scholarly journals Effect of humate on the activity of bacterial fertilizers

2021 ◽  
Vol 31 (3) ◽  
pp. 127-131
Author(s):  
Enkhmaa Erdenetugs ◽  
Sunjidmaa Otgonbayar
Keyword(s):  
Cell Growth ◽  
Laboratory Condition ◽  
Bacterial Growth ◽  
Bacterial Cell ◽  
Growth And Development ◽  
Nutrient Broth ◽  
Beneficial Bacteria

The plots of experiment was control (two different efficient bacteria), humate fertilizer (250 ml) in laboratory condition. The use of bacteria and humate fertilizer showed that the beneficial bacterial cell growth and development of each version increased from control, and some of the version were highly activaties. In result, beneficial bacteria have been found to be resistant to humate fertilizer and the humic fertilizer promotes bacterial growth. Бактерийн бордооны идэвхид гуматын нөлөөг судалсан дүн Дархан-Ризо шингэн бордоон дахь ашигтай бактерийн бүрэлдэхүүнд гуматын нөлөөг илрүүлэх зорилгоор лабораторийн туршилтыг гүйцэтгэв. Судалгааны материал болгон УГТХ-ийн Хөрсний микробиологийн лабораторийн өсгөврийн санд хадгалагдаж байгаа ризобактерийн эх өсгөвөр дээр гуматын бордоог 250 мл/тн тунгаар нэмж Nutrient broth шингэн тэжээлийн орчинд ургуулж хяналттай харьцуулан спектрофотометр багажаар эсийн нягтыг тодорхойлов. Судалгааны дүнгээр ризобактерийг гуматтай хольж тэжээлийн орчинд ургууулахад эхний 48 цагийн өсөлт хөгжил гумат холиогүй хувилбартай ижил зүй тогтолоор өсч байв. Харин 72 цагийн дараа гумат холиогүй хувилбарын эсийн нягт 2.422 байсан бол гуматтай орчинд агуулагдаж байгаа бактерийн эс 4.029 нягтралтай байв. Энэ нь судалгаанд ашиглаж буй гумат ризобактерийн идэвхийг дарангуйлах үйлчилгээгүй, харин өсөлтийн эрчмийг хяналтаас 1.6 дахин нэмэгдүүлдэг болохыг тогтоов. Цаашид талбайн нөхцөлд таримлуудыг бордох технологитой хамтатган нарийвчилсан судалгааны ажлуудыг хийж гүйцэтгэх нь зүйтэй юм.  Түлхүүр үг: Humate fertilizer, rhizobium biofertilizer, beneficial bacteria, spectrophotometr

scholarly journals Bacterial Cellulose as a Substrate for Microbial Cell Culture

2014 ◽  
Vol 80 (6) ◽  
pp. 1926-1932 ◽  
Author(s):  
Na Yin ◽  
Thiago M. A. Santos ◽  
George K. Auer ◽  
John A. Crooks ◽  
Piercen M. Oliver ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Growth ◽  
Physicochemical Properties ◽  
Stationary Phase ◽  
Bacterial Cellulose ◽  
Growth Rates ◽  
Bacterial Cell ◽  
Acetobacter Xylinum ◽  
Primary Factor ◽  
Nutrient Diffusion

ABSTRACTBacterial cellulose (BC) has a range of structural and physicochemical properties that make it a particularly useful material for the culture of bacteria. We studied the growth of 14 genera of bacteria on BC substrates produced byAcetobacter xylinumand compared the results to growth on the commercially available biopolymers agar, gellan, and xanthan. We demonstrate that BC produces rates of bacterial cell growth that typically exceed those on the commercial biopolymers and yields cultures with higher titers of cells at stationary phase. The morphology of the cells did not change during growth on BC. The rates of nutrient diffusion in BC being higher than those in other biopolymers is likely a primary factor that leads to higher growth rates. Collectively, our results suggest that the use of BC may open new avenues in microbiology by facilitating bacterial cell culture and isolation.

scholarly journals The Ethanologenic Bacterium Zymomonas mobilis Divides Asymmetrically and Exhibits Heterogeneity in DNA Content

2021 ◽  
Vol 87 (6) ◽  
Author(s):  
Katsuya Fuchino ◽  
Helena Chan ◽  
Ling Chin Hwang ◽  
Per Bruheim
Keyword(s):  
Cell Growth ◽  
Single Cell ◽  
Cell Size ◽  
Dna Content ◽  
Bacterial Cell ◽  
Zymomonas Mobilis ◽  
Biofuel Production ◽  
Public Attention ◽  
Content Type ◽  
Cell Organization

ABSTRACT The alphaproteobacterium Zymomonas mobilis exhibits extreme ethanologenic physiology, making this species a promising biofuel producer. Numerous studies have investigated its biology relevant to industrial applications and mostly at the population level. However, the organization of single cells in this industrially important polyploid species has been largely uncharacterized. In the present study, we characterized basic cellular behavior of Z. mobilis strain Zm6 under anaerobic conditions at the single-cell level. We observed that growing Z. mobilis cells often divided at a nonmidcell position, which contributed to variant cell size at birth. However, the cell size variance was regulated by a modulation of cell cycle span, mediated by a correlation of bacterial tubulin homologue FtsZ ring accumulation with cell growth. The Z. mobilis culture also exhibited heterogeneous cellular DNA content among individual cells, which might have been caused by asynchronous replication of chromosome that was not coordinated with cell growth. Furthermore, slightly angled divisions might have resulted in temporary curvatures of attached Z. mobilis cells. Overall, the present study uncovers a novel bacterial cell organization in Z. mobilis. IMPORTANCE With increasing environmental concerns about the use of fossil fuels, development of a sustainable biofuel production platform has been attracting significant public attention. Ethanologenic Z. mobilis species are endowed with an efficient ethanol fermentation capacity that surpasses, in several respects, that of baker’s yeast (Saccharomyces cerevisiae), the most-used microorganism for ethanol production. For development of a Z. mobilis culture-based biorefinery, an investigation of its uncharacterized cell biology is important, because bacterial cellular organization and metabolism are closely associated with each other in a single cell compartment. In addition, the current work demonstrates that the polyploid bacterium Z. mobilis exhibits a distinctive mode of bacterial cell organization, likely reflecting its unique metabolism that does not prioritize incorporation of nutrients for cell growth. Thus, another significant result of this work is to advance our general understanding in the diversity of bacterial cell architecture.

Activated Akt promotes increased resting T cell size, CD28-independent T cell growth, and development of autoimmunity and lymphoma

2003 ◽  
Vol 33 (8) ◽  
pp. 2223-2232 ◽  
Author(s):  
Jeffrey C. Rathmell ◽  
Rebecca L. Elstrom ◽  
Ryan M. Cinalli ◽  
Craig B. Thompson
Keyword(s):  
T Cell ◽  
Cell Growth ◽  
Cell Size ◽  
Growth And Development ◽  
T Cell Growth

scholarly journals Immobilization of Aeromonas bivalvium PT2 Cells with Alginate and Measurement of Chitinolytic Activities

2020 ◽  
Vol 147 ◽  
pp. 03020
Author(s):  
Dita P. Saputri ◽  
Ustadi
Keyword(s):  
Bacterial Cell ◽  
Cell Immobilization ◽  
Chitinase Activity ◽  
Nutrient Broth ◽  
Chitinolytic Activity ◽  
Immobilized Bacteria ◽  
Chitinolytic Bacteria ◽  
Bacterial Immobilization ◽  
The Stability ◽  
Bacterial Cell Immobilization

Aeromonas bivalvium is one of the chitinolytic bacteria that able to degrade chitin into its derivatives. These bacteria can only be used once during the fermentation process, which is less profitable to be applied in industrial scale. This limitation can be solved by bacterial immobilization method. This study aimed to determine the effect of bacterial cell immobilization on chitinolytic activity and to determine the stability of the immobilized bacteria during repeated usage. Bacterial cell immobilization was carried out by entrapment method with 1% sodium alginate matrix. Immobilized bacteria was cultured in two different mediums, namely nutrient broth (NB) and nutrient broth (NB) added with colloidal chitin (NB + K). Tests for chitinolytic activity were carried out in bacteria. In addition, the stability of immobilized bacteria was also tested for chitinolytic activity with repeated removal and use. The result shows that the effectiveness of immobilization on average is 91.8%. Immobilization did not significantly affect chitinolytic activity when compared with bacteria without immobilization. Immobilized bacteria in this study has similar performance as bacteria without immobilization. The results of the stability tests including chitinase activity and NAG released indicated a significant decline during repeated usage with maximum usage of three times.

GENERATIVE CYCLES OF PROTEIN AND TRANSAMINASE IN THE GROWING CORN RADICLE

1959 ◽  
Vol 37 (4) ◽  
pp. 621-639 ◽  
Author(s):  
F. S. Cook
Keyword(s):  
Enzyme Activity ◽  
Cell Growth ◽  
Growth And Development ◽  
Specific Protein ◽  
Soluble Nitrogen ◽  
Transaminase Activity ◽  
Cell Numbers ◽  
Nitrogen Levels ◽  
Linear Distance ◽  
Cellular Development

Measurements have been made of growth rates, cell numbers, fresh and dry weights, protein and soluble nitrogen levels, and glutamic–aspartic transaminase activity in six successive 2 mm segments of the radicles of 3-day-old corn seedlings. The measured quantities of protein and enzyme activity are related to the stage of average cellular development, to a linear distance scale along the axis of the radicle, and to the time scale. Increments per cell per hour during cell growth are therefore computed. An attempt is made to explain the significance of the genesis of the transaminase in the growth and development of the radicle cells, to the concurrent genesis of total and specific protein, and to other generative cycles.

Characterization of Polysaccharide Production of Haemophilus influenzae Type b and Its Relationship to Bacterial Cell Growth

2003 ◽  
Vol 110 (2) ◽  
pp. 91-100 ◽  
Author(s):  
Mickie Takagi ◽  
Joaquin Cabrera-Crespo ◽  
Júlia Baruque-Ramos ◽  
Teresa Cristina Zangirolami ◽  
Isaias Raw ◽  
...  
Keyword(s):  
Cell Growth ◽  
Haemophilus Influenzae ◽  
Bacterial Cell ◽  
Haemophilus Influenzae Type ◽  
Haemophilus Influenzae Type B ◽  
Type B ◽  
Polysaccharide Production
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