Cytotoxic Activities of Phytochemical Components from Ethanol Extract of Ajwa Date on Human Hepatoma Cancer Cells in Vitro

Sarcopoterium spinosum (L.) is a medicinal plant traditionally used for the treatment of various diseases including cancer in the Near- and Middle East. The fractions and constituents of the ethanol extract of S. spinosum were screened for in vitro cytotoxic activities on Jurkat (acute T-lymphoblastic leukemia), HeLa (cervical adenocarcinoma), MCF-7 (mammary gland adenocarcinoma), Caco-2 (human colorectal adenocarcinoma), and MDA-MB-231 (mammary gland adenocarcinoma) cell lines using the MTT (3-(dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The ethanol extract was subsequently re-extracted with ethyl acetate and in its sub-fraction obtained by column chromatography three compounds (stachydrine, benzalkonium chloride and rutine) were the first time identified by nuclear magnetic resonance (NMR) analyses. The most active subfraction showed cytotoxic activity against HeLa, MCF-7, and Caco-2 cell lines. The three compounds mentioned, as standards of high-performance liquid chromatography (HPLC) quality, were studied individually and in combination. Cytotoxic activity observed might be due to the presence of benzalkonium chloride and rutin. Benzalkonium chloride showed the strongest growth suppression effect against HeLa cells (IC50 8.10−7 M) and MCF-7 cells (IC50 5.10−6 M). The mixture of stachydrine and benzalkonium chloride allowed a synergistic cytotoxic effect against all tested cancer and normal cells to be obtained. Anti-cancer activity of the plant extract of S. spinosum remains under-investigated, so this research describes how the three major compounds identified in the ethyl acetate extract can exert a significant dose dependent in vitro cytotoxicity.

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Rhodium(i) N-heterocyclic carbene complexes: synthesis and cytotoxic properties

New Journal of Chemistry ◽

10.1039/d1nj00144b ◽

2021 ◽

Vol 45 (11) ◽

pp. 5176-5183

Author(s):

Ichraf Slimani ◽

Serap Şahin-Bölükbaşı ◽

Mustafa Ulu ◽

Enes Evren ◽

Nevin Gürbüz ◽

...

Keyword(s):

Cancer Cells ◽

Mtt Assay ◽

Incubation Time ◽

Human Cancer ◽

Cytotoxic Activities ◽

Carbene Complexes ◽

Human Cancer Cells ◽

Benzimidazolium Salts ◽

Ht 29

A series of benzimidazolium salts and their [RhCl(NHC)(COD)] complexes were synthesized. All compounds were screened for in vitro cytotoxic activities against a panel of human cancer cells (HT-29 colon, Ishikawa endometrial, U-87 glioblastoma) using the MTT assay for 48 h incubation time.

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Experimental Study on the Effect of Parnassia Ethanol Extract in Inhibiting the Proliferation of Human Breast Cancer Cells in Vitro

Pharmacy Information ◽

10.12677/pi.2016.54013 ◽

2016 ◽

Vol 05 (04) ◽

pp. 73-77

Author(s):

颖辛

Keyword(s):

Breast Cancer ◽

Experimental Study ◽

Cancer Cells ◽

Human Breast Cancer ◽

Breast Cancer Cells ◽

Ethanol Extract ◽

Human Breast Cancer Cells ◽

Human Breast

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Anticancer Potential of an Ethanol Extract of Saussurea Involucrata against Hepatic Cancer Cells in vitro

Asian Pacific Journal of Cancer Prevention ◽

10.7314/apjcp.2014.15.18.7527 ◽

2014 ◽

Vol 15 (18) ◽

pp. 7527-7532 ◽

Cited By ~ 8

Author(s):

Munkhzaya Byambaragchaa ◽

Joseph Dela Cruz ◽

Altantsetseg Kh ◽

Seong-Gu Hwang

Keyword(s):

Cancer Cells ◽

Ethanol Extract ◽

Hepatic Cancer ◽

Saussurea Involucrata

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Composition and in-vitro CytotoxicActivities of the Leaf Essential Oil of Beilschmiedia erythrophloia from Taiwan

Natural Product Communications ◽

10.1177/1934578x1300800135 ◽

2013 ◽

Vol 8 (1) ◽

pp. 1934578X1300800

Author(s):

Yu-Chang Su ◽

Chen-Lung Ho

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Cytotoxic Activity ◽

Cancer Cells ◽

Cytotoxic Activities ◽

Anticancer Activities ◽

Leaf Essential Oil ◽

Main Components ◽

Type Apparatus

This study investigated the chemical composition and in-vitro cytotoxic activities of the essential oil isolated from the leaf of Beilschmiedia erythrophloia. The essential oil was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. Fifty-five compounds were identified, representing 100% of the oil. The main components identified were β-caryophyllene (22.6%), α-humulene (21.9%), terpinen-4-ol (5.3%), cis-β-ocimene (5.1%), sabinene (5.0%) and limonene (4.5%). The anticancer activities of oil were evaluated. The results showed that the oil exhibited cytotoxic activity against human oral, liver, lung, colon, melanoma, and leukemic cancer cells.

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In vivo Neurological, Analgesic and In vitro Antioxidant and Cytotoxic Activities of Ethanolic Extract of Leaf and Stem Bark of Wedelia chinensis

Bangladesh Pharmaceutical Journal ◽

10.3329/bpj.v22i1.40021 ◽

2019 ◽

Vol 22 (1) ◽

pp. 18-26

Author(s):

Sayema Khanum ◽

Md Shahid Sarwar ◽

Mohammad Safiqul Islam

Keyword(s):

Body Weight ◽

Oral Administration ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Pharmaceutical Journal ◽

Stem Bark ◽

Cytotoxic Activities

Wedelia chinensis is a widely used anti-inflammatory and hepatoprotective medicinal plant in Bangladesh. In this study, analgesic, neurological, antioxidant and cytotoxic activities of the ethanolic extract of leaf and stem bark of W. chinensis were investigated. Oral administration of the ethanolic extract of W. chinensis (200- and 300-mg/kg body weight) was investigated on animal model for neurological activity using open field test and hole cross test. Acetic acid induced writhing method was used to assess the analgesic activity. DPPH (1,1-diphenyl, 2-picryl hydrazyl) radical scavenging assay was used for determining the antioxidant activity, while brine shrimp lethality bioassay was used for investigating cytotoxicity. The ethanol extract of the plant produced significant reduction (P<0.05) of locomotion in both doses (200- and 300-mg/kg body weight) indicating pronounced neurological activity. Oral administration of alcoholic leaves and stem extracts significantly (p < 0.05) inhibited writhing response in mice. The percentage of scavenging of DPPH free radical was found to be concentration dependent with IC50 value of 44.10 ± 0.65 and 38.96 ± 0.50 μg/ml for leaves and stem extracts, respectively. Our findings indicate that W. chinensis may be a source of natural antioxidant with potent analgesic, neurological and cytotoxic activities. Bangladesh Pharmaceutical Journal 22(1): 18-26, 2019

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Ethanol extract of baked Gardeniae Fructus exhibits in vitro and in vivo anti-metastatic and anti-angiogenic activities in malignant cancer cells: Role of suppression of the NF-κB and HIF-1α pathways

International Journal of Oncology ◽

10.3892/ijo.2016.3742 ◽

2016 ◽

Vol 49 (6) ◽

pp. 2377-2386 ◽

Cited By ~ 10

Author(s):

Minju Im ◽

Aeyung Kim ◽

Jin Yeul Ma

Keyword(s):

Cancer Cells ◽

Ethanol Extract

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In Vitro Tracing of Cytotoxic Compounds in Jarak Cina Stem Bark (Jatropha Multifida Linn.)

Eksakta Jurnal Ilmu-Ilmu MIPA ◽

10.20885/eksakta.vol1.iss1.art2 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Sista Werdyani ◽

Annisa Fitria ◽

Sari Rakhmawati

Keyword(s):

Ethyl Acetate ◽

Cytotoxic Activity ◽

Cancer Cells ◽

Cancer Cell ◽

Ethyl Acetate Extract ◽

Soxhlet Extraction ◽

Ethanol Extract ◽

Jatropha Multifida ◽

Mcf 7

Cancer remains one of the diseases with increasing number of sufferers, but research on compounds that act as anti-cancer is also ongoing. Terpenoids have been known as a compound that can inhibit the proliferation of cancer cells. One of the medical plants that produce terpenoids is Jarak cina (Jatropha multifida Linn.). Therefore, the possibility of Jarak cina (Jatropha multifida Linn.) to have an cytotoxic activity on cancer cell proliferation is reasonably high. This study was conducted to determine the cytotoxic activity of Jarak cina (Jatropha multifida Linn.) bark extracts against cancer cell MCF-7. Jarak cina bark was extracted using the multilevel soxhlet extraction method with n-hexane, ethyl acetate, and ethanol as the solvents. All the three extracts were then tested against MCF-7 cancer cells using MTT (3-(4,5-dimethylthiazol-2-yl) - 2,5-diphenyltetrazolium bromide) method. Data analysis was performed for IC50 (ppm) parameter. The results showed that the IC50 of n-hexane extract was 313.21 ppm, while the ethyl acetate extract reached 258.38 ppm of IC50, and the IC50 of ethanol extract was 418.51 ppm. The highest potential of cytotoxicity was found in the ethyl acetate extract, so further testing would be required to optimize the proliferation inhibitory activity.

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