scholarly journals Comparison of bioremediation capabilities of poultry droppings and avocado pear seed cake in petroleum polluted soil

2018 ◽  
Vol 10 (5) ◽  
pp. 38-45
Author(s):  
O. M. Olatunji ◽  
I. T. Horsfall ◽  
T. H. Ekiyor
2021 ◽  
Vol 20 (1) ◽  
pp. 109-124
Author(s):  
V.C Wokem ◽  
E.D. Momoh

The exploration, production and refining of crude oil has led to severe environmental degradation in the oil producing communities of the Niger Delta region of Nigeria. Enhanced bioremediation of tropical rainforest soil artificially polluted with crude oil, bioaugmented with nitrogen fixing bacteria (NFB) and biostimulated with poultry droppings was carried out ex situ. Soil sample was collected at 15cm depth from tropical rainforest soil of the University of Port Harcourt, Nigeria. The NFB was isolated from roots of leguminous plant Arachis hypogea, identified as Nitrobacter species. Bioaugmentation by application of NFB served as option A, option B (biostimulation by application of poultry droppings), option C (No amendment) served as the control. Bioremediation was monitored for 28 days for interval of 14 days, and determined using the percentage ratio of total petroleum hydrocarbon (TPH) losses for each period to TPH at initial day (day zero). Results of total culturable heterotrophic bacterial (TCHB) counts showed that highest range in option B (1.9×104- 2.4×109Cfu/g) than in option A (7.8×106 -2.29×107Cfu/g) and C (6.75×106 -2.6×107Cfu/g) respectively. Similarly, hydrocarbon utilizing bacterial (HUB) counts had higher range in option B (1.20×105 - 1.9×107Cfu/g) than in option A (8.30×104 - 2.30×105Cfu/g) and option C control (4.3×104 −1.69×105 Cfu/g) respectively. Changes in physicochemical parameters during the study showed reductions in nitrate, phosphate and TPH in all the options expect pH which showed slight increase in option C (6.20-6.24). Characterization and identification for bacteria revealed the following HUB genera Pseudomonas, Citrobacter, Bacillus, Corynebacterium, Micrococcus, Klebsiella, Staphylocuccus and Nitrobacter). The percentage losses in TPH from gas chromatography (GC) results showed the following; option A (44.24%) option B (61.08%) and option C - control (27.28%) respectively. The results from this study showed that option B, the application of poultry droppings as biostimulant was more efficient than the application of NFB in enhanced bioremediation of crude oil polluted soil, hence the use of poultry droppings which is available as organic waste, eco-friendly and cost-effective is recommended asbiostimulant for enhanced bioremediation in environmental cleanup of crude oil impacted-sites of the Niger Delta region of Nigeria. Key Words: Bioremediation, Crude oil polluted-soil, Biostimulation, Bioaugumentation, Poultry droppings, Nitrogen fixing bacteria.


2020 ◽  
Vol 3 ◽  
pp. 7
Author(s):  
Joseph E. Agbaji ◽  
Eucharia O. Nwaichi ◽  
Gideon O. Abu

Background: Environmental sustainability is the driver for finding the optimal bioremediation cocktail with the combination of highly potent hydrocarbonoclastic strains and the nutrient additives that significantly enhance mineralization of crude oil in polluted soil in order to mitigate its deleterious effects on the environment. In this study, four hydrocarbon-degrading bacterial strains were pre-selected from mined rhizobacterial isolates in aged crude oil-contaminated soil.  Method: Agrowaste residues of poultry-droppings, corn chaff, and plantain peel were selected among others for their ability to support high biomass of selected bacterial strains. Baseline proximate analysis was performed on the agrowaste residues. Simplified, one variable at a time (OVAT) was employed in the validation of the variables for optimization using the Multivariate analysis tool of Response Surface Methodology (RSM). To test the significant formulation variables, the Box-Behnken approach using 15 runs design was adopted. Results:  The rate of contaminant removal was observed to fit into a quadratic function. For optimal rate or contaminant removal, the fitted model predicted the optimal formulation cocktail condition to be within 0.54 mg/kg (Corn steep liquor), phosphate 137.49 mg/kg (poultry droppings) and 6.4% inocula for initial TPH of 9744 mg kg-1 and THC of 9641 mg kg-1 contaminant level. The model for the application of the bioremediation product and the variables evaluated had a significant p-value < 0.005 for the attainment of 85 to 96 % of TPH and THC removal after 56 days of treatment. Conclusions:  This study has shown the need to harness the abundant agrowaste nutrients in supporting high throughput rhizobacteria in the formulation of a bioremediation agent suitable for use in the reclamation of oil spill sites in the Niger Delta oil-producing region.


2021 ◽  
Author(s):  
Chinedu Christopher Obieze ◽  
Chioma Blaise Chikere ◽  
Rasheed Adeleke ◽  
Ramganesh Selvarajan ◽  
Khayalethu Ntushelo ◽  
...  

Abstract Hydrocarbon pollution amplified by artisanal refining has led to the devastating destruction of farmlands and fishing settlements of subsistence farmers in the Niger Delta. The relatively slow natural attenuation of these polluted sites is the motivation for this study. The natural response and the influence of intermittent tillage and nutrient addition on bacterial community ecological functions were investigated using 16S rRNA metabarcoding. Representative soil samples were drawn from the surface (0 – 15cm) and subsurface (1m and 1.5m depths) of the polluted site pre-remediation and during remediation on Days 0, 14, 49, 70 and 91. Nutrient in the form of poultry droppings was added to the polluted soil while aeration was improved by tillage at 3-weeks interval throughout the duration of remediation. Total petroleum hydrocarbon was reduced from 93,720 mg kg–1 on timepoint zero to 9,029.76 mg kg–1 on day 91. Alpha diversity analysis revealed that the proportionality (evenness) of bacterial species significantly reduced during remediation. The bacterial community structure during remediation was significantly different compared to the structure pre-remediation. Spearman’s rank correlation revealed that soil pH, total phosphorus and total potassium were the chemical factors that influenced diversity. During remediation, the most responsive bacterial phyla were Proteobacteria and Cyanobacteria while Hydrogenedentes and Spirochaetes were among biomarkers pre-remediation. PICRUSt2-based functional prediction revealed that pre-remediation, pathways for methanogenesis and terpenoids biosynthesis were differentially abundant while high energy-yielding TCA cycle and pathways for both fatty acid biosynthesis and the degradation of aromatic hydrocarbons were differentially abundant during remediation. Overall, the addition of poultry droppings and intermittent tillage improved nutrient availability and this subsequently led to a significant change in the bacterial community structure and the rate of hydrocarbon sequestration. The authors concluded that incorporation of poultry droppings by tillage may serve as a suitable remediation method to reduce hydrocarbon in oil-polluted Niger Delta soils.


2019 ◽  
Vol 18 (6) ◽  
pp. 1297-1309
Author(s):  
Antonis A. Zorpas ◽  
Maria K. Doula ◽  
Vassilis J. Inglezakis ◽  
Jose Pedreno Navvaro ◽  
Dimitrios J. Bilalis

2018 ◽  
Vol 69 (7) ◽  
pp. 1695-1698
Author(s):  
Marin Rusanescu ◽  
Carmen Otilia Rusanescu ◽  
Gheorghe Voicu ◽  
Mihaela Begea

A calcium bentonite from Orasu Nou deposit (Satu Mare Romania) was used as raw material. We have conducted laboratory experiments to determine the influence of bentonite on the degree of heavy metal retention. It has been observed that the rate of retention increases as the heavy metal concentration decreases. Experimental studies have been carried out on metal retention ( Zn) in bentonite. In this paper, we realized laboratory experiments for determining the influence of metal (Zn) on the growth and development of two types of plants (Pelargonium domesticum and Kalanchoe) and the effect of bentonite on the absorption of pollutants. These flowers were planted in unpolluted soil, in heavy metal polluted soil and in heavy metal polluted soil to which bentonite was added to observe the positive effect of bentonite. It has been noticed that the flowers planted in unpolluted soil and polluted with heavy metals to which bentonite has been added, the flowers have flourished, the leaves are still green and the plants whose soils have been polluted with heavy metals began to dry after 6 days, three weeks have yellowish leaves and flowers have dried. Experiments have demonstrated the essential role of bentonite for the removal of heavy metals polluted soil.


2020 ◽  
Vol 51 (6) ◽  
pp. 528-540
Author(s):  
I. V. Lyanguzova ◽  
M. S. Bondarenko ◽  
A. I. Belyaeva ◽  
M. N. Kataeva ◽  
V. Sh. Barkan ◽  
...  

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
M. Azizul Moqsud

AbstractIn this research, bioremediation of tsunami-affected polluted soil has been conducted by using collective microorganisms and recycled waste glass. The Tohoku earthquake, which was a mega earthquake in Japan triggered a huge tsunami on March 11th, 2011 that caused immeasurable damage to the geo-environmental conditions by polluting the soil with heavy metals and excessive salt content. Traditional methods to clean this polluted soil was not possible due to the excess cost and efforts. Laboratory experiments were conducted to examine the capability of bioremediation of saline soil by using recycled waste glass. Different collective microorganisms which were incubated inside the laboratory were used. The electrical conductivity (EC) was measured at different specified depths. It was noticed that the electrical conductivity decreased with the assist of the microbial metabolisms significantly. Collective microorganisms (CM2) were the highly capable to reduce salinity (up to 75%) while using recycled waste glass as their habitat.


2021 ◽  
Vol 14 (13) ◽  
Author(s):  
Fatemeh Mohebzadeh ◽  
Babak Motesharezadeh ◽  
Mohammad Jafari ◽  
Salman Zare ◽  
Maryam Saffari Aman

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