scholarly journals Extrachromosomal DNA Length and Antibiograms of Staphylococcus aureus and Pseudomonas aeruginosa Isolated from Tears of HIV/AIDS Patients after Curing with Sodium Dodecyl Sulphate

2011 ◽  
Vol 4 (1) ◽  
Author(s):  
B. O. Ajayi ◽  
F. D. Otajevwo
1982 ◽  
Vol 152 (2) ◽  
pp. 687-691
Author(s):  
T H Watts ◽  
E A Worobec ◽  
W Paranchych

The proteins of purified inner and outer membranes obtained from Pseudomonas aeruginosa strains PAK and PAK/2Pfs were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to nitrocellulose, and treated with antiserum raised against pure pili. Bound antipilus antibodies were visualized by reaction with 125I-labeled protein A from Staphylococcus aureus. The results showed that there are pools of pilin in both the inner and outer membranes of P. aeruginosa and that the pool size in the multipiliated strain is comparable with that of the wild-type strain.


2012 ◽  
Vol 169 (2) ◽  
pp. 418-430 ◽  
Author(s):  
Sharrel Rebello ◽  
Aju K. Asok ◽  
Sunil V. Joseph ◽  
Biljo V. Joseph ◽  
Leny Jose ◽  
...  

1975 ◽  
Vol 149 (2) ◽  
pp. 397-401 ◽  
Author(s):  
R Virden ◽  
A F Bristow ◽  
R H Pain

1. The penicillinase-catalysed hydrolysis of quinacillin was quenched by addition of 5 m-guanidinium chloride or 1% (w/v) sodium dodecyl sulphate, and the quenched reaction mixture was dialysed exhaustively against solutions of the denaturant. 2. Irreversibly bound quinacillin was shown by titration with HgCl2 to be covalently attached to the protein by the β-lactam carboxyl group. 3. The derivative was found to be stable over the pH range 3.5-8.5. 4. Chymotryptic hydrolysis of the product and subsequent fractionation showed that quinacillin was bound to one or possibly two peptides.


2020 ◽  
Vol 37 ◽  
Author(s):  
Michel Kengne ◽  
Hermann Brice Nkuinzeu Fotie ◽  
Julius Mbekem Nwobegahay ◽  
Patrick Njukeng Achiangia ◽  
Ublad Tamoufe ◽  
...  

2017 ◽  
Vol 1 (3) ◽  
pp. 8
Author(s):  
Veronika Hutabarat ◽  
Hotmarida Silalahi

Abstrak.Pseudomonas aeruginosa merupakan patogen utama bagi manusia yang disebut patogen oportunistik, bakteri ini menjadi problema serius pada pasien rumah sakit yang menangani pasien AIDS yang mengalami penurunan sistem kekebalan akibat perkembangan virus.Tujuan penelitian ini adalah untuk mengetahui pengaruh rawat gabung pasien HIV / AIDS dengan infeksi Pseudomonas aeruginosa terhadap resiko transmisi ke pasien lain di Ruang Dahlia II RSPI Sulianti Saroso Jakarta. Penelitin ini merupakan penelitian pre experimental dengan pre and posttest without control desaign. Sampel dalam penelitian ini adalah pasien HIV/AIDS dewasa yang terinfeksi dan tidak terinfeksi Pseudomonas aeruginosa sebanyak 50 responden yang diambil dengan teknik purposive sampling. Dari 60 pasien didominasi oleh kelompok usia 31-40 Tahun yaitu sebanyak 29 (48.3%), jenis jenis kelamin laki–laki yaitu sebanyak 40 (67 %), tingkat kesadaran compos mentis yaitu sebanyak 50 (83.3 %), jumlah CD4 < 50 yaitu sebanyak 44 (73 %), mikroorganime hasil kultur I didominasi oleh jamur sebanyak 22 (36.7%), mikroorganisme hasil kultur II didominasi oleh Klebsiella pnumonia sebanyak 19 (31.7%). Infeksi oportunistik pasien yaitu 56 (50%) Tb Paru. Hasil penelitian menunjukkan bahwa tidak ada perbedaan transmisi infeksi Pseudomonas aeruginosa sebelum dan sesudah rawat gabung dengan P value (1.000) > (0,05). Diharapkan rawat gabung pasien memperhatikan universal precaution.Abstract.Pseudomonas aeruginosa is main pathogenic for humans called pathogenic opportunistic, this bacteria is serious problem on hospital patients who handles AIDS patients have experienced decline immune systems due to the development of the virus.The purpose of this research is to know the effect of join care of HIV/AIDS patients with Pseudomonas aeruginosa infectious against transmission risk to other patients in the dahlia II ward at hospital of infectious diseases Prof. Dr. Sulianti Saroso in Jakarta.This research is the pre experimental research with pre and post-test without control design. The samples in this research were HIV/AIDS adult patients whom infected and not infected by Pseudomonas aeruginosa as many as 50 respondents taken by using purposive sampling techniques. From 60 patients whom dominated by age group 31-40 years old as many as 29 ( 48.3 % ), the male gender were as many as 40 (67 %), the level of awareness is compos mentis that were as many as 50 ( 83.3 % ), amount of CD4 < 50 namely as many as 44 (73 %), the results of the culture of microorganis that dominated by fungi as many as 22 (36.7%), the results of the culture of microorganisms II that dominated by Klebsiella pnumonia were as many as 19 (31.7 %). Patients of opportinistic infectious were as many as 56 (50%) pulmonology tuberculosis. An opportunistic infection of patients that is 56 (50 percent) pulmonary tuberculosis.The results of research stated that there is no the difference in the transmission of Pseudomonas aeruginosa infections before and after join care with P value (1.000) > (0,05). It is expected to join care of patients more focus and notice on universal precaution.


2017 ◽  
Vol 76 (8) ◽  
pp. 2167-2176 ◽  
Author(s):  
P. S. Ambily ◽  
Sharrel Rebello ◽  
K. Jayachandran ◽  
M. S. Jisha

Anionic surfactants like sodium dodecyl sulphate (SDS), due to its extensive disposal to water bodies cause detrimental effects to the ecosystem. Among the various attempts to reduce the after effects of these toxicants, microbial induced bioremediation serves as a promising strategy. The current study aimed to develop a three stage bioreactor to remediate anionic surfactants in wastewater using effective bacterial isolates. Screening of effective SDS biodegraders led to isolation of Pseudomonas aeruginosa (MTCC 10311). Treatment of synthetic effluent with an immobilized packed bed reactor at a flow rate of 5 mL h−1 resulted in 81 ± 2% SDS eliminations and 70 ± 1% reduction in chemical oxygen demand (COD) in five cycles (6 h per cycle). The hydraulic retention time of the reactor was found to be 6 h. Combinatorial usage of a three stage bioreactor, involving aeration, adsorption with low cost scrap rubber granules and treatment with immobilized Pseudomonas aeruginosa, successfully reduced SDS concentrations and COD of wastewater to 99.8 ± 0.1% and 99 ± 1%, respectively, in 18 h by continuous treatment. Half-life of the three stage bioreactor was 72 h. In addition to reducing the surfactant concentrations, this novel bioreactor could resolve the surfactant associated foaming problems in treatment plants, which make it more unique.


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