scholarly journals Characteristics of Intestinal Flora in Pregnant Women with Mild Thalassemia Revealed by Metagenomics

2021 ◽  
Vol 14 (11) ◽  
Author(s):  
Yong-Zhi Lun ◽  
Wei Qiu ◽  
Wenqi Zhao ◽  
Hua Lin ◽  
Mintao Zhong ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Pathogenic Bacteria ◽  
Sequence Data ◽  
Intestinal Flora ◽  
Control Group ◽  
Metagenomic Sequencing ◽  
Sequencing Technology ◽  
Illumina Hiseq ◽  
Multiple Comparison Test ◽  
Wilcoxon Rank Sum Test

Background: At present, there is no report that the intestinal flora of pregnant women with mild thalassemia is different from that of healthy pregnant women. Objectives: This study compared the composition and changes of the intestinal flora of pregnant women with mild thalassemia to those of healthy pregnant women using metagenomic sequencing technology and evaluated the potential microecological risk for pregnant women and the fetus. Methods: The present study was carried out on 14 mild thalassemia pregnant women with similar backgrounds in the Affiliated Hospital of Putian University, Fujian, China. In the same period, 6 healthy pregnant women were selected as the control group. The genomic deoxyribonucleic acid was extracted from the sable stool samples of pregnant women. Illumina HiSeq sequencing technology was adopted after library preparation. Prodigal software (ver 2.6.3, Salmon software (ver 1.6.0, and Kraken software (ver 2) were used to analyze the sequence data. Moreover, analysis of variance and Duncan’s multiple-comparison test or Wilcoxon rank-sum test were used as statistical methods. Results: The characteristics of the intestinal flora of pregnant women with mild thalassemia differed significantly from those of healthy pregnant women, showing an increase in some conditionally pathogenic bacteria (e.g., Prevotella stercorea rose and Escherichia coli) and a decrease in some probiotic bacteria, which might affect pregnant women and cause physiological function damage to their offspring by changing metabolic pathways; however, further validation is needed. Conclusions: The diversity and composition of intestinal flora in pregnant women with mild thalassemia vary significantly from those in healthy pregnant women, especially at the genus and species levels, representing more profound alterations in intestinal microecology.

scholarly journals Effect of Gut Microbiotas Diversity During 32-39 Weeks of Gestation on Postpartum Depression

2020 ◽  
Author(s):  
Xiaoyan Zhang ◽  
Qiangsheng Gan ◽  
Hongling Yang ◽  
Weitao Ye ◽  
Xueqin Zhao ◽  
...  
Keyword(s):  
Postpartum Depression ◽  
Gut Microbiota ◽  
Pregnant Women ◽  
Pathogenic Bacteria ◽  
Intestinal Flora ◽  
Control Group ◽  
Control Groups ◽  
Mainland Chinese ◽  
Pregnancy And Postpartum ◽  
And Control

Abstract Background: Patients with major depression are accompanied by intestinal flora flocculation; however, the relationship between the composition of gut microbiota in pregnancy and postpartum depression (PPD) has not been established. In this study we determined the effect of the gut microbiota in pregnant women during 32-39 weeks of gestation on PPD.Methods: Participants (n = 74) were enrolled between 2016–2017 from the Guangzhou Women and Children’s Medical Centre (GWCMC). Stool samples were collected during 32-39 weeks of gestation, and the relative abundance of fecal microbiota was characterized by 16S rRNA sequencing. The parturients completed the mainland Chinese version of the Edinburgh Postnatal Depression Scale (EPDS) 42 days postpartum to detect PPD. The linear discriminant analysis (LDA) effect size (LEfSe) method was used to identify bacterial population differences between the PPD and control groups.Results: The top three bacteria phyla in the PPD and control groups were Firmicutes, Bacteroidetes, and Actinobacteria. Compared with healthy pregnant women, the alpha diversity index of the PPD group was lower. Beta diversity analysis was performed by PCoA showing that no significant differences in bacterial community structures between the two groups (R2 = 0.013, P = 0.549). The composition of gut microbiota during 32-39 weeks of gestation of the two groups was different. At the genera level, Acinetobacter, Plesiomonas, Enterococcus, Olsenella, Alloscardovia, and Anaerotruncus were increased in the PPD group, while Lactococcus, Adlercreutzia, Clostridium, Coprococcus, and unclassified-Clostridiales were decreased. At the species level, hypermegale, uli, casseliflavus, and hathewayi were increased in the PPD group, and celatum was increased in the control group.Conclusions: During 32-39 weeks of gestation, a reduction in diversity of gut microbiota and anti-inflammatory bacteria, and an increase in opportunistic pathogenic bacteria are more likely to cause PPD.

scholarly journals Effect of Feed Supplemented With Vitamin C on Microbial Flora of Swine Faeces and Blood Morphology

2012 ◽  
Vol 56 (2) ◽  
pp. 171-175
Author(s):  
Beata Trawińska ◽  
Jerzy Lechowski ◽  
Antoni Polonis ◽  
Marta Kowaleczko
Keyword(s):  
Vitamin C ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Intestinal Flora ◽  
Bacterial Count ◽  
Control Group ◽  
Total Bacterial Count ◽  
E Coli ◽  
Bacteriological Evaluation ◽  
Experimental Group

Abstract The studies involved gilts 6 weeks pre-partum allocated into the control and experimental groups. The experimental group received vitamin C at the dose of 2.5g/100 kg b.w./day. Faeces for analyses were collected for 3- and 6- week period of vitamin C dietary inclusion. The faecal material underwent quantitative and qualitative bacteriological evaluation. The count of Enterobacteriaceae was evaluated. The qualitative examination of these bacteria was made using the micro-plate method API 20E. Feed supplemented with vitamin C contributed to a statistically significant decline of the total bacterial count of Enterobacteriaceae in faeces. The qualitative bacterial analyses showed the presence of E. coli, Providecia sp., Proteus sp., and Salmonella sp. in the control and experimental groups. Enterobacter sp. was recovered only in the control group, while Lactobacillus sp. in the experimental group. The dietary administration of vitamin C significantly decreased the number of the studied bacterial species, except Salmonella rods. No statistically significant differences in the number of most blood morphotic elements following the 6- week supply of vitamin C were found; only the granulocyte count significantly increased, while lymphocyte numbers declined. Ascorbic acid inhibited the growth of pathogenic intestinal flora and reduced the pathogenic and relatively pathogenic bacteria count in the gastrointestinal tract and notably contributed to enhanced growth of beneficial bacteria.

scholarly journals Ginger Alleviates DSS-Induced Ulcerative Colitis Severity by Improving the Diversity and Function of Gut Microbiota

2021 ◽  
Vol 12 ◽  
Author(s):  
Shanshan Guo ◽  
Wenye Geng ◽  
Shan Chen ◽  
Li Wang ◽  
Xuli Rong ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Gut Microbiota ◽  
Pathogenic Bacteria ◽  
Intestinal Flora ◽  
Experimental Models ◽  
Intestinal Microbiome ◽  
Control Group ◽  
Therapeutic Effects ◽  
Sequencing Analysis ◽  
And Function

The effects of ginger on gastrointestinal disorders such as ulcerative colitis have been widely investigated using experimental models; however, the mechanisms underlying its therapeutic actions are still unknown. In this study, we investigated the correlation between the therapeutic effects of ginger and the regulation of the gut microbiota. We used dextran sulfate sodium (DSS) to induce colitis and found that ginger alleviated colitis-associated pathological changes and decreased the mRNA expression levels of interleukin-6 and inducible nitric oxide synthase in mice. 16s rRNA sequencing analysis of the feces samples showed that mice with colitis had an intestinal flora imbalance with lower species diversity and richness. At the phylum level, a higher abundance of pathogenic bacteria, Proteobacteria and firmicutes, were observed; at the genus level, most samples in the model group showed an increase in Lachnospiraceae_NK4A136_group. The overall analysis illustrated an increase in the relative abundance of Lactobacillus_murinus, Lachnospiraceae_bacterium_615, and Ruminiclostridium_sp._KB18. These increased pathogenic bacteria in model mice were decreased when treated with ginger. DSS-treated mice showed a lower abundance of Muribaculaceae, and ginger corrected this disorder. The bacterial community structure of the ginger group analyzed with Alpha and Beta indices was similar to that of the control group. The results also illustrated that altered intestinal microbiomes affected physiological functions and adjusted key metabolic pathways in mice. In conclusion, this research presented that ginger reduced DSS-induced colitis severity and positively regulated the intestinal microbiome. Based on the series of data in this study, we hypothesize that ginger can improve diseases by restoring the diversity and functions of the gut microbiota.

scholarly journals Tianwang Buxin Granules Influence the Intestinal Flora in Perimenopausal Insomnia

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Xiqian Yang ◽  
Hesong Xiao ◽  
Yi Zeng ◽  
Liangliang Huang ◽  
Ke Ji ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Controlled Trial ◽  
Intestinal Flora ◽  
World Health ◽  
Control Group ◽  
Metagenomic Sequencing ◽  
Clinical Trial Registry ◽  
Clinical Trial Registration ◽  
Faecalibacterium Prausnitzii ◽  
Health Organization

Study Objectives. To study the relationship between perimenopausal insomnia (PI) and intestinal flora and the potential mechanism of Tianwang Buxin granules (TWBXG) in exerting its clinical efficacy. Methods. The subjects included 13 PI patients from the Hubei Provincial Hospital of TCM, Hubei University of TCM, and Wuhan Traditional Chinese Medicine Hospital, and the corresponding noninsomniac spouses of the patients were selected as controls. TWBXG was continuously administered for 4 weeks. The feces of PI patients and their noninsomniac spouses before and after treatment with TWBXG were collected. The intestinal flora composition of each group was detected by metagenomic sequencing, and the efficacy of TWBXG was evaluated by the PSQI scale. Results. Compared with the control group, the model group showed an increase in the abundance of Roseburia faecis, Ruminococcus, Prevotella copri, Fusicatenibacter saccharivorans, and Blautia obeum, while those of Bacteroides, fecal Bacteroidetes, and Faecalibacterium prausnitzii were decreased. Compared with pretreatment, the PSQI score was significantly reduced ( P < 0.05 ), the abundance of Bacteroides, fecal Bacteroidetes, and Faecalibacterium prausnitzii increased, and that of Roseburia faecis, Ruminococcus, Prevotella copri, Fusicatenibacter saccharivorans, and Blautia obeum decreased after treatment. However, there was still a certain gap in the abundance of related flora in the treatment group compared with the control. Conclusion. PI is associated with disturbances in the intestinal flora and is mainly related to the disorders of Roseburia faecis, Ruminococcus, Prevotella copri, Fusicatenibacter saccharivorans, Blautia obeum, Bacteroides, fecal Bacteroidetes, and Faecalibacterium prausnitzii. TWBXG can effectively treat PI, and its effect may be achieved by regulating the disordered intestinal flora. Clinical Trials. The study was registered in the Chinese clinical trial registry and approved by the World Health Organization clinical trial registration platform (Effects of the modified Tianwang Buxin granule and modified Tianwang Buxin decoction pieces on insomnia: a randomized, controlled trial, ChiCTR-IPR-17011549).

scholarly journals Harnessing the strategy of metagenomics for exploring the intestinal microecology of sable (Martes zibellina), the national first-level protected animal

2020 ◽  
Author(s):  
Jiakuo Yan ◽  
Xiaoyang Wu ◽  
Jun Chen ◽  
Yao Chen ◽  
Honghai Zhang
Keyword(s):  
Information Processing ◽  
Complex Structure ◽  
Intestinal Flora ◽  
Metagenomic Library ◽  
Metagenomic Data ◽  
Metagenomic Sequencing ◽  
Sequencing Data ◽  
Illumina Hiseq ◽  
Martes Zibellina ◽  
Gene Functions

Abstract Sable (Martes zibellina), a member of family Mustelidae, order Carnivora, is primarily distributed in the cold northern zone of Eurasia. The purpose of this study was to explore the intestinal flora of the sable by metagenomic library-based techniques. Libraries were sequenced on an Illumina HiSeq 4000 instrument. The effective sequencing data of each sample was above 6,000 M, and the ratio of clean reads to raw reads was over 98%. The total ORF length was approximately 603,031, equivalent to 347.36 Mbp. We investigated gene functions with the KEGG database and identified 7,140 KEGG ortholog (KO) groups comprising 129,788 genes across all of the samples. We selected a subset of genes with the highest abundances to construct cluster heat maps. From the results of the KEGG metabolic pathway annotations, we acquired information on gene functions, as represented by the categories of metabolism, environmental information processing, genetic information processing, cellular processes and organismal systems. We then investigated gene function with the CAZy database and identified functional carbohydrate hydrolases corresponding to genes in the intestinal microorganisms of sable. This finding is consistent with the fact that the sable is adapted to cold environments and requires a large amount of energy to maintain its metabolic activity. We also investigated gene functions with the eggNOG database; the main functions of genes included gene duplication, recombination and repair, transport and metabolism of amino acids, and transport and metabolism of carbohydrates. In this study, we attempted to identify the complex structure of the microbial population of sable based on metagenomic sequencing methods, which use whole metagenomic data, and to map the obtained sequences to known genes or pathways in existing databases, such as CAZy, KEGG, and eggNOG. We then explored the genetic composition and functional diversity of the microbial community based on the mapped functional categories.

Learning of the biotope of maternity pathways of pregnant women with olygohydramnios

2018 ◽  
pp. 81-83
Author(s):  
I. Basiuha ◽  
Keyword(s):  
Pregnant Women ◽  
Genital Herpes ◽  
Pathogenic Bacteria ◽  
Enteric Bacteria ◽  
Main Group ◽  
Gardnerella Vaginalis ◽  
Control Group ◽  
Amniotic Fluid Index ◽  
Urogenital Infections

The article focuses on clinical-statistical analyses of microbiocenosis of maternity pathways of women with oligohydramnios, which will enable the usage of etiotropic mediator aimed at reducing of the frequency of fetus and newborn’s infecting. The objective: to learn the biotope of vaginal environment in the process of pregnancy complicated by oligohydramnios. Materials and methods. There were observed 120 pregnant women with oligohydramnios at gestation term of 27–29 weeks (the main group). 30 women with physiological pregnancy process constituted the control group. Oligohydramnios was diagnosed on the basis of complex examination of pregnant women at the term of 27–29 weeks with the help of standard determination of amniotic fluid index. All the women underwent bacterioscopic and bacteriological examination and detection of antigens to: chlamydia, ureaplasma, mycoplasma, virus of genital herpes, cytomegalic virus. Results. In the main group of women there was diagnosed ureaplasmosis, Gardnerella vaginalis, chlamydiosis, trichomoniasis, Candida albicans, more than half of women had increased concentration of pathogenic bacteria (staphylococcus epidermidis, aureus, saprophyticus, enteric bacteria, enterococcus faecalis), genital herpes, cytomegalovirus. Most of the representatives of the main group had bacterial infection of genital tract (69.17%), and complex bacterial-viruses infection (30.83%). Conclusion. Results of the research show that pregnant women with urogenital infections, inflammations of pelvic organs complicated by obstetrical – gynecological anamnesis should be referred to the group of high risk in reference to the development of oligohydramnios. Key words: pregnancy, microbiocenosis of vulva, oligohydramnios, infections.

scholarly journals Comparison of the Vitreous Fluid Bacterial Microbiomes between Individuals with Post Fever Retinitis and Healthy Controls

2020 ◽  
Vol 8 (5) ◽  
pp. 751
Author(s):  
Kotakonda Arunasri ◽  
Malleswarapu Mahesh ◽  
Gumpili Sai Prashanthi ◽  
Rajagopalaboopathi Jayasudha ◽  
Sama Kalyana Chakravarthy ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Inflammatory Diseases ◽  
Control Group ◽  
Metagenomic Sequencing ◽  
Recent Past ◽  
Vitreous Fluid ◽  
Antimicrobial Function ◽  
Microbiome Research ◽  
Bacterial Microbiome ◽  
Health And Disease

Ocular microbiome research has gained momentum in the recent past and has provided new insights into health and disease conditions. However, studies on sight threatening intraocular inflammatory diseases have remained untouched. In the present study, we attempted to identify the bacterial microbiome associated with post fever retinitis using a metagenomic sequencing approach. For this purpose, bacterial ocular microbiomes were generated from vitreous samples collected from control individuals (VC, n = 19) and individuals with post fever retinitis (PFR, n = 9), and analysed. The results revealed 18 discriminative genera in the microbiomes of the two cohorts out of which 16 genera were enriched in VC and the remaining two in PFR group. These discriminative genera were inferred to have antimicrobial, anti-inflammatory, and probiotic function. Only two pathogenic bacteria were differentially abundant in 20% of the PFR samples. PCoA and heatmap analysis showed that the vitreous microbiomes of VC and PFR formed two distinct clusters indicating dysbiosis in the vitreous bacterial microbiomes. Functional assignments and network analysis also revealed that the vitreous bacterial microbiomes in the control group exhibited more evenness in the bacterial diversity and several bacteria had antimicrobial function compared to the PFR group.

scholarly journals Harnessing the strategy of metagenomics for exploring the intestinal microecology of sable (Martes zibellina), the national first-level protected animal

2020 ◽  
Author(s):  
Jiakuo Yan ◽  
Xiaoyang Wu ◽  
Jun Chen ◽  
Yao Chen ◽  
Honghai Zhang
Keyword(s):  
Information Processing ◽  
Intestinal Flora ◽  
Metagenomic Library ◽  
Original Data ◽  
Unique Function ◽  
Metagenomic Data ◽  
Data Mapping ◽  
Metagenomic Sequencing ◽  
Illumina Hiseq ◽  
Martes Zibellina

Abstract Sable (Martes zibellina), belongs to Carnivora, Mustelidae and Maretes, was mainly distributed among the cold northern zone of Eurasia. The purpose of this study is to explore the intestinal flora of the sable by the method of the metagenomic library-based technique, libraries were sequenced on an Illumina HiSeq 4000 instrument. Effective Data volume of each sample is above 6000M, the ratio of the Effective Data (the Clean Data) to original Data (Raw Data) is over 98%. According to the analysis of statistical data, the Total length of ORF is about 603,031, which is 347.36 Mbp. We contrast the unique function of genes with KEGG database, we acquire 7140 genes (KO), a total of all the samples KO is 129788. We selected higher abundance genes to draw cluster heat maps, and according to the results of the KEGG metabolic pathway annotations, we acquire the gene function，including metabolism, environmental information processing, genetic information processing, cellular process and organismal systems. We contrast the unique function of genes with CAZy database, the functional carbohydrate hydrolases have corresponding genes in the intestinal microorganisms of the sable. This is closely related to the fact that the sable is adapted to cold environments and requires a large amount of energy to maintain its metabolic activity. We contrast the unique function of genes with eggNOG database，the main functions of genes included gene duplication, recombination and repair, transport and metabolism of amino acids, transport and metabolism of carbohydrates, etc. In this study, we intended to identify the complex microbial population structure of sables based on metagenomic sequencing method, which uses the whole metagenomic data, mapping the sequences to the known genes or the pathways in the existing databases, such as CAZy, KEGG, or eggNOG, and then exploring the genetic composition and functional diversity of microbial community based on the mapped functional categories.

scholarly journals Harnessing the strategy of metagenomics for exploring the intestinal microecology of sable (Martes zibellina), the national first-level protected animal

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jiakuo Yan ◽  
Xiaoyang Wu ◽  
Jun Chen ◽  
Yao Chen ◽  
Honghai Zhang
Keyword(s):  
Information Processing ◽  
Complex Structure ◽  
Intestinal Flora ◽  
Metagenomic Library ◽  
Metagenomic Data ◽  
Metagenomic Sequencing ◽  
Sequencing Data ◽  
Illumina Hiseq ◽  
Martes Zibellina ◽  
Gene Functions

Abstract Sable (Martes zibellina), a member of family Mustelidae, order Carnivora, is primarily distributed in the cold northern zone of Eurasia. The purpose of this study was to explore the intestinal flora of the sable by metagenomic library-based techniques. Libraries were sequenced on an Illumina HiSeq 4000 instrument. The effective sequencing data of each sample was above 6000 M, and the ratio of clean reads to raw reads was over 98%. The total ORF length was approximately 603,031, equivalent to 347.36 Mbp. We investigated gene functions with the KEGG database and identified 7140 KEGG ortholog (KO) groups comprising 129,788 genes across all of the samples. We selected a subset of genes with the highest abundances to construct cluster heat maps. From the results of the KEGG metabolic pathway annotations, we acquired information on gene functions, as represented by the categories of metabolism, environmental information processing, genetic information processing, cellular processes and organismal systems. We then investigated gene function with the CAZy database and identified functional carbohydrate hydrolases corresponding to genes in the intestinal microorganisms of sable. This finding is consistent with the fact that the sable is adapted to cold environments and requires a large amount of energy to maintain its metabolic activity. We also investigated gene functions with the eggNOG database; the main functions of genes included gene duplication, recombination and repair, transport and metabolism of amino acids, and transport and metabolism of carbohydrates. In this study, we attempted to identify the complex structure of the microbial population of sable based on metagenomic sequencing methods, which use whole metagenomic data, and to map the obtained sequences to known genes or pathways in existing databases, such as CAZy, KEGG, and eggNOG. We then explored the genetic composition and functional diversity of the microbial community based on the mapped functional categories.

scholarly journals Characteristics and variation of fecal bacterial communities and functions in isolated systolic and diastolic hypertensive patients

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Pan Wang ◽  
Ying Dong ◽  
Kun Zuo ◽  
Chunming Han ◽  
Jie Jiao ◽  
...  
Keyword(s):  
Statistical Significance ◽  
Intestinal Flora ◽  
Taxonomic Composition ◽  
Shannon Index ◽  
Control Group ◽  
Fecal Bacteria ◽  
Sequencing Analysis ◽  
Metagenomic Sequencing ◽  
Β Diversity ◽  
Cardiovascular Morbidity And Mortality

Abstract Background Hypertension (HTN) is one of the major cardiovascular risk factors, which contributes to increasing target organ damages and cardiovascular morbidity and mortality worldwide. Isolated systolic HTN (ISH) and isolated diastolic HTN (IDH) are two important subtypes of HTN. Previous researches have demonstrated the alteration of fecal bacteria in HTN, but not down to these two sub-types. In order to identify whether the composition of bacterial taxa and functional modules shift in ISH and IDH, we performed a metagenomic sequencing analysis of fecal samples from 15 controls, 14 ISH, and 11 IDH. Results Compared with control and ISH, IDH patients showed decreased gene number, bacterial richness, and evenness, although the bacterial alterations did not reach statistical significance in the Shannon index. Also, at the genus level, the β-diversity for intestinal flora in IDH was distinguishable from those with ISH. Furthermore, the taxonomic composition of ISH or IDH was different from that of healthy control at genus and species levels. Patients with IDH or ISH were confirmed to be enriched with Rothia mucilaginosa, along with reduced Clostridium sp. ASBs410. Lastly, the altered KEGG modules were significantly decreased in IDH compared with the control group, such as sodium transport system; while for ISH, functions relevant to biotin biosynthesis were decreased. Conclusions Overall, our results showed the disordered fecal bacteria profiles in subjects with ISH and especially IDH, emphasizing the significance of early intervention for IDH.

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