scholarly journals Effect of Beauveria bassiana and Metarhizium anisopliae on different stages of Phlebotomus papatasi (Diptera: Psychodidae)

2021 ◽  
Vol 90 (4 - Ahead of print) ◽  
pp. 175-193
Author(s):  
Maha Moustafa Ahmed ◽  
Heba Yehia Mady ◽  
Amira Hassan El Namaky

The sand fly, Phlebotomus papatasi (Scopoli, 1786) (Diptera: Psychodidae), is the main vector of Leishmania major Yakimoff and Schokhor, 1914, the causative agent of zoonotic cutaneous leishmaniasis North Africa, the Middle East, and North Sinai. The purpose of this study was to determine the effect of fungi on P. papatasi larvae, pupae, and adults using light microscopic analysis, scanning electron microscope (SEM), and histopathological studies. Specifically, larvae, pupae, and adult P. papatasi were infected with the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae. Scanning electron microscope and histopathological methods were used to investigate the destructive impact of the fungi on the external and internal structures of P. papatasi. The results revealed propagation of the conidia on the cuticles of all P. papatasi life stages, including on the compound eyes, leg setae, thorax, wings, and abdomen of the adults. Histological sections of the control and treated larvae, pupae, and adults showed many alterations and malformations in the body and tissues of all life stages after 72 h. These results demonstrated that B. bassiana was more effective than M. anisopliae as a biological control of phlebotomine sand flies. Further studies to determine the best methods for delivery and application in the diverse ecological settings of the various leishmaniasis vectors are recommended.

Author(s):  
W. Brünger

Reconstructive tomography is a new technique in diagnostic radiology for imaging cross-sectional planes of the human body /1/. A collimated beam of X-rays is scanned through a thin slice of the body and the transmitted intensity is recorded by a detector giving a linear shadow graph or projection (see fig. 1). Many of these projections at different angles are used to reconstruct the body-layer, usually with the aid of a computer. The picture element size of present tomographic scanners is approximately 1.1 mm2.Micro tomography can be realized using the very fine X-ray source generated by the focused electron beam of a scanning electron microscope (see fig. 2). The translation of the X-ray source is done by a line scan of the electron beam on a polished target surface /2/. Projections at different angles are produced by rotating the object.During the registration of a single scan the electron beam is deflected in one direction only, while both deflections are operating in the display tube.


1972 ◽  
Vol 3 (3) ◽  
pp. 181-188 ◽  
Author(s):  
Christine Dahl

AbstractA method for preparation of alcohol-preserved culicid larvae for Scanning Electron Microscope (SEM) studies is described. It is based on dehydration by ethanol-xylol and fast evaporation of xylol in +8o° C. for ten minutes. For taxonomic studies such as examination of pecten teeth, comb scales and microtrichiae in magnifications up to 6oooX the method is suitable. For studies of receptor structures on hair-tufts and microstructures of the body integument alcohol preserved material is less satisfactory. The microstructure of the comb scales is figured and their function discussed. Differences in the ultrastructure of the abdominal hair-tufts are pointed out.


Author(s):  
C. Newton ◽  
W. T. W. Potts

Magnesium/chlorine and sulphur/chlorine ratios have been measured in the body fluids of some planktonic organisms by x-ray emission analysis of frozen hydrated specimens in a scanning electron microscope. Homarus vulgaris (Anthropoda: Decapoda) larvae excluded Mg2+ and SO42-ions from the haemolymph, but to a lesser extent than does the adult lobster. Bipinnaria larvae of Asterias (Echinodermata) excluded Mg2+ and SO42-ions from the coelomic fluid. Obelia medusae excluded Mg2+ and SO42-ions from the mesogloea but concentrate them in the gastrovascular system. The high concentration of sulphate in the gastrovascular fluid of medusae has been confirmed by rhodizonate titration in Cyanea and Rhizostoma jellyfish. Some implications of magnesium and sulphate regulation are discussed.


Zootaxa ◽  
2004 ◽  
Vol 397 (1) ◽  
pp. 1 ◽  
Author(s):  
JUNGSUN YOO ◽  
JOOPIL KIM ◽  
HOZUMI TANAKA

Alopecosa volubilis n. sp. is described from Korea and is differentiated from other species in Alopecosa Simon, 1885 by morphological characteristics, such as three retromarginal teeth of chelicera, smaller body size, and the presence of a distinct tip of the median apophysis. The pedipalpal sclerites and somatic characters of two similar species, A. moriutii Tanaka, 1985 and A. hokkaidensis Tanaka, 1985, are compared with those of the new species. An illustration of the body and scanning electron microscope (SEM) pictures of the pedipalpal organ are provided.


2017 ◽  
Vol 22 (8) ◽  
pp. 1181
Author(s):  
Jueyingqi Jiang ◽  
Yannan Zhang ◽  
Dandan Guo ◽  
Jianping Zhang ◽  
Jing Chen

Tetranychus turkestani is a serious pest of cotton, corn, vegetables, fruit trees and forests in Xinjiang. All life stages of T. turkestani were observed using both light and scanning electron microscope. The egg is smooth and spherical. The eupathidial spinnerets of larva and protonymph are different from that of the deutonymph and adult in shape. The spinneret of adult female is obviously thicker and larger than that in male. The palpfemoral seta of adult male is a short, stout, spine-like process. T. turkestani has fourteen pairs of dorsal setae. The number of ventral setae increases regularly in number in each molt. The leg chaetotaxy has a great change from larva to deutonymph.


Author(s):  
H. V. Smith ◽  
R. N. Titchener

SynopsisPhotographs were displayed of the mouthparts of some important arthropod parasites of British livestock as viewed with a scanning electron microscope. Ectoparasites included in the demonstration were Damalinia ovis, the body louse of sheep; Haematopinus eurysternus, the short-nosed cattle louse; Ceratophyllus gallinae, the hen flea; Melophagus ovinus, the sheep ked and Ixodes ricinus, the sheep tick. Photographs of livestock infested with these parasites were also displayed.


2017 ◽  
Vol 19 (2) ◽  
pp. 76 ◽  
Author(s):  
Η.Α. Vahedi ◽  
F. Gholami Mahfar

The morphology of the multilocular disc-pores from the anterior part of the body (abdominal segments I-III and all thoracic segments, dorso-venterally) of two species of Porphyrophora (P. tritici and P. cynodontis: Hemiptera: Coccoidea: Margarodidae) were examined using a scanning electron microscope. The multilocular disc-pores of both species have 1 or 2 (rarely 3) rings of evenly or unevenly distributed loculi but the more central rings are almost always incomplete. Each outer ring of the more anterior disc-pores of P. tritici was complete, with 5- 13 loculi, and the inner ring had 0-4 unevenly distributed loculi. Each disc-pore of P. cynodontis had a complete outer ring of 6-9 evenly distributed loculi and an inner ring with only 0-1 loculi. A bright central zone is absent in most cases. These two species are similar in that both have a dense network of small, globular projections throughout the derm surface. These observations are discussed in relation to those of light microscope studies.


2018 ◽  
Vol 86 (2) ◽  
pp. 89-93 ◽  
Author(s):  
Cristina Mondello ◽  
Antonio Micali ◽  
Luigi Cardia ◽  
Antonina Argo ◽  
Stefania Zerbo ◽  
...  

Diagnosis of death by electrocution may be difficult when electric marking is not visible or unclear. Accordingly, the body of a man who appeared to have died from accidental electrocution was carefully forensically analysed. Macroscopic and microscopic analysis of the current mark was carried out using a variable-pressure scanning electron microscope equipped with energy dispersive X-ray microanalyser to highlight skin metallisation, indicating the presence of iron and zinc. The histological findings of electrocution myocardial damage were supported by the results of biochemical analysis which demonstrated the creatine kinase-MB and cardiac troponin I elevation. The effects of electric current flow were also highlighted by perforations of endothelial surface of a pulmonary artery using scanning electron microscope, and all the results were analysed by the main tools suggested in the literature.


1980 ◽  
Vol 58 (3) ◽  
pp. 382-385 ◽  
Author(s):  
A. Redlich

Gordius attoni sp.n. (Nematomorpha, Gordiidae) is described using a scanning electron microscope (SEM) and conventional methods. The presence of white spots, the postcloacal crescent on the male caudal end, and the shallow interareolar furrows devoid of pores and bristles as seen in SEM are characteristic of the genus Gordius. The species is characterized by its spindle shape and well defined polygonal areoles, which completely cover the body surface. Spinules, sparsely distributed mainly on the periphery of the areoles, are apparent in SEM. Adults have been found north of 51.5° extending beyond the Arctic circle from the Western Yukon to Chesterfield Inlet of Hudson's Bay.


Author(s):  
R. E. Ferrell ◽  
G. G. Paulson

The pore spaces in sandstones are the result of the original depositional fabric and the degree of post-depositional alteration that the rock has experienced. The largest pore volumes are present in coarse-grained, well-sorted materials with high sphericity. The chief mechanisms which alter the shape and size of the pores are precipitation of cementing agents and the dissolution of soluble components. Each process may operate alone or in combination with the other, or there may be several generations of cementation and solution.The scanning electron microscope has ‘been used in this study to reveal the morphology of the pore spaces in a variety of moderate porosity, orthoquartzites.


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