Association of 8p22-23-rs2254546 Genotypes with Coronary Artery Status and Resistance to Intravenous Immunoglobulin in Iranian Children with Kawasaki Disease

Background: Kawasaki disease (KD) is a frequent cause of acquired heart disease in infants and young children, which may be triggered by infectious agents, with the highest incidence in Asian countries. Several genotypes are identified as susceptibility genes for KD, but none is known in the Iranian population. Objectives: Due to the racial differences in the frequency and significance of the identified genes, we aimed to investigate the 8p22-23-rs2254546 genotypes in a sample of Iranian children and their association with aneurysm and resistance to treatment. Methods: In this prospective cross-sectional study, 100 children with KD as the sample group were compared with 100 matched unrelated healthy Iranian children with no history of KD or immune-related disease. The control subjects were ethnically recruited from the same hospital at the time of a routine physical examination. The 8p22-23-rs2254546 genotypes were analyzed using the polymerase chain reaction (PCR) and direct sequencing techniques, and the frequency of the three genotypes (GA, AA, and GG) was reported. Finally, the frequency of aneurysm and IVIG resistance was reported, and their associations with 8p22-23-rs2254546 genotypes were tested by SPSS software. Results: Of 100 patients with a mean age of 1.9 ± 1.7 (0.1 - 10.2) years, 57% were boys and 43% were girls. Of them, 62% had GG, 30% GA, and 8% AA genotypes. Besides, 21% of the studied patients were resistant to IVIG, and 19% had aneurysm, but resistance to IVIG and aneurysm was not associated with GG and GA genotypes of 8p22-23-rs2254546 (P = 0.29 and 0.88, respectively). Conclusions: The majority of the children with KD were shown to have this genetic susceptibility, which shows the importance of the 8p22-23-rs2254546 genotype in Iranian children. However, this genotype was not associated with the risk of resistance to IVIG and aneurysm.

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Comparison of peak expiratory flow measurement by Mini-Wright peak flow meter and electronic spirometer in healthy elementary school children

Paediatrica Indonesiana ◽

10.14238/pi44.6.2004.248-52 ◽

2016 ◽

Vol 44 (6) ◽

pp. 248

Author(s):

Mardjanis Said ◽

Sudigdo Sastroasmoro ◽

Bambang Supriyatno ◽

Yovita Ananta

Keyword(s):

Peak Expiratory Flow ◽

Peak Flow ◽

Method Comparison ◽

Cross Sectional Study ◽

Flow Meter ◽

Cross Sectional ◽

History Of ◽

Routine Physical Examination ◽

The Subject ◽

Expiratory Flow

Objective This study aims to compare peak expiratory flow mea-surement by peak flow meter and electronic spirometer in healthyelementary school children.Methods This was a cross-sectional study performed in an el-ementary school near Cipto Mangunkusumo hospital (SDNPegangsaan 01). The study group consisted of healthy childrenaged 6-12 year old. Data regarding identity and history of illnesswas taken in each subject. Routine physical examination was per-formed and recorded. Each subject performed lung function testsby means of electronic spirometer AS-7 as well as by Mini-Wrightpeak flow meter. The subject should perform a minimum of threemaneuvers for each method where only the best result was re-corded. Method comparison test to assess agreement betweentwo methods was employed in this study.Results There were 10 males and 15 females enrolled in this study.None of the subjects currently have any respiratory symptoms andsigns. This study found that the mean peak expiratory flow (PEF) byspirometer was 226.8±73.13 L/min while by peak flow meter was223.0±45.05. Mean difference between spirometer and peak flowmeter measurements is 6.2 with standard deviation of 60.82. Thesevalues resulted in limits of agreement of -115.44 to +127.84 L/min.Conclusions This study finds disagreement between electronicspirometer and Mini-Wright peak flow meter in measuring peakexpiratory flow, therefore these two devices cannot be usedinterchangeably. Mini-Wright peak flow meter still has a role inhome monitoring, but the physician should interpret the resultscarefully.

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Measles and Rubella Serosusceptibity among Population Vaccinated with Different Schedule: The potential impact on Measles-Rubella elimination in Iran.

10.21203/rs.3.rs-40094/v3 ◽

2021 ◽

Author(s):

Hana Saffar ◽

Maryam Khalifeloo ◽

mohammed-Jafar Saffar ◽

Ali-Reza Abdollahi ◽

Mohammad-Reza Parsaei ◽

...

Keyword(s):

Age Groups ◽

Cross Sectional Study ◽

Relative Distribution ◽

Mmr Vaccine ◽

High Coverage ◽

Cross Sectional ◽

School Entrance ◽

History Of ◽

Measles Vaccination ◽

Iranian Children

Abstract Background: Iranian children have been vaccinated with the scheduled two doses of monovalent measles vaccine (mMV) since 1984. In December 2003, a nationwide campaign of measles-rubella (MR) immunization targeted 5-25 years population was established. In 2004, the mMV was replaced with measles- mumps-rubella (MMR) vaccine. Despite the high vaccination coverage, the outbreaks of measles still occurs in the country. In this Study, the measles-rubella immunity status of various age groups, vaccinated with different schedules was investigated, and the immunologic response of seronegative subjects to revaccination was examined. Methods: This cross-sectional study was conducted among 7- 33-year-old healthy individuals with a documented history of measles vaccination from November 2017-to June 2018. The subjects were categorized as fallow: groupA: including 20-33 years-old; vaccinated with 1-2 dose of mMV, and revaccinated with MR, groupB, including 15-19-year-old individuals, vaccinated with two doses of mMV at nine and 15 months of age, and in addition to one dose MMR upon school entrance, groupC, including 11-14 year-old individuals vaccinated, with two-doses of MMR at the ages of 15 months and six years, and groupD, including 7-10 year individuals vaccinated with two-doses of MMR vaccine at the ages 12 and 18 months, respectively. Nest the consecrations of antimeasles-antirubella IgG antibodies in the collected sera were measured. Among seronegative subjects, the antimeasles-antirubella IgM and IgG were reexamined at 4-6 weeks after MMR revaccination. The collected data were analyzed using descriptive statistical methods.Results: A total of 635 individuals, including 322 females were investigated in this study. The relative distribution of subjects in each group was as falows: groupA, 98; groupB, 295; groupC, 139; and groupD, 103 persons. Overall, 12.3% and 18.4% of the population were seronegative for measles and rubella antibodies. This rate varied greatly between the 4 groups: groupA, 2%-0/0%; groupB,15.2%- 25.0%; groupC,11.5%- 17.2%; and groupD,14.6%- 18.4%. After revaccination, 92% and 94.9% of seronegative individuals only showed IgG response to measles and rubella vaccines, respectively.Conclusion: Despite the high coverage rate of M-R containing vaccines, a significant numbers of vaccinated subjects were seronegative for measles and rubella, possibly because of secondary vaccine failure; this may negatively affect measles-rubella elimination targets in the country. If these findings are confirmed in similar future studies, a more robust regional/national supplementary immunization activity should be considered.

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Measles and Rubella Serosusceptibity Among Population Vaccinated with Different Schedule of Vaccination: The Potential Impact on Measles Elimination in Iran.

10.21203/rs.3.rs-40094/v1 ◽

2020 ◽

Author(s):

Hana Saffar ◽

Maryam Khalifeloo ◽

Mohammed-Jafar Saffar ◽

Ali-Reza Abdollahi ◽

Mohammad-Reza Parsaei ◽

...

Keyword(s):

Age Groups ◽

Cross Sectional Study ◽

Healthy Population ◽

Relative Distribution ◽

Igg Antibodies ◽

Mmr Vaccine ◽

High Coverage ◽

Cross Sectional ◽

History Of ◽

Iranian Children

Abstract Background: In addition to schedule 2-doses monovalent measles vaccine (mMV) immunization of Iranian children since 1984, a nationwide campaign of measles-rubella (MR) immunization among 5- 25 years-old population in December 2003 was conducted. From 2004 mMV was replaced with measles- mumps-rubella (MMR) vaccine. Despite a high vaccination coverage, outbreaks of measles occurred in the country. Study was designed to investigate seroimmunity against measles and rubella among various age groups of population who were vaccinated with different schedule since 1984. Also, immunologic response to revaccination in seronegative subjects was evaluated. Methods: From 1 November 2017 to 30 June 2018 a cross- sectional study among 7- 33 year old healthy population with documented history of measles vaccination was conducted. Based on their age and history of vaccination categorized as GA: 20-23 years old; vaccinated with 1-2 dose of mMV, and also MR revaccinated. GB: 15-19 years, vaccinated only with 2- doses of mMV at the ages of 9 and 15 months. GC: 12-14 years and GD: 7-11 years; vaccinated with 2- dose of MMR vaccine at the ages 15 months - 6 years, and 12-18 month respectively. Collected sera were assessed to measure antimeasles and antirubella IgG antibodies concentration. Four to 6 weeks after revaccination of seronegative subjects, antimeasles-antirubella IgM and IgG antibodies were rechecked. Collected data were analyzed using descriptive statistical methods.Results: Totally 635 individuals, 312 female were included. Relative distribution of subjects in each group was as: GA: 98, GB: 295, GC: 139, and GD: 103 persons. Overall, 12.28% and 18.4% of population were soronegative, and varied greatly between groups: 2%-0/0%, 15.2%- 25.0%, 11.5%- 17.2%; and 14.6%-18.4% to measles and rubella, respectively. After revaccination, 92% and 94.9% showed 1gG response to measles and rubella vaccine respectively.Conclusion: Despite high coverage rate with measles containing vaccine, a significant numbers of vaccinated subjects lost their seroprotection, possibly because of secondary vaccine failure. This may affect measles-rubella elimination goal in the country. If these data were confirmed by further studies, more strengthen regional/ national supplementary immunization activity should be considered.

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Measles and Rubella Serosusceptibity among Population Vaccinated with Different Schedule of Vaccination: the potential impact on measles elimination in Iran.

10.21203/rs.3.rs-40094/v2 ◽

2020 ◽

Author(s):

Hana Saffar ◽

Maryam Khalifeloo ◽

mohammed-Jafar Saffar ◽

Ali-Reza Abdollahi ◽

Mohammad-Reza Parsaei ◽

...

Keyword(s):

Age Groups ◽

Cross Sectional Study ◽

Healthy Population ◽

Relative Distribution ◽

Igg Antibodies ◽

Mmr Vaccine ◽

High Coverage ◽

Cross Sectional ◽

History Of ◽

Iranian Children

Abstract Background: In addition to scheduled 2-doses monovalent measles vaccine (mMV) immunization of Iranian children since 1984, a nationwide campaign of measles-rubella (MR) immunization among 5- 25 years-old population in December 2003 was conducted. From 2004 mMV was replaced with measles- mumps-rubella (MMR) vaccine. Despite a high vaccination coverage, outbreaks of measles occurred in the country. Study was designed to investigate seroimmunity status against measles and rubella among various age groups of population who were vaccinated with different schedule since 1984. Also, immunologic response to revaccination in seronegative subjects was evaluated. Methods: From 1 November 2017 to 30 June 2018 a cross- sectional study among 7- 33 year old (born 1984-2011) healthy population with documented history of measles vaccination was conducted. Based on their age and history of vaccination status categorized as GA: 20-2333 years old; vaccinated with 1-2 dose of mMV, and also MR revaccinated. GB: 15-19 years, vaccinated only with 2- doses of mMV at the ages of 9 and 15 months and MMR 2-5 years later. GC: 12-14 years and GD: 7-11 years; vaccinated with 2- dose of MMR vaccine at the ages 15 months - 6 years, and 12-18 months respectively. Collected sera were assessed to measure antimeasles and antirubella IgG antibodies concentration. Four to 6 weeks after revaccination of seronegative subjects, antimeasles-antirubella IgM and IgG antibodies were rechecked. Collected data were analyzed using descriptive statistical methods.Results: Totally 635 individuals, 312 female were included. Relative distribution of subjects in each group was as: GA: 98, GB: 295, GC: 139, and GD: 103 persons. Overall, 12.28% and 18.4% of population were soronegative, and varied greatly between groups: 2%-0/0%, 15.2%- 25.0%, 11.5%- 17.2%; and 14.6%-18.4%, to measles and rubella, respectively. After revaccination, 92% and 94.9% showed 1gG response to measles and rubella vaccine respectively.Conclusion: Despite high coverage rate with measles and rubella containing vaccine, a significant numbers of vaccinated subjects lost their seroprotection were seronegative, possibly because of secondary vaccine failure. This may affect measles-rubella elimination goal in the country. If these data were confirmed by further studies, more strengthen regional/ national supplementary immunization activity should be considered.

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Minimum acceptable diet and associated factors among infants and young children aged 6–23 months in Amhara region, Central Ethiopia: community-based cross-sectional study

BMJ Open ◽

10.1136/bmjopen-2020-044284 ◽

2021 ◽

Vol 11 (5) ◽

pp. e044284

Author(s):

Abebaw Molla ◽

Gudina Egata ◽

Lemma Getacher ◽

Bezie Kebede ◽

Alemayehu Sayih ◽

...

Keyword(s):

Young Children ◽

Associated Factors ◽

Cross Sectional Study ◽

Home Garden ◽

Growth Monitoring ◽

Sectional Study ◽

Community Based ◽

Cross Sectional ◽

History Of ◽

Infants And Young Children

ObjectiveThe main objective of this study was to assess the prevalence of a minimum acceptable diet (MAD) and associated factors.DesignCommunity-based cross-sectional studySettingDebre Berhan Town, Ethiopia.ParticipantsAn aggregate of 531 infants and young children mother/caregiver pairs participated in this study. A one-stage cluster sampling method was used to select study participants and clusters were selected using a lottery method. Descriptive statistics were calculated for all study variables. Statistical analysis was performed on data to determine which variables are associated with MAD and the results of the adjusted OR with 95% CI. P value of <0.05 considered statistically significant.Primary outcomePrevalence of MAD and associated factorsResultsThe overall prevalence of MAD was 31.6% (95% CI: 27.7 to 35.2). Having mother attending secondary (adjusted OR, AOR=4.9, 95% CI: 1.3 to 18.9) and college education (AOR=6.4, 95% CI: 1.5 to 26.6), paternal primary education (AOR=1.3, 95% CI: 1.5 to 2.4), grouped in the aged group of 12–17 months (AOR=1.8, 95% CI: (1.0 to 3.4) and 18–23 months (AOR=2.2, 95% CI: 1.2 to 3.9), having four antenatal care (ANC) visits (AOR=2.0, 95% CI: 1.0 to 3.9), utilising growth monitoring (AOR=1.8, 95% CI: 1.1 to 2.9), no history of illness 2 weeks before the survey (AOR=2.9, 95% CI: 1.5 to 6.0) and living in the household with home garden (AOR=2.5, 95% CI: 1.5 to 4.3) were positively associated with increase the odds of MAD.ConclusionGenerally, the result of this study showed that the prevalence of minimum acceptable was very low. Parent educational status, ANC visits, infant and young child feeding advice, child growth monitoring practice, age of a child, a child has no history of illness 2 weeks before the survey, and home gardening practice were the predictors of MAD. Therefore, comprehensive intervention strategies suitable to the local context are required to improve the provision of MAD.

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Suicidal Ideation Among Medical Students in Dammam, Saudi Arabia

Crisis ◽

10.1027/0227-5910/a000720 ◽

2020 ◽

pp. 1-6

Author(s):

Mohammed Madadin ◽

Ritesh G. Menezes ◽

Maha A. Alassaf ◽

Abdulaziz M. Almulhim ◽

Mahdi S. Abumadini ◽

...

Keyword(s):

Saudi Arabia ◽

Medical Students ◽

Suicidal Ideation ◽

General Health Questionnaire ◽

Cross Sectional Study ◽

Eastern Province ◽

Cross Sectional ◽

The Past ◽

Depression Subscale ◽

History Of

Abstract. Background: Medical students are at high risk of suicidal ideation. Aim: We aimed to obtain information on suicidal ideation among medical students in Dammam located in the Eastern Province of Saudi Arabia. Method: This cross-sectional study was conducted at the College of Medicine affiliated with Imam Abdulrahman Bin Faisal University in the Eastern Province of Saudi Arabia. Suicidal ideation in the past 12 months was assessed based on responses to four questions in the depression subscale of the General Health Questionnaire 28 (GHQ-28). In addition, data were collected to examine the association of suicidal ideation with various factors. Results: We found that 1 in 3 medical students in the study had suicidal ideation in the past 12 months, while around 40% had lifetime suicidal ideation. Suicidal ideation was associated with feelings of parental neglect, history of physical abuse, and dissatisfaction with academic performance. Limitations: The cross-sectional nature of this study limits its ability to determine causality regarding suicidal ideation. Conclusion: These rates are considerably high when compared with rates from studies in other countries around the world. This study provides a reference in the field of suicidology for this region of Saudi Arabia.

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DYSLIPIDEMIA IN THYROID DISORDERS

International Journal of Medical and Biomedical Studies ◽

10.32553/ijmbs.v4i7.1308 ◽

2020 ◽

Vol 4 (7) ◽

Author(s):

Kashish Narula ◽

Narendra Kumar Dara ◽

Shyam Lal Meena

Keyword(s):

Lipid Metabolism ◽

Thyroid Dysfunction ◽

Cross Sectional Study ◽

Control Group ◽

Thyroid Disorders ◽

Cross Sectional ◽

Carbohydrate And Lipid Metabolism ◽

History Of ◽

Thyroid Profile ◽

Basal Energy Expenditure

Background: Thyroid hormones influence nearly all major metabolic pathways. Their most obvious and well-known action is the increase in basal energy expenditure obtained by acting on protein, carbohydrate and lipid metabolism. The lipid metabolism is more influenced by the thyroid hormone. Methods: A cross-sectional study was conducted on 100 patients with suspicion of thyroid disorders were taken as cases. One hundred patients with normal thyroid profile and no history of other chronic diseases were taken as control group. Results: The serum TC, TG and LDL levels in hypothyroid individuals (both overt and subclinical) were significantly higher than euthyroid subjects but the levels were comparable between hyperthyroid and euthyroid group. Conclusion: Dyslipidemias are associated with thyroid disorders, so biochemical screening for thyroid dysfunction in all dyslipidemic patients. Therefore, patients presenting with dyslipidemia are recommended for investigation to explore thyroid dysfunction. Keywords: Thyroid profile, Total cholesterol, Triglycerides and LDL

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Metabolic Syndrome in HIV-patients in Antiretroviral Therapy

Current HIV Research ◽

10.2174/1570162x18666200609115615 ◽

2020 ◽

Vol 18 (6) ◽

pp. 388-395

Author(s):

Daniel Vargas-Pacherrez ◽

Helma P. Cotrim ◽

Leonardo Pires ◽

Vitor Cunha ◽

Vitor Coelho ◽

...

Keyword(s):

Metabolic Syndrome ◽

Antiretroviral Therapy ◽

International Diabetes Federation ◽

Cross Sectional Study ◽

People Living With Hiv ◽

Anthropometric Data ◽

Cross Sectional ◽

History Of ◽

Family History Of Hypertension ◽

Living With Hiv

Introduction: The global prevalence of metabolic syndrome (MS) among people living with HIV/AIDS varies from 20% to 33%. Objective: to estimate the prevalence of metabolic syndrome and associated factors in a group of HIV-infected patients on antiretroviral therapy. Methods: This is a cross-sectional study with HIV-infected patients from a reference center in Bahia, Brazil. We evaluated clinical, socio-demographic and anthropometric data. MS was defined according to the guidelines of International Diabetes Federation. Results: We evaluated 152 patients with mean age of 47.3±11.6 years, 59.2% male. The main comorbidities detected were diabetes (3.3%) hypertriglyceridemia (9.3%) and metabolic syndrome (MS,38.2%). Patients with MS were predominantly women (55.2% vs 31.9%; p=0.005), older [52.1 (10.4) vs 44.3 (11.3); p<0.001], and had overweight (74.1% vs 23.4%; p<0.001). After multivariate analysis MS remained associated with age (OR = 1.076; 95% CI: 1.030 – 1.125), female sex (OR = 2.452; 95% CI: 1.114 – 5.374) and family history of hypertension (OR = 3.678; 95% CI: 1.431 – 9.395). Conclusion: Almost half of the HIV-infected patients in Bahia presents with MS which seems to be driven by classical risk factors.

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Comparison of Non-radiographic Axial Spondyloarthritis and Ankylosing Spondyltis from a Single Rheumatology Hospital in Morocco

Current Rheumatology Reviews ◽

10.2174/1573397115666190222195923 ◽

2020 ◽

Vol 16 (3) ◽

pp. 240-244 ◽

Cited By ~ 2

Author(s):

Nessrine Akasbi ◽

Siar Nihad ◽

Zoukal Sofia ◽

El Kohen Khadija ◽

Harzy Taoufik

Keyword(s):

Disease Burden ◽

Axial Spondyloarthritis ◽

Univariate Analysis ◽

Cross Sectional Study ◽

Low Back ◽

New Classification ◽

Cross Sectional ◽

Asas Criteria ◽

History Of ◽

High Level

Background: According to the new classification criteria developed by The Assessment of SpondyloArthritis International Society, patients with axial spondyloarthritis (axSpA) can be classified in 2 subgroups: Patients with radiographic axial spondyloarthritis: ankylosing spondylitis patients (AS) and those with non-radiographic axial spondyloarthritis (nr-axSpA). Objective: The aim of the present study is to describe and discuss the differences and similarities between the two subgroups. Patients and Methods: A cross-sectional study was conducted in a single rheumatology hospital in Morocco. These included patients diagnosed as having axial spondyloarthritis according to ASAS criteria 2010, during a period of 6 years. The AS and the nr-axSpA subgroups were compared for the various axSpA-related variables. Results: Of the 277 patients with a diagnosis of axial SpA who were included in this study, 160 had AS and 117 had nr-axSpA. AS and nr-ax-SpA shared a similar age at diagnosis, similar prevalence of low back pain, lumbar stiffness, extra-articular manifestations, BASDAI and BASFI. In the multivariate analysis, AS patients were mainly male with cervical stiffness, enthesitis, coxitis and high level of ESR (erythrocyte sedimentation rate). The females generally had a family history of SpA and arthritis and were associated to the nr-axSpA form in the univariate analysis. Conclusion: This was the first study to characterise patients with AS and nr-axSpA in Morocco. Consistent with other studies published, this study showed that patients with nr-axSpA and patients with AS shared a comparable degree of disease burden.

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Prevalence of MDR organism (MDRO) carriage in children and their household members in Siem Reap Province, Cambodia

JAC-Antimicrobial Resistance ◽

10.1093/jacamr/dlaa097 ◽

2020 ◽

Vol 2 (4) ◽

Author(s):

Shweta R Singh ◽

Bunsoth Mao ◽

Konstantin Evdokimov ◽

Pisey Tan ◽

Phana Leab ◽

...

Keyword(s):

Final Analysis ◽

Cross Sectional Study ◽

Cross Sectional ◽

Middle Income ◽

E Coli ◽

Middle Income Countries ◽

Siem Reap ◽

High Prevalence ◽

Significant Public Health ◽

History Of

Abstract Background The rising incidence of infections caused by MDR organisms (MDROs) poses a significant public health threat. However, little has been reported regarding community MDRO carriage in low- and middle-income countries. Methods We conducted a cross-sectional study in Siem Reap, Cambodia comparing hospital-associated households, in which an index child (age: 2–14 years) had been hospitalized for at least 48 h in the preceding 2–4 weeks, with matched community households on the same street, in which no other child had a recent history of hospitalization. Participants were interviewed using a survey questionnaire and tested for carriage of MRSA, ESBL-producing Enterobacterales (ESBL-E) and carbapenemase-producing Enterobacterales (CPE) by culture followed by antibiotic susceptibility testing. We used logistic regression analysis to analyse associations between collected variables and MDRO carriage. Results Forty-two pairs of households including 376 participants with 376 nasal swabs and 290 stool specimens were included in final analysis. MRSA was isolated from 26 specimens (6.9%). ESBL-producing Escherichia coli was detected in 269 specimens (92.8%) whereas ESBL-producing Klebsiella pneumoniae was isolated from 128 specimens (44.1%), of which 123 (42.4%) were co-colonized with ESBL-producing E. coli. Six (2.1%) specimens tested positive for CPE (4 E. coli and 2 K. pneumoniae). The prevalence ratios for MRSA, ESBL-producing E. coli and ESBL-producing K. pneumoniae carriage did not differ significantly in hospital-associated households and hospitalized children compared with their counterparts. Conclusions The high prevalence of ESBL-E across both household types suggests that MDRO reservoirs are common in the community. Ongoing genomic analyses will help to understand the epidemiology and course of MDRO spread.

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