In vitro-scientific evaluation on anti-Candida albicans activity, antioxidant properties and phytochemical constituents with the identification of antifungal active fraction from traditional medicinal plant Couroupita guianensis Aubl. Flower

Objective: To estimate the in vitro total phenolics, flavonoids contents, antioxidant and antimicrobial activities of various solvent extracts from the medicinal plant Physalis minima Linn.Methods: The crude bioactive were extracted from the dried powder of Physalis minima using methanol, ethyl acetate, chloroform and hexane solvents. Total phenolic content (TPC) and total flavonoid content (TFC) were estimated using Folin-Ciocalteu and aluminium chloride colorimetric methods respectively. 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) assays were used to determine the in vitro antioxidant capacity. The antimicrobial assay was done through agar well diffusion; minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using broth microdilution methods against the Gram-negative bacteria (Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris) and Gram-positive bacteria (Staphylococcus aureus).Results: TPC expressed as gallic acid equivalents (GAE) ranged from 60.27±1.73-151.25±2.50 mg GAE/g dry weight, and TFC expressed as quercetin equivalents (QE) ranged from 56.66±0.80-158.84±2.30 mg QE/g dry weight. Methanol extract showed the highest antioxidant activity followed by ethyl acetate, chloroform, hexane extract and the IC50 values of methanol extract for scavenging DPPH and ABTS free radicals were 280.23±5.75-173.40±0.38µg/ml, respectively. All the extracts have shown potent antimicrobial activity for the zone of inhibition ranged from 9-35 mm; MICs and MBCs values ranged from 0.125-4.0 and 0.25-8.0 mg/ml, respectively towards tested pathogenic species.Conclusion: The comprehensive analysis of the present results demonstrated that Physalis minima possess high potential antioxidant properties which could be used as a viable source of natural antioxidants in treating infections caused by above-mentioned pathogens.

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Antifungal Activity of the Essential Oil of Echinops kebericho Mesfin: An In Vitro Study

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/3101324 ◽

2020 ◽

Vol 2020 ◽

pp. 1-7

Author(s):

Tamirat Bekele Beressa ◽

Serawit Deyno ◽

Paul E. Alele

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Essential Oil ◽

Cryptococcus Neoformans ◽

Antioxidant Properties ◽

Pathogenic Fungi ◽

Diffusion Method ◽

Potential Candidate ◽

Standard Drug

Background. Echinops kebericho is an endemic medicinal plant in Ethiopia widely used in the treatment of infectious and noninfectious diseases. Essential oils are known for their antibacterial, antifungal, antiviral, insecticidal, and antioxidant properties. This study evaluated the antifungal activity of essential oil from E. kebericho against four common pathogenic fungi and two standard strains. Methods. The essential oil was obtained by hydrodistillation. The antifungal screening was done by agar well diffusion method. Minimal inhibitory concentrations (MICs) were determined by broth microdilution. Minimal fungicidal concentrations (MFCs) were determined by subculturing fungal strains with no visible growth onto a Sabouraud dextrose agar (SDA) plate. Results. Candida albicans and Cryptococcus neoformans were highly sensitive while Aspergillus flavus did not show sensitivity up to 1 mg/ml of essential oil; MICs ranged from 0.083 mg/ml to 0.208 mg/ml. Concentration and fungal species showed significant dose-dependent associations ( p < 0.0001 ) with antifungal activity. The MICs of essential oil were comparable to those of the standard drug (fluconazole) against C. glabrata and C. krusei. The lowest MFC of the essential oil was observed against Candida parapsilosis (0.145 mg/ml) while the highest MFC was against Candida krusei (0.667 mg/ml). Conclusion. Echinops kebericho essential oil showed noteworthy antifungal activity against Cryptococcus neoformans, Candida albicans, and Candida glabrata and could be a potential candidate for further antifungal drug development.

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In vitro—Scientific evaluation on antimicrobial, antioxidant, cytotoxic properties and phytochemical constituents of traditional coastal medicinal plants

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2016.07.019 ◽

2016 ◽

Vol 83 ◽

pp. 648-657 ◽

Cited By ~ 13

Author(s):

Santhiyagu Prakash ◽

Ramasamy Ramasubburayan ◽

Vijayan Sri Ramkumar ◽

Ethiraj Kannapiran ◽

Arunachalam Palavesam ◽

...

Keyword(s):

Medicinal Plants ◽

Scientific Evaluation ◽

Phytochemical Constituents

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PHYTOCHEMICAL EVALUATION AND IN VITRO ANTIOXIDANT STATUS OF CLERODENDRUM VOLUBILE (AN INDIGENOUS MEDICINAL PLANT)

Pakistan Journal of Pharmaceutical Research ◽

10.22200/pjpr.2016277-88 ◽

2016 ◽

Vol 2 (2) ◽

pp. 77 ◽

Cited By ~ 8

Author(s):

Tomisin Happy Ogunwa ◽

Tolulope Tosin Adeyelu ◽

Rotimi Yemi Fasimoye ◽

Mary Bose Oyewale ◽

Taiwo Ademola Ademoye ◽

...

Keyword(s):

Ascorbic Acid ◽

Medicinal Plant ◽

Research Work ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Dry Weight ◽

Natural Antioxidants ◽

Therapeutic Effects ◽

Dpph Radical

ABSTRACTClerodenrum volubile is an important locally used medicinal plant. It is one of the essential herbs nature has provided for mankind to be consumed as spices, vegetable and also used in the treatment of diseases. Qualitative phytoconstituent screening of the plant revealed the presence of steroids, flavonoids, tannins, saponins, and phenolic compound while chalcones, alkaloid and anthraquinone were absent. Quantitative phytochemical evaluation showed values of 8.29±1.26mg/g rutin equivalent for flavonoids, 3.53±0.05mg/g gallic acid equivalent for phenol, 3.97±0.03mg/g tannic acid equivalent for tannins and 13.67±1.27% for saponin per dry weight. As it is imperative to extend research work on therapeutic effects of the arsenal of plants nature has given to us in Africa so as to obtain a cure for various diseases attacking human’s health, antioxidant properties of the plant was evaluated. Antioxidant models used include iron chelating, DPPH radical, superoxide ion, hydrogen peroxide, ABTS radical, hydroxyl radical scavenging activities and ferric ion reducing properties. The obtained IC50 values against DPPH radical were 141.342 and 120.349µg/mL for Clerodendrum volubile and trolox respectively. The chelating effect of the plant extract at 50% inhibition was close to that of ascorbic acid (standard) with 134.34 and 131.19µg/mL concentration respectively. Overall, the aqueous extract of the plant showed antioxidant potential which was close to the effects exerted by known standard antioxidants (ascorbic acid, trolox and EDTA). The plant could hence provide natural antioxidants which are needed to combat numerous free radical-mediated diseases and complications such as aging, cancer, atherosclerosis, which are linked with oxidative stress.

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Phytochemicals of Apple Pomace as Prospect Bio-Fungicide Agents against Mycotoxigenic Fungal Species—In Vitro Experiments

Toxins ◽

10.3390/toxins11060361 ◽

2019 ◽

Vol 11 (6) ◽

pp. 361 ◽

Cited By ~ 4

Author(s):

Marta Oleszek ◽

Łukasz Pecio ◽

Solomiia Kozachok ◽

Żaneta Lachowska-Filipiuk ◽

Karolina Oszust ◽

...

Keyword(s):

Antifungal Activity ◽

Antioxidant Properties ◽

Fungal Species ◽

Apple Pomace ◽

Quercetin Derivatives ◽

Spectroscopic Analyses ◽

Neosartorya Fischeri ◽

Quercetin Glycosides ◽

Phytochemical Constituents

The phytochemical constituents of apple waste were established as potential antifungal agents against four crops pathogens, specifically, Botrytis sp., Fusarium oxysporum, Petriella setifera, and Neosartorya fischeri. Crude, purified extracts and fractions of apple pomace were tested in vitro to evaluate their antifungal and antioxidant properties. The phytochemical constituents of the tested materials were mainly represented by phloridzin and quercetin derivatives, as well as previously undescribed in apples, monoterpene–pinnatifidanoside D. Its structure was confirmed by 1D- and 2D-nuclear magnetic resonance (NMR) spectroscopic analyses. The fraction containing quercetin pentosides possessed the highest antioxidant activity, while the strongest antifungal activity was exerted by a fraction containing phloridzin. Sugar moieties differentiated the antifungal activity of quercetin glycosides. Quercetin hexosides possessed stronger antifungal activity than quercetin pentosides.

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Phytochemistry and Pharmacological Studies ofCitrus macroptera: A Medicinal Plant Review

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2017/9789802 ◽

2017 ◽

Vol 2017 ◽

pp. 1-7 ◽

Cited By ~ 5

Author(s):

Koly Aktar ◽

Tahira Foyzun

Keyword(s):

Medicinal Plant ◽

Antioxidant Enzyme ◽

Folk Medicine ◽

Present Review ◽

Medicinal Uses ◽

Pharmacological Studies ◽

Phytochemical Constituents ◽

Pharmacological Potential ◽

Comprehensive Study

Citrus macroptera(family Rutaceae), commonly known as Sat Kara, is a pharmacologically diverse medicinal plant. Various parts of this plant, specifically fruit, have an immense range of medicinal uses in folk medicine directed for a number of ailments. A plethora of active phytochemical constituents of this plant have been revealed so far, namely, limonene, beta-caryophyllene, beta-pinene, geranial edulinine, ribalinine, isoplatydesmine, and so forth. Several studies demonstrated the exploration of pharmacological potential of various parts such as fruits, leaves, and stems ofC. macropteraas antioxidant, cytotoxic, antimicrobial, thrombolytic, hypoglycemic, anxiolytic, antidepressant, cardioprotective, and hepatoprotective. Furthermore, inhibition of in vitroα-amylase, inhibition of paracetamol induced hepatotoxicity, and potentiation of brain antioxidant enzyme are also ascertained. In present review, comprehensive study focused on knowledge regarding several phytopharmacological activities ofCitrus macropterahas been described.

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Epilobium angustifolium L.: A medicinal plant with therapeutic properties

The EuroBiotech Journal ◽

10.24190/issn2564-615x/2017/02.03 ◽

2017 ◽

Vol 1 (2) ◽

pp. 126-131

Author(s):

Halyna Ostrovska ◽

Oleksandra Oleshchuk ◽

Samuele Vannini ◽

Samuela Cataldi ◽

Elisabetta Albi ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Medicinal Plant ◽

Antioxidant Properties ◽

Carcinoma Cells ◽

High Concentration ◽

Mitochondrial Potential ◽

Low Concentration ◽

Epilobium Angustifolium ◽

Human Hepatocellular Carcinoma Cells

Abstract Epilobium angustifolium L. is a medicinal plant belonging to the Onagraceae family, which includes more than 200 different species from all over the world. Traditional medicinal applications include treatment of prostate, gastrointestinal, menstrual disorders and recently it has been used for its analgesic and anti-inflammatory activity. In this investigation E. angustifolium was collected in Ternopil region of Ukraine. The obtained data demonstrated that E. angustifolium herb extract, rich in polyphenolic compounds such as flavonoids and tannins, display high antioxidant properties. In addition the potential anticancer activity has been investigated in vitro on human hepatocellular carcinoma cells (HepG2). Furthermore the cytotoxic and genotoxic effects of E. angustifolium have been investigated respectively by MTT and Comet assay. Results showed that at low concentration, up to 25 μg/mL, the cytotoxic effect was not observed. Increasing concentration from 50 to 75 μg/mL reduced significantly cell viability and induced an important DNA damage in hepatocellular carcinoma. These promising data were also confirmed with mitochondrial potential test. It is possible to conclude that E. angustifolium has beneficial properties in low concentration, in term of antioxidant activity, and it could be a potential antitumoral natural product if it will be used at high concentration

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