scholarly journals The Possibility of obtaining inulin from the tubers of Helianthus tuberosus L and Inula helenium L

2021 ◽  
Vol 31 (3) ◽  
pp. 174-179
Author(s):  
Erdenechimeg Namjil ◽  
Oyundari Ganzorig ◽  
Orgilmaa Bayarsaikhan ◽  
Munkhtsetseg Byambaa ◽  
Bayarmaa Barkhuu
Keyword(s):  
Extraction Methods ◽  
Helianthus Tuberosus ◽  
Microwave Extraction ◽  
Glucose Residue ◽  
Light Yellow ◽  
Natural Polysaccharide ◽  
Inula Helenium ◽  
Ft Ir ◽  
Ultrasound Assisted ◽  
Conventional Extraction

Inulin is a natural polysaccharide abundant in plants. In the latest decades, in Mongolia, have been cultivated some plants which used in traditional medicine. Inulin polysaccharides were isolated from the roots of the Helianthus tuberosus L. and Inula helenium L. by ultrasound-assisted, microwave extraction, and conventional extraction methods. This polysaccharide is light yellow, tasteless, powder. The polysaccharide structure was confirmed by infrared spectroscopy (FT-IR). The poly fructose content of the Helianthus tuberosus L. ranged from 69 to 84%; in comparison, Inula helenium L ranged from 13 to 51%.  The IR-FT spectra revealed typical inulin structure - 820, 876, and 937 cm-1 with terminated α-D-glucose residue. Булцуут цэцэг (Helianthus tuberosus l.) Өндөр зоосон цэцэг (Inula helenium l.) -ийн үндэснээс инулин гарган авах боломж Инулин нь ургамалд элбэг байдаг байгалийн полисахарид бөгөөд манай оронд инулинаар баялаг зарим төрлийн ургамлыг амжилттай нутагшуулан тарималжуулж байгаа билээ. Манай оронд нутагшсан Булцуут цэцэг (Helianthus tuberosus L.) болон Өндөр зоосон цэцэг (Inula helenium L.)  ургамлын үндэснээс инулин полисахаридыг хэт авиа (ХА), богино долгион (БД) болон уламжлалт хандлах аргын тусламжтайгаар ялган авлаа. Энэ полисахарид нь ямар нэгэн амтгүй, цайвар шаргал өнгөтэй, нунтаг хэлбэртэй. Уг полисахаридын бүтцийг нил улаан туяаны спектроскопийн (FT-IR) аргаар баталгаажуулсан ба инулины агууламжийг нийт фруктозод шилжүүлэн тооцов. Булцуут цэцгийн полифруктаны агууламж  69-84%, Өндөр зоосон цэцгийнх 13-51% хооронд хэлбэлзэж байсан бөгөөд богино долгионы тусламжтай ялган авсан инулины полифруктаны агууламж хамгийн өндөр байв. НУТ-ны спектрийн шингээлтээр инулины бүтцийн гол зурвасууд нь 820, 876 саяны хэсэгт болон фураноз хэлбэртэй b-D-фруктоз, харин 937 см-1 саяны хэсэгт пираноз хэлбэртэй a-D-глюкозын үлдэгдэл байгааг харуулж байна.  Түлхүүр үг: булцуут цэцэг, инулин, НУТ спектроскоп, өндөр зоосон цэцэг, полифруктан

scholarly journals Effects of Ultrasound-Assisted Extraction and Solvent on the Phenolic Profile, Bacterial Growth, and Anti-Inflammatory/Antioxidant Activities of Mediterranean Olive and Fig Leaves Extracts

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1718 ◽  
Author(s):  
Cristina Alcántara ◽  
Tihana Žugčić ◽  
Radhia Abdelkebir ◽  
Jose V. García-Pérez ◽  
Anet Režek Jambrak ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Bacterial Growth ◽  
Extraction Methods ◽  
Olive Leaves ◽  
Ultrasound Assisted Extraction ◽  
Phenolic Profile ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Conventional Extraction ◽  
Anti Inflammatory

Mediterranean plants, such as fig and olive leaves, are well-known to exert beneficial effects in humans because of the presence of a wide range of bioactive compounds. However, scarce information regarding the impact of extraction methods, such as ultrasound and types of solvents, on their profile of antioxidant and anti-inflammatory compounds is provided. In addition, no information is available on the effects of extraction methods and solvents on the inhibition of pathogenic bacteria or promoting probiotic growth. In this scenario, this study was aimed to study the effects of ultrasound-assisted extraction (UAE) and solvent on the phenolic profile (Triple TOF-LC-MS/MS), antioxidant and anti-inflammatory compounds of olive and fig leaves. Results showed that UAE extracted more carotenoids compared to conventional extraction, while the conventional extraction impacted on higher flavonoids (olive leaves) and total phenolics (fig leaves). The antioxidant capacity of aqueous extract of fig leaves was three times higher than the extract obtained with ethanol for conventional extraction and four times higher for UAE. In general terms, hydroethanolic extracts presented the highest bacterial growth inhibition, and showed the highest anti-inflammatory activity. In conclusion, these side streams can be used as sources of bioactive compounds for further development of high-added-value products.

Comparison of Conventional and Ultrasound-Assisted Extraction Techniques for Extraction of Phenolic Compounds from Coconut Husk

2019 ◽  
Vol 891 ◽  
pp. 83-89
Author(s):  
Attapon Nitiwattananon ◽  
Saipin Thanachasai
Keyword(s):  
Solvent Extraction ◽  
Phenolic Compounds ◽  
Extraction Methods ◽  
Extraction Yield ◽  
Agitation Speed ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Conventional Solvent Extraction ◽  
Ultrasound Assisted ◽  
Conventional Extraction

In this study, ultrasound-assisted extraction (UAE) was compared with conventional extraction methods, including conventional solvent extraction without agitation (CSE), conventional solvent extraction with agitation at 50 rpm (CSE50) and 150 rpm (CSE150), for the extraction of phenolic compounds from coconut (Cocos nucifera L.) husk. The extraction yield, total phenolic content (TPC) and total flavonoid content (TFC) were examined. The antioxidant capacity of C. nucifera extracts was determined by using 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2’-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. Experimental results showed that UAE gave the highest extraction yield, TPC, TFC and antioxidant capacities (ABTS and DPPH), followed by CSE150, CSE50 and CSE, respectively. UAE was found to be more effective than conventional extraction methods. Conventional solvent extraction with higher agitation speed exhibited higher extraction efficiency than those with lower agitation speed and without agitation.

scholarly journals Downstream Processing Stages, Comparative Analysis & Extraction Methodologies in Generation of Cannabinoids from Cannabis Sativa L. (Hemp)

2021 ◽  
Author(s):  
Lakshya Sharma
Keyword(s):  
Supercritical Fluid ◽  
Cannabis Sativa ◽  
Downstream Processing ◽  
Extraction Methods ◽  
Ultrasound Assisted Extraction ◽  
Extraction Technology ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Conventional Extraction

Due to the intoxicating effects of only a few cannabinoids, the cannabis plant has long been prohibited by legislation in several countries. Recent scientific advancements, along with a growing public awareness of cannabis as a medicinal commodity, prompted law reform, resulting in a historic shift in which demand increased tenfold in less than five years. The technology necessary for cannabis processing and extraction of the most valuable chemical components from the cannabis flower, on the other hand, remains the processing bottleneck. The downstream processing stages and concepts involved in generating cannabinoids from Cannabis Sativa L. (Hemp) biomass are discussed in this study. I evaluated and criticised several pre-treatment procedures and technical alternatives available for large-scale extraction in both categories by dividing extraction technology into seed and trichome. The major focus was on solvent extraction methods, as well as the important decision-making criteria at each stage and the applicable contemporary technology in the sector. I looked at the variables that impact cannabis transformation and how they affect the medicinal functioning of the finished goods. According to current trends, extraction technologies are constantly reviewed and improved, yet they still fall short of market demands. Cannabis sativa has hundreds of bioactive chemicals, making it one of the oldest therapeutic plants utilised by humans. Although the plant's medicinal benefits are undeniable, the biological consequences and interaction of these chemicals are yet unknown. These chemicals' extraction techniques are becoming an important element of current Cannabis-based therapy. Despite this, little is known about how different techniques impact the final composition of Cannabis extracts and, as a result, their medicinal benefits. Different extraction methods, such as maceration, Soxhlet, ultrasound-assisted extraction, and supercritical CO2 extraction methods, were evaluated in this study. The extracts were tested in vitro on human colon cancer and healthy colon cells for cannabis content, antioxidant effects, and in vitro bioactivity. Findings indicate that properly produced cannabis extracts can dramatically reduce cancer cell viability while sparing healthy cells from harmful effects. However, because post-processing of extracts alters not only the actual quantities of the various cannabinoids, but also their relative ratio to the main extracts, it is difficult to anticipate therapeutic response solely on the composition of the crude extract. These effects must be carefully addressed while developing novel medicinal extracts in the future. The natural non-psychoactive and psychoactive cannabinoids in cannabis are increasing its medicinal relevance. To effectively use the natural cannabinoids for therapeutic and forensic reasons, efficient extraction and quantification are required. In comparison to most conventional extraction methods, the supercritical fluid extraction (SFE) process has gained increasing interest due to its selective extraction, short processing time (partly due to the efficient solvent removal process – supercritical fluid to vapour – leaving a solvent free product), low operating cost, and low environmental impact. Microwave-assisted extraction, solid phase microextraction, hard-cap espresso, Soxhlet extraction, high-throughput homogenization, ultrasound-assisted extraction, vacuum distillation of lipid-based extract, and liquid–liquid extraction are discussed as advantages of SFE of cannabinoids over conventional extraction procedures.

scholarly journals Agro-industrial waste as a source of bioactive compounds: ultrasound-assisted extraction from blueberry (Vaccinium myrtillus) and raspberry (Rubus idaeus) pomace

2021 ◽  
Vol 43 ◽  
pp. e55564
Author(s):  
Suelen Siqueira dos Santos ◽  
Carolina Moser Paraíso ◽  
Letícia Misturini Rodrigues ◽  
Grasiele Scaramal Madrona
Keyword(s):  
Bioactive Compounds ◽  
Energy Efficient ◽  
Extraction Method ◽  
Rubus Idaeus ◽  
Total Phenolic ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Conventional Extraction ◽  
Gallic Acid Equivalent

Blueberry and raspberry pomace are a rich source of bioactive compounds that have not been commercially utilized yet, and ultrasound-assisted technology can efficiently extract these compounds. Also, the use of water as a solvent added to the ultrasound-assisted technology improves this eco-friendly process. Therefore, an aqueous eco-friendly extraction, including extraction time and ultrasound presence or absence (conventional extraction) was performed in order to extract bioactive compounds from blueberry and raspberry pomace. Response parameters included levels of anthocyanins, phenolic compounds, and flavonoids, and antioxidant activity determined by DPPH, ABTS, and FRAP methods. Analysis of variance results indicated that ultrasound-assisted extraction for 45 min. was feasible to extract the bioactive compounds. The antioxidant content of the extract obtained by the ultrasound-assisted process was 1.4 times higher on average and the total phenolic concentration was 1.6 times higher (for blueberry 5.02 and for raspberry 2.53 mg gallic acid equivalent/g) compared with those obtained by the conventional process. Thus, the ultrasound-assisted extraction method can be a profitable alternative to extract bioactive compounds from blueberry and raspberry pomace, as it is energy efficient, requires fewer chemicals, and produces less effluent. This eco-friendly technology is therefore viable for food, nutraceutical, and cosmetic industries, and also for reducing food waste.

scholarly journals Synthesis and Heme Polymerization Inhibitory Activity (HPIA) Assay of Antiplasmodium of (1)-N-(3,4-Dimethoxybenzyl)-1,10-Phenanthrolinium Bromide from Vanillin

2014 ◽  
Vol 14 (1) ◽  
pp. 1-6
Author(s):  
Dhina Fitriastuti ◽  
Muhammad Idham Darussalam Mardjan ◽  
Jumina Jumina ◽  
Mustofa Mustofa
Keyword(s):  
Melting Point ◽  
Sodium Borohydride ◽  
Inhibitory Activity ◽  
Veratryl Alcohol ◽  
Activity Assay ◽  
Light Yellow ◽  
Grinding Method ◽  
Methylation Product ◽  
Ft Ir ◽  
Phenanthroline Monohydrate

The synthesis of (1)-N-(3,4-dimethoxy-benzyl)-1,10-phenanthrolinium bromide had been conducted from vanillin. Heme polymerization inhibitory activity assay of the synthesized antiplasmodium has also been carried out. The first step of reaction was methylation of vanillin using dimethylsulfate and NaOH. The mixture was refluxed for 2 h to yield veratraldehyde in the form of light yellow solid (79% yield). Methylation product was reduced using sodium borohydride (NaBH4) with grinding method and yielded veratryl alcohol in the form of yellow liquid (98% yield). Veratryl alcohol was brominated using PBr3 to yield yellowish black liquid (85% yield). The final step was benzylation of 1,10-phenanthroline monohydrate with the synthesized veratryl bromide under reflux condition in acetone for 14 h to afford (1)-N-(3,4-dimethoxy-benzyl)-1,10-phenanthrolinium bromide (84%) as yellow solid with melting point of 166-177 °C. The structures of products were characterized by FT-IR, GC-MS and 1H-NMR spectrometers. The results of heme polymerization inhibitory activity assay of (1)-N-(3,4-dimethoxybenzyl)-1,10-phenanthrolinium bromide showed that it had IC50 HPIA of 3.63 mM, while chloroquine had IC50 of4.37 mM. These results indicated that (1)-N-(3,4-dimethoxybenzyl)-1,10-phenanthrolinium bromide was more potential antiplasmodium than chloroquine.

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