scholarly journals Monitoring innate immune cell dynamics in the glioma microenvironment by magnetic resonance imaging and multiphoton microscopy (MR-MPM)

Theranostics ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 1873-1883 ◽  
Author(s):  
Kianush Karimian-Jazi ◽  
Philipp Münch ◽  
Allen Alexander ◽  
Manuel Fischer ◽  
Kira Pfleiderer ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Immune Cell ◽  
Multiphoton Microscopy ◽  
Innate Immune ◽  
Innate Immune Cell ◽  
Resonance Imaging ◽  
Cell Dynamics ◽  
Glioma Microenvironment

scholarly journals Multiscale structural analysis of mouse lingual myoarchitecture employing diffusion spectrum magnetic resonance imaging and multiphoton microscopy

2008 ◽  
Vol 13 (6) ◽  
pp. 064005 ◽  
Author(s):  
Terry A. Gaige ◽  
Hyuk Sang Kwon ◽  
Guangping Dai ◽  
Victor C. Cabral ◽  
Ruopeng Wang ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Structural Analysis ◽  
Magnetic Resonance ◽  
Multiphoton Microscopy ◽  
Resonance Imaging

scholarly journals Characterization of Transverse Aortic Constriction in Mice Based on the Specific Recruitment of Leukocytes to the Hypertrophic Myocardium and the Aorta Ascendens

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Jan Lukas Kleiner ◽  
Odilia Köpke ◽  
Anton Faron ◽  
Yunyang Zhang ◽  
Jan Cornelssen ◽  
...  
Keyword(s):  
Immune Response ◽  
High Pressure ◽  
Immune Cell ◽  
Left Ventricular ◽  
Innate Immune ◽  
Innate Immune Cell ◽  
Transverse Aortic Constriction ◽  
Aortic Constriction ◽  
Cell Dynamics ◽  
Aorta Ascendens

Transverse aortic constriction (TAC) is a model that mimics pressure overload-induced left ventricular (LV) hypertrophy in mice. Alterations in immune cell functionality can promote cardiac and vascular remodeling. In the present study, we characterized the time course in innate immune cell dynamics in response to TAC in the different tissues of mice. It was determined whether TAC induces a characteristic leukocyte-driven immune response in the myocardium, aorta ascendens and descendens, spleen, blood, and draining lymph nodes supported by cytokine-driven chemotaxis in mice at 3, 6, and 21 days following surgery. We used complex flow cytometry staining combinations to characterize the various innate immune cell subsets and a multiplex array to determine cytokine concentrations in the serum. The results of the current study indicated that leukocytes accumulate in the myocardium and aorta ascendens in response to TAC. The leukocyte dynamics in the myocardium were dominated by the Ly6Clow macrophages with an early accumulation, whereas the response in the aorta ascendens was characterized by a long-lasting proinflammatory phenotype driven by Ly6Chigh macrophages, neutrophils, and activated DCs. In contrast to the high-pressure environment of the aorta ascendens, the tissue of the aorta descendens did not react to TAC with any leukocyte increase. The levels of proinflammatory cytokines in the blood were elevated in response to TAC, indicating a systemic reaction. Moreover, our findings strongly suggest that cardiac macrophages could origin from splenic pools and reach the site of the inflammation via the blood. Based on the current findings, it can be concluded that the high-pressure conditions in the aorta ascendens cause a characteristic immune response, dominated by the accumulation of leukocytes and the activation of DCs that varies in comparison to the immune cell dynamics in the myocardium and the aorta descendens.

scholarly journals The Microarchitecture of Pancreatic Cancer as Measured by Diffusion-Weighted Magnetic Resonance Imaging Is Altered by T Cells with a Tumor Promoting Th17 Phenotype

2020 ◽  
Vol 21 (1) ◽  
pp. 346 ◽  
Author(s):  
Philipp Mayer ◽  
Alica Linnebacher ◽  
Hannah Glennemeier-Marke ◽  
Nicole Marnet ◽  
Frank Bergmann ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
T Cells ◽  
Magnetic Resonance ◽  
Cell Lines ◽  
Immune Cell ◽  
Ductal Adenocarcinoma ◽  
Resonance Imaging ◽  
Pancreatic Cell ◽  
Diffusion Weighted ◽  
Weighted Magnetic Resonance Imaging

Diffusion-weighted magnetic resonance imaging (DW-MRI) is a diagnostic tool that is increasingly used for the detection and characterization of focal masses in the abdomen, among these, pancreatic ductal adenocarcinoma (PDAC). DW-MRI reflects the microarchitecture of the tissue, and changes in diffusion, which are reflected by changes in the apparent diffusion coefficient (ADC), are mainly attributed to variations in cellular density, glandular formation, and fibrosis. When analyzing the T cell infiltrates, we found an association of a tumor-promoting subpopulation, characterized by the expression of interleukin (IL) 21 and IL26, with high ADC values. Moreover, the presence of IL21+ and IL26+ positive T cells was associated with poor prognosis. Pancreatic cancers—but not healthy pancreatic tissue—expressed receptors for IL21 and IL26, a finding that could be confirmed in pancreatic cell lines. The functionality of these receptors was demonstrated in pancreatic tumor cell lines, which showed phosphorylation of ERK1/2 and STAT3 pathways in response to the respective recombinant interleukins. Moreover, in vitro data showed an increased colony formation of tumor cells. In summary, our data showed an association of IL21+ and IL26+ immune cell infiltration, increased ADC, and aggressive tumor disease, most likely due to the activation of the key cancer signaling pathways ERK1/2 and STAT3 and formation of tumor colonies.

Functional information from magnetic resonance imaging

1995 ◽  
Vol 53 ◽  
pp. 84-85
Author(s):  
Alan P. Koretsky ◽  
Afonso Costa e Silva ◽  
Yi-Jen Lin
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
High Resolution ◽  
Cancer Biology ◽  
Imaging Modality ◽  
Magnetic Resonance Images ◽  
Great Success ◽  
Functional Information ◽  
Resonance Imaging ◽  
High Resolution Mri

Magnetic resonance imaging (MRI) has become established as an important imaging modality for the clinical management of disease. This is primarily due to the great tissue contrast inherent in magnetic resonance images of normal and diseased organs. Due to the wide availability of high field magnets and the ability to generate large and rapidly switched magnetic field gradients there is growing interest in applying high resolution MRI to obtain microscopic information. This symposium on MRI microscopy highlights new developments that are leading to increased resolution. The application of high resolution MRI to significant problems in developmental biology and cancer biology will illustrate the potential of these techniques.In combination with a growing interest in obtaining high resolution MRI there is also a growing interest in obtaining functional information from MRI. The great success of MRI in clinical applications is due to the inherent contrast obtained from different tissues leading to anatomical information.

Functional Magnetic Resonance Imaging Measures of Blood Flow Patterns in the Human Auditory Cortex in Response to Sound

1998 ◽  
Vol 41 (3) ◽  
pp. 538-548 ◽  
Author(s):  
Sean C. Huckins ◽  
Christopher W. Turner ◽  
Karen A. Doherty ◽  
Michael M. Fonte ◽  
Nikolaus M. Szeverenyi
Keyword(s):  
Magnetic Resonance Imaging ◽  
Blood Flow ◽  
Magnetic Resonance ◽  
Functional Magnetic Resonance Imaging ◽  
Functional Magnetic Resonance ◽  
Resonance Imaging ◽  
Speech Stimuli ◽  
Complex Stimuli ◽  
Scanning Session ◽  
Auditory Research

Functional Magnetic Resonance Imaging (fMRI) holds exciting potential as a research and clinical tool for exploring the human auditory system. This noninvasive technique allows the measurement of discrete changes in cerebral cortical blood flow in response to sensory stimuli, allowing determination of precise neuroanatomical locations of the underlying brain parenchymal activity. Application of fMRI in auditory research, however, has been limited. One problem is that fMRI utilizing echo-planar imaging technology (EPI) generates intense noise that could potentially affect the results of auditory experiments. Also, issues relating to the reliability of fMRI for listeners with normal hearing need to be resolved before this technique can be used to study listeners with hearing loss. This preliminary study examines the feasibility of using fMRI in auditory research by performing a simple set of experiments to test the reliability of scanning parameters that use a high resolution and high signal-to-noise ratio unlike that presently reported in the literature. We used consonant-vowel (CV) speech stimuli to investigate whether or not we could observe reproducible and consistent changes in cortical blood flow in listeners during a single scanning session, across more than one scanning session, and in more than one listener. In addition, we wanted to determine if there were differences between CV speech and nonspeech complex stimuli across listeners. Our study shows reproducibility within and across listeners for CV speech stimuli. Results were reproducible for CV speech stimuli within fMRI scanning sessions for 5 out of 9 listeners and were reproducible for 6 out of 8 listeners across fMRI scanning sessions. Results of nonspeech complex stimuli across listeners showed activity in 4 out of 9 individuals tested.

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