scholarly journals PERFIL PROTEICO DO LÍQUIDO FOLICULAR COLETADO DE OVÁRIOS EM DIFERENTES FASES DO CICLO ESTRAL DE BOVINOS

2012 ◽  
Vol 8 (2) ◽  
pp. 65-74 ◽  
Author(s):  
Renato Weller Ribeiro ◽  
Francislaine Aneliza Garcia Santos ◽  
Caliê Castilho ◽  
José Giometti ◽  
Luciana Machado Guaberto ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Estrous Cycle ◽  
Follicular Fluid ◽  
Protein Profile ◽  
Oocyte Quality ◽  
Morphologic Characteristics

The female gamete’s microenvironment is the follicular fluid. The proteins in the follicular fluid must interfere in the oocyte quality. The objective of this study was to verify the protein profile in the follicular fluid of different phases of bovine estrous cycle. The follicular fluid was taken from follicles of bovine ovaries obtained at slaughterhouse. The ovaries were classified into five different phases of estrous cycle, according to presence or absence of corpus luteum (CL) and the morphologic characteristics. Pools of follicular fluid were collected from follicles ranging from 2 to 7mm in diameter from ovaries in five different phases of estrous cycle (1=initial CL, hemorrhagic; 2= developing CL; 3= mature CL; 4=regressing CL and 5=absence CL). The protein profile was evaluated by poliacrilamide eletrophoresis and determined by percentages of samples from each phase. The polypeptides with molecular weigh of 35, 32, 30 and 18 KDa changed considerably in samples from different phases of estrous cycle. The polypeptidewith the same molecularweight ofIGFBP-2 is more observedin samples from follicular fluid in the end of estrous cycle. The most polypeptides were observed insmallfollicles from ovaries with regressing CL

scholarly journals Gene expression of glucose transporter (GLUT) 1, 3 and 4 in bovine follicle and corpus luteum

2006 ◽  
Vol 188 (1) ◽  
pp. 111-119 ◽  
Author(s):  
H Nishimoto ◽  
R Matsutani ◽  
S Yamamoto ◽  
T Takahashi ◽  
K-G Hayashi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Corpus Luteum ◽  
Estrous Cycle ◽  
Granulosa Cells ◽  
Follicular Fluid ◽  
Glucose Transporter ◽  
Ovarian Activity ◽  
Notable Increase ◽  
Follicular Size ◽  
Theca Interna

Glucose is the main energy substrate in the bovine ovary, and a sufficient supply of it is necessary to sustain the ovarian activity. Glucose cannot permeate the plasma membrane, and its uptake is mediated by a number of glucose transporters (GLUT). In the present study, we investigated the gene expression of GLUT1, 3 and 4 in the bovine follicle and corpus luteum (CL). Ovaries were obtained from Holstein × Japanese Black F1 heifers. Granulosa cells and theca interna layers were harvested from follicles classified into five categories by their physiologic status: follicular size (≥ 8.5 mm: dominant; < 8.5 mm: subordinate), ratio of estradiol (E2) to progesterone in follicular fluid (≥ 1: E2 active;<1: E2 inactive), and stage of estrous cycle (luteal phase, follicular phase). CL were also classified by the stage of estrous cycle. Expression levels of GLUT1, 3 and 4 mRNA were quantified by a real-time PCR. The mRNA for GLUT1 and 3 were detected in the bovine follicle and CL at comparable levels to those in classic GLUT-expressing organs such as brain and heart. Much lower but appreciable levels of GLUT4 were also detected in these tissues. The gene expression of these GLUT showed tissue- and stage-specific patterns. Despite considerable differences in physiologic conditions, similar levels of GLUT1, 3 and 4 mRNA were expressed in subordinate follicles as well as dominant E2-active follicles in both luteal and follicular phases, whereas a notable increase in the gene expression of these GLUT was observed in dominant E2-inactive follicles undergoing the atretic process. In these follicles, highly significant negative correlations were observed between the concentrations of glucose in follicular fluid and the levels of GLUT1 and 3 mRNA in granulosa cells, implying that the local glucose environment affects glucose uptake of follicles. These results indicate that GLUT1 and 3 act as major transporters of glucose while GLUT4 may play a supporting role in the bovine follicle and CL.

scholarly journals Effect of some Nigerian traditional alcoholic beverages on the estrous cycle and histological assessment of the ovaries and uterus of albino rats

2021 ◽  
Vol 14 (1) ◽  
pp. 027-036
Author(s):  
Olusayo Moritiwon ◽  
Timothy Olugbenga Ogundeko ◽  
James Bitrus ◽  
David Oyebode ◽  
Olufunmilayo Ibiyemi Abobarin
Keyword(s):  
Corpus Luteum ◽  
Estrous Cycle ◽  
Ovarian Follicle ◽  
Alcoholic Beverages ◽  
Albino Rats ◽  
Histological Image ◽  
Follicular Cyst ◽  
Regular Consumption ◽  
Alcoholic Drinks ◽  
Corpus Luteum Cyst

Alcohol related health challenges have lingered over the years. Such associated with locally brewed alcoholic beverages have received little attention. The urgency to explore this becomes imperative. This study aimed at evaluating the effect of regular consumption of local alcoholic beverages on the estrous cycle and histological image of the ovaries and uterus thus fertility of female albino rats. Thirty screened female albino rats weighing 180-220g were divided into five groups and daily administered each with 10ml/kg of pito, burukutu, ogogoro, goskolo and 0.5ml/kg normal saline respectively for 21 days. Effect of the beverages on the estrous cycle as well as histopathological evaluation was carried out on the isolated ovaries and uterus. Results showed significant increase proestrous phase of the estrous cycle with ingestion of pito, burukutu, ogogorogo and goskolo. Also the histology of the ovary was basically without obvious pathological changes with pito, while there was alteration of histological parameters by burukutu, marked with formation of fibrosis, corpus luteum cyst resulting immature ovarian follicle, ogogoro marked with formation of fibrosis, corpus luteum cyst resulting immature ovarian follicle and goskolo marked with formation of multiple follicular cyst resulting in complete collapse of the ovarian section. The effect of traditional alcoholic beverages revealed the classical effects of alcoholic drinks by way of significant alteration in the estrous cycle of albino rats with marked alteration of the histological architecture of ovarian tissues. Pito, burukutu, ogogoro, and goskolo have a reprotoxic effect on the ovaries and uterus thus a deleterious effect on fertility of female albino rats.

scholarly journals Effect of Follicular Fluid Oxidative Stress on Subsequent Oocyte Quality and Embryo Development

2016 ◽  
Vol 26 (1) ◽  
pp. 1-6
Author(s):  
Özgür BİGE ◽  
Bülent GÜLEKLİ ◽  
Ahmet DEMİR ◽  
Funda GÖDE ◽  
Semra KOÇTÜRK ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Embryo Development ◽  
Follicular Fluid ◽  
Oocyte Quality

Studies on Ovarian Follicular Dynamics and Steroid Profiles in Sahiwal Cattle

2021 ◽  
Author(s):  
Vinny Dodiyar ◽  
Parkash Singh Brar ◽  
Narinder Singh ◽  
Mrigank Honparkhe
Keyword(s):  
Corpus Luteum ◽  
Estrous Cycle ◽  
Maximum Diameter ◽  
Endocrine Regulation ◽  
Dominant Follicle ◽  
Estrous Cycles ◽  
Significant Difference ◽  
Sahiwal Cattle ◽  
Sahiwal Cows ◽  
Follicular Dynamics

Background: Understanding of ovarian follicular dynamics and endocrine regulation is essential to design and use interventions to optimize reproductive efficiency. Bos indicus and Bos taurus cows have some differences in their follicular dynamics and ovarian steroidal hormones. The present study was planned to understand follicular dynamics vis-a-vis ovarian steroids profiles in Sahiwal cattle. Methods: The study was conducted in normal cyclic, pluriparous, non-lactating Sahiwal cows (n=7). Trans-rectal ultrasonography was performed to record the location, size, number of follicles and size of corpus luteum (CL) from the beginning, till the end of experiment. The dataset was used to characterize the follicular wave emergence, growth, regression, time of selection and ovulation of the dominant follicle in each cow. Result: Three Sahiwal cows showed two wave and four cows showed three wave estrous cycles with mean interovulatory length of 20.33±0.33 and 22.50±0.28 days, respectively. No significant difference was recorded in the maximum diameter of corpus luteum, P4 and E2 plasma levels between 2-wave and 3-wave estrous cycles. The peak progesterone values of 6.00±0.91 and 6.2±1.2 ng/ml and peak estradiol values of 15.83±0.60 and 14.31±0.44 ng/ml were recorded in 2-wave and 3-wave estrous cycle, respectively. The results showed that Sahiwal cows had 2-wave and 3-wave estrous cycle and the 3-wave estrous cycle had comparatively longer inter-ovulatory period and smaller maximum diameter of second wave dominant follicle than 2-wave estrous cycle.

Influence of Induced Uterine Infection at Various Stages of the Estrous Cycle on the Corpus Luteum of the Ewe

1969 ◽  
Vol 29 (4) ◽  
pp. 616-618 ◽  
Author(s):  
T. H. Brinsfield ◽  
D. K. Higginbotham ◽  
H. W. Hawk
Keyword(s):  
Corpus Luteum ◽  
Estrous Cycle ◽  
Uterine Infection

Comparison of bovine uterine horns for progesterone and oxytocin levels with bilateral corpus luteum

1996 ◽  
Vol 76 (3) ◽  
pp. 463-464 ◽  
Author(s):  
W. A. Cerbito ◽  
M. P. B. Wijayagunawardane ◽  
M. Takagi ◽  
K. Sato ◽  
A. Miyamoto ◽  
...  
Keyword(s):  
Key Words ◽  
Corpus Luteum ◽  
Estrous Cycle ◽  
Luteal Phase ◽  
Uterine Horn ◽  
Corpora Lutea ◽  
Uterine Tissue ◽  
Tissue Samples ◽  
Significant Difference ◽  
The Right

Bovine uterine horns with both ovaries containing a corpus luteum (CL) were compared for progesterone (P4) and oxytocin (OT) concentrations during the luteal phase of the estrous cycle. Uterine tissue samples from five Holstein cows with bilateral CL obtained from the slaughterhouse were used for this study. No significant difference was observed in P4 and OT levels in the right and left horns with corpora lutea in both ovaries. The data clearly indicate that both sides of the uterine horn having a functional CL are exposed to similar levels of P4 and OT, supporting the hypothesis that luteal products are delivered locally to the uterus. Key words: Progesterone, oxytocin, uterine horn, bilateral, corpus luteum, cow

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