP-ZZ-4ZP we found the lowest doubling time at 1× concentration. At 8× initial concentration, the growth rate was high during the first two days for all cell lines, but this was followed by the appearance of cell aggregates decreasing the cell growth rate. We could conclude that the difference in proliferation rate could mainly be attributed to genotypic variation in the established PGC lines, but external factors such as cell concentration and quality of the culture medium also affect the growth rate of PGCs.


2020 ◽  
Vol 7 (1) ◽  
pp. 33-49
Author(s):  
Pienyani Rosawanti ◽  
Nurul Hidayati ◽  
Fahruddin Arfianto ◽  
Djoko Eko Hadi Susilo

This research aims to know the effect interaction of application of types and different dosage of organic fertilizers on production and fruit quality of melons on peatlands, effect of application of types and different dosage of organic fertilizers on production and fruit quality of melons on peatlands, and the agronomic efficiency of the use of several types and different dosage of organic fertilizers on production of melons on peatlands. This research design was used as a Randomized Block Design (RBD) consists of two factors with 4 replication. The first factor consisted of types of organic fertilizer (P) i.e. chicken manure (PA), guano manure (PG), and cow manure (PS). The second factor consisted of fertilizer dosage (D) i.e. 0 tons ha-1 (without fertilization, called control), D1 = 15 tons ha-1, D2 = 30 tons ha-1 and D3 = 45 tons ha-1. The data obtained by analysis of variance (ANOVA) or F test at a = 5% and 1% levels to determine the effect of treatment was tested further by HSD (honestly significant difference) testat the level of 5%. The result shows that the interaction type of organic fertilizer and the dosage of organic fertilizer treatment significant effect on the weight of the melon. Chicken manure is the best organic fertilizer on the size and quality of the melon. Dosage of 45 tons ha-1 organic fertilizer gave the highest results but it was not significantly different with a dosage of 30 tons ha-1. The use of chicken manure fertilizer of 30 tons ha-1 is more efficient in agronomy in increasing melons yield on peatlands.


2021 ◽  
Author(s):  
Larissa Andrade-Vieira ◽  
Clement Bojic ◽  
Ingrid Alvarenga ◽  
Teotonio de Carvalho ◽  
Jean-François Masfaraud ◽  
...  

Abstract Dimethyl sulfoxide (DMSO) is widely used as a vehicle solvent in ecotoxicity bioassays. However, despite its frequent use, itcould be toxic for organisms at some concentrations. Hence, the aim of this study was to investigate the effectsof DMSO on the population growth rate of the microalgaeRaphidocelis subcapitata, the mobility of the microcrustacean Daphnia magna,and the reproduction of the rotiferBrachionus calyciflorus. DMSO was applied to the organisms in concentrations ranging from 0.031–4%. For R. subcapitata significant effects in growth inhibitionafter 72 h of exposure was 0.125% DMSO,being the lowest observed effectconcentration (LOEC). The 50% effective concentration (EC50) was2.138 ± 0.372%. In D. magna,significant differences in the mobility after 24 h or 48 h of exposure was 1% DMSO being 1.712± 0.207% and 1.167± 0.220%DMSO the EC50 observed for 24 h and 48 h exposure, respectively. For B. calyciflorus,it was not possible to validate the tests performed, as there were insufficient animals alive in the control conditions at the end of the exposure period. Therefore, we recommended avoiding DMSO as a vehicle in assays using B. calyciflorus,and to use final DMSO concentrationsin experimental solution not exceeding 0.125% forR. subcapitata and 0.5% for D. magna.


1982 ◽  
Vol 45 (4) ◽  
pp. 356-359 ◽  
Author(s):  
G. F. IBRAHIM

Batches of pasteurized milk inoculated with spores of Clostridium sporogenes, C. perfringens, C. butyricum and C. tyrobutyricum were used to produce 8 batches of Cheddar cheese without starter activity. At the end of cheddaring, half the curd was salted and each curd portion was pressed separately for 18 h at ambient temperature. The cheese was stored for 6 weeks at both 11 and 4 C then processed. Samples of each batch of processed cheese were maintained at room temperature and at 37 C for at least 10 months. The results showed that although the salted cheese (SC) contained 5% salt-in-moisture and less moisture content than that of the unsalted cheese (USC), no appreciable difference was observed in growth rate of clostridia in both USC and SC. After processing, there was no significant difference between spore counts in the cheese originating from either USC or SC. No defects which could be attributed to clostridial activity developed during storage. Browning of processed cheese stored at 37 C was far greater in batches originating from SC as compared with those originating from USC. Results of this investigation were compatible with results published previously regarding omission of salting of low acid Cheddar cheese with the object of retarding Staphylococcus aureus growth and enterotoxin production.


2005 ◽  
Vol 24 (9) ◽  
pp. 2254 ◽  
Author(s):  
Barry J. Pieters ◽  
Albrecht Paschke ◽  
Sebastián Reynaldi ◽  
Michiel H.S. Kraak ◽  
Wim Admiraal ◽  
...  

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