scholarly journals Peer Review #1 of "Chemical composition, antioxidant and antitumor activities of sub-fractions of wild and cultivated Pleurotus ferulae ethanol extracts (v0.1)"

2018 ◽  
Keyword(s):  
Chemical Composition ◽  
Peer Review ◽  
Antitumor Activities ◽  
Pleurotus Ferulae ◽  
Ethanol Extracts

scholarly journals Chemical composition, antioxidant and antitumor activities of sub-fractions of wild and cultivated Pleurotus ferulae ethanol extracts

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e6097 ◽  
Author(s):  
Yi Yang ◽  
Changshuang Fu ◽  
Fangfang Zhou ◽  
Xiaoyu Luo ◽  
Jinyu Li ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Human Gastric Cancer ◽  
Oxygen Species ◽  
Antitumor Activities ◽  
Pleurotus Ferulae ◽  
The Difference ◽  
Ethanol Extracts ◽  
Human Esophageal Cancer ◽  
Human Cervical Cancer

Pleurotus ferulae is an edible and medicinal mushroom with various bioactivities. Here, the ethanol extracts of wild and cultivated P. ferulae (PFEE-W and PFEE-C) and their subfractions including petroleum ether (Pe-W/Pe-C), ethyl acetate (Ea-W/Ea-C) and n-butanol (Ba-W/Ba-C) were prepared to evaluate their antioxidant and antitumor activities. Both PFEE-W and PFEE-C show the antioxidant activity and PFEE-W is stronger than PFEE-C. The antioxidant activities of their subfractions are in the following order: Ea > Ba > Pe. Moreover, PFEE-W and PFEE-C significantly inhibit the proliferation of murine melanoma B16 cells, human esophageal cancer Eca-109 cells, human gastric cancer BGC823 cells and human cervical cancer HeLa cells through induction of apoptosis, which partially mediated by reactive oxygen species. The antitumor activities of their subfractions are in the following order: Ea ≥ Pe > Ba. Pe-W shows higher antitumor activity compared with Pe-C, which might be correlated with the difference of their components identified by gas chromatography-mass spectrometry. These results suggest that both wild and cultivated P. ferulae have antioxidant and antitumor activities, and cultivated P. ferulae could be used to replace wild one in some functions.

CHEMICAL COMPOSITION OF VARIOUS COMMERCIAL GRADES OF CANADIAN FLUE-CURED TOBACCO

1958 ◽  
Vol 38 (1) ◽  
pp. 73-80 ◽  
Author(s):  
J. M. Elliot ◽  
E. C. Birch
Keyword(s):  
Chemical Composition ◽  
Reducing Sugars ◽  
Petroleum Ether Extract ◽  
Correlation Coefficients ◽  
Protein Nitrogen ◽  
Laboratory Methods ◽  
Total Sugars ◽  
Total Alkaloids ◽  
Positive Correlations ◽  
Ethanol Extracts

A study was made of the chemical composition of 21 commercial grades of Canadian flue-cured tobacco, selected from a 50-acre crop of Hicks variety in 1955. Arbitrary prices were assigned to the various grades of tobacco. Correlation coefficients between the chemical values and the assigned grade prices were calculated. Ethanol extracts, total sugars, reducing sugars, and hygroscopicity gave significant positive correlations; total nitrogen, protein nitrogen, total alkaloids, nicotine, calcium, and magnesium gave negative correlations. These coefficients indicated that quality measured by these laboratory methods conformed with leaf-graded quality. Correlation coefficients were not significant between grade quality and petroleum ether extract, sucrose, starch, ash, silica, potassium, phosphorus, chlorine, sulphur, burn, or pH.

scholarly journals Mangifera indica Bark Essential Oil: Chemical Composition and Biological Activities in Comparison with Aqueous and Ethanol Extracts

2018 ◽  
Vol 13 (7) ◽  
pp. 1934578X1801300
Author(s):  
Gislaine Aurelie Kemegne ◽  
Maximilienne Ascension Nyegue ◽  
Sylvain Leroy Sado Kamdem ◽  
François-Xavier Etoa ◽  
Chantal Menut
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Mangifera Indica ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Scavenging Activity ◽  
Total Phenols ◽  
Dpph Method ◽  
Ethanol Extracts

Ethnobotanic surveys have revealed the use of Mangifera indica L. (Anacardiaceae) bark for the treatment of infectious diarrhea. The essential oil of M. indica bark is described for the first time for its chemical composition, radical scavenging activity (DPPH method) and antimicrobial properties. The total phenols content of its water and ethanol bark extracts as well as their radical scavenging and antimicrobial properties were also evaluated. Four commercial plant extracts were also studied for a comparison purpose. The antimicrobial activities were measured for all samples against three Gram (-): Escherichia coli, Salmonella enteritidis, Shigella and two Gram (+): Staphylococcus aureus and Bacillus cereus bacteria. The M. indica bark essential oil was characterized by the association of two major sesquiterpenes: ( E)-β-caryophyllene (60.3%) and α-humulene (36.7%). It presented the lowest ratio of concentration to inhibition zone diameter on all the strains. The aqueous and ethanol extracts of M. indica bark were characterized by high contents of total phenols compounds and high radical scavenging activity compared to the essential oil. Finally, the interesting combination of the antibacterial and antiradical activities of the aqueous M. indica bark extract justifies the traditional use of this plant part in decoction form for the treatment of diarrheal infections.

Comparative study of the ethanol extracts of six Acinos Miller species: chemical composition, antimicrobial and antioxidative activities

2020 ◽  
Vol 48 (1) ◽  
pp. 53-65
Author(s):  
Tatjana D. GOLUBOVIĆ ◽  
Gordana S. STOJANOVIĆ ◽  
Dušanka V. KITIĆ ◽  
Bojan K. ZLATKOVIĆ ◽  
Dragana R. PAVLOVIĆ ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Salmonella Enteritidis ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Fungal Organisms ◽  
Ethanol Extracts

The ethanol extracts of selected Acinos Miller species were investigated in terms of chemical composition, antimicrobial and antioxidative activities. Qualitative and quantitative analyses of the extracts were performed using GC and GC-MS. Eighty-six constituents, accounting for 93.70-99.99% of the total composition of the extracts, were identified. The ethanol extracts of A. majoranifolius, A. suaveolens and A. alpinus were characterized by domination of monoterpenes, representing 85.03%, 57.39% and 28.02% of the total extracts, respectively. Fatty acids and their esters in the A. arvensis and A. graveolens extracts reached 28.97% and 30.75%. Also, n-alkanes were the major compounds found in A. hungaricus ethanol extract (30.98%). The extracts were characterized by determination of total polyphenols, flavonoids and tannins. Besides, the antioxidant activity of the investigated extracts was estimated by two assays: DPPH and FRAP test. The highest antioxidant activity was observed in the extract of A. alpinus which had high levels of all examined polyphenol classes. A disk diffusion method was employed for the determination of the antimicrobial activities of the ethanol extracts. Gram-positive: Bacillus subtilis, Sarcina lutea, Staphylococcus aureus, Clostridium pyogenes, Enterococcus sp., Micrococcus flavus; Gram-negative: Klebsiella pneumoniae, Pseudomonas aerugionsa, Salmonella enteritidis, Proteus vulgaris, Escherichia coli and fungal organisms: Aspergillus niger, Candida albicans and Saccharomyces cerevisiae were used as test microorganisms. The results of preliminary bioassay demonstrated that the A. alpinus ethanol extract could be a possible source of compounds with antioxidant and antimicrobial activity.

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