scholarly journals Language, Media and Ideology: CDA of an Animation, “The Donkey King”

2020 ◽  
Vol 10 (5) ◽  
pp. 366
Author(s):  
Afshan Quyyum ◽  
Sikandar Ali ◽  
Junaid Akash
Keyword(s):  
Critical Thinking ◽  
Qualitative Method ◽  
Three Dimensional ◽  
False Consciousness ◽  
Reality Construction ◽  
The Common ◽  
The Masses ◽  
Random Sampling Approach ◽  
Insight Into

The present study has looked into the hidden realities and ideologies in an animation “The Donkey King” released on 13th October 2018, by using the English subtitles. This animation seems to be a reminiscent of the Orwellian plot and is a phenomenal depiction of the contemporary political scenario in Pakistan. Realities can be constructed, ideologies can be created and false consciousness can be spread among the common masses by unknown and unseen powers of the society. The research has explicated the hidden realities and ideologies through CDA three-dimensional framework proposed by Norman Fairclough (1989, 1992a, 1993, 1995). Qualitative method with convenient random sampling approach in chronological order has been utilized to carry out the research. CDA is a combination of politics and language use and the study provides a deep insight into the role of cinema, in reality construction. The study concludes that cinema is a powerful tool capable enough to create realities and ideologies among the masses. The study also contains message for all ages that the real king is the savior of the nation no matter which creed or cast he belongs to. The study is significant to bring changes in the society and recommends some practical and useful suggestions to the readers, leaders, viewers of cinema and for the upcoming researchers who can bring a positive change in the society. The main significance of the study lies in creating awareness in the masses for the welfare of a free and open society. Additionally, the current study suggests that critical thinking is crucial for viewers and readers; critical thinking would help them not to easily get triggered by the issues intended by the powerful institutions, in which ideology and power keep important influences. This study filled many gaps and persisted with numerous gaps for upcoming researchers. It is expected that the current study is going to serve a useful reference resource for the researchers in understanding the study about ideology and power in media discourse.

scholarly journals The distribution of myosin II in Dictyostelium discoideum slug cells.

1991 ◽  
Vol 115 (5) ◽  
pp. 1267-1274 ◽  
Author(s):  
S Eliott ◽  
P H Vardy ◽  
K L Williams
Keyword(s):  
Cell Motility ◽  
Dictyostelium Discoideum ◽  
Immunofluorescence Microscopy ◽  
Molecular Genetic ◽  
Myosin Ii ◽  
Three Dimensional ◽  
Homogeneous Distribution ◽  
Multicellular Development ◽  
Insight Into

While the role of myosin II in muscle contraction has been well characterized, less is known about the role of myosin II in non-muscle cells. Recent molecular genetic experiments on Dictyostelium discoideum show that myosin II is necessary for cytokinesis and multicellular development. Here we use immunofluorescence microscopy with monoclonal and polyclonal antimyosin antibodies to visualize myosin II in cells of the multicellular D. discoideum slug. A subpopulation of peripheral and anterior cells label brightly with antimyosin II antibodies, and many of these cells display a polarized intracellular distribution of myosin II. Other cells in the slug label less brightly and their cytoplasm displays a more homogeneous distribution of myosin II. These results provide insight into cell motility within a three-dimensional tissue and they are discussed in relation to the possible roles of myosin II in multicellular development.

scholarly journals Strategi Kodim 1703/Manokwari Dalam Penanganan Konflik Sosial di Provinsi Papua Barat

2021 ◽  
Vol 2 (1) ◽  
pp. 149-158
Author(s):  
Uchaimid Biridlo'i Robby ◽  
Dedi Akhiruddin
Keyword(s):  
Qualitative Method ◽  
Social Conflict ◽  
Social Conflicts ◽  
Conflict Situations ◽  
West Papua ◽  
Conflict Handling ◽  
The Republic ◽  
Papua Barat ◽  
The Masses

The level of pluralism of the Indonesian nation, which has a variety of religions, cultures, languages ​​and ethnicities, makes the Unitary State of the Republic of Indonesia a heterogeneous country, so it is common for friction to occur and cause social conflict by directing the masses to act irrationally. Conflict situations tend to be easily exploited by those who try to take advantage of them. Conflict parties have different understandings about the problems at hand. From this explanation explicitly, the importance of the involvement of Kodim 1703 / Manokwari of West Papua Province in dealing with social conflicts cannot only be borne by the handling of conflicts that have occurred but is an initial form of a persuasive approach to all elements of society. needed. This study aims to identify and analyze the strategy of Kodim 1703 / Manokwari of West Papua Province in handling social conflicts based on Law of the Republic of Indonesia Number 7 of 2012 in handling social conflicts in Indonesia, especially West Papua Province. The method used by the writer is descriptive qualitative method. The data obtained came from interviews with informants who were directly involved in the field during the handling of social conflicts in Manokwari. Based on the research results, it can be concluded as follows: (1) The role of Kodim 1703 / Manokwari in handling social conflicts in Manokwari City is limited by Law Number 7 of 2012 concerning Social Conflict Handling so that it is not optimal by involving all levels of society so that no one feels left out or ignored.   Keywords: Management Strategic and Social Conflict

scholarly journals Role of Computational Methods in Going beyond X-ray Crystallography to Explore Protein Structure and Dynamics

2018 ◽  
Vol 19 (11) ◽  
pp. 3401 ◽  
Author(s):  
Ashutosh Srivastava ◽  
Tetsuro Nagai ◽  
Arpita Srivastava ◽  
Osamu Miyashita ◽  
Florence Tama
Keyword(s):  
Protein Dynamics ◽  
Computational Methods ◽  
Protein Structures ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
X Ray ◽  
X Ray Crystallography ◽  
Insight Into

Protein structural biology came a long way since the determination of the first three-dimensional structure of myoglobin about six decades ago. Across this period, X-ray crystallography was the most important experimental method for gaining atomic-resolution insight into protein structures. However, as the role of dynamics gained importance in the function of proteins, the limitations of X-ray crystallography in not being able to capture dynamics came to the forefront. Computational methods proved to be immensely successful in understanding protein dynamics in solution, and they continue to improve in terms of both the scale and the types of systems that can be studied. In this review, we briefly discuss the limitations of X-ray crystallography in studying protein dynamics, and then provide an overview of different computational methods that are instrumental in understanding the dynamics of proteins and biomacromolecular complexes.

scholarly journals Architecturally complex O-glycopeptidases are customized for mucin recognition and hydrolysis

2021 ◽  
Vol 118 (10) ◽  
pp. e2019220118
Author(s):  
Benjamin Pluvinage ◽  
Elizabeth Ficko-Blean ◽  
Ilit Noach ◽  
Christopher Stuart ◽  
Nicole Thompson ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Peptide Bond ◽  
Molecular Organization ◽  
Dimensional Structure ◽  
Carbohydrate Binding ◽  
Functional Roles ◽  
Protein Substrates ◽  
Individual Domain ◽  
Insight Into

A challenge faced by peptidases is the recognition of highly diverse substrates. A feature of some peptidase families is the capacity to specifically use post-translationally added glycans present on their protein substrates as a recognition determinant. This is ultimately critical to enabling peptide bond hydrolysis. This class of enzyme is also frequently large and architecturally sophisticated. However, the molecular details underpinning glycan recognition by these O-glycopeptidases, the importance of these interactions, and the functional roles of their ancillary domains remain unclear. Here, using the Clostridium perfringens ZmpA, ZmpB, and ZmpC M60 peptidases as model proteins, we provide structural and functional insight into how these intricate proteins recognize glycans as part of catalytic and noncatalytic substrate recognition. Structural, kinetic, and mutagenic analyses support the key role of glycan recognition within the M60 domain catalytic site, though they point to ZmpA as an apparently inactive enzyme. Wider examination of the Zmp domain content reveals noncatalytic carbohydrate binding as a feature of these proteins. The complete three-dimensional structure of ZmpB provides rare insight into the overall molecular organization of a highly multimodular enzyme and reveals how the interplay of individual domain function may influence biological activity. O-glycopeptidases frequently occur in host-adapted microbes that inhabit or attack mucus layers. Therefore, we anticipate that these results will be fundamental to informing more detailed models of how the glycoproteins that are abundant in mucus are destroyed as part of pathogenic processes or liberated as energy sources during normal commensal lifestyles.

Pituitary blood flow

1982 ◽  
Vol 243 (6) ◽  
pp. E427-E442 ◽  
Author(s):  
R. B. Page
Keyword(s):  
Blood Flow ◽  
Median Eminence ◽  
Three Dimensional ◽  
Vascular Anatomy ◽  
Flow Regulation ◽  
Organ Blood Flow ◽  
Regional Control ◽  
New Concepts ◽  
Insight Into

The direction of pituitary blood flow, the amount of pituitary blood flow, its regional control, and the role of the median eminence microcirculation are the subjects of this review. Present concepts of pituitary blood flow are focused almost entirely on its direction and arouse from studies of pituitary vascular anatomy performed almost 50 years ago. The development of new anatomic techniques has led to a reappraisal of pituitary angioarchitecture, stimulated physiological studies to clarify the pattern of blood flow within the entire gland, and led to a reappraisal of accepted concepts of directional pituitary blood flow. The availability of techniques to accurately measure organ blood flow has permitted study of pituitary blood flow; and, when combined with knowledge of pituitary anatomy, the application of these techniques promises to provide a means to develop insight into control of the mechanisms by which chemical messengers are delivered to the pituitary to control its function. New anatomic techniques promise to develop new understanding of the three-dimensional arrangement of median eminence microvasculature and yield new concepts of blood flow regulation within the median eminence that can be tested by physiological means.

scholarly journals Exploring the Nature of Desmosomal Cadherin Associations in 3D

2010 ◽  
Vol 2010 ◽  
pp. 1-12 ◽  
Author(s):  
Gethin R. Owen ◽  
David L. Stokes
Keyword(s):  
Electron Tomography ◽  
Three Dimensional ◽  
Direct Consequence ◽  
Desmosomal Cadherins ◽  
Protein Molecules ◽  
Structural Interactions ◽  
Structural Differences ◽  
Physical Principles ◽  
Insight Into

Desmosomes are a complex assembly of protein molecules that mediate adhesion between adjacent cells. Desmosome composition is well established and spatial relationships between components have been identified. Intercellular cell-cell adhesion is created by the interaction of extracellular domains of desmosomal cadherins, namely, desmocollins and desmogleins. High-resolution methods have provided insight into the structural interactions between cadherins. However, there is a lack of understanding about the architecture of the intact desmosomes and the physical principles behind their adhesive strength are unclear. Electron Tomography (ET) studies have offered three-dimensional visual data of desmosomal cadherin associations at molecular resolution. This review discusses the merits of two cadherin association models represented using ET. We discuss the possible role of sample preparation on the structural differences seen between models and the possibility of adaptive changes in the structure as a direct consequence of mechanical stress and stratification.

scholarly journals MicroRNAs: a complex regulatory network drives the acquisition of malignant cell phenotype

2010 ◽  
Vol 17 (1) ◽  
pp. F51-F75 ◽  
Author(s):  
Libero Santarpia ◽  
Milena Nicoloso ◽  
George A Calin
Keyword(s):  
Cancer Biology ◽  
Malignant Cell ◽  
Cell Phenotype ◽  
Endocrine Tumors ◽  
Functional Capabilities ◽  
Self Sufficiency ◽  
Multistep Process ◽  
The Common ◽  
Insight Into

Several lines of evidence indicate that tumorigenesis is a complex multistep process, and that most, if not all, cancers acquire the same set of functional capabilities during development and progression, albeit through various mechanistic strategies. Increasing data show an important role of microRNAs (miRNAs or miRs) in regulating various aspects of cancer biology. This review describes the role of microRNAs during the multiple steps that drive the progressive transformation of normal cells into highly malignant derivatives, outlining the role of microRNAs in regulating the common hallmarks of tumorigenesis: self-sufficiency in growth signals, insensitivity to antigrowth signals, abnormal apoptosis, limitless replicative potential, induction and sustained angiogenesis, and tissue invasion and metastasis. Recent evidence suggests an important role of microRNAs in the regulation of the expression of most genes regulating and coordinating a wide variety of processes in endocrine glands. We will highlight microRNAs of potential relevance to endocrine tumors and hormone-dependent cancers. Through this overview of how microRNAs regulate multiple targets and entire pathways, we will provide insight into the potential to develop new molecular microRNA-targeted therapies for endocrine tumors.

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