Antifungal Activity Screening and HPLC Analysis of Crude Extract from Tectona grandis, Shilajit, Valeriana wallachi

10.5580/2390 ◽  
2008 ◽  
Vol 5 (2) ◽  
Keyword(s):  
Antifungal Activity ◽  
Crude Extract ◽  
Hplc Analysis ◽  
Tectona Grandis ◽  
Activity Screening

Homogenate Extraction of Dihydroartemisinin from Artemisia Hedinii and Its Antifungal Activity

2021 ◽  
Author(s):  
Cong You ◽  
Jun Yu ◽  
Guangjiong Qin ◽  
JinPeng Yang ◽  
Chunlei Yang ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Crude Extract ◽  
Spore Germination ◽  
Alternaria Alternata ◽  
Low Cost ◽  
Pathogenic Fungi ◽  
Extraction Yield ◽  
Optimal Conditions ◽  
Extraction Technology ◽  
Homogenate Extraction

Abstract Background Artemisia hedinii is a well-known traditional Chinese medicine. It can be used to extract dihydroartemisinin (DHA). Objective The purpose of this study was to explore the optimal conditions for the homogenate extraction of DHA from A. hedinii and the antifungal activity of DHA. Methods In this study, single factor experiments and response surface method were used to determine the optimal extraction conditions of crude extract and DHA, the method of spore germination was used to study the antifungal activity of DHA to Alternaria alternata. Result The optimal conditions were found as fellow: ratio of liquid to material 22 mL/g; Extraction time 60 s; soaking time 34 min. Under these conditions, extraction yield of DHA was (1.76 ± 0.04%). When the concentration of crude extract were 0.5 and 8 mg/mL, the spore germination inhibition rates of Alternaria alternata were (17.00 ± 2.05%) and (92.56 ± 2.01%), which were 3.34 and 1.15 times that of DHA standard, respectively. Conclusion Homogenate extraction technology is a fast and efficient method to extract DHA from A. hedinii. The crude extract has significant antifungal activity against A. alternata with low cost, which provides a possibility for the use of DHA in the prevention and treatment of plant pathogenic fungi. Highlights The optimum conditions of the extraction of DHA from A. hedinii by homogenate extraction were obtained. DHA has antifungal activity against A. alternata. Compared with pure DHA, the crude extract has stronger antifungal activity against A. alternata.

scholarly journals Antifungal Properties of Crude Extracts, Fractions, and Purified Compounds from Bark ofCuratella americanaL. (Dilleniaceae) againstCandidaSpecies

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Cleyton Eduardo Mendes de Toledo ◽  
Patrícia Regina Santos ◽  
João Carlos Palazzo de Mello ◽  
Benedito Prado Dias Filho ◽  
Celso Vataru Nakamura ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Virulence Factors ◽  
Crude Extract ◽  
Ethyl Acetate Fraction ◽  
Yeast Culture ◽  
Ethnomedicinal Plant ◽  
Fluconazole Resistant ◽  
Resistant Strains ◽  
Preliminary Study ◽  
Curatella Americana

The ethnomedicinal plantCuratella americanaL. (Dilleniaceae) is a common shrub in the Brazilian cerrado, in which crude extract showed antifungal activity in a preliminary study. In this work, the antifungal and cytotoxic properties of the crude extract, fractions, and isolated compounds fromC. americanawere evaluated against the standard yeast strainsCandida albicans,C. tropicalis, andC. parapsilosis, clinical isolates, and fluconazole-resistant strains. The combinatory effects between subfractions and isolated compounds and effects on cell morphology, virulence factors, and exogenous ergosterol were also evaluated. The MIC obtained against theCandidaspecies including fluconazole-resistant strain ranged from 15.3 to 31.3 µg/mL for crude extract, 3.9 to 15.6 µg/mL for ethyl acetate fraction, and 7.8 to 31.3 µg/mL for subfractions. The isolated compounds identified as 4′-O-methyl-catechin, epicatechin-3-O-gallate, and 4′-O-methyl-catechin-3-O-gallate showed lower antifungal activity than the crude extract and fractions (MIC ranging from 31.3 to 125.0 µg/mL). The addition of exogenous ergosterol to yeast culture did not interfere in the antifungal activity of the extract and its fractions. Synergistic antifungal activity was observed between subfractions and isolated compounds. The effects on virulence factors and the different mechanisms of action compared to fluconazole and nystatin suggest that this ethnomedicinal plant may be an effective alternative treatment for candidiasis.

GROWTH INHIBITORY ACTIVITIES OF THE RHIZOME CRUDE EXTRACT OF Curcuma longa ON THE HUMAN PATHOGENIC FUNGUS Mucor circinelloides

2020 ◽  
Vol 65 (10) ◽  
pp. 82-91
Author(s):  
Phuong Nguyen Anh ◽  
Mai Le Thi Tuyet ◽  
Trung Trieu Anh
Keyword(s):  
Antifungal Activity ◽  
Crude Extract ◽  
Fungal Infections ◽  
Early Stage ◽  
Curcuma Longa ◽  
Pathogenic Fungus ◽  
Mucor Circinelloides ◽  
Antifungal Drugs ◽  
Diffusion Method ◽  
Dilution Method

Mucormycosis is an uncommon but life-threatening invasive fungal infection, mostly occurs in immunocompromised patients. Lacking the appropriate antifungal drugs is one of the reasons that lead to difficulties in the management of mucormycosis. Curcuma longa has been used traditionally and widely to treat various diseases, including fungal infections. In the search for novel antifungal compounds from natural resources, we evaluated the effect of rhizome crude extract of C. longa on Mucor circinelloides – a causal agent of mucormycosis. The results of screening, using broth dilution method and agar-well diffusion method, showed that the C. longa extract exhibited promising antifungal activity against the fungus M. circinelloides. In liquid medium, C. longa extract decreased the ability of spore germination and the speed of hyphae formation of M. circinelloides decreased by up to approximately 70% and 90%, respectively. Besides, in a solid medium, the crude extract presented similar activity with amphotericin B (400 μg\mL) in decreasing the growth of M. circinelloides by nearly 77%. Moreover, the extract of C. longa also likely to induce the yeast-like type of growth of the dimorphic M. circinelloides in the early stage. These results suggest the plant could be a potential source for further study on biochemical components and the mechanism of its antifungal activity.

scholarly journals DAYA HAMBAT EKSTRAK KULIT JATI (Tectona grandis Linn F) TERHADAP PERTUMBUHAN JAMUR PELAPUK KAYU Schizophyllum commune Fries

2019 ◽  
Vol 7 (3) ◽  
Author(s):  
Ferry Vernando Hutabarat ◽  
Farah Diba ◽  
Lolyta Sisillia
Keyword(s):  
Antifungal Activity ◽  
Screening Test ◽  
Schizophyllum Commune ◽  
Wood Decay ◽  
Petri Dish ◽  
Tectona Grandis ◽  
Bark Extract ◽  
Phytochemical Screening ◽  
Decay Fungi ◽  
Wood Decay Fungi

The aim of the research was to examine the antifungal activity and the most effective concentration of teak bark (Tectona grandis Linn F) extract in inhibiting wood decay fungi Schizophyllum commune Fries. Teak bark was derived from BKPH Ledok, Sambong district, Blora Regency, West Java. The Teak bark made into particle with size pass of 40 mesh and retained 60 mesh. Then one kilogram of teak bark particle was maceration with 10 liter of aquadest. The extract then mixes with potatoes dextrose agar (PDA) with several concentration i.e. 0%, 0.25%, 0.5%, 0.75% and 1% and pour into petri dish. One isolate of Schizophyllum commune was put in the center of PDA in each petri dish then incubated for nine days. The bioactive compound of extract teak bark was evaluation used phytochemical screening. Result of the research showed that teak bark extract at concentration 0.25% and 0.5% has antifungal activity values was 27.98% and 40.38% and categorized as medium inhibition growth of S. commune. Meanwhile at concentration 0.75% and 1% the AFA values was 59.94% and 65.51% categorized as strong inhibition growth of S. commune. The bioactive compounds from phytochemical screening test were alkaloid, flavonoid, terpenoid, fenolik, antrakuinon, and saponin. The extract of teak bark with concentration 0.75% and 1% are the most effective concentrations in inhibiting the growth of wood decay fungi S. commune Fries.Keywords : antifungal, phytochemical screening test, Schizophyllum commune, Tectona grandis

scholarly journals Manganese (II) and Cobalt (II) Acetylacetonates as Antimicrobial Agents

2019 ◽  
Vol 1 ◽  
pp. 176-185
Author(s):  
S G Yiase ◽  
S O Adejo ◽  
S T Iningev
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Dilution Method ◽  
Spectroscopic Techniques ◽  
Metal Chlorides ◽  
Activity Screening ◽  
Antibacterial And Antifungal ◽  
Broth Dilution

Mn(II) and Co(II) complexes were prepared by reaction of the metal chlorides with acetylacetone in ammonical aqueous medium. The metal complexes were prepared in order to investigate their antimicrobial activity on some selected pathogens. The characterisation of the complexes was on the basis of various spectroscopic techniques like infrared and ultraviolet studies. The compounds were subjected to antimicrobial activity screening using serial broth dilution method. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal/Fungicidal Concentration (MBC/MFC) were determined. Mn(II) complex has shown significantly both antibacterial and antifungal activity with a MIC of 1.25 μg/mL while Co(II) complex was noticeable for antifungal activity at the same concentration. Whereas Mn(II) acetylacetonate is a more potent bactericide while Co(II) acetylacetonate is a more potent fungicide, both with MBC/MFC value of 2.5 μg/mL. Antimicrobial agent of the ligand has enhanced on complexation with Mn(II) and Co(II) ions. Though, the potency of the prepared antibiotics on the tested microbes is less compared to the standard drugs (Ciprofloxacin and Fulcin).

scholarly journals Antifungal Activity of Crude Extract Compound from Rhus verniciflua Against Anthracnose Fungi (Collectotrichum spp.) of Red-Pepper

2012 ◽  
Vol 31 (1) ◽  
pp. 60-67 ◽  
Author(s):  
Chi-Hyoun Song ◽  
Jong-Bae Chung ◽  
Byoung-Ryong Jeong ◽  
Se-Young Park ◽  
Yong-Se Lee
Keyword(s):  
Antifungal Activity ◽  
Crude Extract ◽  
Red Pepper ◽  
Rhus Verniciflua

scholarly journals Synthesis and Bio-Evaluation of Natural Butenolides-Acrylate Conjugates

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1304 ◽  
Author(s):  
Longzhu Bao ◽  
Shuangshuang Wang ◽  
Di Song ◽  
Jingjing Wang ◽  
Xiufang Cao ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Antifungal Activity ◽  
13C Nmr ◽  
Pathogenic Fungi ◽  
Phytopathogenic Fungi ◽  
Gibberella Zeae ◽  
Ionization Mass ◽  
Activity Screening ◽  
In Vitro Antifungal Activity

A series of novel 3-aryl-4-hydroxy-2(5H) furanone-acrylate hybrids were designed and synthesized based on the natural butenolides and acrylates scaffolds. The structures of the prepared compounds were characterized by 1H-NMR, 13C-NMR and electrospray ionization mass spectrometry (ESI-MS), and the bioactivity of the target compounds against twelve phytopathogenic fungi was investigated. The preliminary in vitro antifungal activity screening showed that most of the target compounds had moderate inhibition on various pathogenic fungi at the concentration of 100 mg·L−1, and presented broad-spectrum antifungal activities. Further studies also indicated that compounds 7e and 7k still showed some inhibitory activity against Pestallozzia theae, Sclerotinia sclerotiorum and Gibberella zeae on rape plants at lower concentrations, which could be optimized as a secondary lead for further research.

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