A Study on Burkholderia Cepacia Isolates from Various Clinical Samples and their Antibiotic Sensitivity Pattern

2017 ◽  
Vol 3 (1) ◽  
pp. 20
Author(s):  
J Tabindah ◽  
N Girish ◽  
R Rajendran
Keyword(s):  
Burkholderia Cepacia ◽  
Antibiotic Sensitivity ◽  
Clinical Samples ◽  
Sensitivity Pattern

scholarly journals Non fermentative gram negative bacilli as nosocomial pathogens: Identification and antibiotic sensitivity in clinical samples of indoor patients

2015 ◽  
Vol 3 (2) ◽  
pp. 101-105 ◽  
Author(s):  
D Sharma ◽  
N Vyas ◽  
P Sinha ◽  
A Mathur
Keyword(s):  
Burkholderia Cepacia ◽  
Treatment Options ◽  
Antibiotic Sensitivity ◽  
Burkholderia Cepacia Complex ◽  
Clinical Samples ◽  
Sensitivity Testing ◽  
Gram Negative ◽  
Nosocomial Pathogens ◽  
Sensitivity Pattern ◽  
Medical Icus

Background: Non fermenting gram negative bacilli (NFGNB) are usually considered to be non pathogenic commensals of little significance, however, data suggest that they are remarkable because of antimicrobial resistance; propensity to cause outbreaks and complex epidemiology. This study was conducted to find out the prevalence and sensitivity pattern of NFGNB from various clinical samples.Methods: A total of 519 samples were received from patients suspected of having hospital acquired infections (HAI), admitted in Sawai Man Singh Hospital, Jaipur, India. Organisms grown on culture were subjected to phenotypic identification along with antimicrobial sensitivity testing by Kirby Bauer’s disc diffusion method.Results: Among 366 culture positive samples, 94 (25.6%) NFGNB were isolated. Maximum pevalence prevalence was found in burn ward i.e. 76.7 % followed by surgical wards 71.0%, surgical ICUs 69.6% and medical ICUs 68.0 %. Highest yield was found in pus/wound samples (95.5%) while it was only 23.7% in blood samples. Pseudomonas aeruginosa was the most common isolate (48.9%), followed by Acinetobacter baumannii (33%), Stenotrophomonas maltophilia (13.8%) and Burkholderia cepacia complex (4.3%). P.aeruginosa and A. baumanii strains were most sensitive to Meropenem. S.maltophilia showed maximum sensitivity with ticarcillin/ clavulanic acid and B. cepacia complex with Piperacillin/ Tazobactam.Conclusion: Isolation of NFGNB and their antibiotic susceptibility pattern should be regarded with all seriousness in clinical practice and epidemiology because they are emerging nosocomial pathogens and by being resistant to multiple antibiotics, their prevalence not only limits the treatment options but also act as a reservoir of drug resistance genes.Nepal Journal of Medical Sciences Vol.3(2) 2014: 101-105

scholarly journals TO STUDY ANTIBIOTIC SENSITIVITY PATTERN OF NONFERMENTATIVE GRAM NEGATIVE BACILLI FROM VARIOUS CLINICAL SAMPLES IN A TERTIARY CARE HOSPITAL, JAIPUR.

2020 ◽  
Vol 4 (5) ◽  
Author(s):  
Kirti Hemwani ◽  
P. S. Nirwan ◽  
Preeti Shrivastava ◽  
Abhiraj Ramchandani
Keyword(s):  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Polymyxin B ◽  
Hospital Environment ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Care Hospital ◽  
Pathogenic Potential ◽  
Gram Negative ◽  
Sensitivity Pattern

Background: Nonfermentative gram negative bacilli (NFGNB) frequently considered as commensals or contaminants but the pathogenic potential of nonfermenters has been proved beyond doubt. They are resistant to commonly used antimicrobials. Aim: This study was undertaken to identify the nonfermenters isolated from various clinical samples and to know their Antibiotic sensitivity pattern. Materials and Methods: The present study was carried out on 150 strains of Nonfermenters isolated from 1200 various non repetitive clinical samples received in Department of Microbiology, NIMS Jaipur. Nonfermenters were identified using a standard protocol and their antibiotic susceptibility testing was performed with the help of the modified Bauer disc diffusion method. Results: Out of 150 nonfermenters isolated, Pseudomonas aeruginosa was the most common isolate 134 (89.33%) followed by Acinetobacter baumannii 16 (10.67%). Among all clinical samples Pus and Wound Discharge yield maximum isolates of NFGNB i.e. 54 (36%) % followed by sputum (39.0%). Most sensitive drug against NFGNB was Polymyxin-B (100%) followed by Imipenem (86 %) and Amikacin (71.33 %). Conclusion: Nonfermenters have a great potential to survive in a hospital environment so implementation of antibiotic stewardship programs and strict infection control practices will be required to prevent or slow down their emergence and spread. Keywords:  Nonfermenters,  Polymyxin-B, Pseudomonas, Acinetobacter.

scholarly journals Prevalence and antibiotic susceptibility pattern of Klebsiella species isolated from various clinical samples in a tertiary care hospital Coimbatore

2021 ◽  
Vol 8 (10) ◽  
pp. 4886
Author(s):  
Vijayashree V. ◽  
Saikeerthana D. ◽  
Prabha P.
Keyword(s):  
Antibiotic Susceptibility ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Clinical Samples ◽  
Care Hospital ◽  
Susceptibility Pattern ◽  
Klebsiella Species ◽  
Antibiotic Susceptibility Pattern ◽  
Sensitivity Pattern

Background: The genus Klebsiella of Enterobacteriaceae family is ubiquitous in nature. They cause many nosocomial infections like pneumonia, urinary tract infections, wound infections, bacteremia and septicemia. Multidrug resistance is seen in Klebsiella which serves as the most common cause of increased morbidity and mortality. This study reveals the prevalence and antibiotic sensitivity pattern of Klebsiella species from various clinical samples. The primary objectives are as follows: To isolate and characterize Klebsiella species from various clinical samples. To study the antibiotic susceptibility pattern of Klebsiella isolates.Methods: This prospective study was conducted in our tertiary care hospital during the period from August 2019 to October 2019. A total of 3521 samples were tested during this period. The samples include blood, sputum, urine, and pus.Results: Out of the total samples tested,1106 samples were showing the growth of the organisms. Among this,351 were identified as Klebsiella species and the highest rate of isolation of Klebsiella species is from the sputum sample and also the same was reported high in medical wards. The study also shows that the isolation of Klebsiella species shows male preponderance when compared to females. The antibiotic sensitivity pattern was done by Kirby-Bauer's disc diffusion method and the sensitivity was noted to be higher to amikacin and ciprofloxacin.Conclusions: Thus, this study shows the prevalence rate of Klebsiella species and sensitivity pattern of Klebsiella, which may help select appropriate antibiotics and prevent overuse and misuse of antibiotics.

scholarly journals Evaluation of Antibiotic Sensitivity Pattern and Plasmid Isolation of Klebsiella Pneumoniae Isolates from Clinical Specimens in Sylhet City, Bangladesh

2017 ◽  
Vol 9 (1) ◽  
pp. 79-86
Author(s):  
P. Bhattacharjee ◽  
M. Z. Alam ◽  
S. M. A. Sayem
Keyword(s):  
Klebsiella Pneumoniae ◽  
Antimicrobial Agents ◽  
Bacterial Pathogen ◽  
Antibiotic Sensitivity ◽  
High Rate ◽  
Multi Drug Resistance ◽  
Clinical Samples ◽  
Antimicrobial Sensitivity ◽  
Microbiological Methods ◽  
Sensitivity Pattern

Klebsiella pneumoniae is recognized as an emerging opportunistic bacterial pathogen of clinical relevance for its association with both community acquired and nosocomial infections. Moreover, increased resistance to common antimicrobial agents complicates and limits available therapeutic options. The aim of this study was to investigate an antibiotic sensitivity pattern of K. pneumoniae isolated from clinical samples against commonly used antibiotics and to focus on the associated therapeutic challenges. Presumptive K. pneumoniae isolates were collected from a variety of sources like urine sample, pus, wound swab etc. and further analyzed through standard microbiological methods. Following the identification of 23 bacterium isolates from the collected specimens, an antimicrobial sensitivity test was carried out. The results of this test indicated that the organisms possess diversity of susceptibility profiles towards the drugs tested. A high rate of resistance to the first line antibiotics like Amoxicillin (100%), Vancomycin (90.90%), Erythromycin (83.33%), and Cotrimoxazole (83.33%), designates a critical therapeutic challenge. However, inspite of a moderate sensitivity to the Gentamicin (43.5%), increased number of intermediate sensitivity to the drugs tested clearly point towards the multi-drug resistance of the K. pneumoniae isolates. Plasmid profiling experiment revealed that the size of the isolated plasmids varied in length, ranging from 800-900bp.

scholarly journals Identification, Speciation and Antibiogram along with Detection of Metallo Beta-lactamase Production in Acinetobacter Isolated from Clinical Samples in a Tertiary Care Hospital

2021 ◽  
Author(s):  
N Ashwin Chitrabanu ◽  
Shrikara Mallya
Keyword(s):  
Acinetobacter Baumannii ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Clinical Samples ◽  
Care Hospital ◽  
Acinetobacter Species ◽  
Carbapenem Resistant ◽  
Appropriate Treatment ◽  
Sensitivity Pattern ◽  
Synergy Test

Acinetobacter species are gram negative non fermenters, which are important nosocomial pathogens involved in various outbreaks in hospitals due to widespread resistance to majority antibiotics. The aim of this study is to speciate Acinetobacter isolated from clinical samples, to assess the antibiotic sensitivity pattern and to detect the production of metallo-β-lactamase by double disc synergy test. The study was conducted in the department of microbiology, A. J Institute of Medical Sciences. All clinical samples were subjected to gram stain & cultured; the Acinetobacter isolates obtained were subjected to antibiogram. Those isolates that showed Imipenem resistant were further tested for production of metallo-β-lactamase by double disc synergy test. Out of 6625 culture positive isolates, 414 (36.1%) were identified biochemically to belong to Acinetobacter species. Of the 414 cases, 393 (94.9%) were further identified to be Acinetobacter baumannii and the remaining 21 (5.1%) to be Acinetobacter lwoffi. Acinetobacter lwoffii showed 100% sensitivity to all the drugs. Of the 393 Acinetobacter baumannii isolates 109 (27.7%) showed resistant to Imipenem. Out of these 109 isolates, 65 (59.63%) were positive for metallo-β-lactamase production by double disk synergy test. The speciation is highly demanding and laborious but it’s important to be demonstrated due to difference in the antibiotic susceptibility pattern. Carbapenem resistant Acinetobacter nosocomial strains in ICUs are detected to be more resistant to antibiotics. As shown in this study the metallo-β-lactamase producing A.baumannii isolates were 59.63% and therapeutic options were limited. Therefore early identification of metallo-β-lactamase producers is of great importance to start appropriate treatment and to control the spread.

scholarly journals Identification and Antimicrobial Susceptibility Pattern of Clinically Significant Coagulase-negative Staphylococci

2014 ◽  
Vol 9 (2) ◽  
pp. 73-78
Author(s):  
A Nazir ◽  
A Shah ◽  
D Kakru ◽  
S M Kadri
Keyword(s):  
Antimicrobial Susceptibility ◽  
Methicillin Resistance ◽  
Antibiotic Sensitivity ◽  
Clinical Samples ◽  
Medical Sciences ◽  
Coagulase Negative Staphylococci ◽  
Antimicrobial Susceptibility Pattern ◽  
Sensitivity Pattern ◽  
Clinically Significant ◽  
A Prospective Study

Aims: This study was undertaken to determine the prevalence and pathogenicity of coagulase-negative staphylococci in clinical samples and to study the antibiotic-sensitivity pattern of the coagulase-negative isolates. Methods: A prospective study was conducted over a period of two years on patients admitted in the Sher-i-Kashmir Institute of Medical Sciences. Blood, urine, pus, catheter tips, cerebrospinal fluid and peritoneal fluid samples of patients who fulfilled the criteria for being labeled as nosocomial were cultured. Results: One hundred and six strains of coagulase negative staphylococci were isolated from the samples and among them 90 isolates were identified as Staphylococcus epidermidis (84.90%). Most of the coagulase-negative staphylococci isolates were resistant to penicillin, cephalosporins and fluoroquinolones. Methicillin resistance was found in a significant number of coagulase-negative isolates. All the isolates were found to be sensitive to vancomycin. Conclusions:  The results of the study emphasized that isolation of coagulase-negative staphylococci should be viewed with serious concern and accurate species identification and antimicrobial susceptibility testing should be performed in all cases. DOI: http://dx.doi.org/10.3126/njog.v9i2.11769

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