Due to the ozone depletion issue, R-502, which had long been used as the refrigerant of an ice cream refrigerator, has been replaced by R-404A. However, global warming potential (GWP) of R-404A is high, and thus, a replacement refrigerant is necessary in the long term. Natural refrigerants, such as R-290 or DME (dimethylether), could be a choice. In this study, an ice cream refrigerator cycle was optimized using R-290/DME mixture (mass fraction 65/35). The optimization was accomplished through a search for the proper refrigerant charge amount and the opening of the expansion valve. For the present ice cream refrigerator having 2.8[Formula: see text]L freezer volume, the optimum charge amount was 900[Formula: see text]g, and the optimum valve opening was [Formula: see text]120[Formula: see text]. At this configuration, the ice cream formation time was 3[Formula: see text] 6[Formula: see text] and COP was 2.0. The ice cream formation time was much shorter than when R-404A was used, and the COP was increased by more than 100%. For actual usage of the refrigerant, however, the flammability issue of the R-290/DME mixture should be cleared.