Exploring the protective effect of Ascorbic acid on Amoxicillin and Clavulanic acid-induced lipid peroxidation in goat liver homogenate

AbstractIn the present study, the induction of oxidative stress was examined in the testis of alloxan-induced diabetic rabbits. In addition, the protective effect of repaglinide, an oral anti-diabetic, at a dose of 1 mg daily was studied after four and eight weeks of the treatment. For these purposes, the levels of superoxide dismutase (Cu,Zn-SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GSSG-R), glutathione (GSH), ascorbic acid (AA), lipid peroxidation products (LPO) and protein carbonyl groups (PCG) were quantified. Hyperglycemia resulted in significant increases in the antioxidative enzymes, Cu, Zn-SOD, CAT, GSH-Px, and GSSG-R after four and eight weeks, respectively. There was also an increase in GSH level, and a decrease in the level of AA. These effects were accompanied by an elevation in testicular LPO levels and PCG levels. Repaglinide was found to normalize the activity of GSSG-R and levels of GSH and AA, and blunted the increased lipid peroxidation, however no decrease in PCG levels were observed. In conclusion, some oxidative changes provoked in the testis of rabbits by hyperglycemia, were found to be reduced with repaglinide treatment at therapeutic dose.

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In Vitro Protective Effect of Ascorbic Acid Against Antibiotic-Induced Hepatotoxicity

Current Drug Discovery Technologies ◽

10.2174/1570163816666190204122007 ◽

2020 ◽

Vol 17 (3) ◽

pp. 357-364 ◽

Cited By ~ 1

Author(s):

Veneta Simon ◽

Sreerag K.V. ◽

R. Sasikumar ◽

S.K. Kanthlal

Keyword(s):

Ascorbic Acid ◽

Vitamin C ◽

Protective Effect ◽

Liver Toxicity ◽

Liver Homogenate ◽

Leafy Vegetables ◽

Cell Viability Assay ◽

Liver Slices ◽

Percentage Survival

Background: Although antibiotic-induced hepatotoxicity is recoverable with mild impairment, and some cases were reported to cause morbidity. However, an adjuvant is essential in reducing such incidences. Objective: The aim of this study is to evaluate the protective effect of ascorbic acid on antibiotic induced liver toxicity using liver slices. Methods: Fresh liver slices were incubated with different concentrations of sulfamethoxazole tetracycline and clavulanic acid along with ascorbic acid (200μg/ml) for 2 hours. The liver homogenate was assessed for markers like ALT, AST, MDA and CAT levels. Cytotoxicity assessment was performed using MTT assay. Results: Incubating liver slices with all three antibiotics shows elevated levels of aminotransferases, MDA and CAT enzyme when compared to the control groups which indicates the level of hepatotoxicity. In the presence of ascorbic acid, the elevated levels of TBARS, ALT and AST were significantly reduced which showcases the protective effect of ascorbic acid. The percentage survival of cell was also shown to have improved while accessed using cell viability assay. Conclusion: Obtained data suggests that consuming vitamin C or vitamin C containing food like citrus fruits or green leafy vegetables equivalent to 3g/day during antibiotic treatment, perhaps put down the risk of liver toxicity to a greater extent.

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Protective effect of ascorbic acid against lipid peroxidation and oxidative damage in cardiac microsomes

Molecular and Cellular Biochemistry ◽

10.1007/bf01772209 ◽

1993 ◽

Vol 126 (1) ◽

pp. 69-75 ◽

Cited By ~ 10

Author(s):

Mau Mukhopadhyay ◽

Chinmay K. Mukhopadhyay ◽

Indu B. Chatterjee

Keyword(s):

Lipid Peroxidation ◽

Ascorbic Acid ◽

Oxidative Damage ◽

Protective Effect

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Effect of selenium on vanadium toxicity in different regions of rat brain

Human & Experimental Toxicology ◽

10.1177/096032719801700104 ◽

1998 ◽

Vol 17 (1) ◽

pp. 23-28 ◽

Cited By ~ 8

Author(s):

Syed Saleem Haider ◽

A A Abdel-Gayoum ◽

Mustafa El-Fakhri ◽

Kilani M Ghwarsha

Keyword(s):

Lipid Peroxidation ◽

Ascorbic Acid ◽

Protective Effect ◽

Rat Brain ◽

Sulfhydryl Group ◽

Brain Regions ◽

Neurotoxic Effects ◽

Selenium Treatment ◽

Vanadium Toxicity ◽

The Brain

The protective effect of selenium on the neurotoxicity of vanadium in different brain regions of rats was investigated. The lipid peroxidation was significantly accentuated after intraperitoneal (i.p.) administration of vanadium (1.5 mg kg71 b.wt) for a period of 12 consecutive days to rats. The increase in lipid peroxidation was inhibited by selenium treatment (0.02 mg kg71 b.wt., i.p.) for 12 consecutive days. Vanadium exposure produced a decrease in nonprotein sulfhydryl group. Selenium treatment prevented the depression in nonprotein sulfhydryl group in all the brain regions of the vanadium exposed rats. The concentration of ascorbic acid was decreased after co-administration of selenium and vanadium. These results suggest that selenium protects neuronal cells against neurotoxic effects of vanadium by maintaining the availability of antioxidant nonprotein sulfhydryl groups. The decrease in ascorbic acid levels may have been due to its consumption in forming complexes with vanadium.

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Total Antioxidant Capacity and Lipid Peroxidation Status in Cervical Cancer Patients Compared with Women Without Cervical Cancer in Bangladesh

Indian Journal of Gynecologic Oncology ◽

10.1007/s40944-021-00560-6 ◽

2021 ◽

Vol 19 (4) ◽

Author(s):

Taslima Nigar ◽

Annekathryn Goodman ◽

Shahana Pervin

Keyword(s):

Oxidative Stress ◽

Cervical Cancer ◽

Lipid Peroxidation ◽

Ascorbic Acid ◽

Antioxidant Capacity ◽

Cancer Patients ◽

Total Antioxidant Capacity ◽

Stress Index ◽

Oxidative Stress Index ◽

Total Antioxidant

Abstract Purpose Over the past several decades, research has suggested reactive oxygen species act as cofactors for cervical cancer development. The aim of this study is to evaluate the antioxidant and lipid peroxidation status in cervical cancer patients in Bangladesh. Methods From December 2017 to 2018, a cross-sectional observational study was conducted on 50 cervical cancer patients and 50 controls. Plasma levels of lipid peroxidation and total antioxidant capacity were measured. The Student’s t test was used for statistical analysis. P values less than 0.05 were taken as a level of significance. Results There was a significant reduction in total antioxidant levels in patients with cervical cancer, 972.77 ± 244.22 SD µmol equivalent to ascorbic acid/L, compared to normal controls, 1720.13 ± 150.81 SD µmol equivalent to ascorbic acid/L (P < 0.001). Levels of lipid peroxidation were found to be significantly higher in cervical cancer, 7.49 ± 2.13 SD µmol/L, than in women without cervical cancer, 3.28 ± 0.58 SD µmol/L (P < 0.001). The cervical cancer patients had significantly higher levels of oxidative stress index (0.83 ± 0.31) in comparison to controls (0.19 ± 0.04) (P < 0.001). Conclusion There was an increased oxidative stress index due to imbalance between lipid peroxidation generation and total antioxidant capacity in cervical cancer patients. Further studies are needed to explore the role of oxidative stress as a cofactor for cervical carcinogenesis.

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