scholarly journals Eco-friendly Management of Blast Disease in Aromatic Rice by Formulated Antagonistic Fluorescent Pseudomonads under Natural Epiphytotic Condition

2021 ◽  
pp. 1
Author(s):  
Shila Chakraborty ◽  
Md Islam ◽  
Sabmira Silvi ◽  
Md Khokon
Keyword(s):  
Fluorescent Pseudomonads ◽  
Blast Disease ◽  
Aromatic Rice

scholarly journals Aromatic Rice with Multiple Disease Resistance Developed through Anther Culture

2021 ◽  
Vol 12 (5) ◽  
pp. 431-440
Author(s):  
Pawankumar Kharate ◽  
◽  
Pratik Pusadkar ◽  
Nirmala Bharti Patel ◽  
Shrinkhla Maurya ◽  
...  
Keyword(s):  
Anther Culture ◽  
Blast Resistance ◽  
Rice Variety ◽  
Blast Disease ◽  
Aromatic Rice ◽  
Disease Score ◽  
Short Period ◽  
Dh Lines ◽  
Moderate Resistance ◽  
Moderately Resistant

The experiments were conducted during kharif (June to September) 2018 and 2019 to screen 7 aromatic rice double haploid (DH) lines developed against Bacterial Leaf Bligh (BLB) and blast disease. Three genes Xa21, xa13, xa5 for BLB resistance and two genes Pi1, Pi2 for blast resistance was introgressed into a local aromatic rice variety Dubraj from Chhattisgarh State of India. Dubraj is extremely popular aromatic short medium grain variety in Chhattisgarh and Madhya Pradesh region and hence used in this study. RPBIO-226 and PR122 rice variety is used as donor for BLB and blast resistance respectively. 7 DH lines (L-1 to L-7) were developed from the crosses (Dubraj X RPBIO-226 X PR122) in the year 2017. Phenotypic evaluation for BLB diseases resistance has shown that L-1, L-6 and L-7 (score 1) has a similar resistance as the control check RPBIO-226(score-1) and 3 lines with moderate resistance (L-3 score 2) (L-4 score 3) (L-5 score 3). For blast disease, three lines (L-4, L-6 and L-7) were identified as resistance with the disease score of 1, whereas three were grouped under the section moderately resistant (L-1, L-3 and L- 5) with a disease score of 3. Sensory test (1.7% KOH) was conducted for the estimation of presence / absence of aroma. Moderately strong aroma was present in four lines (L-1, L-3, L-6 and L-7). On the basis of yield and presence of aroma in grain, L-3 has been selected with moderately resistant to BLB and blast for further advancement and subsequently evaluated under State Initial Evaluation Trial (SIET) during kharif 2020. Within 3 years of development of DH line, L-3 is under varietal developmental trail, which itself indicate the efficacy of anther culture in fixing homozygosity and speedy development of desired variety within short period of time.

scholarly journals Genotyping for Blast (Pyricularia oryzae) Resistance Genes in F2 Population of Supa Aromatic Rice (Oryza sativa L.)

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
L. Kanyange ◽  
J. Kamau ◽  
O. Ombori ◽  
A. Ndayiragije ◽  
M. Muthini
Keyword(s):  
Host Plants ◽  
Oryza Sativa L ◽  
Blast Resistance ◽  
Rice Variety ◽  
Snp Markers ◽  
Pyricularia Oryzae ◽  
Blast Disease ◽  
Aromatic Rice ◽  
R Genes ◽  
F2 Population

The ascomycete fungus, Pyricularia oryzae or Magnaporthe oryzae, is known to cause blast disease in more than 80 host plants of the Gramineae family—cereals including rice and grasses. The improvement of the Supa234 rice line (IR97012-27-3-1-1-B, containing badh2 gene for aroma) developed at IRRI-ESA Burundi consisted of introgression of R genes (Pita and Pi9) for blast resistance. The F2 population obtained via the cross had been screened for blast resistance using inoculation with Pyricularia oryzae spore’s suspension. The objectives of this study were to assess the presence of Pita and Pi9 genes for blast resistance and to assess the presence of the badh2 gene for aroma in the screened F2 plants using molecular markers. Genotyping was carried out in 103 F2 plants which grew to maturity using the KASP genotyping method with SNP markers (snpOS0007, snpOS0006, and snpOS0022) targeting the Pita and Pi9 genes for blast resistance and the badh2 gene for aromatic fragrance. The genotyping results showed that 38 F2 plants had the Pita gene present in both alleles, 31 F2 plants with the Pita gene in one allele, and only one plant (3B1) was found with the Pi9 gene in one allele. The badh2 gene for aroma was detected in 27 F2 plants on both alleles and in 57 F2 plants on one allele. There were thirteen plants which had both the Pita gene and the badh2 gene for aroma, and only one plant (3B1) had a combination of the three genes (Pita, Pi9, and badh2). Seven plants resistant to blast disease (2H2, 2H4, 1G2, 1C12, 1E13, 1B12, and 1C5) with the Pita and badh2 genes were found, and only one resistant plant (3B1) had a combination of the three genes Pi9, Pita, and badh2 which is recommended to be bulked for the development of the Supa aromatic rice variety resistant to blast disease. The plants generated by the best line 3B1 should further be evaluated for grain quality (Supa type) after F5 generation in the field.

Neck blast disease influences grain yield and quality traits of aromatic rice

2014 ◽  
Vol 337 (11) ◽  
pp. 635-641 ◽  
Author(s):  
Mohammad Ashik Iqbal Khan ◽  
Md Rejwan Bhuiyan ◽  
Md Shahadat Hossain ◽  
Partha Pratim Sen ◽  
Anjuman Ara ◽  
...  
Keyword(s):  
Grain Yield ◽  
Blast Disease ◽  
Aromatic Rice ◽  
Quality Traits ◽  
Yield And Quality ◽  
Neck Blast ◽  
Grain Yield And Quality

scholarly journals Evaluation of indigenous aromatic rice cultivars from sub-Himalayan Terai region of India for nutritional attributes and blast resistance

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Debayan Mondal ◽  
Prudveesh Kantamraju ◽  
Susmita Jha ◽  
Gadge Sushant Sundarrao ◽  
Arpan Bhowmik ◽  
...  
Keyword(s):  
Nutritive Value ◽  
Amylose Content ◽  
Blast Resistance ◽  
Starch Content ◽  
Low Cost ◽  
Principal Component ◽  
Blast Disease ◽  
Basmati Rice ◽  
Phenotypic Traits ◽  
Rice Cultivars

AbstractIndigenous folk rice cultivars often possess remarkable but unrevealed potential in terms of nutritional attributes and biotic stress tolerance. The unique cooking qualities and blissful aroma of many of these landraces make it an attractive low-cost alternative to high priced Basmati rice. Sub-Himalayan Terai region is bestowed with great agrobiodiversity in traditional heirloom rice cultivars. In the present study, ninety-nine folk rice cultivars from these regions were collected, purified and characterized for morphological and yield traits. Based on traditional importance and presence of aroma, thirty-five genotypes were selected and analyzed for genetic diversity using micro-satellite marker system. The genotypes were found to be genetically distinct and of high nutritive value. The resistant starch content, amylose content, glycemic index and antioxidant potential of these genotypes represented wide variability and ‘Kataribhog’, ‘Sadanunia’, ‘Chakhao’ etc. were identified as promising genotypes in terms of different nutritional attributes. These cultivars were screened further for resistance against blast disease in field trials and cultivars like ‘Sadanunia’, ‘T4M-3-5’, ‘Chakhao Sampark’ were found to be highly resistant to the blast disease whereas ‘Kalonunia’, ‘Gobindabhog’, ‘Konkanijoha’ were found to be highly susceptible. Principal Component analysis divided the genotypes in distinct groups for nutritional potential and blast tolerance. The resistant and susceptible genotypes were screened for the presence of the blast resistant pi genes and association analysis was performed with disease tolerance. Finally, a logistic model based on phenotypic traits for prediction of the blast susceptibility of the genotypes is proposed with more than 80% accuracy.

scholarly journals Farmers’ knowledge and perception of finger millet blast disease and its control practices in western Kenya

2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Wilton Mbinda ◽  
Agnes Kavoo ◽  
Fredah Maina ◽  
Margaret Odeph ◽  
Cecilia Mweu ◽  
...  
Keyword(s):  
Finger Millet ◽  
Management Practices ◽  
Smallholder Farmers ◽  
Descriptive Analysis ◽  
Wide Distribution ◽  
Blast Disease ◽  
Eastern Africa ◽  
Planting Density ◽  
Global Food Security ◽  
Disease Occurrence

Abstract Background Finger millet blast disease, caused by Pyricularia oryzae, is a serious constrain of finger millet production which is threatening global food security especially to the resource poor smallholder farmers in arid and semi-arid regions. The disease adversely affects finger millet production and consumption due to its wide distribution and destruction in all finger millet growing areas of southern Asia and eastern Africa. Here, we present a study that investigated the occurrence, impact, risk factors and farmers’ knowledge and perceptions of finger millet blast in Kenya. Methods We surveyed blast disease occurrence and interviewed farmers in Bungoma and Kisii Counties of Kenya during March–April 2019. Data were analysed using SPSS statistical program. Descriptive analysis was done by calculating means, percentages, frequencies, and standard errors. Comparative statistics, chi-square and t-tests, were used to evaluate differences existing among the farm characteristics and socio-demographics and the knowledge and perceptions of blast disease and its management practices. Results Our results show that blast disease is prevalent in all surveyed areas and adversely affects the productivity of the crop leading to poor yields. The disease occurrence varied from 92 to 98%, and was significantly higher in the major finger millet growing areas compared to the minor ones. Blast occurrence was associated with rainfall, altitude, planting density, intercropping and other farming practices. In all the surveyed regions, farmers had little knowledge about blast disease identification, its detection and spread. Further, the farmers’ awareness of blast disease control was inconsistent with established practices. Conclusions Our results show mitigation of finger millet blast disease should aim at improving farmers’ adoption of best practices through development of acceptable blast-resistant finger millet varieties, use of sustainable disease management practices, fostering linkages and creating new partnerships in the production-supply chain and maintaining a functional seed system. Findings from this study provide essential insights for effective decision making and management of the disease. This is fundamental to sustainable and secure food and income for finger millet growing farmers in Kenya.

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