EVALUATION OF ANTIBACTERIAL POTENTIAL OF EXTRACTS OF MOCHARAS (GUM OF BOMBAX MALABARICUM) AGAINST CERTAIN BACTERIAL STRAINS

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (09) ◽  
pp. 45-52
Author(s):  
Akhtar Sada ◽  
Abdur Rauf ◽  
Sumbul Rehman ◽  
Mohd Zakir Siddiqui
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
Gram Positive ◽  
Standard Drug ◽  
Gram Negative ◽  
The Family ◽  
Antibacterial Potential

Mocharas is the gum of the tree Bombax malabaricum DC which belongs to the family Bombaceae. In Unani system of medicine, it is used in the treatment of various infectious diseases. In the present study, various extracts of Mocharas (B. malabaricum) were tested for their antibacterial activity against some gram positive (Streptococcus mutans, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Corynebacterium xerosis and Bacillus cereus) and gram negative (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Proteus vulgaris) bacterial strains using Agar Well method and Kirby Bauer’s disc diffusion method. The potency of the extracts to microorganisms was compared with the standard drug, Ciprofloxacin (SD060) 5µg/disk for gram positive bacterial strains and Gentamicin (SD016) 10µg/disk for gram negative bacterial strains. Results were interpreted on the basis of Zone of Inhibition (ZOI), Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Hydroalcoholic and alcoholic extracts showed significant antibacterial activity than aqueous extract.

scholarly journals Antibacterial activity of Beta vulgaris L. pomace extract

2011 ◽  
pp. 263-269 ◽  
Author(s):  
Aleksandra Velicanski ◽  
Dragoljub Cvetkovic ◽  
Sinisa Markov ◽  
Jelena Vulic ◽  
Sonja Djilas
Keyword(s):  
Antibacterial Activity ◽  
Beta Vulgaris ◽  
Staphylococcus Epidermidis ◽  
Control Sample ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Natural Isolates

Antibacterial activity of Beta vulgaris L. (beetroot) pomace extract (concentration 100 mg/ml) was tested against five Gram positive and seven Gram negative bacterial strains (reference cultures and natural isolates). Disc diffusion method with 15 ?l of extract and agar-well diffusion method with 50 and 100 ?l were used. Antibiotic (cefotaxime/clavulanic acid) was used as a control sample. The tested extract showed the highest activity against Staphylococcus aureus and Bacillus cereus, where clear zones (without growth) appeared. There was no any activity against other tested Gram-positive bacteria, except for Staphylococcus epidermidis, with a small zone of reduced growth. Growth of all tested Gram-negative bacteria was inhibited usually with 100 ?l of extract. The most susceptible were Citrobacter freundii and Salmonella typhymurium. The tested antibiotic gave clear, usually large zones for all tested strains except for Staphylococcus cohni spp. cohni, where only a zone of reduced growth appeared.

scholarly journals Antibacterial Potential of Aloe weloensis (Aloeacea) Leaf Latex against Gram-Positive and Gram-Negative Bacteria Strains

2019 ◽  
Vol 2019 ◽  
pp. 1-4
Author(s):  
Yohannes Kelifa Emiru ◽  
Ebrahim Abdela Siraj ◽  
Tekleab Teka Teklehaimanot ◽  
Gedefaw Getnet Amare
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Maximum Activity ◽  
New Drugs ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative

Objective. To evaluate the antibacterial effects of the leaf latex of Aloe weloensis against infectious bacterial strains. Methods. The leaf latex of A. weloensis at different concentrations (400, 500, and 600 mg/ml) was evaluated for antibacterial activities using the disc diffusion method against some Gram-negative species such as Escherichia coli (ATCC 14700) and Pseudomonas aeruginosa (ATCC 35619) and Gram-positive such as Staphylococcus aureus (ATCC 50080) and Enterococcus fecalis (ATCC 4623). Results. The tested concentrations of the latex ranging between 400 and 600 mg·mL−1 showed significant antibacterial activity against bacterial strain. The highest dose (600 mg/ml) of A. weloensis leaf latex revealed the maximum activity (25.93 ± 0.066 inhibition zone) followed by the dose 500 mg/ml against S. aureus. The lowest antibacterial activity was observed by the concentration 400 mg/ml (5.03 ± 0.03) against E. coli. Conclusion. The results of the present investigation suggest that the leaf latex of A. weloensis can be used as potential leads to discover new drugs to control some bacterial infections.

scholarly journals Evaluation of antibacterial activity of vitamin C against human bacterial pathogens

2023 ◽  
Vol 83 ◽  
Author(s):  
S. Mumtaz ◽  
S. Mumtaz ◽  
S. Ali ◽  
H. M. Tahir ◽  
S. A. R. Kazmi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Vitamin C ◽  
Bacterial Infections ◽  
Unmet Need ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative ◽  
Adjunct Treatment

Abstract Now a day’s multidrug resistance phenomenon has become the main cause for concern and there has been an inadequate achievement in the development of novel antibiotics to treat the bacterial infections. Therefore, there is an unmet need to search for novel adjuvant. Vitamin C is one such promising adjuvant. The present study was aimed to elucidate the antibacterial effect of vitamin C at various temperatures (4°C, 37°C and 50°C) and pH (3, 8, and 11), against Gram-positive and Gram-negative bacteria at various concentrations (5-20 mg/ml) through agar well diffusion method. Growth inhibition of all bacterial strains by vitamin C was concentration-dependent. Vitamin C significantly inhibited the growth of Gram-positive bacteria: Bacillus licheniformis (25.3 ± 0.9 mm), Staphylococcus aureus (22.0 ± 0.6 mm), Bacillus subtilis (19.3 ± 0.3 mm) and Gram-negative bacteria: Proteus mirabilis (27.67 ± 0.882 mm), Klebsiella pneumoniae (21.33±0.9 mm), Pseudomonas aeruginosa (18.0 ± 1.5 mm) and Escherichia coli (18.3 ± 0.3 mm). The stability of vitamin C was observed at various pH values and various temperatures. Vitamin C showed significant antibacterial activity at acidic pH against all bacterial strains. Vitamin C remained the stable at different temperatures. It was concluded that vitamin C is an effective and safe antibacterial agent that can be used in the future as an adjunct treatment option to combat infections in humans.

scholarly journals Comparative Study of Antibacterial Activity of Juice and Peel Extract of Citrus Fruits

2019 ◽  
Vol 6 ◽  
pp. 82-88 ◽  
Author(s):  
Alisha Shakya ◽  
Bhawana Luitel ◽  
Pragati Kumari ◽  
Ritu Devkota ◽  
Puspa Raj Dahal ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Diffusion Method ◽  
Citrus Limon ◽  
Citrus Fruits ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
Gram Positive ◽  
E Coli ◽  
Bacillus Spp ◽  
Peel Extract

Objectives: This study was aimed to compare the antibacterial activity of peel and juice extracts of citrus fruits. Methods: The antibacterial activity of juice and peel extract of five selected citrus fruits [C1- Citrus reticulata (mandarin orange), C2- Citrus limetta (sweet lime), C3- Citrus aurantifolia (lime), C4- Citrus limon (lemon) and C5- Citrus maxima (pommelo)] on three Gram positive and five Gram negative bacterial strains were examined. The juice and peel extract of citrus fruits was obtained by using absolute ethanol. The obtained extracts were dissolved in 1ml 10% v/v DMSO which was taken to carry out the antibacterial susceptibility assay using agar well diffusion method. Results: The peel extracts of C1 and C2 were found to be more effective against the given Gram-positive bacteria than the juice extracts with maximum zone of inhibition (20.33±1.527) and (15.33±0.577) against Bacillus spp respectively. While the juice extracts of C3 and C4 were more effective against both Gram positive and negative bacteria than their peel extracts with maximum zone of inhibition (26.66±1.15) against S. aureus ATCC 25923 and (20±1) against E. coli ATCC 25922 respectively. The juice extract of C5 was found to be more effective against both Gram positive and negative bacteria than its peel extract with maximum zone of inhibition (18.66±1.154) against E. coli ATCC 25922 except for Bacillus spp in which peel extract showed maximum zone of inhibition i.e. (15±1) Conclusion: This study suggests that these juice and peel extracts may have beneficial antibacterial roles that can be exploited in controlling unwanted bacterial growth.

scholarly journals SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION OF SUBSTITUTED 1,3,4-OXADIAZOLE DERIVATIVE: DERIVED FROM CIPROFLOXACIN

2019 ◽  
pp. 205-209
Author(s):  
ANUJ SINGHAI ◽  
M.K. GUPTA
Keyword(s):  
Antibacterial Activity ◽  
Biological Evaluation ◽  
Diffusion Method ◽  
Gram Positive ◽  
Standard Drug ◽  
Gram Negative ◽  
Oxadiazole Derivatives ◽  
Gram Negative Organisms ◽  
Derivatives Of

Objective: The purpose of this research is synthesized and evaluates different derivatives of oxadiazole. Methods: A novel series of substituted 1,3,4-oxadiazole derivative were synthesized by condensing different amine with 1-cyclopropyl-6-fluoro-7- (piperazin-1-yl)-3-(5-thioxo-4,5-dihydro-1,3,4-oxadiazol-2-yl)quinolin-4(1H)-one (III) in the presence of formaldehyde. The structure of these novel synthesized compounds was characterized on the bases of physicochemical and spectral analysis. The title compounds (IVa-h) were screened for antibacterial activity by disc diffusion method. Results: Substituted 1,3,4-oxadiazole derivative was synthesized, characterized, and evaluated for antibacterial activity. Compounds IVa, IVd, IVe, IVf, and IV h showed enhance activities then ciprofloxacin against all Gram-positive and Gram-negative organisms. Compound IVe showed the highest activity against Staphylococcus aureus and compound IV showed the highest activity against Escherichia coli. Conclusion: The present study demonstrates the synthesis and characterization of 1,3,4-oxadiazole derivatives derived from ciprofloxacin. These compounds were evaluated for antibacterial activity against different Gram-positive and Gram-negative organism. In some cases, antibacterial activity is found to be enhanced as compared to standard drug ciprofloxacin.

scholarly journals In-vitro analysis of potential antibacterial activity of three medicinal plants

2016 ◽  
Vol 8 (3) ◽  
pp. 1497-1500
Author(s):  
Vandana Gupta ◽  
Rakesh Kumar ◽  
Deepika Chaudhary ◽  
Nirmal Yadav
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Medicinal Plants ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria

The present study was aimed to examine and compare the antibacterial activity of hot methanolic extract of medicinal plants viz. Portulaca oleracea (purslane), Syzygium cumini (L.) (jamun), Psidium guajava (L.) (guava). Antibacterial activity was carried by using agar well diffusion method, against Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and Gram-negative bacteria (Escherichia coli). Results indicated that all the three plant extracts possess antibacterial property against Gram-positive bacteria and no activity was found against Gram-negative bacteria. Moderate zone of inhibition against Staphylococcus aureus and Bacillus subtilis was exhibited by S. cumini (L.) (11mm and 12mm) and P. guajava (L.) (10mm and 11mm) and weak zone of inhibition was exhibited by P. oleracea (5 mm and 6mm). In conclusion, S. cumini (L.) and P. guajava (L.) possess bettercapabilities of being a good candidate in search for natural antibacterial agent against infections and diseases causing Gram-positive bacteria as compared to P. oleracea.

STUDIES ON THE ANTIBACTERIAL ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF ROSA INDICA L.

2021 ◽  
Vol 1 (5) ◽  
pp. 60-69
Author(s):  
Gandhi maniyan ◽  
Ranjith C
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Petroleum Ether Extract ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Gram Positive ◽  
Vibrio Cholera ◽  
Gram Negative

Medicinal plants are extensively used in traditional medicine to cure various infectious diseases in human. Plant based natural constituents can be derived from any part of the plant like bark, leaves, flowers, roots, fruits, seeds, etc. Medicinal plants are commonly used in the cure of diseases since times immemorial. In the present study methanol, chloroform and aqueous extracts of Rosa indica L. leaf were subjected for antimicrobial activity by well-diffusion method in the case of evaluate the antibacterial activity of Petroleum ether extract of Rosa indica L.extract against gram-positive cultures include B. cereus, B. subtilis. Gram-negative cultures include Vibrio cholera, Klebsiella pneumonia. The maximum antibacterial activity against Bacillus cereus (Zone of inhibition in mm 14.2), B. subtilis (zone of inhibition 13.1) and athanolic the lowest activity against Vibrio cholera (Zone of inhibition 12.6), Klebsiella pneumonia (Zone of inhibition 12.3). The gram positive bacteria more susceptible than the gram negative bacteria. The phytochemical characteristics of Rosa indica L. plants tested were summarized in the, it could be seen that, carbohydrates, cardiac glycosids, Flavonoids, Saponins (Foam test) Amino acids , Terpenoids, Phenols and Tannins were present in Rose indica plant extract.

scholarly journals PYRAZOLINE SYNTHESIS THROUGH A CHALCONE INTERMEDIATE

2018 ◽  
Vol 2 (4) ◽  
pp. 59-62
Author(s):  
Md Hedaitullah ◽  
Ramanpreet Walia ◽  
Iqbal Khalid ◽  
Balwan Singh ◽  
Asif Hussain
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Drug Research ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Pharmacological Activities ◽  
Gram Positive ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Antibacterial Potential

Pyrazoline derivatives, being used as potential medicinal agents, possess many important pharmacological activities and therefore they are useful materials in drug research. A series of these derivatives HT- 1 to HT- 6 containing  5-4-(chlorophenyl)-1,3-diphenyl- 4,5-dihydro-1H-pyrazole derivatives were synthesized, structures were confirmed using melting point, IR, NMR and MS and evaluated for their antibacterial activity using disc diffusion method at concentration 2mg/ml. The reference used was Amikacin and mostly, all synthesized compounds exhibited a significant antibacterial activity against Staphylococcus aureus (MTCC No. 96) as Gram positive bacteria, Staphylococcus epidermidis(MTCC No. 435) as Gram positive and Escherichia coli (MTCC No. 739) as Gram negative bacteria. It was observed that HT-1 and HT-2 possess good antibacterial potential against S. aureus, HT- 5 against S.epidermidis and HT-1 and HT-4against E. coli.

scholarly journals Antimicrobial Activity of Inga fendleriana Extracts and Isolated Flavonoids

2009 ◽  
Vol 4 (12) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Luisa Pistelli ◽  
Alessandra Bertoli ◽  
Cecilia Noccioli ◽  
Jeanette Mendez ◽  
Rosa Anna Musmanno ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Kinetic Studies ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative ◽  
Bactericidal Effects ◽  
Time Kill

The EtOAc and n-BuOH extracts of Inga fendleriana inhibited Gram-positive, but not Gram-negative bacteria; a narrow spectrum of activity against Staphylococcus epidermidis was detected. The MIC values of the extracts ranged from 125 to 850 μg/mL. Quercetin 3-methylether, myricetin 3-O-rhamnoside and tricetin showed antibacterial activity against the same bacterial strains with MICs in the range from 31 to 250 μg/mL. In time-kill kinetic studies, the flavonoids showed bactericidal effects at the concentrations corresponding to four times the MICs.

In-vitro Antioxidant and Antibacterial Activity of Methanolic Extract of Shorea robusta Gaertn. F. Resin

2016 ◽  
Vol 6 (4) ◽  
pp. 68
Author(s):  
Sushma Vashisht ◽  
Manish Pal Singh ◽  
Viney Chawla
Keyword(s):  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Gram Positive ◽  
Gram Negative ◽  
Shorea Robusta ◽  
Dose Dependent ◽  
Resin Extract

The methanolic extract of the resin of Shorea robusta was subjected to investigate its antioxidant and antibacterial properties its utility in free radical mediated diseases including diabetic, cardiovascular, cancer etc. The methanol extract of the resin was tested for antioxidant activity using scavenging activity of DPPH (1,1-diphenyl-2-picrylhydrazil) radical method, reducing power by FeCl3 and antibacterial activity against gram positive and gram negative bacteria using disc diffusion method. The phytochemical screening considered the presence of triterpenoids, tannins and flavoniods. Overall, the plant extract is a source of natural antioxidants which might be helpful in preventing the progress of various oxidative stress mediated diseases including aging. The half inhibition concentration (IC50) of resin extract of Shorea robusta and ascorbic acid were 35.60 µg/ml and 31.91 µg/ml respectively. The resin extract exhibit a significant dose dependent inhibition of DPPH activity. Antibacterial activity was observed against gram positive and gram negative bacteria in dose dependent manner.Key Words: Shorea robusta, antioxidant, antibacterial, Disc-diffusion, DPPH.

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