scholarly journals Transgenic shoots and plants as a source of natural phytochemical products

2014 ◽  
Vol 69 (2) ◽  
pp. 131-136 ◽  
Author(s):  
Katarzyna Floryanowicz-Czekalska ◽  
Halina Wysokińska
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Secondary Metabolites ◽  
Genetic Engineering ◽  
Bioactive Compounds ◽  
In Vitro Cultures ◽  
Background Information ◽  
Transformation Techniques ◽  
Aerial Parts ◽  
Whole Plants

Genetic engineering has allowed the production of plants and in vitro cultures with an altered content of secondary metabolites. In the present work it is hoped to give some detailed background information on obtaining bioactive compounds based on the use of genetically transformed shoots and the whole plants. <em>Agrobacterium tumefaciens</em>-mediated shoots have recently been a matter of great interest as a source of chemicals synthesized in the aerial parts of plants. The possibilities for the future exploitation of <em>Agrobacterium tumefaciens</em> transformation techniques are enormous. However, we need more knowledge of genes and enzymes controlling secondary metabolic synthesis.

Caffeoylquinic Acids with Potential Biological Activity from Plant In vitro Cultures as Alternative Sources of Valuable Natural Products

2020 ◽  
Vol 26 (24) ◽  
pp. 2817-2842
Author(s):  
Ewa Skała ◽  
Joanna Makowczyńska ◽  
Joanna Wieczfinska ◽  
Tomasz Kowalczyk ◽  
Przemysław Sitarek
Keyword(s):  
Secondary Metabolites ◽  
Cell Suspension ◽  
Plant Species ◽  
Plant Secondary Metabolites ◽  
Cell Suspension Cultures ◽  
In Vitro Cultures ◽  
Transformed Roots ◽  
Transgenic Roots ◽  
Plant In Vitro Cultures

Background: For a long time, the researchers have been looking for new efficient methods to enhance production and obtain valuable plant secondary metabolites, which would contribute to the protection of the natural environment through the preservation of various plant species, often rare and endangered. These possibilities offer plant in vitro cultures which can be performed under strictly-controlled conditions, regardless of the season or climate and environmental factors. Biotechnological methods are promising strategies for obtaining the valuable plant secondary metabolites with various classes of chemical compounds including caffeoylquinic acids (CQAs) and their derivatives. CQAs have been found in many plant species which are components in the daily diet and exhibit a wide spectrum of biological activities, including antioxidant, immunomodulatory, antihypertensive, analgesic, anti-inflammatory, hepato- and neuroprotective, anti-hyperglycemic, anticancer, antiviral and antimicrobial activities. They have also been found to offer protection against Alzheimer’s disease, and play a role in weight reduction and lipid metabolism control, as well as modulating the activity of glucose-6-phosphatase involved in glucose metabolism. Methods: This work presents the review of the recent advances in use in vitro cultures of various plant species for the alternative system to the production of CQAs and their derivatives. Production of the secondary metabolites in in vitro culture is usually performed with cell suspension or organ cultures, such as shoots and adventitious or transformed roots. To achieve high production of valuable secondary metabolites in in vitro cultures, the optimization of the culture condition is necessary with respect to both biomass accumulation and metabolite content. The optimization of the culture conditions can be achieved by choosing the type of medium, growth regulators or growth conditions, selection of high-productivity lines or culture period, supplementation of the culture medium with precursors or elicitor treatments. Cultivation for large-scale in bioreactors and genetic engineering: Agrobacterium rhizogenes transformation and expression improvement of transcriptional factor or genes involved in the secondary metabolite production pathway are also efficient strategies for enhancement of the valuable secondary metabolites. Results: Many studies have been reported to obtain highly productive plant in vitro cultures with respect to CQAs. Among these valuable secondary metabolites, the most abundant compound accumulated in in vitro cultures was 5-CQA (chlorogenic acid). Highly productive cultures with respect to this phenolic acid were Leonurus sibiricus AtPAP1 transgenic roots, Lonicera macranthoides and Eucomia ulmoides cell suspension cultures which accumulated above 20 mg g-1 DW 5-CQA. It is known that di- and triCQAs are less common in plants than monoCQAs, but it was also possible to obtain them by biotechnological methods. Conclusion: The results indicate that the various in vitro cultures of different plant species can be a profitable approach for the production of CQAs. In particular, an efficient production of these valuable compounds is possible by Lonicera macranthoides and Eucomia ulmoides cell suspension cultures, Leonurus sibiricus transformed roots and AtPAP1 transgenic roots, Echinacea angustifolia adventitious shoots, Rhaponticum carthamoides transformed plants, Lavandula viridis shoots, Sausera involucrata cell suspension and Cichorium intybus transformed roots.

Comparative Investigation on Formation and Accumulation of Rare Phenylpropanoids in Plants and in vitro Cultures of Pimpinella major

1990 ◽  
Vol 45 (6) ◽  
pp. 602-606 ◽  
Author(s):  
B. Merkel ◽  
J. Reichling
Keyword(s):  
Callus Culture ◽  
Nutrient Medium ◽  
Callus Cultures ◽  
Comparative Investigation ◽  
In Vitro Cultures ◽  
Liquid Nutrient Medium ◽  
Whole Plants ◽  
Plant Seedlings

Abstract Unorganized callus and leaf/root-differentiating callus cultures of Pimpinella major have been established in liquid nutrient medium. Their capacity to accumulate rare phenylpropanoids such as epoxy-pseudoisoeugenol tiglate, epoxy-anol tiglate and anol tiglate was compared with that of seedlings and whole plants. The unorganized callus cultures were not able to accumulate any phenylpropanoids. In comparison, the leaf/root-differentiating callus culture promoted the accumulation of epoxy-pseudoisoeugenol tiglate (up to 90 mg/100 g fr.wt.) but not that of anol-derivatives. The accumulated amount of EPT in PMD-SH was comparable with that in plant seedlings.

scholarly journals MTMS-Based Aerogel Constructs for Immobilization of Plant Hairy Roots: Effects on Proliferation of Rindera graeca Biomass and Extracellular Secretion of Naphthoquinones

2021 ◽  
Vol 12 (1) ◽  
pp. 19
Author(s):  
Bartosz Nowak ◽  
Mateusz Kawka ◽  
Kamil Wierzchowski ◽  
Katarzyna Sykłowska-Baranek ◽  
Maciej Pilarek
Keyword(s):  
Polyurethane Foam ◽  
Bioactive Compounds ◽  
Hairy Roots ◽  
Reference System ◽  
Culture System ◽  
In Vitro Cultures ◽  
Fresh Mass ◽  
Extracellular Secretion ◽  
High Level

Unique biosynthetic abilities revealed by plants determine in vitro cultures of hairy roots as a suitable source of pharmaceutically relevant bioactive compounds. The basic aim of the study was to examine the applicability of aerogel composed of methyltrimethoxysilane (MTMS) for immobilization of Rindera graeca hairy roots by identifying quantitative effects of biomass proliferation and naphthoquinones extracellular secretion in the aerogel-supported culture system. R. graeca hairy roots were simultaneously cultured for 28-days, as (i) nonimmobilized biomass (reference system), (ii) biomass immobilized on macroporous polyurethane foam (PUF), (iii) biomass with disintegrated MTMS aerogel, (iv) biomass immobilized on polypropylene (PP) fibers (as control), and (v) biomass immobilized on monolithic PP-reinforced MTMS aerogel. MTMS aerogel exhibited high level of biocompatibility toward R. graeca hairy roots which grew into the structure of monolithic aerogel-based constructs. Monolithic MTMS-based constructs significantly promoted the proliferation of hairy roots, resulting in 55% higher fresh mass than the reference system. The highest level of naphthoquinones productivity, i.e., 653 µg gDW−1, was noted for PUF-supported culture system.

Secondary Metabolites Accumulation and Production Through In Vitro Cultures

2018 ◽  
pp. 131-186
Author(s):  
Hussien M. Daffalla ◽  
Azza Migdam Elsheikh
Keyword(s):  
Secondary Metabolites ◽  
In Vitro Cultures

scholarly journals Diversity of Secondary Metabolites in Roots from Conium maculatum L.

Plants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 939
Author(s):  
Remigius Chizzola ◽  
Ulrike Lohwasser
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
High Variability ◽  
Gas Chromatography Mass Spectrometry ◽  
Conium Maculatum ◽  
Aerial Parts ◽  
Tap Root ◽  
Toxic Plant

Background: Conium maculatum is known as highly toxic plant, due to piperidine alkaloids present in the aerial parts. In a first attempt, in various tap root samples, however, alkaloids could not be detected. The present study describes active compounds in the tap roots from 16 populations harvested at maturity. The compounds were extracted with dichloromethane from root pieces of single plants and analyzed by gas chromatography–mass spectrometry. Ten bioactive compounds were evaluated: five furocoumarins, two prenylated coumarins, two aliphatic C17-polyacetylenes and the phenylpropanoid elemicin. A high variability could be observed, the highest concentrations were measured for falcarindiol, xanthotoxin and isopimpinellin, the lowest for elemicin. In sum C. maculatum roots contained comparable amounts of compounds that are characteristic for Apiaceae, and also occur in vegetables as carrots, parsnip, parsley or celeriac.

Secondary metabolites from the aerial parts of Sideritis germanicopolitana and their in vitro enzyme inhibitory activities

2019 ◽  
pp. 1-4 ◽  
Author(s):  
Hasan Kırmızıbekmez ◽  
Murat Erdoğan ◽  
Norbert Kúsz ◽  
Nursenem Karaca ◽  
Umur Erdem ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Aerial Parts ◽  
Inhibitory Activities

Elicitation of secondary metabolites in in vitro cultures of Ammi majus L.

2003 ◽  
Vol 33 (5) ◽  
pp. 565-568 ◽  
Author(s):  
Izabela Staniszewska ◽  
Aleksandra Królicka ◽  
Edmund Maliński ◽  
Ewa Łojkowska ◽  
Janusz Szafranek
Keyword(s):  
Secondary Metabolites ◽  
In Vitro Cultures ◽  
Ammi Majus

scholarly journals Phytochemistry and Pharmacology of Thymus broussonetii Boiss

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Hanae Naceiri Mrabti ◽  
Latifa Doudach ◽  
Naoual El Menyiy ◽  
Mohammed Bourhia ◽  
Ahmad Mohammad Salamatullah ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Essential Oils ◽  
Skin Diseases ◽  
Biological Activities ◽  
Aerial Parts ◽  
Toxicological Studies ◽  
Toxicological Investigation ◽  
Antioxidant Effects

Thymus broussonetii Boiss (T. broussonetii) is a rare medicinal and aromatic plant. It is widely used in traditional medicine to treat several diseases, including diarrhea, fever, cough, irritation, skin diseases, rheumatism, respiratory ailments, influenza, and digestion problems. In this review, we have critically summarized previous data on T. broussonetii about its phytochemistry, botanical and geographical distribution, toxicological investigation, and pharmacological properties. Using scientific research databases such as Wiley Online, SciFinder, ScienceDirect, PubMed, SpringerLink, Web of Science, Scopus Wiley Online, and Google Scholar, the data on T. broussonetii were collected and discussed. The presented data regrouped bioactive compounds and biological activities of T. broussonetii. The findings of this work showed that essential oils and extracts of T. broussonetii exhibited numerous pharmacological activities (in vitro and in vivo), particularly antibacterial, antifungal, antioxidant, anticancer, anti-inflammatory, insecticidal, antipyretic, antinociceptive, and immunological and behavioral effects. While toxicological studies of T. broussonetii essential oils and extracts are lacking, modern scientific tools revealed the presence of different classes of secondary metabolites such as terpenoids, alkaloids, flavonoids, tannins, coumarins, quinones, carotenoids, and steroids. T. broussonetii essential oils, especially from the aerial parts, exhibited potent antibacterial, antifungal, and antioxidant effects. An in-depth toxicological investigation is needed to validate the efficacy and safety of T. broussonetii extracts and essential oils and their secondary metabolites. However, further pharmacokinetic and pharmacodynamic studies should be performed to validate its bioavailability.

Antibacterial activity of the secondary metabolites from in vitro cultures of Drosera aliciae

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
A Krolicka ◽  
A Szpitter ◽  
M Maci ąg ◽  
E Biskup ◽  
E Gilgenast ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
In Vitro Cultures
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