Cytotoxicity Effect of the Pericarp Extracts of Garcinia forbesii King on MCF-7 Breast Cancer and HepG2 Liver Cancer Cell Lines

Background: Breast cancer is the most commonly diagnosed cancer and the second leading cause of cancer deaths among Canadian women. Cancer management through changes in lifestyle, such as increased intake of foods rich in dietary flavonoids, have been shown to decrease the risk associated with breast, liver, colorectal, and upper-digestive cancers in epidemiologic studies. Onions are high in flavonoid content and one of the most common vegetables. Additionally, onions are used in most Canadian cuisines.Methods: We investigated the effect of five prominent Ontario grown onion (Stanley, Ruby Ring, LaSalle, Fortress, and Safrane) extracts on two subtypes of breast cancer cell lines: a triple negative breast cancer line MDA-MB-231 and an ER+ breast cancer line MCF-7.Results: These onion extracts elicited strong anti-proliferative, anti-migratory, and cytotoxic activities on both the cancer cell lines. Flavonoids present in these onion extracts induced apoptosis, cell cycle arrest in the G2/M phase, and a reduction in mitochondrial membrane potential at dose-dependent concentrations. Onion extracts were more effective against MDA-MB-231 compared to the MCF-7 cell line. Conclusion: In this study, we investigated the extracts synthesized from Ontario-grown onion varieties in inducing anti-migratory, cytostatic, and cytotoxic activities in two sub-types of human breast cancer cell lines. Anti-tumor activity of these extracts depends upon the varietal and can be formulated into nutraceuticals and functional foods for the wellbeing of cancer patients. Overall, the results suggest that onion extracts are a good source of flavonoids with anti-cancerous properties.Keywords: onion extracts; flavonoids; anti-proliferative; breast cancer; cytotoxic activity

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Underutilized Mexican Plants: Screening of Antioxidant and Antiproliferative Properties of Mexican Cactus Fruit Juices

Plants ◽

10.3390/plants10020368 ◽

2021 ◽

Vol 10 (2) ◽

pp. 368

Author(s):

Elda M. Melchor Martínez ◽

Luisaldo Sandate-Flores ◽

José Rodríguez-Rodríguez ◽

Magdalena Rostro-Alanis ◽

Lizeth Parra-Arroyo ◽

...

Keyword(s):

Cell Proliferation ◽

Liver Cancer ◽

Cell Line ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

In Vitro Assays ◽

Total Phenolic ◽

Liver Cancer Cell ◽

Antiproliferative Activities

Cacti fruits are known to possess antioxidant and antiproliferative activities among other health benefits. The following paper evaluated the antioxidant capacity and bioactivity of five clarified juices from different cacti fruits (Stenocereus spp., Opuntia spp. and M. geomettizans) on four cancer cell lines as well as one normal cell line. Their antioxidant compositions were measured by three different protocols. Their phenolic compositions were quantified through high performance liquid chromatography and the percentages of cell proliferation of fibroblasts as well as breast, prostate, colorectal, and liver cancer cell lines were evaluated though in vitro assays. The results were further processed by principal component analysis. The clarified juice from M. geomettizans fruit showed the highest concentration of total phenolic compounds and induced cell death in liver and colorectal cancer cells lines as well as fibroblasts. The clarified juice extracted from yellow Opuntia ficus-indica fruit displayed antioxidant activity as well as a selective cytotoxic effect on a liver cancer cell line with no toxic effect on fibroblasts. In conclusion, the work supplies evidence on the antioxidant and antiproliferative activities that cacti juices possess, presenting potential as cancer cell proliferation preventing agents.

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Factors modulating integrin Beta I expression on the breast cancer cell lines MCF-7 and MDA-MB-231

Breast Cancer Research ◽

10.1186/bcr157 ◽

2000 ◽

Vol 2 (S1) ◽

Author(s):

CJ Pogson ◽

CMW Chan ◽

L-A Martin ◽

GPH Gui ◽

M Dowsett

Keyword(s):

Breast Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines ◽

Mcf 7

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In vitro investigation of cytotoxic and antioxidative activities of Ardisia crispa against breast cancer cell lines, MCF-7 and MDA-MB-231

BMC Complementary and Alternative Medicine ◽

10.1186/s12906-018-2153-5 ◽

2018 ◽

Vol 18 (1) ◽

Cited By ~ 6

Author(s):

Muhammad Luqman Nordin ◽

Arifah Abdul Kadir ◽

Zainul Amiruddin Zakaria ◽

Rasedee Abdullah ◽

Muhammad Nazrul Hakim Abdullah

Keyword(s):

Breast Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines ◽

In Vitro Investigation ◽

Ardisia Crispa ◽

Mcf 7

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Replacement of hydrochloride in metformin hydrochloride with caprylic acid to investigate its effects on MCF-7 and MDA-MB-231 breast cancer cell lines

Life Sciences ◽

10.1016/j.lfs.2022.120308 ◽

2022 ◽

pp. 120308

Author(s):

Batool Mousavi-Koodehi ◽

Leila Darzi ◽

Majid Sadeghizadeh ◽

Farhood Najafi ◽

Mehdi Forouzandeh-Moghdam

Keyword(s):

Breast Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Caprylic Acid ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines ◽

Metformin Hydrochloride ◽

Mcf 7

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Specific Inhibition of the Redox Activity of Ape1/Ref-1 by E3330 Blocks Tnf-Α-Induced Activation of Il-8 Production in Liver Cancer Cell Lines

PLoS ONE ◽

10.1371/journal.pone.0070909 ◽

2013 ◽

Vol 8 (8) ◽

pp. e70909 ◽

Cited By ~ 24

Author(s):

Laura Cesaratto ◽

Erika Codarin ◽

Carlo Vascotto ◽

Antonio Leonardi ◽

Mark R. Kelley ◽

...

Keyword(s):

Liver Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Redox Activity ◽

Specific Inhibition ◽

Liver Cancer Cell ◽

Tnf Α

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Thymoquinone-Loaded Nanostructured Lipid Carrier Exhibited Cytotoxicity towards Breast Cancer Cell Lines (MDA-MB-231 and MCF-7) and Cervical Cancer Cell Lines (HeLa and SiHa)

BioMed Research International ◽

10.1155/2015/263131 ◽

2015 ◽

Vol 2015 ◽

pp. 1-10 ◽

Cited By ~ 28

Author(s):

Wei Keat Ng ◽

Latifah Saiful Yazan ◽

Li Hua Yap ◽

Wan Abd Ghani Wan Nor Hafiza ◽

Chee Wun How ◽

...

Keyword(s):

Breast Cancer ◽

Cell Cycle ◽

Cervical Cancer ◽

Cell Cycle Arrest ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Nanostructured Lipid Carrier ◽

Cycle Arrest ◽

Mcf 7

Thymoquinone (TQ) has been shown to exhibit antitumor properties. Thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) was developed to improve the bioavailability and cytotoxicity of TQ. This study was conducted to determine the cytotoxic effects of TQ-NLC on breast cancer (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa). TQ-NLC was prepared by applying the hot high pressure homogenization technique. The mean particle size of TQ-NLC was 35.66 ± 0.1235 nm with a narrow polydispersity index (PDI) lower than 0.25. The zeta potential of TQ-NLC was greater than −30 mV. Polysorbate 80 helps to increase the stability of TQ-NLC. Differential scanning calorimetry showed that TQ-NLC has a melting point of 56.73°C, which is lower than that of the bulk material. The encapsulation efficiency of TQ in TQ-NLC was 97.63 ± 0.1798% as determined by HPLC analysis. TQ-NLC exhibited antiproliferative activity towards all the cell lines in a dose-dependent manner which was most cytotoxic towards MDA-MB-231 cells. Cell shrinkage was noted following treatment of MDA-MB-231 cells with TQ-NLC with an increase of apoptotic cell population (P<0.05). TQ-NLC also induced cell cycle arrest. TQ-NLC was most cytotoxic towards MDA-MB-231 cells. It induced apoptosis and cell cycle arrest in the cells.

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The role of miRNAs 34a, 146a, 320a and 542 in the synergistic anticancer effects of methyl 2-(5-fluoro-2-hydroxyphenyl)-1H- benzo[d]imidazole-5-carboxylate (MBIC) with doxorubicin in breast cancer cells

PeerJ ◽

10.7717/peerj.5577 ◽

2018 ◽

Vol 6 ◽

pp. e5577 ◽

Cited By ~ 4

Author(s):

Mohadeseh Hasanpourghadi ◽

Nazia Abdul Majid ◽

Mohd Rais Mustafa

Keyword(s):

Breast Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Breast Cancer Cells ◽

Cancer Cell Lines ◽

Human Breast Cancer Cell ◽

Breast Cancer Cell Lines ◽

Human Breast ◽

Mcf 7

Combination Index (CI) analysis suggested that MBIC and doxorubicin synergistically inhibited up to 97% of cell proliferation in ER+/PR+MCF-7 and triple negative MDA-MB-231 breast cancer cell lines. Moreover, treatment of the breast cancer cells with the combined drugs resulted in lower IC50 values in contrast to the individual drug treatment. Small noncoding microRNAs (miRNA) may function as non-mutational gene regulators at post-transcriptional level of protein synthesis. In the present study, the effect of the combined treatment of MBIC and doxorubicin on the expression level of several miRNAs including miR-34a, miR-146a, miR-320a and miR-542 were evaluated in MCF-7 and MDA-MB-231 breast cancer cell lines. These miRNAs have the potential to alter the protein level of survivin, the anti-apoptotic protein and reduce the metastatic activity in human breast cancer cell lines by interfering with the nuclear accumulation of NF-κB. Our results demonstrated the several fold changes in expression of miRNAs, which is drug and cell line dependent. This finding demonstrated a functional synergistic network between miR-34a, miR-320a and miR-542 that are negatively involved in post-transcriptional regulation of survivin in MCF-7 cells. While in MDA-MB-231 cells, changes in expression level of miR-146a was correlated with inhibition of the nuclear translocation of NF-κB. The overall result suggested that alteration in protein level and location of survivin and NF-κB by miR-34a, miR-320a, miR-146a and miR-542, remarkably influenced the synergistic enhancement of combined MBIC and doxorubicin in treatment of aggressive and less aggressive human breast cancer cell lines.

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