Screening endophytic actinobacteria with potential antifungal activity against Bipolaris sorokiniana and growth promotion of wheat seedlings

Streptomyces sp. R18(6) and Streptomyces sp. 6(4) strains were evaluated for their ability to control brown spot and common root rot caused by Bipolaris sorokiniana in wheat crops. The antifungal activity of these isolates was tested using a double-layer assay and culture pairing at 28 °C. Physiological and enzymatic activity performed through siderophore, indole-3-acetic acid, nitrogen fixation and phosphate solubilization tests. The biocontrol of the disease and growth-promoting efficiency of wheat seedlings were assessed using in vivo assays in a greenhouse. In the culture pairing assays, both strains inhibited B. sorokiniana mycelial growth, while in the double-layer only Streptomyces sp. R18(6). Streptomyces sp. 6(4) produced auxin, siderophores, fixed nitrogen and solubilized phosphate, whereas R18(6) did not produce siderophores. In the greenhouse assays, strain R18(6) showed statistical differences in shoot dry mass and root dry mass compared with those of strain 6(4) in the presence of the phytopathogen (P ≤ 0.05), and these results were more evident when the environmental temperature was higher. In the absence of the phytopathogen, Streptomyces sp. 6(4) strain increased the root dry mass compared with that of the control during the same period. Therefore, these isolates can potentially control root rot and brown spotting and may promote the growth of wheat plants.

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Antagonistic Strain Bacillus amyloliquefaciens XZ34-1 for Controlling Bipolaris sorokiniana and Promoting Growth in Wheat

Pathogens ◽

10.3390/pathogens10111526 ◽

2021 ◽

Vol 10 (11) ◽

pp. 1526

Author(s):

Yanjie Yi ◽

Youtian Shan ◽

Shifei Liu ◽

Yanhui Yang ◽

Yang Liu ◽

...

Keyword(s):

Biological Control ◽

Root Rot ◽

Bacillus Amyloliquefaciens ◽

Biological Control Agent ◽

Wheat Yield ◽

Bipolaris Sorokiniana ◽

Common Root ◽

Bioactive Substances ◽

Wheat Seedlings ◽

Common Root Rot

Common root rot, caused by Bipolaris sorokiniana, is one of the most prevalent diseases of wheat and has led to major declines in wheat yield and quality worldwide. Here, strain XZ34-1 was isolated from soil and identified as Bacillus amyloliquefaciens based on the morphological, physiological, biochemical characteristics and 16S rDNA sequence. Culture filtrate (CF) of strain XZ34-1 showed a high inhibition rate against B.sorokiniana and had a broad antifungal spectrum. It also remarkably inhibited the mycelial growth and spore germination of B. sorokiniana. In pot control experiments, the incidence and disease index of common root rot in wheat seedlings were decreased after treatment with CF, and the biological control efficacy was significant, up to 78.24%. Further studies showed XZ34-1 could produce antifungal bioactive substances and had the potential of promoting plant growth. Lipopeptide genes detection with PCR indicated that strain XZ34-1 may produce lipopeptides. Furthermore, activities of defense-related enzymes were enhanced in wheat seedlings after inoculation with B.sorokiniana and treatment with CF, which showed induced resistance could be produced in wheat to resist pathogens. These results reveal that strain XZ34-1 is a promising candidate for application as a biological control agent against B.sorokiniana.

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Transcriptome analysis of activated charcoal-induced growth promotion of wheat seedlings in tissue culture

BMC Genetics ◽

10.1186/s12863-020-00877-9 ◽

2020 ◽

Vol 21 (1) ◽

Author(s):

Fu-shuang Dong ◽

Meng-yu lv ◽

Jin-ping Wang ◽

Xue-ping Shi ◽

Xin-xia Liang ◽

...

Keyword(s):

Tissue Culture ◽

Transcriptome Analysis ◽

Activated Charcoal ◽

Growth Promotion ◽

Wheat Seedlings

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Growth promotion of wheat seedlings by Exiguobacterium acetylicum 1P (MTCC 8707) a cold tolerant bacterial strain from the Uttarakhand Himalayas

Indian Journal of Microbiology ◽

10.1007/s12088-009-0024-y ◽

2009 ◽

Vol 50 (1) ◽

pp. 50-56 ◽

Cited By ~ 43

Author(s):

G. Selvakumar ◽

S. Kundu ◽

Piyush Joshi ◽

Sehar Nazim ◽

A. D. Gupta ◽

...

Keyword(s):

Bacterial Strain ◽

Growth Promotion ◽

Wheat Seedlings ◽

Exiguobacterium Acetylicum ◽

Cold Tolerant

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SCREENING OF SALT TOLERANT BACTERIA FOR PLANT GROWTH PROMOTION ACTIVITIES AND BIOLOGICAL CONTROL OF RICE BLAST AND SHEATH BLIGHT DISEASE ON MANGROVE RICE

Vietnam Journal of Science and Technology ◽

10.15625/2525-2518/55/1a/12382 ◽

2018 ◽

Vol 55 (1A) ◽

pp. 54

Author(s):

Nguyen Van Minh

Keyword(s):

Antifungal Activity ◽

Plant Growth ◽

Fungal Pathogen ◽

Rice Blast ◽

Root Zone ◽

Growth Promotion ◽

Sheath Blight ◽

Control Treatment ◽

Rice Soil ◽

Model Combination

From 22 rice, soil and water samples collected in the field of Long An and Tien Giang provinces, we isolated and screened 87 strains of bacteria around the root zone and endophytic bacteria. Through testing the ability of plant growth stimulation, the result showed 16 strains were capable of nitrogen fixation, 13 strains were capable of phosphate solubilization, 27 strains were capable of IAA production and 2 strains had all 3 activities. By the dual testing method and the percentage of inhibition method between bacterial and fungal pathogen, LD5 and LS6 strains had the highest antifungal activity against Rhizotocnia sp. CR1 at 94.02 %. TS3 and TĐ13 strains had the highest antifungal activity against Magnaporthe sp. BP3 at 81.74 ± 0.88 % and 80 ± 0.60 %, respectively. Furthermore, there were 6 strains inhibiting both Rhizotocnia sp. CR1 and Magnaporthe sp. BP3 (LĐ5, LS4, LS6, LN1, LN6, TS3). The strains were identified by biochemical methods. The results showed that LD5, LS6 and TS3 were 70.37 % similar to Bacillus thurigiensis, TD13 strain was 70.37 % similar to Bacillus pantothenticus, TD9 strain was 72.72 % similar to Azotobacter vinelandii and TD6 strain was 70.37 % similar to Bacillus subtilis. Regarding the test of activity to stimulate growth in net house model, combination of 4-strain (TD6, TD9, TD13, TS3) had the effect of increasing the length of roots, trunk and weight of rice compared with control treatment. For evaluation of biocontrol of fungal pathogen in net house model, the abilities to control sheath blight in N-2C1 and N-LĐ5 treatment were the highest (40.59 % and 39.06 %, respectively). The ability to control rice blast in N-2C2 treatment was the highest (41.26 %). The ability to biocontrol both sheath blight and rice blast in N-4C treatment was 37.89 %.

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Draft Genome Sequence of Pseudomonas chlororaphis subsp. aurantiaca ARS-38, a Bacterial Strain with Plant Growth Promotion Potential, Isolated from the Rhizosphere of Cotton in Pakistan

Microbiology Resource Announcements ◽

10.1128/mra.01398-19 ◽

2020 ◽

Vol 9 (3) ◽

Cited By ~ 1

Author(s):

Samina Mehnaz ◽

Andreas Bechthold ◽

Harald Gross

Keyword(s):

Antifungal Activity ◽

Plant Growth ◽

Plant Growth Promotion ◽

Growth Promotion ◽

Draft Genome ◽

Production Capacity ◽

Pseudomonas Chlororaphis ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Insight Into

Strain ARS-38 is a potential plant growth-promoting rhizobacterium that exhibits antifungal properties. Here, we report a 6.6-Mb draft genome, which gives insight into the complete secondary metabolite production capacity and reveals genes putatively responsible for its antifungal activity, as well as genes which contribute to plant growth promotion.

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Community Structures and Antifungal Activity of Root-Associated Endophytic Actinobacteria of Healthy and Diseased Soybean

Microorganisms ◽

10.3390/microorganisms7080243 ◽

2019 ◽

Vol 7 (8) ◽

pp. 243 ◽

Cited By ~ 10

Author(s):

Chongxi Liu ◽

Xiaoxin Zhuang ◽

Zhiyin Yu ◽

Zhiyan Wang ◽

Yongjiang Wang ◽

...

Keyword(s):

Antifungal Activity ◽

High Throughput Sequencing ◽

Antagonistic Activity ◽

Diseased Plant ◽

Natural Defense ◽

Independent Analysis ◽

Culture Independent ◽

Potential Reservoir ◽

Endophytic Actinobacteria

The present study was conducted to examine the influence of a pathogen Sclerotinia sclerotiorum (Lib.) de Bary on the actinobacterial community associated with the soybean roots. A total of 70 endophytic actinobacteria were isolated from the surface-sterilized roots of either healthy or diseased soybeans, and they were distributed under 14 genera. Some rare genera, including Rhodococcus, Kribbella, Glycomyces, Saccharothrix, Streptosporangium and Cellulosimicrobium, were endemic to the diseased samples, and the actinobacterial community was more diverse in the diseased samples compared with that in the heathy samples. Culture-independent analysis of root-associated actinobacterial community using the high-throughput sequencing approach also showed similar results. Four Streptomyces strains that were significantly abundant in the diseased samples exhibited strong antagonistic activity with the inhibition percentage of 54.1–87.6%. A bioactivity-guided approach was then employed to isolate and determine the chemical identity of antifungal constituents derived from the four strains. One new maremycin analogue, together with eight known compounds, were detected. All compounds showed significantly antifungal activity against S. sclerotiorum with the 50% inhibition (EC50) values of 49.14–0.21 mg/L. The higher actinobacterial diversity and more antifungal strains associated with roots of diseased plants indicate a possible role of the root-associated actinobacteria in natural defense against phytopathogens. Furthermore, these results also suggest that the root of diseased plant may be a potential reservoir of actinobacteria producing new agroactive compounds.

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Durum Wheat Stress Tolerance Induced by Endophyte Pantoea agglomerans with Genes Contributing to Plant Functions and Secondary Metabolite Arsenal

International Journal of Molecular Sciences ◽

10.3390/ijms20163989 ◽

2019 ◽

Vol 20 (16) ◽

pp. 3989 ◽

Cited By ~ 4

Author(s):

Hafsa Cherif-Silini ◽

Bathini Thissera ◽

Ali Chenari Bouket ◽

Nora Saadaoui ◽

Allaoua Silini ◽

...

Keyword(s):

Salt Stress ◽

Plant Growth ◽

Durum Wheat ◽

Growth Promotion ◽

Acc Deaminase ◽

Plant Growth Promoting Rhizobacteria ◽

Pantoea Agglomerans ◽

Phylogenomic Analysis ◽

Wheat Roots ◽

Wheat Seedlings

In the arid region Bou-Saâda at the South of Algeria, durum wheat Triticum durum L. cv Waha production is severely threatened by abiotic stresses, mainly drought and salinity. Plant growth-promoting rhizobacteria (PGPR) hold promising prospects towards sustainable and environmentally-friendly agriculture. Using habitat-adapted symbiosis strategy, the PGPR Pantoea agglomerans strain Pa was recovered from wheat roots sampled in Bou-Saâda, conferred alleviation of salt stress in durum wheat plants and allowed considerable growth in this unhostile environment. Strain Pa showed growth up to 35 °C temperature, 5–10 pH range, and up to 30% polyethylene glycol (PEG), as well as 1 M salt concentration tolerance. Pa strain displayed pertinent plant growth promotion (PGP) features (direct and indirect) such as hormone auxin biosynthesis, production of 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia and phosphate solubilization. PGPR features were stable over wide salt concentrations (0–400 mM). Pa strain was also able to survive in seeds, in the non-sterile and sterile wheat rhizosphere, and was shown to have an endophytic life style. Phylogenomic analysis of strain Pa indicated that Pantoea genus suffers taxonomic imprecision which blurs species delimitation and may have impacted their practical use as biofertilizers. When applied to plants, strain Pa promoted considerable growth of wheat seedlings, high chlorophyll content, lower accumulation of proline, and favored K+ accumulation in the inoculated plants when compared to Na+ in control non-inoculated plants. Metabolomic profiling of strain Pa under one strain many compounds (OSMAC) conditions revealed a wide diversity of secondary metabolites (SM) with interesting salt stress alleviation and PGP activities. All these findings strongly promote the implementation of Pantoea agglomerans strain Pa as an efficient biofertilizer in wheat plants culture in arid and salinity-impacted regions.

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Nocardiopsis dassonvillei strain MB22 from the Algerian Sahara promotes wheat seedlings growth and potentially controls the common root rot pathogen Bipolaris sorokiniana

Journal of Plant Pathology ◽

10.1007/s42161-019-00347-x ◽

2019 ◽

Vol 101 (4) ◽

pp. 1115-1125 ◽

Cited By ~ 1

Author(s):

Khadidja Allali ◽

Yacine Goudjal ◽

Miyada Zamoum ◽

Khaoula Bouznada ◽

Nasserdine Sabaou ◽

...

Keyword(s):

Root Rot ◽

Bipolaris Sorokiniana ◽

Common Root ◽

Wheat Seedlings ◽

Common Root Rot ◽

Algerian Sahara ◽

The Common

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Phosphate solubilization and growth promotion by Pseudomonas fragi CS11RH1 (MTCC 8984), a psychrotolerant bacterium isolated from a high altitude Himalayan rhizosphere

Biologia ◽

10.2478/s11756-009-0041-7 ◽

2009 ◽

Vol 64 (2) ◽

Cited By ~ 60

Author(s):

Govindan Selvakumar ◽

Piyush Joshi ◽

Sehar Nazim ◽

Pankaj Mishra ◽

Jaideep Bisht ◽

...

Keyword(s):

High Altitude ◽

Phosphate Solubilization ◽

Plant Biomass ◽

Growth Promotion ◽

Early Report ◽

Rrna Gene ◽

Pseudomonas Fragi ◽

Wheat Seedlings ◽

Cold Temperatures ◽

Psychrotolerant Bacterium

AbstractPhosphate solubilization and growth promotion by Pseudomonas fragi CS11RH1 (MTCC 8984), a psychrotolerant bacterium isolated from a high altitude garlic rhizosphere from the Indian Himalayas, are reported here. The identity of the isolate was arrived on the basis of its biochemical features and sequencing of the 16S rRNA gene. The isolate grew and solubilized phosphate at temperatures ranging from 4 to 30°C. Besides solubilizing P it produced indole acetic acid (IAA) and hydrogen cyanide (HCN). Seed bacterization with the isolate significantly increased the percent germination, rate of germination, plant biomass and nutrient uptake of wheat seedlings. While Pseudomonas fragi is normally associated with the spoilage of dairy products stored at cold temperatures, this is an early report on the plant growth promoting ability of the bacterium.

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