scholarly journals Antibiotic production by Streptomyces hygroscopicus CH-7 in medium containing Schiff base complexes

2019 ◽  
Vol 73 (2) ◽  
pp. 93-101
Author(s):  
Slavica Ilic ◽  
Sandra Konstantinovic ◽  
Gordana Gojgic-Cvijovic ◽  
Vlada Veljkovic
Keyword(s):  
Crude Glycerol ◽  
Antibiotic Production ◽  
Basal Medium ◽  
Divalent Metal ◽  
Biodiesel Production ◽  
Streptomyces Hygroscopicus ◽  
Schiff Base Complexes ◽  
Spectral Studies ◽  
Divalent Metal Ions ◽  
Modified Media

Influence of a modified media on Hexaene H-85 and Azalomycine B production by Streptomyces hygroscopicus CH-7 was investigated. The amino acid L-tryptophan, as a nitrogen source, was replaced with isatin-3-thiosemicarbazone and its complexes with some divalent metal ions. Isatin-3-thiosemicarbazone was synthesized in crude glycerol obtained as a byproduct in biodiesel production from sunflower oil. The complexes were charac-terized by elemental microanalysis and magnetic susceptibility, as well as, by Atomic absorption(AA), Fourier-transform infrared (FTIR) and Ultraviolet?visible (UV/VIS) spectro-scopic methods. The spectral studies indicated an octahedral geometry for the Mn(II), Fe(II) and Ni(II) complexes and a tetrahedral one for the Zn(II) complex. Comparing to the basal medium, isatin-3-thiosemicarbazone (ITC) and its metal complexes in the concentration of 0.5 g dm-3 showed better results in the antibiotics production. Use of medium supple-mented with the Fe(II) complex resulted in the maximum Hexaene H-85 and Azalomycine B concentrations of 306 ?g cm-3 and 127 ?g cm-3, respectively. Addition of ITC and its complexes changed the morphology of S. hygroscopicus CH-7 from filaments to pellets as a dominant shape in media resulting in higher antibiotic production.

scholarly journals The use of salicylaldehyde derivatives as a nitrogen source for antibiotic production by Streptomyces hygroscopicus CH-7

2017 ◽  
Vol 71 (6) ◽  
pp. 487-494
Author(s):  
Sandra Konstantinovic ◽  
Milica Zlatkovic ◽  
Jovan Ciric ◽  
Slavica Ilic ◽  
Gordana Gojgic-Cvijovic ◽  
...  
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Crude Glycerol ◽  
Antibiotic Production ◽  
Biodiesel Production ◽  
Streptomyces Hygroscopicus ◽  
Spectroscopic Methods ◽  
Salicylaldehyde Derivatives ◽  
The Media ◽  
Derivatives Of

In the present work, four derivatives of salicylaldehyde (salicylaldehyde-hydrazone, phenylhydrazone, semicarbazone and thiosemicarbazone) were synthesized using both conventional (95% ethanol) and green (crude glycerol from biodiesel production) solvents. The obtained compounds were identified by elemental microanalysis, as well as FTIR, UV/Vis and 1H-NMR spectroscopic methods. Yields of 93?98% of the compounds in crude glycerol were achieved within 10?25 min. The derivatives of salicylaldehyde and crude glycerol were used as a nitrogen and carbon source, respectively, in the medium for antibiotic (hexaene H-85 and azalomycine B) production by Streptomyces hygroscopicus CH-7. The highest concentrations of hexaene H-85 and azalomycine B were achieved in the medium containing salicylaldehyde-thiosemicarbazone (198 and 69 ?g/cm3, respectively). Derivatives of salicylaldehyde also impacted the strain morphology. In the media with salicylaldehyde- phenylhydrazone and salicylaldehyde-thiosemicarbazone, S. hygroscopicus CH-7 grew like large dispersive pellets with long twisted filaments that produced the highest yield of the antibiotics.

scholarly journals The impact of isatin derivatives on antibiotic production by Streptomyces hygroscopicus CH-7

2016 ◽  
Vol 70 (2) ◽  
pp. 123-128
Author(s):  
Jovan Ciric ◽  
Sandra Konstantinovic ◽  
Slavica Ilic ◽  
Gordana Gojgic-Cvijovic ◽  
Dragisa Savic ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Sunflower Oil ◽  
Crude Glycerol ◽  
Antibiotic Production ◽  
Biodiesel Production ◽  
Streptomyces Hygroscopicus ◽  
The Impact ◽  
Isatin Derivatives

The effect of isatin derivatives as a nitrogen source on antibiotic (Hexaene H-85 and Azalomycine B) production by Streptomyces hygroscopicus CH-7 was studied. Isatin-3-hydrazone, 5-chloroisatin-3-hydrazone, isatin-3-tosylhydrazone, 5-chloroisatin-3-tosylhydrazone, isatin-3-(4`-hidroxy)benzoilhydrazone and 5-chloroisatin-3-(4`-hidroxy)benzoilhydrazone were synthesized in a crude glycerol, obtained during the biodiesel production from edible sunflower oil. The highest concentration of Hexaene H-85 is achieved with 5-chloroisatin-3-hydrazone (197 mg/cm3) in medium, while isatin-3-hydrazone has a greatest impact on Azalomycine B production (72 ?g/cm3).

scholarly journals The effect of the nitrogen source type on the growth and consumption of crude glycerol by Streptomyces hygroscopicus CH-7

2021 ◽  
Vol 10 (1) ◽  
pp. 41-45
Author(s):  
Slavica Ilić ◽  
Jovan Ćirić ◽  
Gordana Gojgić-Cvijović
Keyword(s):  
Amino Acids ◽  
Sunflower Oil ◽  
Crude Glycerol ◽  
Biomass Concentration ◽  
Nitrogen Sources ◽  
Biodiesel Production ◽  
Streptomyces Hygroscopicus ◽  
Basic Medium ◽  
Source Type ◽  
Glycerol Consumption

In this paper we studied the effect of different amino acids (arginine, tryptophan, tyrosine, and phenylalanine) as nitrogen sources on the growth of actinomycete Streptomyces hygroscopicus CH-7 and the consumption of crude glycerol, obtained as a by-product in the biodiesel production from sunflower oil. The highest biomass concentration (9.5 g/L) was achieved using the basic medium and the medium with tryptophan (9.2 g/L), while the crude glycerol consumption was the highest in the basic medium (5.9 mg/mL) and the medium with phenylalanine (3.3 mg/mL).

Synthesis and Chelation Properties of a New Polymeric Ligand Derived from 1-Amino-2-Naphthol-4-Sulfonic Acid

2015 ◽  
Vol 98 (1) ◽  
pp. 188-191
Author(s):  
Dhanasekaran Manivannan ◽  
Valsala Madhavan Nair Biju
Keyword(s):  
Metal Ions ◽  
Sulfonic Acid ◽  
Divalent Metal ◽  
Chelating Resin ◽  
Spectral Studies ◽  
Divalent Metal Ions ◽  
Solid Sorbent ◽  
Lead And Zinc ◽  
Copper Manganese ◽  
Seawater Samples

Abstract A novel chelating resin for preconcentration of heavy metals from various seawater samples has been developed by condensing 1-amino-2-hydroxy-7-[(4-hydroxyphenyl)diazenyl] naphthalene-4-sulfonic acid (AHDNS) with formaldehyde (1:2 mole ratio) in the presence of oxalic acid as the catalyst. The resin thus obtained was used as a solid sorbent for the separationof divalent metal ions present attrace levels in seawater. The functionalized phenol (AHDNS) was characterized by spectral studies. The polymeric resin AHDNS-formaldehyde (AHDNS-F) obtained by condensing thefunctionalized phenol and formaldehyde was characterized by IR and NMR spectral studies. The chelating property of the AHDNS-F resin towards divalent metal ions was studied as a function of pH and in the presence of electrolyte. The metal uptake properties of the resin were determined by using an atomic absorption spectrophotometer. This procedure was validated for recovery of divalent metal ions from seawater samples. The recoveries of cadmium, cobalt, copper, manganese, lead, and zinc were above 92% under the optimum preconcentration conditions. The LOD was <0.73 μg/L and the RSDs were <2%. Thus, the AHDNS-F resin can be widely used as a solid sorbent for the preconcentration of trace metals at ppm levelsin seawater samples.

Influence of fatty acids and detergents on polyether antibiotic production by Streptomyces hygroscopicus NRRL B-1865

1997 ◽  
Vol 43 (9) ◽  
pp. 879-883 ◽  
Author(s):  
Jamal Mouslim ◽  
Nour-Edine El Haloui ◽  
Lucien David
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Methyl Oleate ◽  
Antibiotic Production ◽  
Methyl Esters ◽  
Basal Medium ◽  
Tween 80 ◽  
Streptomyces Hygroscopicus ◽  
Strong Decrease ◽  
Branched Amino Acids

Addition of pure methyl oleate to a Streptomyces hygroscopicus NRRL B-1865 culture improved polyether antibiotic production, particularly of the closely related nigericin and abierixin, more than did technical methyl oleate. Among the fatty acid constituents of technical methyl oleate, the methyl esters of stearate and particularly linoleate were detrimental to growth and antibiotic production, while the methyl esters of palmitate and palmitoleate had the same effect as technical methyl oleate. The presence of Tween 80 or cyclodextrin in S. hygroscopicus cultures supplemented with methyl oleate increased valine uptake and antibiotic production. Antibiotic production was highest with a mixture of 1% methyl oleate, 0.1% Tween 80, and 0.1% cyclodextrin. The results suggested that the stimulatory effect of methyl oleate might be enhanced by a substance increasing the accumulation of branched amino acids known to be direct precursors of polyether antibiotics. The rate of abierixin synthesis was more important than that of nigericin at the end of the culture. The nigericin/abierixin ratio decreased from 6 in basal medium supplemented with methyl oleate to 2 in basal medium supplemented with methyl oleate, Tween 80, and cyclodextrin. A strong decrease in culture pH seen in the presence of Tween 80 and cyclodextrin might lead to a Michael transformation of nigericin to abierixin.Key words: Streptomyces hygroscopicus, nigericin, abierixin, methyl oleate, Tween 80, cyclodextrin.

Stability constants and spectral studies of complexes of 2-hydroxy-5-methylazobenzene-2′-carboxylic acid with some divalent metal ions

1984 ◽  
Vol 62 (11) ◽  
pp. 2299-2301
Author(s):  
C. Mahalingam ◽  
J. K. Sthapak ◽  
D. D. Sharma ◽  
R. L. Tiwari ◽  
Smita Sthapak
Keyword(s):  
Carboxylic Acid ◽  
Metal Ions ◽  
Metal Complexes ◽  
Stability Constants ◽  
Divalent Metal ◽  
Reflectance Spectra ◽  
Spectral Studies ◽  
Divalent Metal Ions ◽  
The Stability

The stability constants of metal complexes of 2-hydroxy-5-methylazobenzene-2′-carboxylic acid have been determined spectrophotometrically. These follow the Irving–Williams sequence: log KOH(H) = 10.67, log K1(Co) = 9.02, log K1(Ni) = 9.68, log K1(Cu) = 13.37, log K1(Zn) = 7.58, and [Formula: see text]The chelates of Mn(II), Fe(II), Co(II), Ni(II), Cu(II), Zn(II), and [Formula: see text] with the dye have been prepared and characterized on the basis of their ir and reflectance spectra.

scholarly journals Complexes of 2,6-dihydroxybenzoic acid with divalent metal ions: Synthesis, crystal structure, spectral studies, and biological activity enhancement

2016 ◽  
Vol 221 ◽  
pp. 617-623 ◽  
Author(s):  
Shella Permatasari Santoso ◽  
Suryadi Ismadji ◽  
Artik Elisa Angkawijaya ◽  
Felycia Edi Soetaredjo ◽  
Alchris Woo Go ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Biological Activity ◽  
Metal Ions ◽  
Divalent Metal ◽  
Spectral Studies ◽  
Divalent Metal Ions ◽  
Dihydroxybenzoic Acid ◽  
Activity Enhancement

scholarly journals The influence of Schiff base inclusion complexes with β-cyclodextrine on antibiotic production by Streptomyces hygroscopicus CH-7

2015 ◽  
Vol 69 (1) ◽  
pp. 9-15
Author(s):  
Slavica Ilic ◽  
Sandra Konstantinovic ◽  
G. Gojgic-Cvijovic ◽  
Dragisa Savic ◽  
Vlada Veljkovic
Keyword(s):  
Schiff Base ◽  
Inclusion Complex ◽  
Schiff Bases ◽  
Inclusion Complexes ◽  
Fermentation Process ◽  
Antibiotic Production ◽  
Basal Medium ◽  
Streptomyces Hygroscopicus ◽  
Nutrient Media ◽  
The Media

A media consisting of isatin-Schiff bases and its inclusion complexes with ?-cyclodextrine was developed to maximize the production of antibiotics Hexaene H-85 and Azalomycine ? by Streptomyces hygroscopicus CH-7. The media with ?-cyclodextrine inclusion complex of isatin-3-thiosemicarbazone resulted in the maximum antibiotics concentration of 493 ?g/cm3 for Hexaene H-85 and 191 ?g/cm3 for Azalomycine B. The production of Hexaene H-85 and Azalomycine is higher when ?-cyclodextrine complex is added as a nitrogen source, comparing to pure isatin-Schiff base. The maximum concentration of hexaene H-85 in medium with inclusion complex of isatin-Schiff base is 1.4-2.3 times higher than the basal medium. The maximum production of Azalomycine is 2.1-3.4 times higher in media with inclusion complex of Schiff base. During the fermentation process, the nutrient media with ?-cyclodextrine inclusion complexes with isatin-Schiff bases affect the strain morphology, since it is in the form of compact pellets, which are formed from short and long, branched filaments

Interaction of divalent metal ions with the NADP+-malic enzyme from maize leaves

1991 ◽  
Vol 81 (4) ◽  
pp. 462-466 ◽  
Author(s):  
Maria Fabiana Drincovich ◽  
Alberto A. Iglesias ◽  
Carlos S. Andreo
Keyword(s):  
Metal Ions ◽  
Malic Enzyme ◽  
Divalent Metal ◽  
Divalent Metal Ions ◽  
Maize Leaves
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