scholarly journals Lactobacillus fermentum CECT5716 – prevention and treatment of lactational mastitis

2019 ◽  
pp. 70-72
Author(s):  
A. О. Karapetyan
Keyword(s):  
Lactic Acid ◽  
Breast Milk ◽  
Pathogenic Bacteria ◽  
Postnatal Period ◽  
Lactobacillus Fermentum ◽  
Inflammatory Factors ◽  
Prevention And Treatment ◽  
Lactational Mastitis ◽  
Scopus Database

Aim of the study: To present an analysis of the literature data on the role of Lactobacillus fermentum CECT5716 in the prevention and treatment of postpartum lactation complications. Materials and methods: literature sources published in the Pubmed, Scopus database were searched. Results: according to randomized clinical studies, the use of Lactobacillus fermentum CECT5716 in the postnatal period by mothers leads to the correction of breast microbiota by reducing the number of pathogenic bacteria and increasing lactic-acid bacteria, reducing the content of inflammatory factors in breast milk. Conclusion: Lactobacillus fermentum CECT5716 is an effective and safe way to prevent and treat postpartum lactation complications.

scholarly journals Effect of breast milk microbiome on the health of mothers and newborns

2019 ◽  
pp. 36-40
Author(s):  
О. A. Pustotina ◽  
A. A. Seliverstov
Keyword(s):  
Lactic Acid ◽  
Mammary Gland ◽  
Breast Milk ◽  
Lactic Acid Bacteria ◽  
Newborn Health ◽  
Risk Groups ◽  
Maternal And Newborn Health ◽  
Microbial Composition ◽  
Lactational Mastitis ◽  
Maternal And Newborn

The article presents updated data on the microbial composition of breast milk in healthy women and women with lactational mastitis. The authors describe the main ways of the formation of mammary gland microbiome and its effect on the maternal and newborn health. The feasibility of using probiotic agents created on the basis of strains of lactic acid bacteria isolated from breast milk is substantiated both for the treatment of lactational mastitis and for its prevention in risk groups.

scholarly journals Lactic Acid Bacteria Biosurfactant Role That Isolated from Human Breast Milk in Inhibit Eyes Pathogenic Bacteria

2018 ◽  
Vol 31 (2) ◽  
pp. 31
Author(s):  
Yusra M.B. Muhsin ◽  
Huda Z. Majeed ◽  
Basam Basim Mohammed ◽  
Salih A.A. Mohammed
Keyword(s):  
Lactic Acid ◽  
Breast Milk ◽  
Lactic Acid Bacteria ◽  
Surface Activity ◽  
Pathogenic Bacteria ◽  
Good Effect ◽  
Human Breast Milk ◽  
Human Breast ◽  
Wide Range ◽  
Normal Method

     Biosurfactants have a wide-range of applications due to their unique properties like specificity, not toxicity (from LAB) and relative ease of preparation. These properties hold promise of biosurfactants to increase breast milk benefit were isolated and described into Lactobacillus plantarum, Lactobacillus fermentum ,Lactococcuslactis, and Leuconostocmesenteroides.The degree of microbial destruction of disease, which promotes  the effective remediation of disease spreading.This paper presents a review of available research, methods and publications regarding Biosurfactant extraction from Lactic Acid bacteria isolated from human breast milk. 3 samples of human breast milk was provided, LAB were isolated and described, Biosurfactants recovery and surface activity were tested and extracted endo and extra cellular. In other side 26 samples from eye patients were ordered, diagnosed and their sensitivity to biosurfactant were studied. The results showed that 5 isolates of LAB from human breast milk were biosurfactant producer but L. plantarum was the more efficiency in surface activity. In other side, out of 26 eyes sample 18 were positive to pathogenic bacteria included E.coli (7), Klebseilla (5), Staphylococcus aureus(3)and S.epidermides (3).Extracellular Biosurfactanthad good effect against tested bacteria, but endocellular (extraction by normal method) had not any effect against any bacteria, whereas by solvents method were the more active against all tested bacteria. The results are promising enough to continue the quest for enhancement of inhibition growth of pathogenic bacteria with biosurfactant application (extracted extracellular by solvents) to look forward for biosurfactant as a solution of antibiotic resistance problem. In this study we concluded thatL. plantarum was the more effectiveness in biosurfactant surface activity and the extracellular biosurfactant by solvent method for extraction were better than endocellular and normal method of extraction.

scholarly journals Effect of Lactobacillus sp. isolates supernatant on Escherichia coli O157:H7 enhances the role of organic acids production as a factor for pathogen control

2015 ◽  
Vol 35 (4) ◽  
pp. 353-359 ◽  
Author(s):  
Larissa B. Poppi ◽  
Javier D. Rivaldi ◽  
Thais S. Coutinho ◽  
Claudete S. Astolfi-Ferreira ◽  
Antonio J. Piantino Ferreira ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Foodborne Pathogens ◽  
Pathogenic Bacteria ◽  
Inhibitory Effect ◽  
Antagonistic Effect ◽  
Pure Cultures ◽  
Pathogen Control ◽  
The Individual

Many attempts have been made to establish the control of foodborne pathogens through Lactobacillus isolates and their metabolism products with success being obtained in several situations. The aim of this study was to investigate the antagonistic effect of eight Lactobacillusisolates, including L. caseisubsp. pseudoplantarum,L. plantarum, L. reuteri and L. delbrueckii subsp. delbrueckii, on the pathogenic Escherichia colistrain O157:H7. The inhibitory effect of pure cultures and two pooled cultures supernatants of Lactobacillus on the growth of pathogenic bacteria was evaluated by the spot agar method and by monitoring turbidity. Antimicrobial activity was confirmed for L. reuteri and L. delbrueckii subsp. delbrueckii and for a pool of lactic acid bacteria. The neutralized supernatant of the pool exerted a higher antimicrobial activity than that of the individual strains. Furthermore, D-lactic acid and acetic acid were produced during growth of the Lactobacillus isolates studied.

scholarly journals Prevention and treatment of lactostasis and (or) mastitis in the pediatric area: practical aspects

2021 ◽  
pp. 18-23
Author(s):  
A. I. Safina
Keyword(s):  
Drug Therapy ◽  
Breast Milk ◽  
Clinical Case ◽  
Left Breast ◽  
Lactobacillus Fermentum ◽  
Health State ◽  
Continuous Administration ◽  
Once Daily ◽  
Prevention And Treatment ◽  
Breast Lumps

Introduction. Incomplete breast emptying is the main cause of lactostasis, which can contribute to the development of mastitis. The article discusses the issues of prevention and treatment of lactostasis in breastfeeding mothers in the district covered by childhealth care: how to arrange breastfeeding, expression of breast milk, regime issues from the baby’s first days, as well as present-day knowledge on breast milk microbiota dysbiosis and an option of probiotic therapy. Clinical cases are presented.Clinical case 1. A breastfeeding mother found breast lumps on the one side 2 weeks after her labour. Lactostasis was diagnosed, it was caused by inappropriate breastfeeding technique. The breastfeeding mother was provided with the recommendations for proper performance of breastfeeding technique and prescribed the drug therapy: ibuprofen 400 mg once at fever ≥ 38.5 ºC and Lactobacillus fermentum CEST5716 – 1 capsule once daily. On the 3rd day, her health state significantly improved, the pain reduced, the temperature returned to normal, the breast lumps practically disappeared, on the 5th day the pain and breast lumps completely disappeared.Clinical case 2. A breastfeeding mother, first labour, 2 months have passed since her labour, exclusively breastfeeding on demand, complaints of pain in the left breast, local hyperemia 3 x 3 cm occurred, no breast lumps, local pain elicited by palpation, fever of 37.2 ºC. The breastfeeding mother was diagnosed with lactostasis, she was provided with recommendations for the arrangement of breastfeeding and expression of breast milk; not proscribed with antibacterial therapy, but the therapy with Lactobacillus fermentum CEST5716, 1 capsule once daily for 1 month. A day later, her health state improved: the pain reduced, the hyperemia disappeared, her body temperature returned to normal. The patient was recommended non-drug measures for preventing lactostasis and continuous administration of Lactobacillus fermentum CEST5716 – 1 capsule daily for 1 month.Clinical case 3. A breastfeeding mother detected daily lactostasis in her right breast 3 months after her labour. It might had been caused by chest contusion before the onset of the 1st episode of lactostasis. Medical examination revealed calcifications in her breast duct. Against the background of non-drug therapy, the patient adminstered Lactobacillus fermentum CEST5716 – 1 capsule daily, which resulted in a positive effect in a week’s time. The follow-up observation showed that lactostasis completely resolved without relapses in a 1.5-months’ time.Сonclusion. The correct breastfeeding technique is key to the prevention of lactostasis and mastitis, and the use of probiotic therapy Lactobacillus fermentum CEST5716 is an effective and safe drug treatment.

scholarly journals The Protective Effects of Lactobacillus plantarum KLDS 1.0344 on LPS-Induced Mastitis In Vitro and In Vivo

2021 ◽  
Vol 12 ◽  
Author(s):  
Qingxue Chen ◽  
Song Wang ◽  
Jiayao Guo ◽  
Qinggang Xie ◽  
Smith Etareri Evivie ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Pathogenic Bacteria ◽  
Mammary Epithelial ◽  
Inflammatory Factors ◽  
Protective Effects ◽  
E Coli ◽  
Tnf Α

Cow mastitis, which significantly lowers milk quality, is mainly caused by pathogenic bacteria such as E. coli. Previous studies have suggested that lactic acid bacteria can have antagonistic effects on pathogenic bacteria that cause mastitis. In the current study, we evaluated the in vitro and in vivo alleviative effects of L. plantarum KLDS 1.0344 in mastitis treatment. In vitro antibacterial experiments were performed using bovine mammary epithelial cell (bMEC), followed by in vivo studies involving mastitis mouse models. In vitro results indicate that lactic acid was the primary substance inhibiting the E. coli pathogen. Meanwhile, treatment with L. plantarum KLDS 1.0344 can reduce cytokines’ mRNA expression levels in the inflammatory response of bMEC induced by LPS. In vivo, the use of this strain reduced the secretion of inflammatory factors IL-6, IL-1β, and TNF-α, and decreased the activity of myeloperoxidase (MPO), and inhibited the secretion of p-p65 and p-IκBα. These results indicate that L. plantarum KLDS 1.0344 pretreatment can reduce the expression of inflammatory factors by inhibiting the activation of NF-κB signaling pathway, thus exerting prevent the occurrence of inflammation in vivo. Our findings show that L. plantarum KLDS 1.0344 has excellent properties as an alternative to antibiotics and can be developed into lactic acid bacteria preparation to prevent mastitis disease.

Von Willebrand disease and Weibel-Palade bodies

2010 ◽  
Vol 30 (03) ◽  
pp. 150-155 ◽  
Author(s):  
J. W. Wang ◽  
J. Eikenboom
Keyword(s):  
Platelet Adhesion ◽  
Coagulation Factor ◽  
Von Willebrand Disease ◽  
Inflammatory Factors ◽  
Limited Data ◽  
Storage Organelle ◽  
And Function ◽  
Von Willebrand ◽  
Willebrand Factor

SummaryVon Willebrand factor (VWF) is a pivotal haemostatic protein mediating platelet adhesion to injured endothelium and carrying coagulation factor VIII (FVIII) in the circulation to protect it from premature clearance. Apart from the roles in haemostasis, VWF drives the formation of the endothelial cell specific Weibel-Palade bodies (WPBs), which serve as a regulated storage of VWF and other thrombotic and inflammatory factors. Defects in VWF could lead to the bleeding disorder von Willebrand disease (VWD).Extensive studies have shown that several mutations identified in VWD patients cause an intracellular retention of VWF. However, the effects of such mutations on the formation and function of its storage organelle are largely unknown. This review gives an overview on the role of VWF in WPB biogenesis and summarizes the limited data on the WPBs formed by VWD-causing mutant VWF.

Isolation and identification of lactic acid bacteria in Bakasang as fermented microbe starter

2013 ◽  
pp. 48
Author(s):  
J Aquarista Ingratubun ◽  
Frans G Ijong ◽  
Hens Onibala
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Pathogenic Bacteria ◽  
Raw Materials ◽  
Fermented Food ◽  
Plate Count ◽  
Local Market ◽  
Bacillus Sp ◽  
Isolation And Identification ◽  
Total Plate Count

Food fermentation is one of various food processing techniques that has sufficient benefits of nutrition values, and also contains lactic acid bacteria which potentially inhibit pathogenic bacteria, thus prolong shelf life of  products. Bakasang is a traditional fermented food from North Sulawesi since many years ago. Reported research of bakasang previously had described that lactic acid bacteria was the dominant isolates and therefore current research  aimed to isolate and identify the lactic acid bacteria which associated during fermentation day 1 and day 15, respectively. Raw materials used were 5 kg intestine and liver of skipjack brought from local market Bersehati Manado. The intestine and liver of skipjack were washed and smashed and mixed with 10% salt  and 5% rice  from weight of the samples and then filled into bottle to be fermented for 15 days. Every 3 days (1,3,6,9,12,15), the samples were collected and analyzed for total lactic acid bacteria by using Total Plate Count Method on de Mann Rogosa Sharpe Agar after incubation at 37°C for 24 h. The colonies  grown were transferred to Tryptic Soy Broth and followed by streaking them on Tryptic Soy Agar and the free growing colony on agar medium were isolated into slant agar which were used for biochemical test such as Gram’s staining, motility test, catalase test, oksidase test, H2S test, IMVIC test (Indole, Methyl Red, Voges Proskauer, Citrate) and carbohydrate fermentation. The results showed that Lactobacillus sp., Bacillus sp., Eubacterium sp., and Bifidobacterium sp. All these four bacteria were distributed from day 1 to day 15 of the fermentation process© Fermentasi bahan pangan merupakan salah satu dari sekian banyak teknik pengolahan makanan yang mempunyai banyak manfaat dari kualitas gizi, mengandung bakteri asam laktat sehingga menghambat bakteri patogen sehingga daya simpan lebih panjang. Bakasang merupakan makanan fermentasi tradisional masyarakat Sulawesi Utara yang sudah ada sejak lama. Penelitian yang telah dilakukan terhadap bakasang menghasilkan informasi bahwa terdapat bakteri asam laktat pada bakasang sehingga menjadi tujuan untuk mengisolasi dan identifikasi bakteri asam laktat selama proses fermentasi 1-15 hari. Bahan baku bakasang ialah jeroan (usus dan hati) ikan cakalang Katsuwonis pelamis sebanyak 5 kg yang diambil dari pasar Bersehati Manado. Sampel jeroan dibersihkan kemudian dihancurkan, ditambahkan garam 10% dan nasi 5% kemudian difermentasi selama 15 hari dengan mengambil tiap-tiap sampel setiap 1, 3, 6, 9, 12, dan 15 untuk dihitung jumlah bakteri asam laktat dengan menggunakkan metode Total Plate Count pada media de Mann Rogosa Sharpe Agar dan koloni yang tumbuh di tumbuhkan  kembali pada media Tryptic Soy Broth  dan digores kembali pada media Tryptic Soy Agar, koloni yang tumbuh digores pada media slant agar yang selanjutnya diidentifikasi bakteri asam laktat berdasarkan uji biokimia yaitu uji pewarnaan Gram, uji motility, uji katalase, uji oksidase, uji H2S dan uji IMVIC (Indole, MethylRed, Voges Proskauer, Citrate). Hasil menunjukkan bahwa selama proses fermentasi berlangsung terdapat 4 genera bakteri asam laktat sesuai yaitu Lactobacillus sp., Bacillus sp., Eubacterium sp., dan Bifidobacterium sp., ke 4 genera ini tersebar pada fermentasi hari 1 sampai hari ke 15©

Bioenhancing Effect of Cow Urine Distillate and Pepper Extract on Antibacterial Activity of Azadirachta Indica Leaves

1970 ◽  
Vol 9 (2) ◽  
pp. 3212-3214
Author(s):  
Pramod Dhakal ◽  
Ankit a Achary ◽  
Vedamurthy Joshi
Keyword(s):  
Antibacterial Activity ◽  
Azadirachta Indica ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Watery Diarrhea ◽  
Diffusion Method ◽  
E Coli ◽  
Drug Molecules ◽  
Cow Urine

Bioenhancers are drug facilitator which do not show the typical drug activity but in combination to enhance the activity of other molecule in several way including increase the bioavailability of drug across the membrane, potentiating the drug molecules by conformational interaction, acting as receptor for drug molecules and making target cell more receptive to drugs and promote and increase the bioactivity or bioavailability or the uptake of drugs in combination therapy. The objective of the present study was to evaluate the antibacterial and activity of combination in Azadirachta indica extract with cow urine distillate and pepper extract against common pathogenic bacteria, a causative agent of watery diarrhea. It has been found that Indian indigenous cow urine and its distillate also possess bioenhancing ability. Bioenhancing role of cow urine distillate (CUD) and pepper extract was investigated on antibacterial activity of ethanol extract of Azadirachta indica. Antibacterial activity of ethanol extract neem alone and in combination with CUD and pepper extract were determined the ATCC strains against Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and E-coli by cup plate diffusion method. Ethanol extract of neem has showed more effect on P. aeruginosa, E-coli than S. aureus and K. pneumonia with combination of CUD and pepper extract. CUD and pepper did not show any inhibition of test bacteria in low concentration. The antibacterial effect of combination of extract and CUD was higher than the inhibition caused by extract alone and is suggestive of the bioenhancing role of cow urine distillate and pepper. Moreover, inhibition of test bacteria was observed with less concentration of extract on combining with CUD

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