scholarly journals Genetic diversity for kernel yield and qualitative traits in peanut stem necrosis tolerant groundnut genotypes of Andhra Pradesh

2017 ◽  
Vol 8 (1) ◽  
pp. 390
Author(s):  
P. Dharani Niveditha ◽  
M. Sudharani ◽  
A. Prasanna Rajesh
Author(s):  
K. Saratbabu ◽  
K. Vemana ◽  
A.K. Patibanda ◽  
B. Sreekanth ◽  
V. Srinivasa Rao

Background: Peanut stem necrosis disease (PSND) caused by Tobacco streak virus (TSV) is a major constraint for groundnut production in Andhra Pradesh (A.P.). However, studies on prevalence and spread of the disease confined to only few districts of A.P. with this background current study focused on incidence and spread of the disease in entire state of A.P. Further an isolate of TSV occurring in A.P. characterized on the basis of genetic features by comparing with other TSV isolates originated from different hosts and locations from world.Methods: Roving survey was conducted during kharif 2017-18 in groundnut growing districts of Andhra Pradesh (A.P.) for peanut stem necrosis disease incidence. Groundnut plants showing PSND symptoms were collected and tested with direct antigen coating enzyme linked immunosorbent assay (DAC-ELISA). Groundnut samples found positive by ELISA once again tested by reverse transcription polymerase chain reaction (RT-PCR). The representative TSV-GN-INDVP groundnut isolate from Prakasham district was maintained on cowpea seedlings by standard sap inoculation method in glasshouse for further molecular characterization. The Phylogenetic tree for coat protein (CP) gene was constructed using aligned sequences with 1000 bootstrap replicates following neighbor-joining phylogeny.Result: Thirty-eight (52.7%) of seventy-two groundnut samples collected from different locations in A.P were given positive reaction to TSV by DAC-ELISA. For the first time, PSND incidence observed in coastal districts (Krishna, Guntur, Sri Pottisriramulu Nellore, Prakasham) of A.P. Maximum PSND incidence recorded from Bathalapalli (22.2%) and the minimum incidence in Mulakalacheruvu (4.1%). The coat protein (CP) gene of TSV-GN-INDVP groundnut isolate was amplified by RT-PCR and it shared maximum per cent nucleotide identity (97.51-98.62%) with TSV isolates from groundnut and other different crops reported in India. All Indian isolates cluster together irrespective of crop and location based on the phylogenetic analysis.


2017 ◽  
Vol 29 (5) ◽  
pp. 919-943 ◽  
Author(s):  
Rafael A. Cañas ◽  
Zhazira Yesbergenova-Cuny ◽  
Margaret Simons ◽  
Fabien Chardon ◽  
Patrick Armengaud ◽  
...  

2021 ◽  
Vol 17 (40) ◽  
pp. 20
Author(s):  
Wend-Pagnagde Félicien Marie Serge Zida ◽  
Teyoure Benoit Joseph Batieno ◽  
Tinga Jeremy Ouedraogo ◽  
Mahamadou Sawadogo

Mung bean (Vigna radiata L. Wilczek) is an important annual legume which is well suited to dry areas, like Burkina Faso where it is still an orphan legume. The present study was conducted to evaluate 44 mung bean genotypes introduced in Burkina Faso and to determine genetic diversity in this collection using both quantitative and qualitative traits. A field experiment was conducted in an augmented bloc design with three blocks and an extraearly maturing cowpea variety as check. Data were recorded on six (06) quantitative traits and seven (07) qualitative traits. For qualitative traits, diversity was revealed in four parameters including hypocotyl’s color, seed coat color, pods and stem pubescence. Significant genetic variability was revealed among genotypes for all the quantitative characters. Phenotypic coefficient of variation (PCV) was higher than Genotypic coefficient of variation (GCV) for all characters studied indicating the influence of environmental effect on the characters. The GCV and PCV estimates were high for number of pods per plant followed by plant height and hundred seeds weigh. The high heritability coupled with high genetic advanced as percent of mean observed for these traits suggest an important genetic gain in genetic improvement for these characters. The genetic diversity observed was grouped into five clusters. Mungbean lines were grouped into four clusters and the cowpea variety used as check crop in this trial was clustered apart. This genetic diversity, both qualitatively and quantitatively, will help enrich local biodiversity in terms of legumes in general and mung bean in particular and constitutes an important basis for a future mungbean breeding program.


2019 ◽  
Vol 79 (01) ◽  
Author(s):  
Anushree Pramanik ◽  
Sushma Tiwari ◽  
R. S. Tomar ◽  
M. K. Tomar ◽  
A. K. Singh

The genetic assessment of 90 germplasm lines and six varieties of groundnut (Arachis hypogaea L.) were done with 13 morphological traits and 125 Simple Sequence Repeats markers. Out of 125 molecular markers, 26 were polymorphic and produced 105 alleles. The genetic diversity was found to be 52-83 per cent and Polymorphic Information Content (PIC) was 0.46-0.81 with a mean of 0.42 indicating higher magnitude of genetic diversity in the test genotypes. Analysis of molecular variance showed variation among and within individuals based on allelic variation. Principal Co-ordinate Analysis based on origin of the genotypes formed three major population groups and the genetic analysis determined by population structure divided all the germplasm lines in to 10 populations. Significant and positive correlation was observed between hundred kernel weight and hundred pod weight (r=0.769) and kernel yield (r=0.899); sound mature kernel and pod weight with kernel yield, weight of kernels and harvest index. Genotypes from distinct clusters may be selected in hybridization programme for groundnut improvement. The information on clustering of genotypes will be helpful in identification of novel and superior germplasm for hybridization and development of improved varieties.


2017 ◽  
Vol 9 (4) ◽  
pp. 508-514 ◽  
Author(s):  
Toscani NGOMPE-DEFFO ◽  
Eric Bertrand KOUAM ◽  
Honoré BEYEGUE-DJONKO ◽  
Mariette ANOUMAA

Characterization of the genetic diversity and analysis of the genetic relationship between accessions of a crop species is a key step in breeding superior cultivars. The main objective of the hereby study was to determine the genetic variation between 30 cowpea accessions collected throughout the eight divisions of the Western Region of Cameroon using qualitative traits. Phenotypic variation of these accessions was evaluated using diversity indices and cluster analyses. A total of twenty qualitative traits were used for the study. Fifteen of them (75%) were polymorphic, displaying each at least two phenotypic classes. The monomorphic characters were growth pattern, leaf color, leaf hairiness, plant hairiness and pod hairiness, each with only one phenotypic class. Results showed a relatively significant level of genetic diversity among the studied cowpea accessions. Overall, the average of the observed and effective number of phenotypic classes per qualitative trait were Na = 2.350 and Ne = 1.828 respectively. The Nei’s genetic diversity and the Shannon weaver diversity index were He = 0.369, ranging from zero (monomorphic trait) to 0.655 (growth habit) and H’ = 0.609, ranging from zero (monomorphic trait) to 0.996 (seed crowding), respectively. The dendrogram constructed from the twenty qualitative traits revealed 05 accessions clusters with the number of accessions in each cluster varying from one to eleven. Information obtained from this study is likely be useful for future cowpea breeding program.


2019 ◽  
Vol 9 (1) ◽  
pp. 23-34
Author(s):  
Satish Kachare ◽  
Sharad Tiwari ◽  
Niraj Tripathi ◽  
Vishwa Vijay Thakur

2016 ◽  
Vol 7 (4) ◽  
pp. 1089
Author(s):  
CH.V. Durga Rani ◽  
S Vanisri ◽  
Jamaloddin Mohammed ◽  
Swathi Gattu ◽  
Ramprasad Eruvuri ◽  
...  

2020 ◽  
Vol 2 (1) ◽  
pp. 34-50
Author(s):  
Ebrahim Seid Hussen ◽  
Wassu Mohammed Ali ◽  
Tessfaye Abebe Desta

Potato (Solanum tuberosum L.) is a versatile crop and a source of inexpensive energy in the human diet in many countries. It can be used as fresh products and commercially processed foods such as French fries and chips. Potato varieties development research previously conducted in Ethiopia related to processing quality were limited in their scope of quality parameters. This experiment was conducted at Holetta Agricultural Research Centre, Ethiopia during the main crop season of 2017. Twenty-four potato genotypes were evaluated for 23 quantitative and six qualitative traits in randomized complete block design with three replications to determining the nature and magnitude of common genetic diversity and to screen out genetically diverse parents by using cluster and principal component analysis. The first eight principal components accounted for 90.26% of the observed variations among 24 potato genotypes. The first three PC accounted for 60.43% of the variation. The genetic distances among the 24 potato genotypes ranged from 3.40 to 11.80 and the genotypes were grouped into eight clusters based on quantitative and qualitative traits. Cluster II consisted of 25%, Cluster IV, I, III contained 20.83%, 16.67% and 12.5% of genotypes, respectively, while Cluster VI, VII and VIII each consisted of one genotype. In conclusion, genotypes grouped under Cluster II and VIII worth further evaluation to obtain genotypes with highest total tuber yield, the specific gravity of tuber, dry matter content, total starch content, acceptable tuber physical and frying quality with other desirable traits.


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