Antioxidant Activity of Spray-Dried Extracts of Psidium guajava Leaves

Antioxidants from Psidium guajava leaves were extracted with 70% ethanol in water. The extractive solution was concentrated and submitted to spray drying in the presence of technological adjuvants at a proportion of 8 % wet base. Three distinct technological adjuvants were evaluated: β-cyclodextrin (βCD-80), maltodextrin DE10:Aerosil® (MA-80 - 7:1), and maltodextrin DE10:Encapsia®:Aerosil® (MDEA-80 - 5:2:1). The antioxidant activity of the concentrated extract and spray-dried powders was assessed by three antioxidant assays, namely: the2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging, ferric reducing antioxidant power (FRAP), and oxygen radical absorption capacity (ORAC). The 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging capacity was determined previously. The spray-dried powders exhibited strong antioxidant activity (IC50 value = 7.96 to 9.76 μg/mL – DPPH method; 3,125.1 to 3,406.0 μM TE/g dry weight – ABTS method; 4,210 to 4,540 μM FeSO4 E/g – FRAP method; 1,820-2,020 μM TE/g – ORAC method). The technological adjuvants did not significantly interfere with the antioxidant activity of the dried products, regardless the type of antioxidant assay used. The results here reported strongly evidenced that the concentrated and spray-dried extracts of Psidium guajava are rich sources of natural antioxidants with potential application in food, pharmaceutical, and cosmeceutical products.

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Assessment of Antioxidant Activity of Spray Dried Extracts ofPsidium guajavaLeaves by DPPH and Chemiluminescence Inhibition in Human Neutrophils

BioMed Research International ◽

10.1155/2014/382891 ◽

2014 ◽

Vol 2014 ◽

pp. 1-10 ◽

Cited By ~ 6

Author(s):

M. R. V. Fernandes ◽

A. E. C. S. Azzolini ◽

M. L. L. Martinez ◽

C. R. F. Souza ◽

Y. M. Lucisano-Valim ◽

...

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging ◽

Psidium Guajava ◽

Human Neutrophils ◽

Total Phenolic ◽

Dependent Manner ◽

Concentration Dependent Manner ◽

Dpph Method ◽

Solids Content ◽

Spray Dried

This work evaluated the physicochemical properties and antioxidant activity of spray dried extracts (SDE) fromPsidium guajavaL. leaves. Different drying carriers, namely, maltodextrin, colloidal silicon dioxide, Arabic gum, andβ-cyclodextrin at concentrations of 40 and 80% relative to solids content, were added to drying composition. SDE were characterized through determination of the total phenolic, tannins, and flavonoid content. Antioxidant potential of the SDE was assessed by two assays: cellular test that measures the luminol-enhanced chemiluminescence (LumCL) produced by neutrophils stimulated with phorbol myristate acetate (PMA) and the DPPH radical scavenging (DPPH* method). In both assays the antioxidant activity of the SDE occurred in a concentration-dependent manner and showed no toxicity to the cells. Using the CLlummethod, the IC50ranged from 5.42 to 6.50 µg/mL. The IC50of the SDE ranged from 7.96 to 8.11 µg/mL using the DPPH•method.Psidium guajavaSDE presented significant antioxidant activity; thus they show high potential as an active phytopharmaceutical ingredient. Our findings in human neutrophils are pharmacologically relevant since they indicate thatP. guajavaSDE is a potential antioxidant and anti-inflammatory agent in human cells.

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Assessment of antioxidant and antibacterial activities of crude extracts of verbena officinalis Linn root or Atuch (Amharic)

10.31221/osf.io/pzh3s ◽

2019 ◽

Cited By ~ 1

Author(s):

Chem Int

Keyword(s):

Antioxidant Activity ◽

Methanolic Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Dry Weight ◽

Antimicrobial Activities ◽

Total Flavonoid ◽

Root Extract ◽

Antioxidant Power ◽

Crude Extracts

Verbena officinalis Linn is a traditionally known medicinal plant which is used against a number of diseases including inflammatory conditions. In this study its antioxidant activity (reducing powers, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities), ferric reduction activity potential (FRAP), total flavonoid concentration and antimicrobial activities of 80%, 90%, 100% methanol and chloroform extracts of V. officinalis Linn root and 90% and100% methanol leaf extracts were determined. Its antioxidant activity increases with increase in amount of extract (10% to 40%v/v). Total flavonoid content (TFC) varied from 73.32±0.002 mgQE/100g of dry weight (90% methanol) to 42.39±0.032 mgQE/100g dry weight (chloroform), 2,2-diphenyl-1-picrylhydrazyl (DPPH), radical scavenging activity (%) was varied between 87.39% (90% methanol) to 45.57% (chloroform) while Ferric reducing antioxidant power was observed between 372.93±0.04 mgAAE/100 g extract (90% methanol) to 129.41±0.026 mgAAE/100 g chloroform in the root extract. The methanolic extract of the leaf showed less antioxidant activity than the methanolic extract of the root. Crude extracts of V. officinalis root showed various degree of antimicrobial activity towards drug resistance microbial pathogens. Growth inhibition tests against bacterial pathogens demonstrated concentration dependence. Moreover, gram positive bacteria were more susceptible to V. officinalis root extract when compared to gram negative bacteria. In general V. officinalis root and leave extracts possess strong antioxidant and antimicrobial activities.

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Ultrasonic Extraction of Bioactive Compounds From Green Soybean Pods and Application in Green Soybean Milk Antioxidants Fortification

10.21203/rs.3.rs-1048606/v1 ◽

2021 ◽

Author(s):

Noppol Leksawasdi ◽

Siraphat Taesuwan ◽

Trakul Prommajak ◽

Charin Techapun ◽

Rattanaporn Khonchaisri ◽

...

Keyword(s):

Antioxidant Activity ◽

Bioactive Compounds ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Bioactive Compound ◽

Natural Antioxidants ◽

Dependent Manner ◽

Ultrasound Technique ◽

Antioxidant Power ◽

Soybean Milk

Abstract Green soybean (Glycine max L.) pods (GSP) are agro-industrial waste from the production of frozen green soybean and milk. These pods contain natural antioxidants and various bioactive compounds that are still underutilized. Polyphenols and flavonoids in GSP were extracted by ultrasound techniques and used in antioxidant fortification of green soybean milk. The ultrasound extraction that yielded the highest total polyphenol content was 50% amplitude for 10 min, whereas maximum flavonoids content was obtained at 50% amplitude for 15 min. Radical scavenging activity assayed by 2,2-diphenyl-1-picryl-hydrazyl radical (DPPH) and ferric reducing antioxidant power (FRAP) methods did not differ significantly (p>0.05) between the two conditions. Response surface methodology was applied to analyze an optimum ultrasonic-assisted extraction (UAE) condition of these variables. The highest desirability was found to be 50% amplitude with extraction time of 12.5 min. Fortification of the GSP extracts (1-3% v/v) in green soybean milk resulted in higher levels of bioactive compounds and antioxidant activity in a dose-dependent manner. Procyanidins were found to be the main polyphenols in dried GSP which were present at the concentration of 104.1 ± 2.1 mg/g dry sample, respectively. Addition of GSP extracts obtained by using an ultrasound technique to green soybean milk increased its bioactive compound content especially procyanidins as well as its antioxidant activity.

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Antioxidant Activity and Total Phenolic Content of 24 Lamiaceae Species Growing in Iran

Natural Product Communications ◽

10.1177/1934578x1000500219 ◽

2010 ◽

Vol 5 (2) ◽

pp. 1934578X1000500 ◽

Cited By ~ 4

Author(s):

Omidreza Firuzi ◽

Katayoun Javidnia ◽

Maryam Gholami ◽

Mohammad Soltani ◽

Ramin Miri

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging ◽

Dry Weight ◽

Natural Antioxidants ◽

Total Phenolic ◽

Dpph Assay ◽

Antioxidant Power ◽

Phenolic Contents ◽

Methanolic Extracts ◽

Ferric Reducing Antioxidant Power

The antioxidant activities of the methanolic extracts of 9 Salvia species and 15 other Lamiaceae plants growing in Iran were evaluated using ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. FRAP values ranged form 8.5 to 79.0 μM quercetin equivalents/g dry weight, and IC50 values in the DPPH assay from 115.7 to 1350.2 μg dry weight/mL. Salvia species showed the highest antioxidant activities. S. santolinifolia, S. eremophila and S. palestina, which have not been studied before, were the most active plants. These were more active than the previously studied species from this family, such as S. multicaulis and Marrubium vulgare. S. hydrangea and Gontscharovia popovii also showed high antioxidant activities. FRAP and DPPH assay results showed good correlations with the total phenolic contents of the plants, measured by the Folin-Ciocalteau assay (r2 = 0.925 and 0.799, respectively, p < 0.0001). In conclusion, our study shows that some Lamiaceae plants growing in Iran represent good potential sources of natural antioxidants useful for either prevention or treatment of oxidative stress-related diseases.

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Polyphenols of Rosa L. Leaves Extracts and their Radical Scavenging Activity

Zeitschrift für Naturforschung C ◽

10.1515/znc-2007-1-206 ◽

2007 ◽

Vol 62 (1-2) ◽

pp. 32-38 ◽

Cited By ~ 36

Author(s):

Renata Nowak ◽

Urszula Gawlik-Dziki

Keyword(s):

Antioxidant Activity ◽

Ellagic Acid ◽

Total Phenolic Content ◽

Phenolic Content ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Dry Weight ◽

Natural Antioxidants ◽

Total Phenolic

Antioxidant potential of Rosa L. leaves methanolic extracts was evaluated in vitro using a spectrophotometric method based on measuring the radical scavenging effect on 2,2-diphenyl- 1-picrylhydrazyl (DPPH) radicals. The contents of ellagic acid, quercetin and kaempferol in the extracts from leaves of seventeen rose species were determined using SPE-RPHPLC methods. Additionally, total phenolic content was determined spectrophotometrically according to the Folin-Ciocalteu procedure and calculated as gallic acid equivalents (GAE). Remarkable high antioxidant activity and high total phenolic content (5.7% < GAE < 15.2%), large ellagic acid (EA) content from 9.37 to 19.42 mg/g of dry weight, a quercetin content ranging from 3.68 to 15.81 mg/g of dry weight and kaempferol content from 1.25 to 9.41mg/g of dry weight were found in rose leaves. Significant correlation between EA (r2 = 0.6131), quercetin (r2= 0.5158), total phenolic content (r2= 0.8485) and antioxidant activity was observed. Basing on the studies conducted one may assume that the extracts of rose leaves are a rich source of natural antioxidants and could be used to prevent free-radical-induced deleterious effects.

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Chemical composition and antioxidant activity of three species of Cornukaempferia in Thailand

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d220952 ◽

2021 ◽

Vol 22 (9) ◽

Author(s):

THEERAPHAN CHUMROENPHAT ◽

Surapon Saensouk ◽

PIYAPORN SAENSOUK

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Antioxidant Activities ◽

Dry Weight ◽

Total Phenolic ◽

Antioxidant Power ◽

Plant Parts ◽

Ferric Reducing Antioxidant Power ◽

Dpph Scavenging Activity ◽

Dpph Scavenging

Abstract. Chumroenphat T, Saensouk S, Saensouk P. 2021. Chemical composition and antioxidant activity of three species of Cornukaempferia in Thailand. Biodiversitas 22: 4036-4044. Genus Cornukaempferia (Zingiberaceae) were used as medicinal plants in Thailand. Cornukaempferia has never been reported the chemical compounds and antioxidant activity before. This study was designed to identify the chemical composition and antioxidant activities in root, rhizome, stem, and leaves of Cornukaempferia aurantiflora, C. larsenii, and C. longipetiolata. DPPH scavenging activity and Ferric reducing antioxidant power (FRAP) assays assessed the chemical composition and antioxidant activity. The results showed that total phenolic content (TPC) and total flavonoid content (TFC) found different plant parts obtained in this study were in the order of root>rhizomes>leaves>?stems. The C. aurantiflora had a high TPC of 416.39 mg GAE/g dry weight, while individual phenolics, such as vanillic acid at 256.90 mg/g dry weight, showed high contents in the leaf part. For C. larsenii, TFC and individual flavonoids, such as catechin, have high 62.52 and 56.47 mg/100g dry weight values in the roots, respectively. The antioxidant activities in different species of different plant parts (DPPH: root>leaves?> rhizomes?>?stems; FRAP: leaves?>root>?rhizomes?>?stems). The genus Cornukaempferia is a rich source of bioactive compounds and antioxidant activity. Therefore, the genus Cornukaempferia will be used as medicine and food to produce products with health benefits in the future.

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Polyphenols Content and Antimicrobial, Antioxidant and Hemolytic Activities of Essential Oils from Four Selected Medicinal Plants Growing in Algeria

Biology Medicine & Natural Product Chemistry ◽

10.14421/biomedich.2020.92.65-75 ◽

2020 ◽

Vol 9 (2) ◽

pp. 65-75

Author(s):

Noureddine Halla ◽

Kebir Boucherit ◽

Bankaddour Zeragui ◽

Abdelkader Djelti ◽

Ziane Belkhedim ◽

...

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Essential Oils ◽

Radical Scavenging ◽

Reducing Power ◽

Extreme Environment ◽

Positive Control ◽

Mentha Piperita ◽

Antioxidant Power ◽

Dpph Method

The Saharan and steppe spontaneous plants are very characteristic because of their particular adaptation to the desert and extreme environment. Some species have pharmacological properties that give them a medicinal interest. The aim of the present work was to determine the polyphenol contents of essential oils obtained from four endemic plants growing in Algeria (Pituranthos scoparius, Myrtus nivellei, Rosmarinus officinalis and Mentha piperita), and study its biological activity, including antimicrobial, antioxidant, and hemolytic. The antimicrobial activity was evaluated by the microdilution method against twelve strains. The antioxidant activity was carried out by two methods (DPPH radical scavenging and reducing power). However, the hemolytic effect has been evaluated against the red blood cells. P. scoparius and M. piperita showed yields of essential oils higher than 1%. All the strains showed sensitivity against the essential oils tested with the exception of the C. albicans treated by R. officinalis essential oils. The most sensitive strain was C. albicans treated by P. scoparius essential oils by MIC of 0.0781 mg/mL, it was the same plant that shows the highest polyphenol content (14.78 ± 0.72 g GAE/g DS). The antioxidant activity by the DPPH method was greater for all essential oils tested by IC50 ranging from 0.69 ± 0.07 (R. officinalis) to 30.67 ± 2.12 mg/mL (M. nivellei). The R. officinalis essential oils reported more antioxidant power than the positive control (ascorbic acid). In reducing iron, it was the R. officinalis essential oils which were found to be the most active with an EC50 concentration of 9.67 ± 1.36 mg/mL. After 120 min incubation, minimal haemolysis (10%) was obtained with essential oils of R. officinalis at a concentration of 0.39 mg/mL. We conclude that P. scoparius essential oils showed the high content of polyphenols and R. officinalis essential oils reported more antioxidant power than the positive control (ascorbic acid).

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Antioxidant Activity and Total Flavonoid of Carica papaya L. Leaves with Different Varieties, Maturity and Solvent

Jurnal Agritech ◽

10.22146/agritech.12813 ◽

2019 ◽

Vol 39 (1) ◽

pp. 54 ◽

Cited By ~ 1

Author(s):

Fatma Zuhrotun Nisa ◽

Mary Astuti ◽

Sofia Mubarika Haryana ◽

Agnes Murdiati

Keyword(s):

Antioxidant Activity ◽

Carica Papaya ◽

Radical Scavenging ◽

Total Flavonoid ◽

Water Extraction ◽

Antioxidant Power ◽

Mature Leaves ◽

Dpph Method ◽

Young Leaves ◽

Ferric Reducing Antioxidant Power

Carica papaya leaves are one of the vegetables consumed by Indonesian people, especially in Java Island. Carica papaya is easy to grow in Indonesia and has many variants, so, Carica Papaya leaves is a local potent to be developed for functional food and nutraceutical. The aim of this study was to investigate antioxidant activity and total flavonoids of Carica papaya leaves with different varieties, maturity and solvent. Carica papaya leaves (CPL) was firstly extracted by methanol to select two CPLs with high antioxidant capacity and total flavonoid. The two selected CPLs were further tested with different ages mainly young and mature leaves. One selected CPL was further tested with different extraction solvents. Antioxidant activity was determined by 2.2 diphenyl-1-picrylhydrazyl, DPPH and Ferric reducing antioxidant power, FRAP. This study used five varieties of Carica papaya leaves, namely Bangkok, California, Purple, Golden and Grendel. The result showed that Golden and Grendel varieties had a higher percentage of radical scavenging property than the others, which was 78.37% and 77.40% by the DPPH method. Grendel and Purple had a higher percentage of radical scavenging property, which was 45.82 and 34.32 mmol/mg. Grendel and Purple had a higher total flavonoid property, which was 50.33 and 46.02 µg/g. Mature leaves had a higher percentage of radical scavenging property than young leaves by DPPH and FRAP methods. Mature leaves had a higher total flavonoid property than young leaves in both Grendel and Purple. Grendel had a higher antioxidant activity and a higher total flavonoid property than Purple. Grendel with water extraction had a higher antioxidant activity by DPPH and FRAP methods. The total flavonoid of Grendel papaya leaves’ extract with water extraction was lower than ethanol 70% and methanol.

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Influence of Packaging Material on Polyphenol Content and Antioxidant Activity in Some Commercial Beers

10.26434/chemrxiv.12245549 ◽

2020 ◽

Author(s):

Mihaela Mirela Bratu ◽

Semaghiul Birghila ◽

Antoanela Popescu ◽

Bogdan Stefan Negreanu- Pirjol ◽

Marius Radu

Keyword(s):

Antioxidant Activity ◽

Plastic Material ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Packaging Material ◽

Polyphenol Content ◽

Antioxidant Power ◽

Phenolic Contents ◽

Dpph Method ◽

Ferric Reducing Antioxidant Power

<div><div><div><div><p>Using two methods (ferric reducing antioxidant power and radical scavenging activity), the total polyphenol content (Folin–Ciocalteu reagent) and polyphenol patterns (HPLC) in 10 commercial lager beer brands produced in Romania was determined. Samples bottled in glass, plastic and aluminium packages were analysed for each brand when available. Results have indicated considerable variations in the total and individual phenolic contents as well as antioxidant activity across beer brands. A statistical analysis was performed to assess the influence of packaging type on the antioxidant activity and phenolic content of the beers. Statistical differences were found between the DPPH and FRAP methods in glass, aluminium and plastic material. Moreover, the antioxidant activity based on the DPPH method is influenced by the type of packaging material, while in the case of FRAP method, no statistical difference was reported. Furthermore, the same analysis has shown that the polyphenol concentration is invariant to the type of material.</p></div></div></div></div>

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CHEMO-PROFILING AND ASSESSMENT OF IN VITRO ANTIOXIDANT EFFICACY OF EIGHT FERNS OF DARJEELING HIMALAYAS, INDIA

10.36106/ijsr/0744528 ◽

2021 ◽

pp. 25-27

Author(s):

Nishika Jaishee ◽

Rohini Lama ◽

Usha Chakraborty

Keyword(s):

Antioxidant Activity ◽

High Performance ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Dry Weight ◽

Natural Antioxidants ◽

Phenolic Contents ◽

Darjeeling Himalayas

AIM: The present study was performed to prole some phenolics and explore the antioxidant effect of eight locally available ferns collected from different places of Darjeeling Himalayas, India. Methods: The antioxidant activities of methanol (MeOH) extract was evaluated by DPPH free radical scavenging activity. Qualitative analysis of phenol was done using standard methods. Further, characterization of phenolics was done using High performance liquid chromatography. Result: The content of phenolics ranged from 6.77 to 60.066mg FAE/g dry weight. The DPPH antioxidant activity expressed as IC values 50 revealed Nephrolepis cordifolia and Microsorum punctatum to exhibit highest and lowest antioxidative activity respectively. Moderate correlation 2 (R =0.547) was observed between the total phenolics content and antioxidant activity. HPLC analysis of phenolics from all the investigated plants revealed the presence of caffeic acid, ferulic acid and salicylic acid while the other phenolics such as phloroglucinol, gallic acid, pyrogallol, 3,4- dihy droxybenzoic acid, catechol, catechin, chlorogenic acid, caffeine, vanillic acid and cinnamic acid were not uniformly present in all the plants. The phenolic contents values showed wide variation among themselves, as well as within different plants. These ferns with considerable amount of phenolics can be the potential source of natural antioxidants.

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