scholarly journals Antifungal activity of paracloacal gland secretion of Caiman yacare (DAUDIN, 1802)

2018 ◽  
Vol 9 (1) ◽  
pp. 140-151
Author(s):  
Lucas Polizzeli Azevedo ◽  
Leandro Nogueira Pressinotti ◽  
Rhavena Graziela Liotti ◽  
Carla Galbiati ◽  
Elaine Maria Loureiro ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Ethanolic Extract ◽  
Minimum Fungicidal Concentration ◽  
Sabouraud Agar ◽  
Local Product ◽  
In Series ◽  
Pharmacological Potential ◽  
Resazurin Dye ◽  
In Vitro Antifungal Activity

The objective of this study was to evaluate the in vitro antifungal activity of ethanolic and aqueous extracts obtained from Caiman yacare paracloacal gland (PG) against Candida albicans (ATCC 10231), as well as a bibliographical survey on the chemical composition of exudates of PG. The PG were collected from the disposal generated during the slaughter of C. yacare by regularized industry. Two extracts were made from these glands, one ethanolic and the other aqueous. The Minimal Inhibitory Concentration (MIC) of the substances were determined by dilution of the extract in series using the microdilution technique in the culture medium Sabouraud broth, carried out in a 96-well microplate visually read after 48 hours of incubation, confirmed by the method using 0.01% aqueous resazurin dye. The ethanolic extract had MIC at the concentration of 25 µg / L. The Minimum Fungicidal Concentration (MFC) was determined by subcultures of MIC in Sabouraud agar medium. The ethanolic extract presented MFC at a concentration of 50 µg / mL. The aqueous extract showed no antifungal activity at the concentrations tested. This work is the first work to assess an activity of the PG secretion and reveals pharmacological potential in a local product previously discarded.

scholarly journals IN VITRO ANTIFUNGAL ACTIVITY OF TIMOL AND CARVACROL ON CANDIDA SPECIES

2019 ◽  
Vol 4 ◽  
Author(s):  
Luciana Thaís Rangel Souza ◽  
Cecília Correia Costa ◽  
Mateus Cardoso Oliveira ◽  
Isabel Celeste Caires Pereira Gusmão
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Inhibitory Concentration ◽  
Candida Krusei ◽  
Minimum Fungicidal Concentration ◽  
Sabouraud Agar ◽  
In Vitro Antifungal Activity

Aim: to evaluate the in vitro action of thymol and carvacrol against the yeasts of Candida albicans ATCC10231 and Candida krusei ATCC34135. Method: A laboratory study was performed to evaluate antifungal activity. The characterization of the Minimal Inhibitory Concentration (MIC) of the thymol essential oil was carried out using the technique where the microdilution is performed, in which a plate containing 96 wells is used. The determination of the Minimum Fungicidal Concentration (MFC) was performed by dripping 10 μL of each of the concentrations evaluated on Sabouraud agar plates. Results: The MIC of thymol and carvacrol for C. albicans was 40 μg/mL and for Candida krusei it did not present antifungal activity. While the MIC of nystatin was 0.03mg for both species with thymol and carvacrol. Conclusion: Thymol presented satisfactory antifungal activity against the pathogens studied, but carvacrol did not present antifungal activity.

scholarly journals 7-Chloroquinolin-4-yl Arylhydrazone Derivatives: Synthesis and Antifungal Activity

2011 ◽  
Vol 11 ◽  
pp. 1489-1495 ◽  
Author(s):  
Auri R. Duval ◽  
Pedro H. Carvalho ◽  
Maieli C. Soares ◽  
Daniela P. Gouvêa ◽  
Geonir M. Siqueira ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Rational Design ◽  
Antifungal Agents ◽  
Inhibitory Concentration ◽  
First Line ◽  
Minimum Fungicidal Concentration ◽  
Starting Point ◽  
Line Drug ◽  
In Vitro Antifungal Activity

Fifteen 7-chloro-4-arylhydrazonequinolines have been evaluated for their in vitro antifungal activity against eight oral fungi:Candida albicans, C. parapsilosis, C. lipolytica, C. tropicalis, C. famata, C. glabrata, Rhodutorula mucilaginosa, andR. glutinis. Several compounds exhibited minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) activities comparable with the first-line drug fluconazole. These results could be considered as an important starting point for the rational design of new antifungal agents.

scholarly journals Antifungal Activity of Plumericin and Isoplumericin

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Dharmendra Singh ◽  
Umakant Sharma ◽  
Parveen Kumar ◽  
Yogesh K Gupta ◽  
M. P. Dobhal ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Cryptococcus Neoformans ◽  
Inhibitory Concentration ◽  
Chloroform Extract ◽  
Drug Candidate ◽  
Minimum Fungicidal Concentration ◽  
In Vitro Antifungal Activity

This study evaluated the in vitro antifungal activity of the chloroform extract of Plumeria bicolor and its phytoconstituents plumericin and isoplumericin against Candida species and Cryptococcus neoformans by measuring the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC). Plumericin's consistently high activity against Candida albicans, C. krusei, C. glabrata, C. tropicalis and Cryptococcus neoformans was more potent than isoplumericin and the standard antifungal drug nystatin suggesting its potential as a drug candidate for candidiasis and cryptococcosis.

scholarly journals Antifungal Activity of the Essential Oils from Ocimum gratissimum L. Grown in Togo

2008 ◽  
Vol 1 (1) ◽  
pp. 164-171 ◽  
Author(s):  
Koffi Koba ◽  
P W Poutouli ◽  
Christine Raynaud ◽  
Komla Sanda
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Filamentous Fungi ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Minimum Fungicidal Concentration ◽  
Ocimum Gratissimum ◽  
Aerial Parts ◽  
In Vitro Antifungal Activity

The aerial parts of Ocimum gratissimum L. (Lamiaceae) harvested in Togo was steam-distilled and investigated for essential oil composition (GC and GC/MS) and in vitro antifungal activities. Thymol (31.79 %), p-cymene (15.57 %) and γ-terpinene (12.34 %) and were the major components of the oil. Other notable components identified in this oil were myrcene (6.94 %) and α-thujene (6.11 %).The in vitro antifungal activity was recorded with the minimum inhibitory concentrations (MICs) ranging from 80 to 150 µl.l-1, 150 to 500 µl.l-1  and from 100 to 150 µl.l-1 respectively on dermatophytes, imperfect filamentous fungi and pathogenic yeasts. Likewise, on tested fungi the minimum fungicidal concentration (MFC) varied from 300 µl.l-1 to 500 µl.l-1, 500 to 700 µl.l-1 and from 250 to 300 µl.l-1, respectively on dermatophytes, imperfect filamentous fungi and pathogenic yeasts. Keywords: O.gratissimum,  Antifungal, Essential oil; Thymol. © 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v1i1.1131 

In vitro antifungal activity of some Mannich bases of conjugated styryl ketones

1998 ◽  
Vol 44 (1) ◽  
pp. 74-79 ◽  
Author(s):  
Elias K Manavathu ◽  
S C Vashishtha ◽  
George J Alangaden ◽  
Jonathan R Dimmock
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Fungal Growth ◽  
Mannich Bases ◽  
Minimum Fungicidal Concentration ◽  
Dependent Inhibition ◽  
Normal Human ◽  
Further Development ◽  
In Vitro Antifungal Activity

Four Mannich bases of some conjugated styryl ketones IIa-IId were examined for antifungal activity. These compounds were designed as thiol-alkylators and had two centers for attack by cellular thiols. The most potent compounds IIa and IIb possessed hydrophobic, electron-attracting substituents in the aryl rings and in general had minimum inhibitory concentration (MIC) values of 0.2-25 μM against a variety of fungi. None of the four compounds inhibited the growth of a number of bacteria (MIC > 100 μM). The minimum fungicidal concentration (MFC) values for IIa and IIb were generally either similar or twofold higher than the MIC figures for fungi. Compound IIa demonstrated rapid, concentration-dependent inhibition of the growth of Candida albicans B311. The toxicity of IIa to normal human cells was much lower than the concentrations of this compound required to inhibit fungal growth. In summary, this study of four prototypic molecules has revealed that this class of compounds may have potential for further development as candidate antifungal agents.Key words: antifungal, styryl ketones, Candida albicans, Aspergillus fumigatus, susceptibility test.

scholarly journals In vitro antifungal activity of three Saudi plant extracts against some Phytopathogenic fungi

2012 ◽  
Vol 52 (4) ◽  
pp. 458-462 ◽  
Author(s):  
Abdul Aziz A. Al-Askar
Keyword(s):  
Antifungal Activity ◽  
Plant Extracts ◽  
Sclerotium Rolfsii ◽  
Ethanolic Extract ◽  
Punica Granatum ◽  
Phytopathogenic Fungi ◽  
Plant Diseases ◽  
High Concentration ◽  
In Vitro Antifungal Activity

Abstract The antifungal activities of ethanolic extracts of three Saudi plants; camel thorn (Alhagi maurorum Medic.), caper (Capparisspinosa L.), and pomegranate (Punica granatum L.) were investigated in vitro against Alternaria alternata, Fusarium oxysporum, Phomadestructiva, Rhizoctonia solani, and Sclerotium rolfsii at concentrations of 0, 3, 6, and 9% (v/v). All tested plant extracts; seeds, roots, and rinds had different degrees of antifungal activity against the tested fungi. When compared with the control, the highest antifungal activity was recorded for camel thorn seeds extract at a concentration of 9%, while, pomegranate rinds extract at 9% came in second. Camel thorn rinds extract came in last even when used at a high concentration. The ethanolic extract of camel thorn seeds may be recommended as a potent bio-fungicide. Extensive studies should be undertaken for the ethanolic extract of camel thorn seeds as a strong antifungal agent against fungal plant diseases.

scholarly journals THE STUDY OF ANTIFUNGAL ACTIVITY FROM INDIGENOUS PLANTS FROM INDONESIA: AN IN VITRO STUDY

2016 ◽  
Vol 10 (1) ◽  
pp. 196
Author(s):  
Dewi Safitri ◽  
Elin Yulinah Sukandar ◽  
Irda Fidrianny ◽  
Eriwan Susanto
Keyword(s):  
Antifungal Activity ◽  
In Vitro Study ◽  
Ethanolic Extract ◽  
Trichophyton Mentagrophytes ◽  
Rosmarinus Officinalis ◽  
Microsporum Gypseum ◽  
Indigenous Plants ◽  
Reference Drug ◽  
Minimum Fungicidal Concentration

Objective: This study aimed to look for new agents from extracted Indonesia herbs which have antifungal activity with better safety profile against Candida albicans, Microsporum gypseum, and Trichophyton mentagrophytes. Methods: Screening of eleven herbs was perform to determine the highest antifungal activity. Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) of selected extracts, rosemary (Rosmarinus officinalis) leaves, Java turmeric (Curcuma xanthorriza) rhizome, and ginger (Zingiber officinalis) rhizome; were determined by using microdilution and agar diffusion methods. Extracts were then combined to evaluate further activities. Selected extracts were fractionated by using liquid-liquid extraction, analyzed by TLC bioautography.Results: ethanolic extract of rosemary (Rosmarinus officinalis) leaves, Java turmeric (Curcuma xanthorriza) rhizome, and ginger (Zingiber officinalis) rhizome in combination showed the highest activity and synergistic interaction against Trichophyton mentagrophytes. There were several components from fractions were actively inhibiting corresponding fungi according to TLC bioautography method. Conclusion: ethanolic extracts of rosemary, Java turmeric, and ginger had the highest antifungal potency, both as extract and in combination comparable to ketoconazole as reference drug. These selected extracts are potential to be used as new antifungal agents.

In vitro Antifungal Activity of a 29-kDa Glycoprotein Purified from the Galls of Quercus infectoria

2005 ◽  
Vol 40 (1-2) ◽  
pp. 43-54 ◽  
Author(s):  
K. Yamunarani ◽  
R. Jaganathan ◽  
R. Bhaskaran ◽  
P. Govindaraju ◽  
R. Velazhahan
Keyword(s):  
Antifungal Activity ◽  
Quercus Infectoria ◽  
In Vitro Antifungal Activity

Formulation and Evaluation of Carbopol Gel Containing Liposomes of Ketoconazole. (Part-II)

2009 ◽  
Vol 1 (2) ◽  
Author(s):  
Rakesh Patel ◽  
Hardik Patel ◽  
Ashok Baria
Keyword(s):  
Antifungal Activity ◽  
Rat Skin ◽  
Albino Rat ◽  
In Vitro Drug Release ◽  
Carbopol Gel ◽  
Liposomal Formulations ◽  
Release Drug ◽  
In Vitro Permeation ◽  
In Vitro Antifungal Activity

The aim of this work was to prepare and evaluate the topical carbopol gel formulation containing ketoconazole encapsulated liposomes. Ketoconazole loaded liposomes were prepared by thin film hydration technique. The prepared liposomes were incorporated into 1% carbopol gel, and the systems were evaluated for in-vitro drug release, drug retention into skin and in-vitro antifungal activity. The in-vitro permeation of ketoconazole using wistar albino rat skin from liposomal gel was compared with that of plain drug gel and also with plain drug cream containing 2% w/w of ketoconazole. The release of ketoconazole from liposomal gel was much slower than from non liposomal formulations. Gel containing liposomal ketoconazole showed maximum antifungal activity after 30 hours over plain ketoconazole gel and cream formulations.

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