Socioeconomic Determinants of Female Development in the Rural North West Province of South Africa

Women engagement and reward systems are vital for rural development as they have a direct consequence on the improvement, equality, as well as accelerating agricultural productivity generally, and increasing food supply in particular at the local and national levels. The study highlighted the contribution of the Award Incentives and Competition (AIC) programme in enhancing women farmers’ economic strength. It also looked at how influence, change and technological know-how could improve women farmers’ participation in development programmes. It provided an overview of the determinants of female entrepreneurship development in rural North West Province using a quantitative survey method approach. One hundred and fifty-six women farmers, who were randomly selected from all districts in the province and different enterprises out of 520 eligible women farmers, participated in the study. Data were analysed using descriptive statistics and inferential (Probit regression) model. Based on empirical data, the Probit regression model estimates suggested that the educational status, creation of temporary and permanent jobs, and extension sources were some of the determinants of entrepreneurial development in the study area. The study recommends that policymakers continue to advocate for education as female farmers need to participate in development programme(s).

SPITFIR(e): A supermaneuverable algorithm for restoring 2D-3D fluorescence images and videos, and background subtraction

While fluorescent microscopy imaging has become the spearhead of modern biology as it is able to generate long-term videos depicting 4D nanoscale cell behaviors, it is still limited by the optical aberrations and the photon budget available in the specimen and to some extend to photo-toxicity. A direct consequence is the necessity to develop flexible and "off-road" algorithms in order to recover structural details and improve spatial resolution, which is critical when pushing the illumination to the low levels in order to limit photo-damages. Moreover, as the processing of very large temporal series of images considerably slows down the analysis, special attention must be paid to the feasibility and scalability of the developed restoration algorithms. To address these specifications, we present a very flexible method designed to restore 2D-3D+Time fluorescent images and subtract undesirable out-of-focus background. We assume that the images are sparse and piece-wise smooth, and are corrupted by mixed Poisson-Gaussian noise. To recover the unknown image, we consider a novel convex and non-quadratic regularizer Sparse Hessian Variation) defined as the mixed norms which gathers image intensity and spatial second-order derivatives. This resulting restoration algorithm named SPITFIR(e) (SParse fIT for Fluorescence Image Restoration) utilizes the primal-dual optimization principle for energy minimization and can be used to process large images acquired with varied fluorescence microscopy modalities. It is nearly parameter-free as the practitioner needs only to specify the amount of desired sparsity (weak, moderate, high). Experimental results in lattice light sheet, stimulated emission depletion, multifocus microscopy, spinning disk confocal, and wide-field microscopy demonstrate the generic ability of the SPITFIR(e) algorithm to efficiently reduce noise and blur, and to subtract undesirable fluorescent background, while avoiding the emergence of deconvolution artifacts.

AGC1 Deficiency: Pathology and Molecular and Cellular Mechanisms of the Disease

AGC1/Aralar/Slc25a12 is the mitochondrial carrier of aspartate-glutamate, the regulatory component of the NADH malate-aspartate shuttle (MAS) that transfers cytosolic redox power to neuronal mitochondria. The deficiency in AGC1/Aralar leads to the human rare disease named “early infantile epileptic encephalopathy 39” (EIEE 39, OMIM # 612949) characterized by epilepsy, hypotonia, arrested psychomotor neurodevelopment, hypo myelination and a drastic drop in brain aspartate (Asp) and N-acetylaspartate (NAA). Current evidence suggest that neurons are the main brain cell type expressing Aralar. However, paradoxically, glial functions such as myelin and Glutamine (Gln) synthesis are markedly impaired in AGC1 deficiency. Herein, we discuss the role of the AGC1/Aralar-MAS pathway in neuronal functions such as Asp and NAA synthesis, lactate use, respiration on glucose, glutamate (Glu) oxidation and other neurometabolic aspects. The possible mechanism triggering the pathophysiological findings in AGC1 deficiency, such as epilepsy and postnatal hypomyelination observed in humans and mice, are also included. Many of these mechanisms arise from findings in the aralar-KO mice model that extensively recapitulate the human disease including the astroglial failure to synthesize Gln and the dopamine (DA) mishandling in the nigrostriatal system. Epilepsy and DA mishandling are a direct consequence of the metabolic defect in neurons due to AGC1/Aralar deficiency. However, the deficits in myelin and Gln synthesis may be a consequence of neuronal affectation or a direct effect of AGC1/Aralar deficiency in glial cells. Further research is needed to clarify this question and delineate the transcellular metabolic fluxes that control brain functions. Finally, we discuss therapeutic approaches successfully used in AGC1-deficient patients and mice.

Perceptions and attitudes of University of Johannesburg chiropractic students toward a blended learning approach and a shift to an e-learning approach necessitated by the COVID-19 pandemic

ABSTRACT Objective This study aimed to explore and compare the perceptions and attitudes of chiropractic students on a blended learning offering in 2019 and a subsequent shift to an e-learning approach in 2020 owing to the COVID-19 pandemic. Methods This was an exploratory descriptive study of 4th-year BHSc chiropractic students enrolled in the Clinical and Applied Biomechanics IV module in 2019 (n = 31) and 2020 (n = 33). The survey used close-ended Likert scale questions collected from 29 July to 14 August 2020. Data were analyzed using frequencies and descriptions, exploratory factor analysis, and reliability. Trends and interrelationships of and between student attitude, satisfaction, social influences, ease of use, accessibility, and effectivity were investigated for each year and compared between successive years' cohorts. Results Students were mostly female (76.6%), aged 20 to 24 years (84.4%). Although both cohorts showed similar positive attitudes, accessibility, and satisfaction levels, the e-learning group showed increased effectivity (p = .016) and ease of use (p = .038) compared with the blended learning cohort. Face-to-face time with the lecturer was shown to be more important to the blended learning cohort (p = .006). Strong correlations were demonstrated in both cohorts between accessibility and satisfaction with attitude, effectivity, and ease of use. Conclusion Findings suggest that students were more receptive to an e-learning approach than they may have been in the past. This may be as a direct consequence of the response to COVID-19, and the adapted offerings of the curriculum.

Robustness of DNA Looping Across Multiple Divisions in Individual Bacteria

DNA looping has emerged as a central paradigm of transcriptional regulation as it is shared across many living systems. One core property of DNA looping-based regulation is its ability to greatly enhance repression or activation of genes with only a few copies of transcriptional regulators. However, this property based on small number of proteins raises the question of the robustness of such a mechanism with respect to the large intracellular perturbations taking place during growth and division of the cell. Here we address the issue of sensitivity to variations of intracellular parameters of gene regulation by DNA looping. We use the lac system as a prototype to experimentally identify the key features of the robustness of DNA looping in growing E. coli cells. Surprisingly, we observe time intervals of tight repression spanning across division events, which can sometimes exceed ten generations. Remarkably, the distribution of such long time intervals exhibits memoryless statistics that is mostly insensitive to repressor concentration, cell division events, and the number of distinct loops accessible to the system. By contrast, gene regulation becomes highly sensitive to these perturbations when DNA looping is absent. Using stochastic simulations, we propose that the robustness to division events of memoryless distributions emerges from the competition between fast, multiple re-binding events of repressors and slow initiation rate of the RNA-polymerase. We argue that fast re-binding events are a direct consequence of DNA looping that ensures robust gene repression across a range of intracellular perturbations.

In a holistic perspective, time is absolute and relativity a direct consequence of the conservation of total energy

We are taught to think that the description of relativistic phenomena requires distorted time and distance. The message of this essay is that, in a holistic perspective, time and distance are universal coordinate quantities, and relativity is a direct consequence of the conservation of energy. Instead of the kinematics/metrics-based approach of the theory of relativity, the dynamic universe (DU) approach starts from the dynamics of space as a whole and expresses relativity in terms of locally available energy instead of locally distorted time and distance. In such an approach, e.g., the frequency of atomic clocks at different states of motion and gravitation is obtained from the quantum mechanical solution of the characteristic frequencies, and the unique status of the velocity of light becomes understood via its linkage to the rest of space. In the kinematic/metrics-based theory of relativity, we postulate the principle of relativity, Lorentz covariance, the equivalence principle, the constancy of the speed of light, and the rest energy of mass objects. The conservation of momentum and energy is honored in local frames of reference, and time and distance are parameters in frame-to-frame observations. In the dynamics-based DU, the whole space is studied as a closed energy system and the energy in local structures is derived conserving the overall energy balance. Any local state of motion and gravitation in space is related, through a system of nested energy frames, to the state of rest in hypothetical homogeneous space, which serves as the universal frame of reference. Relativity of observations appears as a direct consequence of the overall energy balance and the linkage of local to the whole—with time and distance as universal coordinate quantities. DU postulates spherically closed space and zero-energy balance of motion and gravitation. DU does not need the relativity principle or any other postulates of the theory of relativity. Primarily, the theory of relativity is an empirically driven mathematical description of observations, with postulates formulated to support the mathematics. DU relies on mathematics built on the conservation of an overall zero-energy balance as the primary law of nature, which makes DU more like a metaphysically driven theory. Both approaches produce precise predictions. The choice is philosophical—nature is not dependent on the way we describe it.

Effect of different irrigation porotocols on root dentine micro-hardness-An invitro study

IIrrigation solutions used and time of use has a definite effect on the micro hardness & other physical properties of dentin which in turn have direct consequence on the longevity functional performance of root canal treated teeth. To evaluate the effect of different irrigation solutions on micro hardness of root dentin.Forty extracted single rooted lower premolars were used. After instrumentation all the root halves were randomly assigned into 4groups (n=10) and brought in contact with one of the following irrigants for 5 minutes. Group I: 10 ml of 5% Sodium Hypochlorite (NaOCl).Group II: 10 ml of 17% ethylene diamine tetra-acetic acid (EDTA) followed by 10 ml of 5% NaOCl.Group III: 10 ml of 5% NaOCl followed by 10 ml of 2% chlorhexidine digluconate (CHX).Group IV: 10 ml of 5% NaOCL followed by flush of 10 ml distilled water then by 10ml of 2% CHX. Dentin micro hardness was measured at baseline and after treatment to determine the change in micro hardness, using Vickers tester.Data was analyzed using following parametric tests t-test, ANOVA test and Post Hoc test. Group II ie final irrigation with EDTA showed the highest percentage decrease in micro hardness values, followed by group III, then group IV and the lowest was group I. All groups showed a significant difference between each other (P < 0.05), except group III and IV. The coronal third showed the highest percentage decrease with significant difference between apical and middle thirds (P < 0.05).EDTA with NaOCl causes greatest changes in dentine micro hardness, an intermediate flush with normal saline should be given for prevention of precipitation with NaOCl & CHX.

Proving the Lorentz Invariance of the Entropy and the Covariance of Thermodynamics

The standard argument for the Lorentz invariance of the thermodynamic entropy in equilibrium is based on the assumption that it is possible to perform an adiabatic transformation whose only outcome is to accelerate a macroscopic body, keeping its rest mass unchanged. The validity of this assumption constitutes the very foundation of relativistic thermodynamics and needs to be tested in greater detail. We show that, indeed, such a transformation is always possible, at least in principle. The only two assumptions invoked in the proof are that there is at least one inertial reference frame in which the second law of thermodynamics is valid and that the microscopic theory describing the internal dynamics of the body is a field theory, with Lorentz invariant Lagrangian density. The proof makes no reference to the connection between entropy and probabilities and is valid both within classical and quantum physics. To avoid any risk of circular reasoning, we do not postulate that the laws of thermodynamics are the same in every reference frame, but we obtain this fact as a direct consequence of the Lorentz invariance of the entropy.

Physical Activity and Sports Practice in Children and Adolescents at the Time of the COVID-19 Pandemic: Hypothetical Future Scenarios and Preventive Practical Applications

As a logical and direct consequence of the closure of schools and sports facilities, an expected reduction in the practice of physical activity (PA) and sports has been detected in both children and adolescents all over the world. Hence, we analysed the short-term and long-term consequences of the lack/low level of PA and sports activity in this population, which we referred to as primary and secondary risks, respectively, the latter of which have to be considered in hypothetical future scenarios.

Human Trafficking in the Context of Global Migration: Modern Manifestation of De Facto Slavery, Servitude and Forced or Compulsory Labour

In recent years there has been a significant increase in trafficking in human beings as a global phenomenon. COVID-19 pandemic created conditions that increased the number of persons who were vulnerable to human trafficking and disrupted current and planned anti-trafficking initiatives. Human trafficking treats human beings as commodities to be bought and sold and put to forced labour often for lower or no payment. This constitutes a modern form of de facto slavery, servitude and forced or compulsory labour. This article provides an overview of international law on human trafficking and considers response to human trafficking in Africa. It further considers whether diplomats can be held accountable for exploitation of migrant domestic workers in receiving States. It further examines whether diplomatic immunity can be used as a bar to the exercise of jurisdiction by domestic courts and tribunals of a state which hosts the diplomat (the ‘receiving state’) in cases of employment of a trafficked person by a former or serving diplomat. It ends by considering whether trafficked persons should be held to bear individual criminal responsibility for crimes they have committed (or were compelled to commit) in the course, or as a direct consequence, of having been trafficked. Such crimes may include unlawful entry into, presence or residence in another country of transit or destination, working without a work permit, sex work, and use of false identity/false passport.