scholarly journals Chemical profile, stability and fungicide activity of oil-in-water nanoemulsion (O / A) incorporated with Ba-har essential oil

2021 ◽  
Vol 43 ◽  
pp. e57
Author(s):  
Maria Giullia Alves Carneiro Felizardo ◽  
Ana Patrícia Matos Pereira ◽  
Thaylanna Pinto de Lima ◽  
Thayane Lopes de Sousa ◽  
Cassiano Vasques Frota Guterres ◽  
...  
Keyword(s):  
Essential Oil ◽  
Total Phenolic Content ◽  
Plant Material ◽  
Fungicidal Activity ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Ionic Surfactant ◽  
Chemical Profile ◽  
Fungicidal Action ◽  
Oil In Water

The increase in strains resistant to conventional antifungals means that there is a need for studies related to alternative therapeutic practices, such as medicinal plants. This study aims to evaluate the chemical profile and fungicidal activity of the nanoemulsion of the essential oil obtained from Ba-har (Syrian pepper). The phytochemical profile of the plant material was performed based on the methodology of Matos (2009). For extraction of essential oil, 100g of plant material was used, with the isolation of essential oil by the hydrodistillation technique conducted at 100 °C / 3h. To quantify the total phenolics present in the OE, the Folin-Ciocalteu method was used. For the total flavonoids, the AlCl3 complexation method was used. The oil-in-water nanoemulsion was formulated by the low-energy method of phase inversion using essential oil, non-ionic surfactant and water, and the obtained nanoemulsion was subjected to thermodynamic stability tests. The essential oils and stable nanoemulsions were subjected to evaluation of the fungicidal action against strains of Aspergillus niger (ATCC 6275), Colletotrichum gloeosporioides (ATCC 96723) and Penicilium chrysogenum (ATCC 10106). The fungicidal activity was performed according to CLSI (2020) using the Broth Dilution method to obtain the Minimum Inhibitory Concentration (MIC) and sowing on agar for Minimum Fungicidal Concentration (CFM). The results obtained for the total phenolic content were quantified at 348.3 mg EAT g-1 and 346.21 mg EQ g-1 for flavonoids. Ba-har essential oil was more efficient in inhibiting A. niger, as it presented the lowest MIC (200 µg mL-1), followed by P. chrysogenum (250 µg mL-1) and later by C. gloeosporioides (300 µg mL-1). The identification of the secondary metabolites present in Ba-har was quite considerable, since they are responsible for the biological properties, thus inspiring the continuity of studies related to its biological activities. As for the total phenolic content and flavonoids present in the essential oil, they indicated the important antioxidant potential. The fungicidal potential of Ba-har oil showed strong inhibition and mortality, however the nanoemulsion product with the essential oil incorporated showed a more efficient action against the pathogenic fungi tested,

scholarly journals Determination of total phenolic content, total flavonoid content and FRAP in culinary herbs in relation to harvest time

2012 ◽  
Vol 60 (1) ◽  
pp. 167-172 ◽  
Author(s):  
Jindřiška Vábková ◽  
J. Neugebauerová
Keyword(s):  
Total Phenolic Content ◽  
Plant Material ◽  
Aerial Part ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Antioxidant Power ◽  
Time Of Harvest

In this study there were evaluated total phenolic and flavonoid contents, and ferric reducing antioxidant power (FRAP)of 3 herbs species, that are commonly used in fresh stage (summer savory –Satureja hortensisL., marjoram –Majorana hortensisM. and thyme –Thymus vulgarisL.) in dependence on time of harvest. The total flavonoid content ranged from 2.36 to 4.10 g of catechin equivalents (CE).100g−1of dry weight (dw) of plant material. The highest average total flavonoids content was ascertained in aerial part of summer savory colected in first harvest (4.10 g CE.100g−1dw) and the lowest in aerial part of summer savory collected in third harvest (2.36 g CE.100g−1dw). The highest total flavonoid content was measured by all tested species in plant material harvested in first time of harvest. The highest total phenolic content was estimated in plant material of marjoram harvested in the second time of harvest (6.74 g gallic acid equivalents (GAE).100g−1dw) and the lowest in aerial part of summer savory harvested in the third time of harvest (4.16 g GAE .100g−1dw). Ferric reducing antioxidant power (FRAP) ranged from 1,13 (summer savory, harvest No. 3) to 1.91 g GAE .100g−1dw (thyme, harvest No. 1). The best results of total flavonoid content, total phenolic content and FRAP were obtained by marjoram. Among harvest times there were the highest contents of measured compound mainly by the samples collected in the first time of harvest.

scholarly journals Biometric and phytochemical variability of roseroot (Rhodiola rosea L.) from field cultivation

2014 ◽  
Vol 60 (1) ◽  
pp. 7-17 ◽  
Author(s):  
Artur Adamczak ◽  
Agnieszka Gryszczyńska ◽  
Waldemar Buchwald
Keyword(s):  
Total Phenolic Content ◽  
Plant Material ◽  
Phenolic Content ◽  
Raw Material ◽  
Rhodiola Rosea ◽  
Total Phenolic ◽  
Total Polyphenols ◽  
Biometric Analysis ◽  
Basic Characteristics ◽  
Clump Size

Summary Roseroot (Rhodiola rosea L.) is an adaptogenic plant, widely used in the traditional medicine of Scandinavia, Russia, China and Mongolia. The aim of the study was to determine the biometric and phytochemical co-variability of this species under field cultivation in western Poland (Plewiska near Poznań). The plant material originated from four-year-old cultivation established twice by rhizome division in autumn 2007 and 2008. In the study, 46 individuals (23 plants in 2011 and in 2012) were used. The biometric analysis included measurements of the basic characteristics of plants related to the size (luxuriance) of clumps, shoots, leaves and rhizomes with roots (raw material). The amounts of total polyphenols (expressed as gallic acid), tannins (expressed as pyrogallol) and flavonoids (expressed as quercetin) were determined spectrophotometrically. The obtained results showed high variation of Rh. rosea, especially in the level of flavonoids (0.01-0.20% DM) and in the weight of raw material (113-1156 g FM/plant). There were observed correlations between the phytochemical (flavonoid and total phenolic content) and biometric (water content, leaf number, shoot and clump size) features.

Formulation, Characterization and Antimicrobial efficacy of Aegle marmelos Essential oil nanogel

2021 ◽  
pp. 3662-3668
Author(s):  
Riham Omar Bakr ◽  
Soumaya Saad Zaghloul ◽  
Reham Ibrahim Amer ◽  
Dalia Abd Elaty Mostafa ◽  
Mahitab Helmy El Bishbishy
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Colorimetric Assay ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Aegle Marmelos ◽  
Phytochemical Study ◽  
Esi Ms

Objective: Aegle marmelos (L.) Correa has been widely used in Indian traditional medicine and has many reported pharmacological activities. The aim of this research was to formulate solid lipid nanoparticles (SLNs) of Aegle oil (AO) that enhanced the beneficial antimicrobial activity of the oil. Methods: The chemical composition of Aegle leaf essential oil was analysed by GC-MS. Additionally, a phytochemical study of A. marmelos methanolic leaf extract was conducted using Folin-Ciocalteu colorimetric assay for determination of total phenolic content as well as ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-ESI-MS-MS) analyses for identification of individual components. Six formulations of AO-loaded SLNs (AO-SLNs) were prepared by a double emulsification method. The particle size, zeta potential (ZP), polydispersibility index (PDI) and drug encapsulation efficiency (EE) of the SLNs were determined. The morphology of the SLNs was observed by transmission electron microscopy (TEM). The antimicrobial activity of AO and AO-SLNs was assessed using disc diffusion method. Results: Thirty-two compounds were identified in the Aegle oil, of which Δ-carene and α-phellandrene were the most abundant (48.14% and 34.14%, respectively). The estimated total phenolic content was 968mg gallic acid equivalents (GAE)/g, while UPLC-ESI-MS-MS led to the tentative characterization of thirteen metabolites. The SLNs showed ZP, PDI and EE 125 ± 0.22nm, –37.85, 0.282, and 92%, respectively. AO and AO-SLNs showed significant antimicrobial activity, and the SLNs could sustain the release of AO from their gel vehicles. Conclusion: Our results provide evidence for the application of AO-SLNs in topical and transdermal delivery systems.

scholarly journals Optimization of Polyphenol Extraction from Allium ampeloprasum var. porrum through Response Surface Methodology

Foods ◽  
2018 ◽  
Vol 7 (10) ◽  
pp. 162 ◽  
Author(s):  
Irini Strati ◽  
George Kostomitsopoulos ◽  
Fotios Lytras ◽  
Panagiotis Zoumpoulakis ◽  
Charalampos Proestos ◽  
...  
Keyword(s):  
Total Phenolic Content ◽  
Plant Material ◽  
Phenolic Content ◽  
Solvent Mixture ◽  
Extraction Time ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Factors Affecting ◽  
Allium Ampeloprasum ◽  
Mediterranean Countries

Allium ampeloprasum var. porrum has been recognized as a rich source of secondary metabolites, including phenolic acids, flavonoids and flavonoid polymers (proanthocyanidins or condensed tannins), with related health benefits. Both parts of Allium ampeloprasum var. porrum (white bulb and pseudostem) are traditionally consumed either as a vegetable or as a condiment in many Mediterranean countries. The aim of the present study was to optimize the extraction conditions of polyphenols from white leek stem and green leek leaf by implementing a Box-Behnken design (BBD). The optimization considered basic factors affecting extraction efficiency, including extraction time, solvent to plant material ratio and solvent mixture composition. Maximum polyphenol yield was achieved at an extraction time of 80 and 100 min for white leek stem and green leek leaf extracts respectively, solvent to plant material ratio of 5:1 (v/w) and methanol to water ratio of 40:60 (v/v), for both leek extracts. Interestingly, higher total phenolic content was found in green leek leaf extracts compared to white leek stem extracts, due to a possible relationship between polyphenol production and sunlight radiation. High correlation values were also observed between total phenolic content and antioxidant-antiradical activity of optimized leek extracts.

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