scholarly journals Growth promotion and yield enhancement of barley cultivars using ACC deaminase producing Pseudomonas fluorescens strains under salt stress

2019 ◽  
Vol 17 (1) ◽  
pp. e0801 ◽  
Author(s):  
Mitra Azadikhah ◽  
Fatemeh Jamali ◽  
Hamid-Reza Nooryazdan ◽  
Fereshteh Bayat
Keyword(s):  
Salt Stress ◽  
Plant Growth ◽  
Salinity Stress ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Growth And Yield ◽  
Salt Tolerant ◽  
Barley Cultivars ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth-promoting rhizobacteria containing 1-aminocyclopropane-1-carboxylate (ACC) deaminase enzyme reduce the level of stress, ethylene and stimulate plant growth under various biotic and abiotic stress conditions. The present study aims at characterizing efficient salt-tolerant, ACC deaminase containing Pseudomonas fluorescens strains with plant growth-promoting activity isolated from the rhizosphere of barley plants and evaluating the influence of potent plant growth-promoting rhizobacteria (PGPR) isolates on growth and yield of five barley cultivars under salinity stress. Plant growth and yield in barley cultivars following inoculation with salt-tolerant, ACC deaminase producing PGPR strains under salt stress were quantified. Results indicated that under various levels of salinity (50, 100 and 150 mM NaCl) inoculation with PGPRs had positive impact on growth parameters and yield of barley cultivars including plant height, spike length, weight and number, peduncle length, number of grains per spike, 1000-grain weight and grain yield, comparing to uninoculated control plants under salinity stress. Inoculation of barley cultivars with bacteria ameliorated the negative effects of salinity and resulted in increase in growth and yield. Besides, as the salinity levels increased, growth and yield of barley cultivars decreased; however, cultivars showed different responses to salt stress. This study demonstrates the vital role of rhizobacteria containing ACC deaminase for increasing salt tolerance and consequently improving the growth and yield of barley plants under salinity stress.

Rhizobacteria containing ACC-deaminase confer salt tolerance in maize grown on salt-affected fields

2009 ◽  
Vol 55 (11) ◽  
pp. 1302-1309 ◽  
Author(s):  
Sajid Mahmood Nadeem ◽  
Zahir Ahmad Zahir ◽  
Muhammad Naveed ◽  
Muhammad Arshad
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Plant Growth ◽  
Ethylene Production ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Growth And Yield ◽  
Negative Effect ◽  
Plant Growth Promoting ◽  
Growth Promoting

Salt stress is one of the major constraints hampering agricultural production owing to its impact on ethylene production and nutritional imbalance. A check on the accelerated ethylene production in plants could be helpful in minimizing the negative effect of salt stress on plant growth and development. Four Pseudomonas , 1 Flavobacterium , and 1 Enterobacter strain of plant growth promoting rhizobacteria containing 1-aminocyclopropane-1-carboxylate (ACC)-deaminase were selected and their effects on growth and yield of maize were investigated to improve the salt tolerance of maize grown on salt-affected fields. The selected rhizobacterial isolates reduced or eliminated the classical “triple” response, indicating their ability to reduce stress-induced ethylene levels. Results showed that rhizobacterial strains, particularly Pseudomonas and Enterobacter spp., significantly promoted the growth and yield of maize compared with the non-inoculated control. Pseudomonas fluorescens increased plant height, biomass, cob yield, grain yield, 1000 grain mass, and straw yield of maize up to 29%, 127%, 67%, 60%, 17%, and 166%, respectively, over the control. Under stress conditions, more N, P, and K uptake and high K+–Na+ ratios were recorded in inoculated plants compared with the control. The results imply that inoculation with plant growth promoting rhizobacteria containing ACC-deaminase could be a useful approach for improving growth and yield of maize under salt-stressed conditions.

Isolation and identification of salt-tolerant plant-growth-promoting rhizobacteria and their application for rice cultivation under salt stress

2020 ◽  
Vol 66 (2) ◽  
pp. 144-160 ◽  
Author(s):  
Shahnaz Sultana ◽  
Sumonta C. Paul ◽  
Samia Parveen ◽  
Saiful Alam ◽  
Naziza Rahman ◽  
...  
Keyword(s):  
Salt Stress ◽  
Plant Growth ◽  
Oryza Sativa L ◽  
Plant Growth Promoting Rhizobacteria ◽  
Salt Resistance ◽  
Salt Tolerant ◽  
Isolation And Identification ◽  
Enhanced Expression ◽  
Plant Growth Promoting ◽  
Growth Promoting

Growth and productivity of rice are negatively affected by soil salinity. However, some salt-tolerant rhizosphere-inhabiting bacteria can improve salt resistance of plants, thereby augmenting plant growth and production. Here, we isolated a total of 53 plant-growth-promoting rhizobacteria (PGPR) from saline and non-saline areas in Bangladesh where electrical conductivity was measured as >7.45 and <1.80 dS/m, respectively. Bacteria isolated from saline areas were able to grow in a salt concentration of up to 2.60 mol/L, contrary to the isolates collected from non-saline areas that did not survive beyond 854 mmol/L. Among the salt-tolerant isolates, Bacillus aryabhattai, Achromobacter denitrificans, and Ochrobactrum intermedium, identified by comparing respective sequences of 16S rRNA using the NCBI GenBank, exhibited a higher amount of atmospheric nitrogen fixation, phosphate solubilization, and indoleacetic acid production at 200 mmol/L salt stress. Salt-tolerant isolates exhibited greater resistance to heavy metals and antibiotics, which could be due to the production of an exopolysaccharide layer outside the cell surface. Oryza sativa L. fertilized with B. aryabhattai MS3 and grown under 200 mmol/L salt stress was found to be favoured by enhanced expression of a set of at least four salt-responsive plant genes: BZ8, SOS1, GIG, and NHX1. Fertilization of rice with osmoprotectant-producing PGPR, therefore, could be a climate-change-preparedness strategy for coastal agriculture.

scholarly journals Plant Growth-promoting Rhizobacteria Mitigate Deleterious Effects of Salt Stress on Strawberry Plants (Fragaria ×ananassa)

HortScience ◽  
2013 ◽  
Vol 48 (5) ◽  
pp. 563-567 ◽  
Author(s):  
Huseyin Karlidag ◽  
Ertan Yildirim ◽  
Metin Turan ◽  
Mucahit Pehluvan ◽  
Figen Donmez
Keyword(s):  
Salt Stress ◽  
Plant Growth ◽  
Chlorophyll Content ◽  
Field Experiments ◽  
Plant Growth Promoting Rhizobacteria ◽  
Element Content ◽  
Growth And Yield ◽  
Nutrient Element ◽  
Plant Growth Promoting ◽  
Growth Promoting

The effect of selected plant growth-promoting rhizobacteria (PGPR) on the growth, chlorophyll content, nutrient element content, and yield of strawberry plants under natural field salinity conditions stress was investigated. Field experiments were conducted using a randomized complete block design with five PGPRs (Bacillus subtilis EY2, Bacillus atrophaeus EY6, Bacillus spharicus GC subgroup B EY30, Staphylococcus kloosii EY37, and Kocuria erythromyxa EY43) and a control (no PGPR) in 2009 and 2010. PGPR inoculations significantly increased the growth, chlorophyll content, nutrient element content, and yield of strawberry plants. PGPR treatments lowered electrolyte leakage of plants under saline conditions. The leaf relative water content (LRWC) of plants rose with bacterial inoculation. All nutrient element contents of leaves and roots investigated were significantly increased with PGPR inoculations with the exception of sodium (Na) and chlorine (Cl). The highest efficiency to alleviate salinity stress on the yield and nutrient uptake of strawberry plants was obtained from EY43 (228 g per plant) and EY37 (225 g per plant) treatment and the yield increasing ratio of plants was 48% for EY43 and 46% for EY 37 compared with the control treatment (154 g per plant). The highest nitrogen (N), potassium (K), phosphorus (P), calcium (Ca), magnesium (Mg), sulfur (S), manganese (Mn), copper (Cu), and iron (Fe) concentrations were obtained from EY43 and followed by E6, E37, and E30, and increasing ratio of leaves and root N, P, K, Ca, Mg, S, Mn, Cu, and Fe contents were 22% to 33%, 34% to 8.8%, 89% to 11%, 11.0% to 7.2%, 5.1% to 6.2%, 97% to 65%, 120% to 140%, 300% to 15%, and 111% to 9.0%, respectively. The results of the study suggested that PGPR inoculations could alleviate the deleterious effects of salt stress conditions on the growth and yield of strawberry plants under salinity conditions.

scholarly journals USE OF PLANT GROWTH PROMOTING RHIZOBACTERIA AGAINST SALT STRESS FOR TOMATO (SOLANUM LYCOPERSICUM L.) SEEDLING GROWTH

2020 ◽  
Vol 19 (6) ◽  
pp. 15-29
Author(s):  
Yagmur Yilmaz ◽  
Ceknas Erdinc ◽  
Ahmet Akkopru ◽  
Selma Kipcak
Keyword(s):  
Salt Stress ◽  
Plant Growth ◽  
Pseudomonas Putida ◽  
Photosynthetic Pigment ◽  
Plant Growth Promoting Rhizobacteria ◽  
Growth And Yield ◽  
Plant Tolerance ◽  
Membrane Injury ◽  
Plant Growth Promoting ◽  
Growth Promoting

Salt stress affects many aspects of plant metabolism and as a result, growth and yield are reduced. The aim in this study was to determine the effects of plant growth promoting rhizobacteria (PGPR) on tomato plants under salt stress. With this aim, the Interland F1 cv. and bacterial isolates of Bacillus thuringiensis CA41/1, Pseudomonas putida 18/1K, Pseudomonas putida S5/4ep, and Pseudomonas putida 30 were used. Salt application was completed in two different doses of 25 and 50 mM NaCl when seedlings reached the stage of 3 true leaves. At the end of the study, in addition to seedling development criteria, some nutrient element contents and rates (K, Ca, Na, K/Na and Ca/Na), superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) enzyme activities, malondialdehyde (MDA) and photosynthetic pigment contents were determined. In the stress environment, PGPR inoculation increased K content by up to 10%, while apart from isolate P. putida no.30, the other isolates lowered Na content by up to 18%. Additionally, 18/1K and S5/4ep isolates were identified to reduce membrane injury index by up to 97%. It was identified that CA41/1, 18/1K and S5/4ep isolates were more effective against salt stress, especially. In general, the plant tolerance levels induced by the bacteria were identified to increase with the increase in salt stress.

scholarly journals Plant Growth Promoting Rhizobacterial Mitigation of Drought Stress in Crop Plants: Implications for Sustainable Agriculture

Agronomy ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 712 ◽  
Author(s):  
Omena Ojuederie ◽  
Oluwaseyi Olanrewaju ◽  
Olubukola Babalola
Keyword(s):  
Drought Stress ◽  
Plant Growth ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Crop Growth ◽  
Growth And Yield ◽  
Growth Enhancement ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Effects Of Drought

Abiotic stresses arising from climate change negates crop growth and yield, leading to food insecurity. Drought causes oxidative stress on plants, arising from excessive production of reactive oxygen species (ROS) due to inadequate CO2, which disrupts the photosynthetic machinery of plants. The use of conventional methods for the development of drought-tolerant crops is time-consuming, and the full adoption of modern biotechnology for crop enhancement is still regarded with prudence. Plant growth-promoting rhizobacteria (PGPR) could be used as an inexpensive and environmentally friendly approach for enhancing crop growth under environmental stress. The various direct and indirect mechanisms used for plant growth enhancement by PGPR were discussed. Synthesis of 1-aminocyclopropane−1-carboxylate (ACC) deaminase enhances plant nutrient uptake by breaking down plant ACC, thereby preventing ethylene accumulation, and enable plants to tolerate water stress. The exopolysaccharides produced also improves the ability of the soil to withhold water. PGPR enhances osmolyte production, which is effective in reducing the detrimental effects of ROS. Multifaceted PGPRs are potential candidates for biofertilizer production to lessen the detrimental effects of drought stress on crops cultivated in arid regions. This review proffered ways of augmenting their efficacy as bio-inoculants under field conditions and highlighted future prospects for sustainable agricultural productivity.

scholarly journals Plant Growth-Promoting Rhizobacteria Enhance Salinity Stress Tolerance in Okra through ROS-Scavenging Enzymes

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Sheikh Hasna Habib ◽  
Hossain Kausar ◽  
Halimi Mohd Saud
Keyword(s):  
Plant Growth ◽  
Stress Tolerance ◽  
Salinity Stress ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Antioxidant Enzyme Activities ◽  
Ros Scavenging ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Salinity Stress Tolerance

Salinity is a major environmental stress that limits crop production worldwide. In this study, we characterized plant growth-promoting rhizobacteria (PGPR) containing 1-aminocyclopropane-1-carboxylate (ACC) deaminase and examined their effect on salinity stress tolerance in okra through the induction of ROS-scavenging enzyme activity. PGPR inoculated okra plants exhibited higher germination percentage, growth parameters, and chlorophyll content than control plants. Increased antioxidant enzyme activities (SOD, APX, and CAT) and upregulation of ROS pathway genes (CAT, APX, GR, and DHAR) were observed in PGPR inoculated okra plants under salinity stress. With some exceptions, inoculation withEnterobactersp. UPMR18 had a significant influence on all tested parameters under salt stress, as compared to other treatments. Thus, the ACC deaminase-containing PGPR isolateEnterobactersp. UPMR18 could be an effective bioresource for enhancing salt tolerance and growth of okra plants under salinity stress.

scholarly journals Bacillus tequilensis strain ‘UPMRB9’ improves biochemical attributes and nutrient accumulation in different rice varieties under salinity stress

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0260869
Author(s):  
Rakiba Shultana ◽  
Ali Tan Kee Zuan ◽  
Mohd Rafii Yusop ◽  
Halimi Mohd Saud ◽  
Ahmed M. El-Shehawi
Keyword(s):  
Plant Growth ◽  
Salinity Stress ◽  
Plant Growth Promoting Rhizobacteria ◽  
Salt Tolerant ◽  
Rice Varieties ◽  
Rice Plants ◽  
Tolerant Plant ◽  
Bacillus Tequilensis ◽  
Plant Growth Promoting ◽  
Growth Promoting

Soil salinity exert negative impacts on agricultural production and regarded as a crucial issue in global wetland rice production (Oryza sativa L.). Indigenous salt-tolerant plant growth-promoting rhizobacteria (Bacillus sp.) could be used for improving rice productivity under salinity stress. This study screened potential salt-tolerant plant growth-promoting rhizobacteria (PGPR) collected from coastal salt-affected rice cultivation areas under laboratory and glasshouse conditions. Furthermore, the impacts of these PGPRs were tested on biochemical attributes and nutrient contents in various rice varieties under salt stress. The two most promising PGPR strains, i.e., ‘UPMRB9’ (Bacillus tequilensis 10b) and ‘UPMRE6’ (Bacillus aryabhattai B8W22) were selected for glasshouse trial. Results indicated that ‘UPMRB9’ improved osmoprotectant properties, i.e., proline and total soluble sugar (TSS), antioxidant enzymes like superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT). Moreover, ‘UPMRB9’ inoculated rice plants accumulated higher amount of nitrogen and calcium in tissues. Therefore, the indigenous salt-tolerant PGPR strain ‘UPMRB9’ could be used as a potential bio-augmentor for improving biochemical attributes and nutrient uptake in rice plants under salinity stress. This study could serve as a preliminary basis for future large-scale trials under glasshouse and field conditions.

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