scholarly journals Food additives reduce lactic acid bacterial growth in culture medium and in meat products, increasing product shelf life

2015 ◽  
Vol 36 (6) ◽  
pp. 3681
Author(s):  
Cleonice Mendes Pereira Sarmento ◽  
Eliane Colla ◽  
Cristiane Canan ◽  
Francieli Dalcanton ◽  
Gláucia Maria Falcão de Aragão
Keyword(s):  
Lactic Acid ◽  
Sodium Chloride ◽  
Shelf Life ◽  
Bacterial Growth ◽  
Culture Medium ◽  
Food Additives ◽  
Meat Products ◽  
Sodium Lactate ◽  
Lag Phase ◽  
Pork Sausages

The uncontrolled growth of lactic acid bacteria (LAB) in meat and meat products leads to product spoilage, and thus shortens product shelf life. Although food additives are known to decrease LAB growth, this effect has not been analyzed in detail. Here, a detailed analysis was performed of the effects of sodium chloride, sodium polyphosphate, sodium lactate, sodium nitrite/nitrate, and garlic on the growth of the Lactobacillus plantarum in culture medium. The results were used to design and test experimental formulations of meat products. Initially, the effect of food additives on L. plantarum was evaluated using a Fractional Factorial Design (FFD), followed by a Central Composite Rotatable Design (CCRD). The Modified Gompertz Model was adjusted to the growth curves to determine the Kinetic parameters of bacterial growth (logarithmic increase in the population, specific growth rate, and lag phase extension). Higher sodium lactate and sodium chloride levels had a negative impact on L. plantarum growth parameters (p?0.05). Therefore, we designed experimental formulations of mortadella and smoked pork sausages containing 4% sodium lactate (w w-1) and 2.4-3.5% sodium chloride (w w-1), and determined LAB growth from samples of stored products produced according to these formulations, in order to determine product shelf life. There was an increased lag phase of LAB growth for most experimental formulations. Also, the experimental smoked pork sausages had a longer shelf life, which was increased by at least 22 days, suggesting that the proposed formulation, with higher than standard lactate concentration, increased the product’s shelf life.

Evaluation of Sodium Diacetate and ALTA™ 2341 on Viability of Listeria monocytogenes in Turkey Slurries

1993 ◽  
Vol 56 (9) ◽  
pp. 808-810 ◽  
Author(s):  
JIMMY H. SCHLYTER ◽  
ALAN J. DEGNAN ◽  
JODI LOEFFELHOLZ ◽  
KATHLEEN A. GLASS ◽  
JOHN B. LUCHANSKY
Keyword(s):  
Listeria Monocytogenes ◽  
Shelf Life ◽  
Generation Time ◽  
Food Additives ◽  
Lag Phase ◽  
Breast Meat ◽  
Turkey Meat ◽  
Log10 Unit ◽  
Sodium Diacetate

The antilisterial activity of sodium diacetate and a commercial shelf-life extender (ALTA™ 2341) were monitored at 25°C in slurries prepared with turkey breast meat. In slurries prepared without either ingredient, populations of Listeria monocytogenes increased about 5-log10 units in 7 d. The addition of 0.3% diacetate extended the generation time (7 h) compared to the control (no food additives; 1.7 h), whereas 0.5% inhibited the pathogen somewhat (0.4-log10 unit decrease in 7 d compared to the control). Slurries containing ALTA (0.25, 0.5, or 0.75%) and 0.3% diacetate extended the lag phase of L. monocytogenes to a greater extent than slurries with 0.3% diacetate alone. In contrast, 0.5% diacetate in combination with all three levels of ALTA tested was listericidal (ca. 2-log10 unit decrease after 7 d compared to the control). These data confirm the efficacy of diacetate for inhibiting L. monocytogenes in turkey meat and indicate that multiple barriers such as diacetate with ALTA may further lessen the likelihood of food-related listeriosis.

Sodium Lactate Addition on the Quality and Shelf Life of Refrigerated Sliced Poultry Sausage Packaged in Air or Nitrogen Atmosphere

2004 ◽  
Vol 67 (3) ◽  
pp. 601-606 ◽  
Author(s):  
RENATA CEGIELSKA-RADZIEJEWSKA ◽  
JAN PIKUL
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Shelf Life ◽  
Sodium Lactate ◽  
Life Extension ◽  
Poultry Meat ◽  
Color Measurement ◽  
Refrigerated Storage ◽  
Psychrotrophic Bacteria

The aim of this study was to determine the effect of sodium lactate addition on shelf-life extension of sliced poultry sausage packaged both in air and nitrogen atmospheres and stored in refrigerated conditions. Basic chemical composition, pH, and malonaldehyde content were assayed and color measurement using the reflection method was carried out. Microbiological examination consisted of determination of total number of aerobic psychrotrophic bacteria and number of lactic acid bacteria. Sensory evaluation of products was performed. Microbiological and sensory quality of sliced poultry meat sausage was dependent on the addition during production of sodium lactate and the composition of gases (air or nitrogen) used in packaging. Slices of poultry sausage with 1% as well as 2% of sodium lactate maintained their initial quality of evaluated sensory attributes longer, irrespective of the applied gases. Sodium lactate inhibited growth of aerobic psychrotrophic bacteria and lactic acid bacteria during refrigerated storage. Sodium lactate also inhibited the formation of malonaldehyde in sliced poultry sausage during refrigerated storage. The effectiveness of this process depended on the concentration of sodium lactate addition. It was concluded that 1% as well as 2% addition of sodium lactate could extend the shelf life of sliced poultry sausage packaged in air atmosphere and stored at 5 to 7°C by 3 or 4 times, respectively. Sliced poultry sausage treated with 2% sodium lactate packed in nitrogen had the longest (35-day) shelf life. This was a sevenfold increase in the shelf life of sliced poultry sausage compared with the control.

Acid Tolerance and Acid Shock Response of Escherichia coli O157:H7 and Non-O157:H7 Isolates Provide Cross Protection to Sodium Lactate and Sodium Chloride

1998 ◽  
Vol 61 (2) ◽  
pp. 158-161 ◽  
Author(s):  
DONNA MAE GARREN ◽  
MARK A. HARRISON ◽  
SCOTT M. RUSSELL
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Sodium Chloride ◽  
Acid Tolerance ◽  
Sodium Lactate ◽  
Cross Protection ◽  
Escherichia Coli O157 ◽  
Strain Atcc ◽  
Acid Shock ◽  
Shock Response

The survival of Escherichia coli O157:H7 and non-O157:H7 due to an enhanced acid tolerance response (ATR), an enhanced acid shock response (ASR), or the stationary phase protective system when exposed to lactic acid and the resulting cross protection against increased concentrations of sodium chloride and sodium lactate was studied. Escherichia coli O157:H7 isolates (932 and E009) and a non-O157:H7 strain (ATCC 23716) were grown to stationary phase at 32°C and exposed to one of two treatments in an attempt to either acid shock or acid adapt the survivors. Acid-shocked cells were exposed to lactic acid at pH 4.0. Acid-adapted cells were first exposed to a pH of 5.5 and then an acid challenge of pH 4.0. Sodium lactate (10%, 20%, or 30%) or sodium chloride (5%, 10%, or 15%) were added to a minimal glucose medium after the acidification treatments. When acid shocked and acid adapted isolate 932 and strain ATCC 23716 tolerated the elevated levels of sodium lactate, and the strain ATCC 23716 tolerated the elevated levels of sodium chloride. Acid adaption allowed isolate 932 to tolerate higher levels of sodium chloride; however, the acid shocking did not provide the same protection. Neither of the acid treatments provided increased tolerance to sodium chloride for isolate E009. Evidence of cross protection against acid and sodium chloride or acid and sodium lactate in E. coli O157:H7 could point to a need for further evaluation of whether these combinations of preservation means are sufficient to control this pathogen.

scholarly journals INNOVATIVE DECISIONS TO IMPROVE FOOD QUALITY AND SAFETY

Food systems ◽  
2019 ◽  
Vol 2 (4) ◽  
pp. 14-17 ◽  
Author(s):  
V. V. Eveleva ◽  
T. M. Cherpalova
Keyword(s):  
Lactic Acid ◽  
Shelf Life ◽  
Mass Fraction ◽  
Food Additives ◽  
Food Products ◽  
Optimal Level ◽  
Food Additive ◽  
Life Extension ◽  
Wheat Bread ◽  
Included Trial

The scope of the project consisted in research-based development of new complex food additives from lactic acid and its derivatives for enhancing microbiological safety and shelf life extension of healthy food products. The object of research included: trial samples of complex food additives from lactic acids and its derivatives. The samples were obtained chemically using the following basic components: food grade lactic acid with 79.6% base substance mass fraction; acetic acid with 99.8% base substance mass fraction; propionic acid with 99.6% base substance mass fraction; neutralizing agents for synthesis of salts of the acids used, and propylene glycol with 99.8% base substance mass fraction. The optimal balance of the ingredients in the formula of the additive delivers the optimal level of true acidity combined with antimicrobial and antioxidant effect, and enhances organoleptical performance and process parameters of food products. This complex food additive containing lactate and acetate features high counter-regulatory effect on testing cultures of pathogenic organisms of rope spoilage of wheat bread and can be used for production of non-perishable products. The use of complex food additive in the production of dressed fish preserves activates biochemical processes related to fish maturation and delivers improved product quality and extended shelf life.

scholarly journals ANALISIS VIABILITAS LACTOBACILLUS LACTIS PADA INOVASI MEDIA DASAR PERTUMBUHAN ALTERNATIF DAN MEDIA DASAR PENEPUNGAN BAKTERI ASAM LAKTAT

2020 ◽  
Vol 4 (2) ◽  
pp. 16-22
Author(s):  
Ani Sulastri ◽  
Baso Manguntungi
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Shelf Life ◽  
Bacterial Growth ◽  
Alternative Media ◽  
Plate Count ◽  
Growth Media ◽  
Media Formulation ◽  
The Media ◽  
Lactobacillus Lactis

The limited shelf life in a food requires a natural preservative so that the food used is not easily damaged and has a longer shelf life, namely by using lactic acid bacteria (BAL) using alternative media. By using lactic acid bacteria, the time in the storage period food products can be extended. The purpose of this study was to determine the viability of the Lactobacillus lactis bacteria on an alternative growth base media and a media on the media of bacteria. Lactic acid bacteria were rejuvenated and culture propagation of 5 ?l was inoculated into 5 mL of MRSB media. Formulation media used for bacterial growth such as whey tofu + 5% sucrose + 1% urea. The alternative media was incubated for 24 hours. Bacterial growth was observed at 0, 4, 8 and 16 hours using the TPC (Total Plate count) method. Various media Lactobacillus lactis bacterial deposition was grown on MRSB media and dried with freeze dry for 48 hours and the viability of Lactobacillus lactis was tested. The basic growth media that can be used are Lactobacillus lactis bacteria, namely whey tofu + sucrose 5% + urea 1% as well as Lactobacillus lactis viability results in various media which are grown on MRS media and various alternative media shows that the media has a 100% carrageenan composition able to maintain the viability of Lactobacillus lactis cells.

scholarly journals Effect of propionic and lactic acids on in vitro ruminal bacteria growth

2011 ◽  
Vol 40 (5) ◽  
pp. 1121-1127 ◽  
Author(s):  
Juliana Silva de Oliveira ◽  
Augusto César de Queiroz ◽  
Hilário Cuquetto Mantovani ◽  
Marcelo Rodrigues de Melo ◽  
Edenio Detmann ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Propionic Acid ◽  
Culture Medium ◽  
Lag Phase ◽  
Ruminal Bacteria ◽  
Bacteria Growth ◽  
Ruminal Microorganisms ◽  
The Media ◽  
Lactic Acids

The objective of this work was to evaluate the effect of the levels of lactic and propionic acids on in vitro fermentation of ruminal microorganisms. In experiment 1, the levels, in a total of 12 were the following: addition of 0 (control 1), 50, 100, 150, 200 and 250 mM of lactic acid and 0 (control 2), 50, 100, 150, 200 and 250 mM of propionic acid, respectively, in incubation flasks, which contained ruminal inoculum, glucose and synthetic culture medium, with two repetitions for each combination. In experiment 2, the combinations, in a total of 4, were the following: presence of 12 and 24 mM of propionic acid and 0 mg of glucose, respectively; presence of 12 and 24 mM of propionic acid and 40 mg of glucose, respectively, to the incubation flasks which contained ruminal inoculum, with or without glucose and in synthetic culture medium with two repetitions each. There was no effect on the specific growth velocity of ruminal microorganisms in the presence of lactic acid or propionic acid. However, when there were greater concentrations of these acids in the media, there was a longer lag phase in the microorganism phase. Acid propionic at the concentration of 24 mM inhibited the production of acid acetic and butyric acid in a media with glucose. Despite of not being used as a source of energy by the ruminal microorganisms, propionic acid affects their metabolism. Lactic and propionic acids inhibit growth of some ruminal microorganisms at elevated concentrations.

Bacterial growth in refrigerated, vacuum-packed luncheon meats

1970 ◽  
Vol 16 (5) ◽  
pp. 287-297 ◽  
Author(s):  
A. G. Kempton ◽  
S. R. Bobier
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Shelf Life ◽  
Bacterial Growth ◽  
Small Proportion ◽  
Growth Curves ◽  
Selective Medium ◽  
Refrigerated Storage ◽  
Cooked Ham ◽  
End Products

The shelf life of vacuum-packed luncheon meats during refrigerated storage was not related to "total" counts since the only organisms that multiplied in this environment were lactic acid bacteria, which formed only a small proportion of the initial population.Bacterial growth curves obtained from several Canadian products were remarkably similar, but the spoilage patterns differed. For example, wiener spoilage was a function of bacterial growth while bologna spoiled from the accumulation of bacterial end products. After 15 weeks at 5 °C, the meats contained 0.6 to 0.8% lactic acid and the pH of comminuted meats dropped below 5.0. Cooked ham contained much less carbohydrate than comminuted meats, and the pH remained above 6.0. Under these conditions, ham is susceptible to putrefaction although this was not observed during this study.It was found that cooking can eliminate all lactic acid bacteria present in the raw meats but the products become recontaminated during slicing and packaging. The cleaning and sanitizing procedure used by the Company that cooperated in this study was efficient, but it was recommended that the packaging room be cleaned more often, and that a selective medium for lactic acid bacteria be used in sanitation surveys.

scholarly journals PENAMBAHAN BAKTERI ASAM LAKTAT UNTUK MENINGKATKAN KUALITAS, KEAMANAN DAN DAYA SIMPAN ROTI

Pro Food ◽  
2018 ◽  
Vol 4 (2) ◽  
pp. 333
Author(s):  
Moegiratul Amaro ◽  
Mutia Devi Ariyana ◽  
Wiharyani Werdiningsih ◽  
Baiq Rien Handayani ◽  
Nazaruddin Nazaruddin ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Shelf Life ◽  
Starter Culture ◽  
Food Additives ◽  
Microbiological Quality ◽  
Fermented Food ◽  
Natural Preservative ◽  
E Coli ◽  
Baking Process

ABSTRACT The changes of people consumtion patterns demands a healthier bread product and tends to be organic food without use synthetic food additives that brings various consequences to health. This condition leads to the development of food additives which has effectiveness equivalent to synthetic food additive but safe for health especially if consumed long term. Lactic acid bacteria is a bacteria that hass been widely used in fermented food production process. Making bread using lactic acid bacteria begins with sourdough or acid dough consisting of flour and water fermented with lactic acid bacteria either derived from a particular natural contaminant from flour or from a starter culture containing one or more known lactic acid bacteria. Various product of actic acid bacteria metabolism such as lactic acid, acetic acid, exopollysaccharide and antimicrobial compounds such as bacteriocin make lactic acid bacteria application in baking process have to the potential to improve microbiological quality and bread shelf-life. This research aims to study the effect of lactic acid bacteria addition as a natural preservative in the baking process. The ability of lactic acid bacteria to evolve naturally from fermented flour and water promises the potential for easy sourdough preparation and can be used continuously as  a natural preservative that will save production cost. The parameters to be determined include evaluation of lactic acid bacteria and yeast growth on dough and determination of moisture content as well as total bacteris, mold and E.coli an bread. Key words: bread, lactic acid bacteria, preservatives, shelf-life, food safety ABSTRAK Pergeseran pola konsumsi masyarakat menuntut adanya produk roti yang lebih sehat dan cenderung bersifat organik tanpa penggunaan Bahan Tambahan Makanan (BTM) sintetis yang membawa berbagai konsekuensi terhadap kesehatan. Kondisi ini mengarah pada berkembangnya pencarian BTM yang memiliki efektifitas yang setara dengan BTM sintetis namun aman bagi kesehatan terutama jika dikonsumsi jangka panjang. Bakteri Asam Laktat (BAL) merupakan golongan bakteri yang telah digunakan secara luas dalam proses produksi makanan fermentasi. Pembuatan roti menggunakan BAL diawali dengan sourdough atau adonan asam yang terdiri atas tepung dan air yang difermentasi dengan BAL baik yang berasal dari  kontaminan alami tertentu dari tepung atau dari suatu kultur starter yang mengandung satu atau lebih BAL yang sudah diketahui jenisnya. Berbagai produk hasil metabolisme BAL seperti asam laktat, asam asetat, eksopolisakarida dan senyawa antimikroba seperti bakteriosin menjadikan aplikasi BAL pada proses pembuatan roti berpotensi meningkatkan kualitas mikrobiologis dan daya simpan roti. Penelitian ini secara khusus bertujuan untuk mempelajari pengaruh penambahan BAL sebagai pengawet alami dalam proses pembuatan roti. Kemampuan BAL untuk berkembang secara alami dari tepung dan air yang difermentasi menjanjikan potensi penyediaan sourdough yang mudah dibuat dan dapat digunakan secara kontinyu sebagai pengawet alami sehingga akan menghemat biaya produksi. Parameter yang akan ditentukan meliputi evaluasi pertumbuhan BAL dan yeast pada adonan dan penentuan kadar air serta total bakteri, kapang dan E. coli pada roti.   Kata kunci: roti, bakteri asam laktat, pengawet, daya simpan, keamanan pangan.

scholarly journals Effect of antimicrobial agents on some chemical and microbiological characteristics of vacuum: Packaged ground buffalo meat stored under refregerated condition

2009 ◽  
Vol 25 (3-4) ◽  
pp. 231-239 ◽  
Author(s):  
M.A. Kenawi ◽  
Abdel Salam ◽  
M.N. Kenawi
Keyword(s):  
Sodium Chloride ◽  
Shelf Life ◽  
Antimicrobial Agents ◽  
Storage Period ◽  
Bacterial Count ◽  
Sodium Lactate ◽  
Plate Count ◽  
Buffalo Meat ◽  
Microbiological Characteristics ◽  
Aerobic Plate Count

The chemical characteristics and microbiological examination of vacuum packaged ground buffalo meat during cold storage for 24 days were determined in order to evaluate two different antimicrobial agents sodium lactate, sodium chloride and their mixture. The results indicated that the use of sodium lactate with or without sodium chloride delayed the proliferation of aerobic plate count, psychotropic count, and lactic acid bacterial count, and extended the shelf life up to more than 24 and 21 days respectively, compared to 8 days for the control samples. The TBA values for the samples treated by sodium chloride was not affected, while at the end of storage period the TBA values of samples treated by sodium lactate were significantly reduce the oxidation changes. Therefore, the use of sodium lactate with or without sodium chloride could be used to extend the shelf life of ground buffalo meat during refrigerated storage by reducing both chemical and microbiological changes. .

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