scholarly journals Efrects Of Peritoneal Lavage And Dry Cleaning On Bacterial Translocation In Peritonite Model Created With Cecal Ligation Puncture

2018 ◽  
Author(s):  
Turgut Reis Koç
Keyword(s):  
Bacterial Translocation ◽  
Peritoneal Lavage ◽  
Cecal Ligation ◽  
Dry Cleaning ◽  
Cecal Ligation Puncture

scholarly journals TREATMENT OF ABDOMINAL SEPSIS WITH LEVAMISOLE AND PERITONEAL LAVAGE IN RATS

2020 ◽  
Vol 11 (2) ◽  
pp. 73-80
Author(s):  
Gabriela Vieira da Silva ◽  
Aldo Medeiros de Cunha ◽  
Ítalo Medeiros de Azevedo ◽  
Lívia Medeiros Soares Celani ◽  
Filipe Fernandes Santos de Oliveira
Keyword(s):  
Peritoneal Lavage ◽  
Tissue Injury ◽  
Cecal Ligation ◽  
Clinical Score ◽  
Abdominal Sepsis ◽  
Control Group ◽  
Treatment Needs ◽  
Lower Growth ◽  
Cecal Ligation Puncture ◽  
Difficult Situations

Introduction: Intra-abdominal sepsis is one of the most difficult situations in surgery and usually presents as a peritonitis. It is a systemic inflammatory response to infection that is often associated with hypoperfusion followed by tissue injury and organ failure. Given that its the sequelae reflect the increasing severity of the systemic response to infection, a correct and early treatment needs to be established. In this sense, the study analyzes the effect of peritoneal lavage and the use of the isomers immunomodulator in the treatment of abdominal sepsis in rats. Methods: The animals were randomly assigned to three groups A (control), B (peritoneal lavage), C (levamisole). All groups underwent laparotomy, anesthesia and cecal ligation/puncture. A ten-parameter clinical score was analyzed, which allowed the classification of sepsis degree, peritoneal fluid cultures, and cytokine dosage. Results: Group C had better scores on the clinical score and also lower growth of bacterial colonies in cultures. A lower dosage of TNF-alpha, IL-Ib, IL-6 and PCR in group C was observed, compared to groups A and B. Conclusion: Levamisole positively influenced the treatment in the parameters analyzed compared to the peritoneal lavage group and the control group.

EFFECTS OF TYRPHOSTIN AG-556 ON RAT SHOCK INDUCED BY CECAL LIGATION PUNCTURE OR LPS.

Shock ◽  
1998 ◽  
Vol 9 (Supplement) ◽  
pp. 33
Author(s):  
S. Ogura ◽  
O. Umegaki ◽  
I. B. Cochran ◽  
P. V. Halushka ◽  
J. A. Cook
Keyword(s):  
Cecal Ligation ◽  
Cecal Ligation Puncture

scholarly journals STAT4 Is Required for Antibacterial Defense but Enhances Mortality during Polymicrobial Sepsis

2001 ◽  
Vol 8 (6) ◽  
pp. 1044-1048 ◽  
Author(s):  
Christopher J. Godshall ◽  
Alex B. Lentsch ◽  
James C. Peyton ◽  
Melanie J. Scott ◽  
William G. Cheadle
Keyword(s):  
Peritoneal Lavage ◽  
Killer Cell ◽  
Neutrophil Migration ◽  
Cecal Ligation ◽  
Lavage Fluid ◽  
Interleukin 12 ◽  
Natural Killer Cell Cytotoxicity ◽  
Peritoneal Lavage Fluid ◽  
Bacterial Counts ◽  
Transcription Factor 4

ABSTRACT The signal transducer and activator of transcription factor 4 (STAT4) pathway mediates the intracellular effects of interleukin-12 (IL-12), leading to the production of gamma interferon, induction of a T helper type 1 response, and increased natural killer cell cytotoxicity. The purpose of this study was to determine the role of the STAT4 pathway during polymicrobial peritonitis in the cecal ligation and puncture (CLP) model. CLP was performed on STAT4-deficient (STAT4−/−) and wild-type control (BALB/c) mice. At 4 h after CLP, STAT4−/− mice had significantly higher bacterial counts in the peritoneal lavage fluid, liver, and blood. This difference persisted for 18 h in the peritoneal lavage fluid and blood. Neutrophil migration to the site of infection and into remote tissues was unaffected. Despite higher bacterial counts locally and systemically, STAT4−/− mice had a lower mortality rate than BALB/c controls. In contrast, blockade of IL-12 in BALB/c mice was detrimental to host survival. A blunted serum IL-12 response at 18 h after CLP was exhibited in STAT4−/− mice. These results suggest several critical roles for the STAT4 pathway in the resolution of polymicrobial infections. Additionally, the disparate effects observed with IL-12 blockade and STAT4 deficiency on host survival suggest that IL-12 may activate alternate pathways promoting survival.

LEUKOCYTE-ENDOTHELIUM INTERACTIONS AFTER HEMORRHAGIC SHOCK/REPERFUSION AND CECAL LIGATION/PUNCTURE

Shock ◽  
2006 ◽  
Vol 26 (2) ◽  
pp. 180-186 ◽  
Author(s):  
Naomi Kondo Nakagawa ◽  
Rafael Aydar Nogueira ◽  
Cristiano Jesus Correia ◽  
S??lvia Regina Shiwa ◽  
Jos?? W??lber Miranda Costa Cruz ◽  
...  
Keyword(s):  
Hemorrhagic Shock ◽  
Cecal Ligation ◽  
Cecal Ligation Puncture

scholarly journals Melatonin Alleviates Renal Injury in Mouse Model of Sepsis

2021 ◽  
Vol 12 ◽  
Author(s):  
Liyang Chen ◽  
Zhijian Han ◽  
Zhiguang Shi ◽  
Chao Liu ◽  
Qiulun Lu
Keyword(s):  
Renal Injury ◽  
Oxidant Stress ◽  
Mrna Level ◽  
Cecal Ligation ◽  
Kidney Injury ◽  
Protective Role ◽  
Cecal Ligation Puncture ◽  
Species Production

Melatonin (N-acetyl-5-methoxytryptamine; MLT) has been shown to have a renal-protective effect against kidney injury. However, the mechanisms underlying the protective role of MLT in sepsis-induced renal injury are yet to be revealed. In this study, MLT alleviated renal dysfunction with the increase of BUN (blood urea nitrogen) and SCR (serum creatinine) and reduction of fibrosis in the CLP (cecal ligation puncture) model. RNA-seq analysis showed that MLT repressed the oxidant stress in response to kidney injury. Our in vitro study showed that MLT suppresses LPS-induced accumulation of ROS (reactive oxygen species) production via SOD2 downregulation and Nox4 upregulation in HK-2 cells. Furthermore, we found that MLT alleviated the inflammatory response, with the mRNA-level reduction of Il-1α, Il-1β, Mcp-1, and Tgf-β1. Taken together, in evaluating the therapeutic effect of MLT on sepsis-induced acute kidney injury, the results showed that MLT alleviated renal damage by regulating the production of ROS.

scholarly journals Enteral nutrition ameliorates the symptoms of Crohn's disease in mice via activating special pro-resolving mediators through innate lymphoid cells

2021 ◽  
pp. 175342592110570
Author(s):  
Di Zhao ◽  
Bo Yang ◽  
Chen Ye ◽  
Shaoyi Zhang ◽  
Xiaoqiong Lv ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Enteral Nutrition ◽  
Peritoneal Lavage ◽  
Cecal Ligation ◽  
Serum Levels ◽  
Lymphoid Cells ◽  
Inflammatory Reactions ◽  
Tnf Α ◽  
Hematoxylin Eosin

Crohn's disease activates the inflammatory reactions to induce intestinal disorders. Enteral nutrition (EN) could exert general immunomodulatory effects. Cecal ligation and perforation (CLP) surgery was utilized to establish Crohn's disease mice models. Survival analysis, hematoxylin-eosin staining, flow cytometry, ELISA, Western blot and liquid chromatography-tandem MS were applied. Baicalein was added to inhibit lipoxygenases. The survival rate was restored and inflammatory injury, exudate neutrophils in peritoneal lavage and serum levels of IL-6 and TNF-α were ameliorated by EN treatment as compared with CLP treatment. EN also increased ILC-3 content, 5/15-LOX level and RvD1-RvD5 in peritoneal lavage. Baicalein reversed all the detected effects of EN except ILC-3 content. EN could activate special pro-resolving mediators (SPMs) through ILCs to mitigate injuries of Crohn's disease.

scholarly journals Granzyme A inhibition reduces inflammation and increases survival during abdominal sepsis

2020 ◽  
Author(s):  
Marcela Garzón-Tituaña ◽  
José L Sierra-Monzón ◽  
Laura Comas ◽  
Llipsy Santiago ◽  
Tatiana Khaliulina-Ushakova ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Peritoneal Lavage ◽  
Therapeutic Potential ◽  
Bacterial Load ◽  
Cecal Ligation ◽  
Lavage Fluid ◽  
Abdominal Sepsis ◽  
Granzyme A ◽  
Severe Pathology ◽  
Deficient Mice

ABSTRACTSepsis is a serious syndrome characterised by a dysregulated systemic inflammatory response. Here we have analysed the role and the therapeutic potential of Granzyme A (GzmA) in the pathogenesis of peritoneal sepsis using the Cecal Ligation and Puncture (CLP) polymicrobial sepsis model and samples from humans undergoing abdominal sepsis.Elevated GzmA was observed in serum from patients with abdominal sepsis suggesting that GzmA plays an important role in this pathology. In the CLP model GzmA deficient mice, or WT mice treated with an extracellular GzmA inhibitor, showed increased survival, which correlated with a reduction in proinflammatory markers in both serum and peritoneal lavage fluid. GzmA deficiency did not influence bacterial load in blood and spleen indicating that GzmA has no role in bacterial control. Mechanistically, we found that extracellular active GzmA acts as a proinflammatory mediator in macrophages by inducing the TLR4-dependent expression of IL-6 and TNFα.Our findings implicate GzmA as a key regulator of the inflammatory response during abdominal sepsis, and suggest that it could be targeted for treatment of this severe pathology.

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