scholarly journals Application of Amylolytic Lactobacillus fermentum 04BBA19 in Fermentation for Simultaneous Production of Thermostable Alpha-Amylase and Lactic Acid

Author(s):  
Bertrand Tatsinkou ◽  
Frederic Tave

2021 ◽  
Author(s):  
Xianrong Zhou ◽  
Guan-sheng Shang ◽  
Qian Tan ◽  
Qin He ◽  
Xiaoyu Tan ◽  
...  

With the increasing incidence of type 2 diabetes, it is imperative to identify how to effectively prevent or treat this disease. Studies have shown that some lactic acid bacteria can...



2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Sivasamy Gomathi ◽  
Ponnusamy Sasikumar ◽  
Kolandaswamy Anbazhagan ◽  
Sundaresan Sasikumar ◽  
Murugan Kavitha ◽  
...  

Lactic acid bacteria (LAB) have the potential to degrade intestinal oxalate and this is increasingly being studied as a promising probiotic solution to manage kidney stone disease. In this study, oxalate degrading LAB were isolated from human faeces and south Indian fermented foods, subsequently assessed for potential probiotic propertyin vitroandin vivo. Based on preliminary characteristics, 251 out of 673 bacterial isolates were identified as LAB. A total of 17 strains were found to degrade oxalate significantly between 40.38% and 62.90% and were subjected to acid and bile tolerance test. Among them, nine strains exhibited considerable tolerance up to pH 3.0 and at 0.3% bile. These were identified asLactobacillus fermentumandLactobacillus salivariususing 16S rDNA sequencing. Three strains,Lactobacillus fermentumTY5,Lactobacillus fermentumAB1, andLactobacillus salivariusAB11, exhibited good adhesion to HT-29 cells and strong antimicrobial activity. They also conferred resistance to kanamycin, rifampicin, and ampicillin, but were sensitive to chloramphenicol and erythromycin. The faecal recovery rate of these strains was observed as 15.16% (TY5), 6.71% (AB1), and 9.3% (AB11) which indicates the colonization ability. In conclusion, three efficient oxalate degrading LAB were identified and their safety assessments suggest that they may serve as good probiotic candidates for preventing hyperoxaluria.



2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Junxiao Zhang ◽  
Ling Chen ◽  
Lingyan Zhang ◽  
Qiuping Chen ◽  
Fang Tan ◽  
...  

Yak yogurt is mainly produced in Qinghai-Tibet Plateau. It is a kind of naturally fermented dairy product. It contains abundant microorganisms. Lactobacillus fermentum (LF) HFY03 is a lactic acid bacteria derived from it. Our main research content is to study the influence of LF-HFY03 on the antifatigue and antioxidation ability of running exhausted mice. We gave different doses of LF-HFY03 to mice by gavage for 4 weeks. We selected vitamin C as the positive control group, mainly to study the relationship between antioxidant capacity and fatigue resistance and LF-HFY03 in mice with running exhaustion. The results showed that LF-HFY03 and vitamin C could significantly improve the running time of mice. And with the increase in LF-HFY03 concentration, the exhaustion time of mice was also extended. LF-HFY03 can reduce the content of urea nitrogen and lactic acid and also can increase the content of free fatty acids and liver glycogen. The levels of alanine aminotransferase, serum creatine kinase, and aspartate aminotransferase in mice decreased gradually as the antioxidant peptide level of walnut albumin increased. LF-HFY03 can reduce malondialdehyde (MDA) levels in a quantification-dependent manner and can also increase catalase (CAT) and superoxide dismutase (SOD) levels. LF-HFY03 can also increase the expressions of CAT mRNA, Cu/Zn-SOD, and Mn-SOD in the liver of mice. At the same time, LF-HFY03 can also increase the expression of protein of threonine transporter 1 (AST1)/alanine/cysteine/serine, mRNA, nNOS, and eNOS. At the same time, the solution could reduce the expression of TNF-α, syncytin-1, and inducible nitric oxide synthase (iNOS). The results showed that LF-HFY03 has a high development and application prospect as an antifatigue probiotic nutritional supplement.



2019 ◽  
pp. 70-72
Author(s):  
A. О. Karapetyan

Aim of the study: To present an analysis of the literature data on the role of Lactobacillus fermentum CECT5716 in the prevention and treatment of postpartum lactation complications. Materials and methods: literature sources published in the Pubmed, Scopus database were searched. Results: according to randomized clinical studies, the use of Lactobacillus fermentum CECT5716 in the postnatal period by mothers leads to the correction of breast microbiota by reducing the number of pathogenic bacteria and increasing lactic-acid bacteria, reducing the content of inflammatory factors in breast milk. Conclusion: Lactobacillus fermentum CECT5716 is an effective and safe way to prevent and treat postpartum lactation complications.



2012 ◽  
Vol 50 (No. 9) ◽  
pp. 415-420 ◽  
Author(s):  
V. Strompfova ◽  
M. Marcinakova ◽  
S. Gancarcikova ◽  
Z. Jonecova ◽  
L. Scirankova ◽  
...  

Probiotics have been used with increasing frequency in nutrition and for prophylactic purposes during the last years. In the present study we investigated the effect of Lactobacillus fermentum AD1 – canine isolate on selected intestinal microbial groups, weight gain, organic acids, haematology, glutathione peroxidase and phagocytosis of leucocytes in 2-days-old Japanese quail (Coturnix coturnix japonica). The results demonstrated that the 4-day application of this strain significantly increased the population of lactic acid bacteria – lactobacilli and enterococci in faeces (P < 0.01 and/or P < 0.001) and caecum of quail (P < 0.001) and significantly decreased the counts of E. coli in faeces (P < 0.05). The daily weight gain was increased by 14%. Although intestinal pH of both groups of birds was similar, the concentration of lactic acid was significantly increased in the experimental group (P < 0.05). The concentration of other organic acids (acetic, acetoacetic, formic, succinic, valeric, propionic, butyric) as well as blood glutathione peroxidase was not influenced. The index of phagocytic activity of leucocytes was significantly improved (P < 0.01).





Author(s):  
Mita Ekamelinda ◽  
I Wayan Suardana ◽  
Komang Januartha Putra Pinatih

Lactic acid bacteria are the type of bacteria that has benefits in food and health industries as a biopreservative, fermentative, or probiotics. Bali cattle are known as potential host for specific lactic acid bacteria. The aim of this study is to identify phenotypically lactic acid bacteria 9A isolated from bali cattle’s colon, that producing a substance which known has potency as antimicrobial. In this study, phenotypic identification included conventional method and API 50 CHL. The result of this study showed that lactic acid bacteria isolate 9A was Streptococcus sp., whereas identification by kit API 50 CHL showed isolate 9A as Lactobacillus fermentum with 83% identity. The difference between the results of conventional method and kit API 50 CHL, may indicate the difference in sensitivity and specificity of the two methods, hence it needs further confirmation.



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