Measurement and Physiological Relevance of the Maximal Lipid Oxidation Rate During Exercise (LIPOXmax)

Women use more fat during endurance exercise as evidenced by a lower respiratory exchange ratio (RER). The contribution of intramyocellular lipid (IMCL) to lipid oxidation during endurance exercise is controversial, and studies investigating sex differences in IMCL utilization have found conflicting results. We determined the effect of sex on net IMCL use during an endurance exercise bout using an ultrastructural evaluation. Men ( n = 17) and women ( n = 19) completed 90-min cycling at 63% V̇o2peak. Biopsies were taken before and after exercise and fixed for electron microscopy to determine IMCL size, # IMCL/area, IMCL area density, and the % IMCL touching mitochondria. Women had a lower RER and carbohydrate oxidation rate and a higher lipid oxidation rate during exercise ( P < 0.05), compared with men. Women had a higher # IMCL/area and IMCL area density ( P < 0.05), compared with men. Women, but not men, had a higher % IMCL touching mitochondria postexercise ( P = 0.03). Exercise decreased IMCL area density ( P = 0.01), due to a decrease in the # IMCL/area ( P = 0.02). There was no sex difference in IMCL size or net use. In conclusion, women have higher IMCL area density compared with men, due to an increased # IMCL and not an increased IMCL size, as well as an increased % IMCL touching mitochondria postexercise. Endurance exercise resulted in a net decrease in IMCL density due to decreased number of IMCL, not decreased IMCL size, in both sexes.

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The use of experimental design methodology for investigating a lipid oxidation rate assay

Chemometrics and Intelligent Laboratory Systems ◽

10.1016/j.chemolab.2007.11.001 ◽

2008 ◽

Vol 91 (2) ◽

pp. 164-172

Author(s):

Martin Høy ◽

Revilija Mozuraityte ◽

Vegard Segtnan ◽

Ivar Storrø ◽

Bjørn Helge Mevik ◽

...

Keyword(s):

Experimental Design ◽

Lipid Oxidation ◽

Oxidation Rate ◽

Design Methodology ◽

Experimental Design Methodology

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Preliminary findings on the role of PLIN1 polymorphisms on body composition and energy metabolism response to energy restriction in obese women

British Journal Of Nutrition ◽

10.1017/s0007114511000432 ◽

2011 ◽

Vol 106 (4) ◽

pp. 486-490 ◽

Cited By ~ 18

Author(s):

J. R. Ruiz ◽

E. Larrarte ◽

J. Margareto ◽

R. Ares ◽

P. Alkorta ◽

...

Keyword(s):

Body Composition ◽

Energy Metabolism ◽

Waist Circumference ◽

Lipid Oxidation ◽

Body Fat ◽

Oxidation Rate ◽

Energy Restriction ◽

Obese Women ◽

Restricted Diet ◽

Induced Changes

The aim of the present study was to investigate the association of PLIN1 11482G>A (rs894160) and PLIN1 13041A>G (rs2304795) polymorphisms with body composition, energy and substrate metabolism, and the metabolic response to a 12-week energy-restricted diet in obese women. The study comprised a total of seventy-eight obese (BMI 34·0 (sd 2·8) kg/m2) women (age 36·7 (sd 7) years). We measured weight, height and waist circumference before and after a 12-week controlled energy-restricted diet intervention. Body fat mass and lean mass were measured by dual-energy X-ray absorptiometry. RMR and lipid oxidation rate were measured by indirect calorimetry. We also analysed fasting plasma glucose, insulin, cholesterol and leptin. Women carrying the 11482A allele had a lower reduction in waist circumference than non-A allele carriers (3·2 (sd 0·5) v. 4·6 (sd 0·6) %, respectively, P = 0·047; P for gene–diet interaction = 0·064). Moreover, women with the 11482A allele had a higher decrease in lipid oxidation rate than non-A allele carriers (58·9 (sd 6·7) v. 31·3 (sd 8·2) %, respectively, P = 0·012; P for gene–diet interaction = 0·004). There was no interaction effect between the 13041A>G polymorphism and diet-induced changes on the outcome variables (all P>0·1). These results confirm and extend previous findings suggesting that the PLIN1 11482G>A polymorphism plays a modulating role on diet-induced changes in body fat and energy metabolism in obese women.

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Glucose-induced thermogenesis, inhibition of lipid oxidation rate and autonomic dysfunction in non-diabetic obese women

International Journal of Obesity ◽

10.1038/sj.ijo.0800609 ◽

1998 ◽

Vol 22 (6) ◽

pp. 494-499 ◽

Cited By ~ 11

Author(s):

P Valensi ◽

B Lormeau ◽

M Dabbech ◽

P Miossec ◽

J Pariès ◽

...

Keyword(s):

Lipid Oxidation ◽

Autonomic Dysfunction ◽

Oxidation Rate ◽

Obese Women

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Peripheral blood mitochondrial DNA content correlates with lipid oxidation rate during euglycemic clamps in healthy young men

Diabetes Research and Clinical Practice ◽

10.1016/s0168-8227(99)00086-8 ◽

1999 ◽

Vol 46 (2) ◽

pp. 149-154 ◽

Cited By ~ 11

Author(s):

Kyong Soo Park ◽

Ji Hyun Song ◽

Ki-Up Lee ◽

Cheol Soo Choi ◽

Jae Joon Koh ◽

...

Keyword(s):

Mitochondrial Dna ◽

Lipid Oxidation ◽

Dna Content ◽

Peripheral Blood ◽

Oxidation Rate ◽

Young Men ◽

Mitochondrial Dna Content

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Elevated lipid oxidation is associated with exceeding gestational weight gain recommendations and increased neonatal anthropometrics: a cross-sectional analysis

BMC Pregnancy and Childbirth ◽

10.1186/s12884-021-04053-4 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Jill M. Maples ◽

Samantha F. Ehrlich ◽

Nikki B. Zite ◽

Kevin J. Pearson ◽

W. Todd Cade ◽

...

Keyword(s):

Weight Gain ◽

Lipid Oxidation ◽

Gestational Weight Gain ◽

Oxidation Rate ◽

Weight Status ◽

Fat Free Mass ◽

Neonatal Outcomes ◽

Cross Sectional ◽

Substrate Metabolism ◽

Gestational Weight

Abstract Background Deviations from gestational weight gain (GWG) recommendations are associated with unfavorable maternal and neonatal outcomes. There is a need to understand how maternal substrate metabolism, independent of weight status, may contribute to GWG and neonatal outcomes. The purpose of this study was to explore the potential link between maternal lipid oxidation rate, GWG, and neonatal anthropometric outcomes. Methods Women (N = 32) with a lean pre-pregnancy BMI were recruited during late pregnancy and substrate metabolism was assessed using indirect calorimetry, before and after consumption of a high-fat meal. GWG was categorized as follows: inadequate, adequate, or excess. Shortly after delivery (within 48 h), neonatal anthropometrics were obtained. Results Using ANOVA, we found that fasting maternal lipid oxidation rate (grams/minute) was higher (p = 0.003) among women with excess GWG (0.1019 ± 0.0416) compared to women without excess GWG (inadequate = 0.0586 ± 0.0273, adequate = 0.0569 ± 0.0238). Findings were similar when lipid oxidation was assessed post-meal and also when expressed relative to kilograms of fat free mass. Absolute GWG was positively correlated to absolute lipid oxidation expressed in grams/minute at baseline (r = 0.507, p = 0.003), 2 h post-meal (r = 0.531, p = 0.002), and 4 h post-meal (r = 0.546, p = 0.001). Fasting and post-meal lipid oxidation (grams/minute) were positively correlated to neonatal birthweight (fasting r = 0.426, p = 0.015; 2-hour r = 0.393, p = 0.026; 4-hour r = 0.540, p = 0.001) and also to neonatal absolute fat mass (fasting r = 0.493, p = 0.004; 2-hour r = 0.450, p = 0.010; 4-hour r = 0.552, p = 0.001). Conclusions A better understanding of the metabolic profile of women during pregnancy may be critical in truly understanding a woman’s risk of GWG outside the recommendations. GWG counseling during prenatal care may need to be tailored to women based not just on their weight status, but other metabolic characteristics.

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