scholarly journals Gene expression of glucose transporter (GLUT) 1, 3 and 4 in bovine follicle and corpus luteum

2006 ◽  
Vol 188 (1) ◽  
pp. 111-119 ◽  
Author(s):  
H Nishimoto ◽  
R Matsutani ◽  
S Yamamoto ◽  
T Takahashi ◽  
K-G Hayashi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Corpus Luteum ◽  
Estrous Cycle ◽  
Granulosa Cells ◽  
Follicular Fluid ◽  
Glucose Transporter ◽  
Ovarian Activity ◽  
Notable Increase ◽  
Follicular Size ◽  
Theca Interna

Glucose is the main energy substrate in the bovine ovary, and a sufficient supply of it is necessary to sustain the ovarian activity. Glucose cannot permeate the plasma membrane, and its uptake is mediated by a number of glucose transporters (GLUT). In the present study, we investigated the gene expression of GLUT1, 3 and 4 in the bovine follicle and corpus luteum (CL). Ovaries were obtained from Holstein × Japanese Black F1 heifers. Granulosa cells and theca interna layers were harvested from follicles classified into five categories by their physiologic status: follicular size (≥ 8.5 mm: dominant; < 8.5 mm: subordinate), ratio of estradiol (E2) to progesterone in follicular fluid (≥ 1: E2 active;<1: E2 inactive), and stage of estrous cycle (luteal phase, follicular phase). CL were also classified by the stage of estrous cycle. Expression levels of GLUT1, 3 and 4 mRNA were quantified by a real-time PCR. The mRNA for GLUT1 and 3 were detected in the bovine follicle and CL at comparable levels to those in classic GLUT-expressing organs such as brain and heart. Much lower but appreciable levels of GLUT4 were also detected in these tissues. The gene expression of these GLUT showed tissue- and stage-specific patterns. Despite considerable differences in physiologic conditions, similar levels of GLUT1, 3 and 4 mRNA were expressed in subordinate follicles as well as dominant E2-active follicles in both luteal and follicular phases, whereas a notable increase in the gene expression of these GLUT was observed in dominant E2-inactive follicles undergoing the atretic process. In these follicles, highly significant negative correlations were observed between the concentrations of glucose in follicular fluid and the levels of GLUT1 and 3 mRNA in granulosa cells, implying that the local glucose environment affects glucose uptake of follicles. These results indicate that GLUT1 and 3 act as major transporters of glucose while GLUT4 may play a supporting role in the bovine follicle and CL.

scholarly journals Relaxin protein and gene expression in ovarian follicles of immature pigs

1998 ◽  
Vol 21 (2) ◽  
pp. 179-187 ◽  
Author(s):  
KM Ohleth ◽  
Q Zhang ◽  
CA Bagnell
Keyword(s):  
Gene Expression ◽  
Granulosa Cells ◽  
Follicular Fluid ◽  
Ovarian Follicle ◽  
Follicular Development ◽  
Ovarian Follicles ◽  
In Vitro Growth ◽  
Follicle Size ◽  
Theca Interna

Relaxin production by the ovarian follicle of gonadotropin-primed, prepubertal gilts is well documented. As far as we are aware, a source of relaxin in pig follicles, independent of gonadotropins, has not yet been reported. Therefore, the objective of this study was to determine whether relaxin is produced in porcine follicles in the absence of exogenous or cyclic gonadotropins. In immature pigs, immunoreactive relaxin was detected in fluids from small (1-3 mm), medium (4-5 mm) and large (>6 mm) follicles and localized to the theca interna of large follicles. Relaxin levels in follicular fluid significantly increased with follicle size (P<0.05). Relaxin mRNA was detected in whole small- and medium-sized follicles. In large follicles, the relaxin gene was expressed in thecal layers, but not granulosa cells. The abundance of relaxin transcript did not change with follicle size. In summary, relaxin protein and mRNA were detected in porcine follicles from immature animals, indicating that relaxin is produced in the porcine follicle in the absence of exogenous or cyclic gonadotropins. Relaxin's in vitro growth effects on porcine granulosa and theca cells support this follicular relaxin as a growth modulator during porcine follicular development.

scholarly journals Expression of mRNAs for Pro-and Anti-Apoptotic Factors in Granulosa Cells and Follicular Fluid of Women Undergoing in Vitro Fertilization. A Pilot Study

2021 ◽  
Author(s):  
Jozsef Bodis ◽  
Endre Sulyok ◽  
Akos Varnagy ◽  
Viktória Prémusz ◽  
Krisztina Godony ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Vitro Fertilization ◽  
Mrna Expression ◽  
Granulosa Cells ◽  
Follicular Fluid ◽  
Vitro Fertilization ◽  
Expression Levels ◽  
The Impact ◽  
Apoptotic Factors

Abstract BackgroundThis observational clinical study evaluated the expression levels and predictive values of some apoptosis-related genes in granulosa cells (GCs) and follicular fluid (FF) of women undergoing in vitro fertilization (IVF).Methods GCs and FF were obtained at oocyte retrieval from 31 consecutive patients with heterogeneous infertility diagnosis (age: 34.3±5.8 years, body mass index: 24.02±3.12 kg/m2, duration of infertility: 4.2±2.1 years). mRNA expression of pro-apoptotic (BAX, CASP3, CASP8) and anti-apoptotic (BCL2, AMH, AMHR, FSHR, LHR, CYP19A1) factors was determined by quantitative RT-PCR using ROCHE LightCycler 480. Results No significant difference in GC or FF mRNA expression of pro- and anti-apoptotic factors could be demonstrated between IVF patients with (9 patients) or without (22 patients) clinical pregnancy. Each transcript investigated was detected in FF, but their levels were markedly reduced and independent of those in GCs. The number of retrieved oocytes was positively associated with GC AMHR (r=0.393, p=0.029), but the day of embryo transfer was negatively associated with GC LHR (r=-0.414, p=0.020) and GC FSHR transcripts (r=-0.535, p=0.002). When pregnancy positive group was analysed separately the impact of apoptosis- related gene expressions on some selected measures of IVF success could be observed. Strong positive relationship was found between gene expression levels of pro- and anti-apoptotic factors in GCs.ConclusionOur study provides only marginal evidences for the apoptosis dependence of IVF outcome and suggests that the apoptosis process induces adaptive increases of the anti-apoptotic gene expression to attenuate apoptosis and to protect cell survival.

scholarly journals INFLUENCE OF POSTWEANING GROWTH RATE ON PUBERTY AND OVARIAN ACTIVITY IN HEIFERS

1975 ◽  
Vol 55 (1) ◽  
pp. 93-100 ◽  
Author(s):  
J. J. DUFOUR
Keyword(s):  
Growth Rate ◽  
Corpus Luteum ◽  
Follicular Fluid ◽  
Initial Phase ◽  
Follicular Development ◽  
The Other ◽  
Ovarian Activity ◽  
Final Phase ◽  
Feeding Regime ◽  
Feeding Regimes

Thirty-six Holstein heifers were randomly assigned at the weight of 136 kg to two groups: a fast-growing (FR) and a moderate-growing (MR) feeding regime for an initial phase of 100 days, followed by a final phase ending with ovariectomy after puberty, during which half of each group was subjected to the other feeding regime. The effect at puberty of the FR was a nonsignificant reduction of 16.3 days in age, and a significant increase (P < 0.01) of 26.2 kg in weight when considering the feeding regimes of the initial phase of growth. When the same treatments were imposed during the final phase of growth, heifers on FR were 52.0 days younger at puberty (P < 0.01) and 10.0 kg lighter (P > 0.05) than heifers on MR. Follicular development, in terms of follicular fluid weight, and diameters of the largest and second largest follicles 15 days after estrus, was greater in the ovary bearing the corpus luteum than in the other ovary. The diameter of the second largest follicle was greater in heifers on FR when imposed in the final phase of growth. Injection of Vetrophin at the estrus preceding ovariectomy did not change the ovulation rate, but increased the percentage of small-sized follicles and decreased the percentage of medium-sized follicles. The number of corpora albicantia observed at ovariectomy indicated that 76.5% of the heifers ovulated prior to puberty without exhibiting a standing estrus. Of those exhibiting an estrus at first ovulation, 33.3% had a first estrous cycle of less than 10.0 days in length.

scholarly journals PERFIL PROTEICO DO LÍQUIDO FOLICULAR COLETADO DE OVÁRIOS EM DIFERENTES FASES DO CICLO ESTRAL DE BOVINOS

2012 ◽  
Vol 8 (2) ◽  
pp. 65-74 ◽  
Author(s):  
Renato Weller Ribeiro ◽  
Francislaine Aneliza Garcia Santos ◽  
Caliê Castilho ◽  
José Giometti ◽  
Luciana Machado Guaberto ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Estrous Cycle ◽  
Follicular Fluid ◽  
Protein Profile ◽  
Oocyte Quality ◽  
Morphologic Characteristics

The female gamete’s microenvironment is the follicular fluid. The proteins in the follicular fluid must interfere in the oocyte quality. The objective of this study was to verify the protein profile in the follicular fluid of different phases of bovine estrous cycle. The follicular fluid was taken from follicles of bovine ovaries obtained at slaughterhouse. The ovaries were classified into five different phases of estrous cycle, according to presence or absence of corpus luteum (CL) and the morphologic characteristics. Pools of follicular fluid were collected from follicles ranging from 2 to 7mm in diameter from ovaries in five different phases of estrous cycle (1=initial CL, hemorrhagic; 2= developing CL; 3= mature CL; 4=regressing CL and 5=absence CL). The protein profile was evaluated by poliacrilamide eletrophoresis and determined by percentages of samples from each phase. The polypeptides with molecular weigh of 35, 32, 30 and 18 KDa changed considerably in samples from different phases of estrous cycle. The polypeptidewith the same molecularweight ofIGFBP-2 is more observedin samples from follicular fluid in the end of estrous cycle. The most polypeptides were observed insmallfollicles from ovaries with regressing CL

Oxytocin determination in steroid producing tissues and in vitro production in ovarian follicles

1985 ◽  
Vol 109 (4) ◽  
pp. 530-536 ◽  
Author(s):  
D. Schams ◽  
Th. A. M. Kruip ◽  
R. Koll
Keyword(s):  
Corpus Luteum ◽  
Granulosa Cells ◽  
Follicular Fluid ◽  
De Novo ◽  
Prostate Gland ◽  
Seminal Vesicles ◽  
Corpora Lutea ◽  
In Vitro Production ◽  
Wet Weight

Abstract Immunoreactive oxytocin was measured in ovaries (corpus luteum and follicular fluid) and adrenals of cows, and in testes, seminal vesicles, prostate gland and adrenals of bulls. Secretion of oxytocin was further measured after culture of whole follicles, granulosa cells and theca tissue. Concentrations of oxytocin increased in corpora lutea of cycling cattle until mid-luteal phase (447 ± 93 ng/g wet weight) and decreased afterwards. Low concentrations were found in corpora lutea of pregnant animals (6 ± 3 ng/g wet weight). Follicular fluid contains some oxytocin (on average 42–108 pg/ml) but concentrations were significantly higher in the fluid of ovarian cysts (190 pg/ml). After culture of follicles the amount of oxytocin released into the medium increased indicating de novo synthesis. The granulosa cells were the main source of follicular oxytocin. Production increased during luteinization indicating that luteinization is an important step for the production of oxytocin in ovaries. Tissues of testes (65 ± 10 pg/g wet weight) and adrenals from cows (122 ± 39 pg/g wet weight) and bulls (111 ±2 pg/g wet weight) contained oxytocin but at much lower concentrations compared to corpus luteum tissue. About 10 times higher concentrations of oxytocin were measured in the adrenal medulla (717 ± 96 pg/g wet weight) compared to the cortex (72 ± 11 pg/g wet weight). Seminal vesicles and prostate gland contained no measurable amounts of oxytocin (< 5 pg/g wet weight).

scholarly journals ACTIVITY AND CONTENT OF SUPEROXIDEDISMUTASE IZOZYMES IN GRANULOSE CELLS FROM COW OVARY FOLLICLES

2020 ◽  
Vol 21 (2) ◽  
pp. 33-39
Author(s):  
Yu. V. Bodnar ◽  
N. V. Kuzmina ◽  
D. D. Ostapiv ◽  
S. W. Kawa ◽  
O. I. Chajkovska ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Corpus Luteum ◽  
Granulosa Cells ◽  
Follicular Fluid ◽  
Physiological State ◽  
Ovarian Follicles ◽  
Extracellular Proteins ◽  
Cytotoxic Action ◽  
Follicular Growth ◽  
Sod Activity

The activity and content of superoxide dismutase isoforms (SOD) in granulosa cells from cow ovarian follicles were studied for research after slaughter of cows ovaries were selected, which according to the physiological state were divided into groups: with "fresh" ovulation, at the site of the ovulated follicle there is a hole, no corpus luteum or diameter up to 5 mm, red color (n = 14); with "early corpus luteum", diameter 10-20 mm, color red or brown (n = 41); with “late corpus luteum", diameter 5–15 mm, color yellow (n = 32); "follicular growth", without the corpus luteum (n = 84). The ovaries of cows with small (<4 mm), medium (4 - 7 mm) and large (> 7 mm) follicles were used. Antral fluid was obtained from the follicles, from which granulosa cells were isolated. Cells were suspended according to the volume of follicular fluid in the medium Dulbeccos modified Eagle medium (DME) with the addition of estrus serum of cows, follicular fluid, insulin and heparin. In cell culture, protein concentration, activity, and superoxide dismutase isozymes were determined. It was found that granulosa cells are characterized by SOD activity - 12.4 ± 0.74 IU / mg protein (6.8 ± 1.72 - 19.8 ± 3.75 IU / mg protein). The activity of SOD in the culture of granulosa cells had 5–6 isoforms of the enzyme. It was found that isoforms at the site of localization are divided into cytosolic, mitochondrial and extracellular proteins of SOD. The cytosolic isoform were represented by 3 - 4, and mitochondrial and extracellular have one active protein of the enzyme. he activity of the enzyme and the content of isoforms depended on the size of the follicles from which the cells are removed and the physiological state of the ovaries. The studied indicators characterize the intensity of oxidative metabolism as a whole in cells and in their individual parts and organelles. For cultivation, it is advisable to select granulosa cells from ovarian follicles of "follicular growth" and "late corpus luteum" because they are characterized by consistently high activity of SOD, which protects intracellular components from the cytotoxic action of superoxide anion.

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