scholarly journals European Impatiens species differences at RAPD and ISSR loci

Biologija ◽  
2016 ◽  
Vol 62 (1) ◽  
Author(s):  
Edita Ramonienė ◽  
Lina Jocienė ◽  
Algimantas Paulauskas ◽  
Eugenija Kupčinskienė
Keyword(s):  
Genetic Differentiation ◽  
Species Differences ◽  
Geographic Area ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Genetic Distances ◽  
Molecular Characteristics ◽  
Randomly Amplified Polymorphic Dna ◽  
Rapd And Issr ◽  
Simple Sequence

Information concerning comparison of three widely spread European species of Impatiens along wider geographical areas is still missing. The present study is aimed at comparing genetic variability at RAPD and ISSR loci of Impatiens noli-tangere, I. parviflora, and I. glandulifera, covering a marked geographic area. Twenty four populations of these Impatiens (eight populations of each species) from two countries (Lithuania and Czech Republic) were examined. Eight randomly amplified polymorphic DNA (RAPD) and 5 inter simple sequence repeat (ISSR) markers were chosen considering the lack of data on the general molecular characteristics of Impatiens. The highest genetic differentiation at RAPD loci (GST = 0.81) was characteristic of I. parviflora, and the highest genetic differentiation at ISSR loci (GST = 0.73) was documented for I. glandulifera. According to Nei’s genetic distances between two species populations, significant correlations were determined for I. noli-tangere and I. parviflora (r = 0.79; p 

scholarly journals A comparative analysis of genetic diversity in Portuguese grape germplasm from ampelographic collections fit for quality wine production

2016 ◽  
Vol 14 (4) ◽  
pp. e0712 ◽  
Author(s):  
Isaura Castro ◽  
Olinda Pinto-Carnide ◽  
Jesús M. Ortiz ◽  
Vanessa Ferreira ◽  
Juan P. Martín
Keyword(s):  
Genetic Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Morphological Characters ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Molecular Techniques ◽  
Wine Production ◽  
Rapd And Issr ◽  
Simple Sequence

Grapevine cultivars diversity is vast and full of synonyms and homonyms. Up to few decades ago characterization of grapevine was based on morphological characters. In the last decades, molecular markers were developed and have been used as tools to study genetic diversity in a range of different plant species. Fifty-six Portuguese accessions representative of ‘Vinhos Verdes’ and ‘Douro’ Controlled Designations of Origin (DOC) were analysed through DNA fingerprints generated by Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR). The study aimed to compare the effectiveness of RAPD and ISSR molecular techniques in the detection of synonyms, homonyms and misnames. RAPD and ISSR analysis enabled the detection of 36 different band patterns, reducing in about 36% the initial material. Several accessions grown under different names, between and within collections, were confirmed as the same genotype, namely Gouveio/Verdelho, Sousão Douro/Vinhão and Arinto Oeste/Pedernã. Similarly, some homonyms/misnames were also identified, namely within Azal Tinto and Rabigato accessions. RAPD and ISSR markers revealed to be adequate molecular techniques for grapevine varieties fingerprinting with advantages over other molecular procedures, contributing for a good management of grapevine collections.

scholarly journals Determination of genetic diversity among some almond accessions

Genetika ◽  
2015 ◽  
Vol 47 (1) ◽  
pp. 13-22 ◽  
Author(s):  
Hasan Pinar ◽  
Sezai Ercisli ◽  
Mustafa Unlu ◽  
Mustafa Bircan ◽  
Aydın Uzun ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cultivar Identification ◽  
Genetic Relationships ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Geographical Isolation ◽  
Fruit Species ◽  
Rapd And Issr ◽  
Simple Sequence

More recently the use of different molecular markers in fruit species to determine particularly genetic diversity, genetic relationships and cultivar identification has been gained more importance. In the study, 13 randomly amplified polimorfic DNA (RAPD) and 4 inter-simple sequence repeat (ISSR) markers were used to evaluate genetic relationships among 95 almong accessions (26 foreign cultivars and 69 national cultivars and selections). The all plant material found in Almond Germplasm Repository in Gaziantep, Turkey. Both RAPD and ISSR markers distinguished the almond cultivars and selections in various levels. 17 RAPD and ISSR markers yielded a total of 73 scorable bands, which 51 are polymorphic. The two marker system exhibited variation with regard to average band sizes and polymorphism ratio. The average polymorphism was higher in ISSR (88%) compared to RAPD (74%). RAPD and ISSR marker systems were found to be useful for determining genetic diversity among almong genotypes and cultivars. Combining of two dendrograms obtained through these markers show different clustering of 96 almond specimens without geographical isolation. These results supported that almonds in Turkey indicated considerable genetic diversity.

Molecular Comparison between the Sicilian-Sardinian Sheep Populations in Tunisia using ISSR Markers

2020 ◽  
Author(s):  
H. El-Hentati ◽  
R. Aloulou ◽  
W. Derouich ◽  
O. Gaddeh
Keyword(s):  
Gene Diversity ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Genetic Distances ◽  
Shannon Index ◽  
Forage Production ◽  
Dairy Sheep ◽  
The North ◽  
Molecular Comparison ◽  
Simple Sequence

Inter simple sequence repeat (ISSR) amplification was used to study the genetic diversity between three populations of the Sicilian-Sardinian dairy sheep breed. This breed is only found in the north of Tunisia (Beja and Bizerte governorates) where the climate is subhumid (> 600 mm / year) and is favorable to large forage production. The studied animals belong to three regions: Gnadil (Beja), Nagachia (Beja) and Fretissa (Bizerte). In total, 153 bands were amplified and all were polymorphic (100%). Within populations, the Nei’s gene diversity, the Shannon index and the percentage of polymorphic loci were between 0.08 and 0.16, 0.13 and 0.27 and 30.72% and 77.78% respectively. The coefficient of gene differentiation (Gst) and the gene flow (Nm) between populations varied from 0.12-0.2 and 1.99-3.65. The UPGMA dendrogram, grouping the three studied populations, based on the Nei’s standard genetic distances showed that the populations of Nagachia and Fretissa are genetically the closest while the population of Gnadil is the most distant one.

Assessment of genetic variability in populations of Nacobbus aberrans (Thorne, 1935) Thorne & Allen, 1944 (Nematoda: Pratylenchidae) from Argentina

Nematology ◽  
2007 ◽  
Vol 9 (2) ◽  
pp. 261-270 ◽  
Author(s):  
Marcelo Doucet ◽  
Juan Rondan Dueñas ◽  
Paola Lax ◽  
Cristina Gardenal
Keyword(s):  
Genetic Differentiation ◽  
Anthropogenic Activities ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Severe Damage ◽  
Second Stage ◽  
Nacobbus Aberrans ◽  
Low Levels ◽  
Simple Sequence ◽  
Natural Means

AbstractNacobbus aberrans produces severe damage to agriculture and is considered to be of quarantine importance. The levels of polymorphism and genetic differentiation among Argentine populations of this species from different hosts and origin were studied using Inter-Simple Sequence Repeat (ISSR) as molecular markers. Second-stage juveniles from nine populations associated with three different hosts (tomato, quinoa and potato) were analysed individually. Three primers were selected because they produced clear and 100% reproducible patterns; 37 fragments were considered for the analysis. The technique employed revealed high levels of polymorphisms, the highest proportion of genetic diversity being found within populations (95%). Populations showed a tendency to group together according to their original host, based on their pairwise FST. Low levels of genetic differentiation among populations were observed, suggesting an extensive gene flow among them. Passive dispersal of nematodes by natural means and anthropogenic activities would probably be, at least partially, responsible for the results observed. This work is the first study of genetic structure of N. aberrans populations at a macrogeographical level using ISSR markers.

scholarly journals RAPD and ISSR Methods Used for Fingerprinting of Selected Accessions of Viburnum

2014 ◽  
Vol 6 (3) ◽  
pp. 292-299
Author(s):  
Marcelina KRUPA-MAŁKIEWICZ ◽  
Miłosz Smolik ◽  
Anna BARNIAK ◽  
Beata SMOLIK
Keyword(s):  
Genetic Variability ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Mantel Test ◽  
North West ◽  
The North ◽  
West Part ◽  
Rapd And Issr ◽  
Simple Sequence ◽  
Issr Primers

Randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers were used to investigate genetic variability within thirteen Viburnum species (Viburnum × hillieri; V. dilatatum; Viburnum × carlcephalum; V. opulus; V. hupehense; Viburnum× bodnantense; Viburnum × burkwoodii; V. sieboldii; Viburnum × globosum ‘Jermyns Globe’; V. alnifolium (lantanoides); V. plicatum ‘Sterile’; V. plicatum f. tomentosum and V. plicatum ‘Watanabe’) of wide geographical distribution, collected in the Dendrological Garden in Przelewice (the north-west part of Poland). Twenty-three RAPD and fourteen ISSR primers generated a total of 690 and 418 reproducible bands, respectively, and 39% (RAPD) and 55.5% (ISSR) of them were polymorphic for the two marker systems, which suggest high genetic variability within Viburnum genus. However, high numbers of genotype-specific bands, i.e. 60.9% (RAPD) and 44.5% (ISSR), were seen in Viburnum. Genetic similarity assessed within Viburnum species with the RAPD and ISSR analyses ranged from 6 to 42% and from 6 to 31%, respectively. Both RAPD and ISSR-based dendrograms clustered in five main groups. The Mantel test between two Nei’s similarity matrices gave correlation coefficient r=0.305*, showing low correlation between RAPD- and ISSR- based matrices. Thus, both marker systems were equally important for the genetic diversity analysis in Viburnum genus.

scholarly journals Population Structure and Genetic Diversity of the Medicinal and Aromatic Plant Cunila spicata Benth. (Lamiaceae) Based on Inter-Simple Sequence Repeat (ISSR) Markers

2015 ◽  
Vol 5 (2) ◽  
pp. 652-658
Author(s):  
Ana Paula Longaray Delamare ◽  
Sergio Echeverrigaray ◽  
Marcos Albuquerque ◽  
Jucimar Zacaria
Keyword(s):  
Genetic Diversity ◽  
Population Level ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Genetic Distances ◽  
Aromatic Plant ◽  
Low Genetic Diversity ◽  
Main Factors ◽  
Simple Sequence ◽  
South Brazil

Cunila spicata is an endangered aromatic and medicinal plant of South Brazil. In the present paper, the ISSR technique was employed to study the intra- and inter-population genetic diversity of this species. Nine primers generated a total of 109 amplification products, most of which were polymorphics. Low genetic diversity at population level (HE= 0.053) and species level (HT=0.196), and high differentiation among populations (GST= 0.727) were detected in C. spicata. The genetic diversity, low estimated genetic flow and absence of correlation between genetic distances, geographic distances and chemical composition, indicates that genetic drift and inbreeding may be the main factors involved in the genetic structure of C. spicata. Based on these findings, strategies are proposed for the genetic conservation and management of this species. 

scholarly journals Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers

2020 ◽  
Vol 42 ◽  
pp. e53606
Author(s):  
Gizele de Andrade Luz ◽  
Jailson de Araújo Santos ◽  
Kelly Pires de Oliveira ◽  
Pâmela Ponce Martins ◽  
Sérgio Emílio dos Santos Valente ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Differentiation ◽  
Genetic Erosion ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Genetic Groups ◽  
Distrito Federal ◽  
The Mean ◽  
Hancornia Speciosa ◽  
Simple Sequence

 The mangabeira is a native fruit tree from Brazil with fruits that present significant potential for exploitation. This species is experiencing genetic erosion, which increases the importance of elucidating the genetic diversity that exists in mangabeira populations to support conservation programs. Thus, this study aimed to evaluate the diversity and genetic structure of mangabeira populations from the Germplasm Bank of Embrapa Meio-Norte using inter simple sequence repeat (ISSR) markers. A total of 29 accessions from Brazil were characterized, including one from Sergipe, one from Bahia, three from the Distrito Federal, 11 from Piauí and 13 from Paraíba. The 11 ISSR primers provided 166 loci, among which 120 were polymorphic. The analysis of molecular variance (AMOVA) indicated that 69.66% of the observed genetic variability occurred within populations and that the populations showed high genetic differentiation. The results obtained from the STRUCTURE analysis indicated the existence of two genetic groups. The Nei and Shannon indices of genetic diversity varied from 0.15 to 0.24 and from 0.22 to 0.34, respectively. The coefficient of similarity ranged from 0.57 to 0.94, with a mean of 0.76. The mean was used as the cut-off point in the dendrogram, and seven groups were identified. In conclusion, this study demonstrates the presence of low or moderate genetic diversity within the studied mangabeira populations and high genetic differentiation between the populations. The results indicate a need to increase the number of mangabeira population samples from different collection sites as a strategy to achieve more significant results for the conservation and genetic improvement of this species.

Species-diagnostic and species-specific DNA sequences evenly distributed throughout pine and spruce chromosomes

Genome ◽  
2010 ◽  
Vol 53 (10) ◽  
pp. 769-777 ◽  
Author(s):  
Melanie Mehes-Smith ◽  
Paul Michael ◽  
Kabwe Nkongolo
Keyword(s):  
Dna Sequences ◽  
Random Amplified Polymorphic Dna ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Pinus Monticola ◽  
The Family ◽  
Species Specific ◽  
Rapd And Issr ◽  
Specific Sequences

Genome organization in the family Pinaceae is complex and largely unknown. The main purpose of the present study was to develop and physically map species-diagnostic and species-specific molecular markers in pine and spruce. Five RAPD (random amplified polymorphic DNA) and one ISSR (inter-simple sequence repeat) species-diagnostic or species-specific markers for Picea mariana , Picea rubens , Pinus strobus , or Pinus monticola were identified, cloned, and sequenced. In situ hybridization of these sequences to spruce and pine chromosomes showed the sequences to be present in high copy number and evenly distributed throughout the genome. The analysis of centromeric and telomeric regions revealed the absence of significant clustering of species-diagnostic and species-specific sequences in all the chromosomes of the four species studied. Both RAPD and ISSR markers showed similar patterns.

ISSR Marker Based Genetic Diversity in Morinda Spp. For Its Enhanced Collection, Conservation and Utilization

2021 ◽  
Author(s):  
Lalit Arya ◽  
Ramya Kossery Narayanan ◽  
Anjali Kak ◽  
Chitra Devi Pandey ◽  
Manjusha Verma ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Gene Diversity ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Morinda Citrifolia ◽  
Species Differentiation ◽  
Information Index ◽  
Upgma Cluster Analysis ◽  
Simple Sequence

Abstract Morinda (Rubiaceae) is considerably recognized for its multiple uses viz. food, medicine, dyes, firewood, tools, oil, bio-sorbent etc. The molecular characterization of such an important plant would be very useful for its multifarious enhanced utilization. In the present study, 31 Morinda genotypes belonging to two different species Morinda citrifolia and Morinda tomentosa collected from different regions of India were investigated using Inter Simple Sequence Repeat (ISSR) markers. Fifteen ISSR primers generated 176 bands with an average of 11.7 bands per primer, of which (90.34%) were polymorphic. The percentage of polymorphic bands, mean Nei’s gene diversity, mean Shannon’s information index in Morinda tomentosa and Morinda citrifolia was [(69.89%, 30.68%); (0.21 ± 0.19, 0.12 ± 0.20); (0.32 ± 0.27 0.17 ± 0.28)] respectively, revealing higher polymorphism and genetic diversity in Morinda tomentosa compared to Morinda citrifolia. Structure, and UPGMA cluster analysis placed the genotypes into well-defined separate clusters belonging to two species Morinda tomentosa and Morinda citrifolia revealing the utility of ISSR markers in species differentiation. Distinct ecotypes within a particular species could also be inferred emphasizing the collection and conservation of Morinda genotypes from different regions, in order to capture the overall diversity of respective species. Further higher diversity of M. tomentosa must be advanced for its utilization in nutraceutical, nutritional and other nonfood purposes.

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