scholarly journals Effect of treatment time on performance of nano-encapsulatedfluoride de ntifrices for remineralization of initial carious lesions: an in vitro study

2021 ◽  
Vol 34 (1) ◽  
pp. 56-62
Author(s):  
Andressa Oliveira ◽  
Elizabeth Sousa ◽  
Nayanna Fernandes ◽  
Ingrid Meira ◽  
Juliane Lavôr ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Treatment Time ◽  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Vitro Study ◽  
Carious Lesion ◽  
Time Data ◽  
Carious Lesions

The aim of this in vitro study was to evaluate the influence of treatment time on the remineralization performance of nanoencapsulated fluoride dentifrices on initial carious lesions. Ninety-six human enamel samples were allocated to eight groups (n = 12): 50% NanoF + 50% free NaF, 100% NanoF, 100% NaF (positive control), and placebo (negative control), using two different treatment times (one and five minutes) for each dentifrice tested. After the carious lesion induction, the specimens were submitted to a pH remineralizing cycling model for seven days. Surface microhardness was measured before and after carious lesion induction and after treatment. The percentage of surface remineralization was calculated for each study time. Data were analyzed using two-way ANOVA and ANOVA repeated-measures tests followed by the Bonferroni correction (p < 0.05). Remineralization differences were observed in the dentifrices analyzed according to the treatment time used. NanoF formulations with 50% (one-min treatment) and 100% (five-min treatment) promoted significant remineralization of enamel after the caries challenge when compared to the placebo dentifrice (p < 0.05). Thus, time was considered an important factor for the fluoride release system. Nanotechnology can be a promising system for caries remineralization as it makes fluoride available on the dental surface for a longer time.

Anti-allergic assessment of ethanol extractives of Quisqualis Indica Linn.

2020 ◽  
Vol 16 ◽  
Author(s):  
Deepa Chaudhary ◽  
Rajnish Srivastava ◽  
Hemant Nagar
Keyword(s):  
Mast Cells ◽  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Vitro Study ◽  
Control Group ◽  
Disodium Cromoglycate ◽  
Ethanol Extracts

Aim:: The present work was aimed to find out the anti-allergic activity of ethanol extracts of Quisqualis indica Linn. (EEQI) by in-vitro and in-vivo murine models. Background:: Worldwide, the rise in prevalence of allergic diseases has continued in the industrialized world for more than 50 years. Worldwide, 0.05–2% of the population is estimated to experience anaphylaxis at some point in life. Quisqualis Indica Linn in an ornamental plant that have been rarely used as a herbal medicines, however presence of polyphenols and flavonoids have been reported to possessed anti-inflammatory, antipyretic and immunomodulatory activity which have some pathological relevance with anaphylaxis. Objective:: The objective of the present research was to investigate, scientifically explored and understand the probable antianaphylactic mechanism of ethanol extracts of Quisqualis indica Linn. via different preclinical models. Material and Method:: In-vitro study was done on de-granulated mesenteric mast cells induced by compound 48/80 and invivo study was done by passive cutaneous anaphylaxis (PCA) model. In the in-vitro study degranulated mesenteric cells were grouped into negative control (compound 48/80 treated), positive control (Disodium cromoglycate + 48/80 treated) and 3 test groups (EEQI 10 μg/ml + 48/80 treated, EEQI 50 μg/ml + 48/80 treated and EEQI 100 μg/ml + 48/80 treated). The number of degranulated mast cells was counted and compared within the different treatment groups. In the in-vivo study the rats were first grouped into negative control (vehicle only), positive control (Disodium cromoglycate) and 2 test groups (EEQI: 100 and 200 mg/kilogram). The animals were pretreated for 12 days. On the 12th day all the rats were immunized with serum anti-ovalbumin (obtained from an already sensitized rat) by the intradermal route. After 24 h of serum injection, Evans blue dye containing oval albumin was administered intravenously in all groups. Three days later, the rats were taken down for the severity of the anaphylactic reactions. Result:: EEQI significantly attenuate mast cell degranulation and maintain the cell intactness as compared to control (P < 0.001). It was set up to support the degree of anaphylaxis as compared to control group (P < 0.001). Conclusion:: The outcomes of the work revealed the preventive effect of Quisqualis indica Linn. against allergic manifestations.

RESULTS OF INVESTIGATION OF THE EFFECTS OF BOVINE PLACENTAL PREPARATION ON IMMUNE FUNCTION

2018 ◽  
Vol 21 (02) ◽  
pp. 22-28
Author(s):  
Dolgorsuren Ts ◽  
Lkhagvasuren N ◽  
Batsaikhan D ◽  
Erdenechimeg D ◽  
Oyunnomin N ◽  
...  
Keyword(s):  
Cell Division ◽  
Stimulation Index ◽  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Vitro Study ◽  
Spleen Weight ◽  
Spleen Index

The present study aimed to investigate the effects of bovine placental preparation under in vitro and in vivo conditions. Cell Proliferation Kit I (MTT) was used for in vitro study of placental preparation effect to proliferate lymphocytes. Lymphocytes were isolated from spleen of Balb C mice, 100 μl cell was added to each well of 96 well culture plate, followed by addition of 10 μl placental preparation and mitogen (concanavalin-A) separately and in combination and cell culture only as control was used. Results were obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with ELISA microplate reader. Effect of placental preparation to proliferate lymphocytes in vivo condition was investigated in mice, which were divided into 4 groups and 4 subgroups. The results were estimtaed with spleen weight, spleen index and splenocyte counts. Results of in vitro study demonstrated that stimulation index increased by 1.19 or 19% for cell division in wells to which no mitogen was added, but the preparation was added as compared to control wells, cell division index increased with 1.38 or 38% for cell division in wells to which mitogen was added as compared to control wells and stimulation index was higher by 1.68 or 68% for cell division in wells with cells to which both mitogen and preparation were added than control wells. For in vivo experiments, spleen index and splenocyte count for animals of positive control subgroup-1 treated once by 0.2 ml sheep red blood cells were greater by 1.38 and 1.5 times respectively than relevant negative control animals, whereas spleen index and splenocyte count for animals of experimental subgroup -1 were greater by 3.09 and 2.2 times respectively. For animals of positive control subgroup -2, both spleen index and splenocyte count decreased by 1.35 and 1.3 times respectively than negative control animals, whereas they dropped by 1.1 and 1.17 times respectively in mice of experimental subgroup -2. Spleen index and splenocyte count in mice treated with the preprartion only increased by 1.2 times or 20% as compared to negative control animals. From above results, it is shown that bovine placental preparation is able to exert immunomodulatory effect regardless of antigen under both in vitro and in vivo conditions.

scholarly journals Evaluation of Different Dentifrice Compositions for Increasing the Hardness of Demineralized Enamel: An in Vitro Study

2019 ◽  
Vol 7 (1) ◽  
pp. 14
Author(s):  
Pedro Henrique Cabral Oliveira ◽  
Marcia Regina Cabral Oliveira ◽  
Luiz Henrique Cabral Oliveira ◽  
Ravana Angelini Sfalcin ◽  
Marcelo Mendes Pinto ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Carious Lesions ◽  
Demineralized Enamel ◽  
Ph Cycling ◽  
Bovine Enamel ◽  
Knoop Indenter

This study aimed to evaluate microhardness of a dentifrice containing fluoride and arginine compared to a positive control (fluoride only) and a negative control (no fluoride) on sound and demineralized bovine enamel surfaces. Specimens were randomly assigned to different treatments that included daily pH cycling and brushing three times a day with one of the following dentifrices (n = 8): Neutraçucar (arginine and fluoride), Colgate Total 12 (fluoride) and My First Colgate (no fluoride). Enamel carious lesions were artificially created one week before the beginning of these treatments (demineralized bovine enamel (DE) groups). The same groups were also tested in sound enamel (sound bovine enamel (SE) groups). Microhardness was measured at baseline and after one, two, and five weeks of treatment using a Knoop indenter. Statistical analysis involved two-way Analysis of Variance (ANOVA) and Tukey’s test. After five weeks, both Total 12 and Neutraçucar had increased the microhardness of DE specimens (p < 0.05). Only Neutraçucar had increased the microhardness of the sound enamel after five weeks of treatment. Thus, it could be concluded that arginine-based dentifrices increase the microhardness of sound and demineralized bovine enamel surfaces.

scholarly journals Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study

2016 ◽  
Vol 45 (4) ◽  
pp. 234-239 ◽  
Author(s):  
Priscilla Barbosa Ferreira SOARES ◽  
Aletheia Moraes ROCHA ◽  
Manuella Verdinelli de Paula REIS ◽  
Camilla Christian Gomes MOURA ◽  
Carlos José SOARES
Keyword(s):  
Cell Viability ◽  
Tap Water ◽  
Human Fibroblasts ◽  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Coconut Water ◽  
Ph Adjustment ◽  
Adjustment Method

Abstract Objective This study evaluated four types of pH adjustment of the coconut water (CW) on viability of human fibroblasts (HFF). Material and method Natural and industrialized CW were adjusted to pH 7.0 using: (1) Sodium Hidroxide (NaOH), (2) Sodium bicarbonate (NaHCO3), (3) Triethanolamine (C6H15NO3), (4) 2-Amino-2-Methil-1-Propanol (C4H11NO). Fibroblasts were plated at 2×104/ well in 96 well plates and maintained in the CW solutions for 2 h and 4 h. Positive control was represented by HFF maintained in DMEM and the negative control by tap water. Cell viability was analyzed by MTT formazan method. Data were analyzed by 3-way ANOVA followed by Tukey’s and Dunnet’s test. Result There are no significant effect on the cell viability regarding type of CW, period of evaluation, and the interactions between CW and period of evaluation, CW and pH adjustment method, pH adjustment method and period of evaluation (p>0.05). Conclusion The product used for CW pH adjustment did not influenced HFF viability, thought there are a tendency of better performance in natural CW.

Evaluating Dentin Surface Treatments for Resin-Modified Glass Ionomer Restorative Materials

2013 ◽  
Vol 38 (4) ◽  
pp. 429-438 ◽  
Author(s):  
TA Imbery ◽  
A Namboodiri ◽  
A Duncan ◽  
R Amos ◽  
AM Best ◽  
...  
Keyword(s):  
Testing Machine ◽  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Surface Treatments ◽  
Band Matrices ◽  
Dentin Surface ◽  
Occlusal Surfaces ◽  
Significant Difference

SUMMARY This in vitro study evaluated the effect of six surface treatments on the shear bond strength of three resin-modified glass ionomers (RMGIs) to dentin. Occlusal surfaces of caries-free third molars were reduced to expose only dentin. Surface treatments were smear layer intact (negative control), Cavity Conditioner, EDTA, Ketac Primer, Self Conditioner, and etching with 35% phosphoric acid followed by the application of Optibond Solo Plus. Filtek Z250 composite resin bonded with Optibond Solo Plus served as a positive control. Conditioning agents were used according to the manufacturers' instructions. After surface treatments, Fuji II LC, Riva LC, Ketac Nano, and Filtek Z250 were placed in copper-band matrices 5 mm in diameter and 2 mm in height and were light-cured for 20 seconds. Specimens were stored in 100% humidity for 24 hours, after which they were placed in deionized water for 24 hours at 37°C. They were then tested under shear forces in an Instron Universal Testing Machine at a crosshead speed of 0.5 mm/min. A two-way analysis of variance and Tukey honestly significant difference statistical analyses (p&lt;0.05) indicated significant interaction between RMGIs and conditioning agents. Acid etching followed by Optibond Solo Plus provided highest bond strengths for all three RMGIs, which were not statistically different from the positive control.

Effect of Different Intermediary Bases on Microleakage of a Restorative Material in Class II Box Cavities of Primary Teeth

2017 ◽  
Vol 40 (2) ◽  
pp. 82-87 ◽  
Author(s):  
Faika Y. Abdelmegid ◽  
Fouad S. Salama ◽  
Waleed M. Al-Mutairi ◽  
Saud K. Al-Mutairi ◽  
Sultan O. Baghazal
Keyword(s):  
Primary Teeth ◽  
In Vitro Study ◽  
Class Ii ◽  
Positive Control ◽  
Negative Control ◽  
The Other ◽  
Control Groups ◽  
Significant Difference ◽  
The Mean

Introduction The aim of this in vitro study was to assess and compare the effect of different intermediary bases on microleakage between tooth and a nanocomposite interface in Class II box cavities in primary teeth. Methods Standard Class II box cavities were prepared in 52 primary molars and randomly divided into 9 groups according to the intermediary base used (Multicore Flow, Fuji II LC, SDR, Smart Dentin Replacement, and Biodentine). All specimens were subjected to thermocycling and prepared for microleakage testing and evaluation. Results There was significant difference in the mean ranks of microleakage between the 9 groups, which was observed in the gingival side (p<0.0001) and the occlusal side (p<0.0001). The mean ranks microleakage was significantly higher with experimental SDR, experimental Multicore Flow, and positive control materials when compared with the other 6 groups. The microleakage mean ranks were statistically significantly lower in experimental Fuji II LC, experimental Biodentine, and all negative control groups when compared with the other 3 groups. Conclusions Microleakage is affected by the application of intermediate material. Experimental Biodentine and Fuji II LC showed the lowest microleakage while experimental SDR and experimental Multicore Flow showed the highest microleakage.

In vitro study of the effect of a pulsed 10.6μm CO 2 laser and fluoride on the reduction of carious lesions progression in bovine root dentin

2010 ◽  
Author(s):  
Thaís M. Parisotto ◽  
Patrícia A. Sacramento ◽  
Marcelo C. Alves ◽  
Regina M. Puppin-Rontani ◽  
Maria Beatriz D. Gavião ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Carious Lesions ◽  
Root Dentin

scholarly journals In Vitro Evaluation of the Efficacy of Different Over-the-Counter Products on Tooth Whitening

2015 ◽  
Vol 26 (4) ◽  
pp. 373-377 ◽  
Author(s):  
Muhammet Karadas ◽  
Zeynep Yesil Duymus
Keyword(s):  
Repeated Measures ◽  
Positive Control ◽  
Negative Control ◽  
Carbamide Peroxide ◽  
Tooth Whitening ◽  
Over The Counter ◽  
Color Changes ◽  
Significant Difference ◽  
Cielab System

<p>This <italic>in vitro</italic> study evaluated the whitening effect of four different over-the-counter products compared with a 10% carbamide peroxide (CP) bleaching gel. Specimens obtained from bovine incisors were stained in a tea solution and randomly divided into six groups according to the product used (n=10): CT: conventional toothpaste (negative control); CWT: Crest 3D White toothpaste; CWR: Crest 3D White mouth rinse; CWS: Crest 3D White strips; DW: Dazzling White (paint-on gel); and OP: Opalescence PF 10% CP (at-home bleaching gel, positive control). Over-the-counter products and 10% CP were used according to the manufacturer's recommendations. Color measurements were performed according to the CIELab system using spectrophotometer after staining, and after 4 and 8 weeks of the treatment procedure. The ∆E, ∆L, ∆a, and ∆b values were calculated. The data obtained were analyzed by repeated-measures ANOVA. Tukey's test for multiple comparisons was applied (p<0.05). All groups, except CWT, were effective in tooth whitening in comparison with conventional toothpaste. There was no significant difference in tooth whitening between CWT and CT (p=0.93). CWS, DW and OP groups presented significantly higher color changes than the CWR group. DW and CWS showed similar tooth whitening to OP.</p>

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